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All Journal Media Kedokteran Hewan Universa Medicina journal of Basic Medical Veterinary Indonesian Andrology and Biomedical Journal
Rahmi Sugihartuti, Rahmi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : journal of Basic Medical Veterinary

 1 
EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Ayu, Zerlinda Dyah; Maslachah, Lilik; Supranianondo, Koesnoto; Suprihati, Endang; Plumeriastuti, Hani; Sugihartuti, Rahmi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091

Abstract

The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments Sugihartuti, Rahmi; Pradana, Munawer; Maslachah, Lilik; Chusniati, Sri; Tyaningsih, Wiwiek; Rahardjo, Dadik; Soeharsono, Soeharsono
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.58266

Abstract

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
Co-Authors Adiana Mutamsari Witaningrum Ayu, Zerlinda Dyah Chusniati, Sri Dadik Rahardjo, Dadik Endang Suprihati Erma Safitri Hani Plumeriastuti Hisyam, Mirza Atikah Madarina IMAM MUSTOFA Iwan Sahrial Hamid Kadek Rachmawati Lilik Maslachah Munawer Pradana Putri, Cindy Ercha Aulia Raharjo, Hartanto Mulyo Rahmatullah, Aldin Akbar Ratnaningtyas, Nurrohmah Saraswati, Afif Tasya Soeharsono Soeharsono Sri Mulyati Suherni Susilowati Supranianondo, Koesnoto Tacharina, Martia Rani Tri Wahyu Suprayogi Tyaningsih, Wiwiek Yulianna Puspitasari