Garuda - Garba Rujukan Digital

IDENTIFICATION OF PTERIDOPHYTES DIVERSITY IN THE MOUNTAINOUS AREA OF BUNTU SARONG VILLAGE, MASALLE DISTRICT, ENREKANG REGENCY Hasmiati Hasmiati; Musma Rukmana; Tika Putri Agustina; Fernando Andre Watung
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : LPPM Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.1010

Ferns (Pteridophyte) are members of the kingdom plantae that contribute to Indonesia's biodiversity, especially the diversity of flora. Ferns have an important role in maintaining the balance of the ecosystem. In addition to having ecological value in the ecosystem as a source of germplasm, ferns also have the potential to be useful to support people's lives. However, in fact ferns are still less well-known and receive less attention than other plants. This is because one of the reasons is that people are less familiar with ferns. This study aims to identify the types of ferns (Pteridophyte) in the mountainous area of Buntu Sarong Village, Masalle District, Enrekang Regency. Data collection was carried out using the exploration method at certain points in the research location that were considered potential, then recording, documenting, identifying and classifying the Pterydophyta found. The research at the research location successfully identified and documented 16 species of Pteridophyte, namely Psilotum nudum, Lycopodium, Selaginella delicatula, Equisetum hyemale, Odontosoria chinensis, Gleichenia linearis, Adiantum sp., Christella parasitica, Aspelinum nidus, Cyathea cooperi, Angiopteris evecta, Deparia petersenii, Microsorum punctatum, Drynaria quercifolia, Microsorum punctatum, Microsorum scolopendria, Drymoglossum piloselloides. The identified Pteridophyte are classified into 13 families (Lycopodiaceae, Psilotaceae, Equisetaceae, Marattiaceae, Lindsaceae, Gleicheniaceae, Adiantaceae, Thelypteridaceae, Asplenidaceae, Chyatheaceae, Athyriaceae, Polypodiaceae) and 5 classes (Lycopodiopsida, Psilotopsida, Equisetopsida, Marattiopsida, Polypodiopsida).

OPTIMIZING FIXATION DURATION FOR ENHANCED CLARITY IN Pediculus humanus-capitis WHOLE MOUNT PREPARATIONS USING 10% KOH AT 70°C Tika putri agustina; Musma Rukmana; Hasmiati; Fernando Andre Watung
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : LPPM Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.1041

This study evaluates the optimal fixation duration using 10% potassium hydroxide (KOH) at 70°C for whole mount preparations of Pediculus humanus capitis (head lice). Accurate head lice identification is crucial for effective public health management. Specimens were fixed for 20, 25, 30, 35, and 40 minutes at 70°C, then processed through rinsing, acetic acid treatment, dehydration, and clearing before mounting for microscopic examination. Results showed that 25 minutes of fixation provided the clearest and most detailed preparations. This duration effectively achieved tissue dehydration and clarification without significant damage. Fixation for 20 minutes was insufficient, resulting in opaque specimens due to incomplete KOH diffusion. Conversely, 40 minutes of fixation caused over-fixation, leading to tissue damage and poorer color quality. The study underscores the importance of finding the right fixation duration. While shorter times are inadequate, longer durations can degrade specimen quality. A 25-minute fixation at 70°C is optimal for balancing clarity and structural preservation, improving head lice identification. This method enhances the quality of whole mount preparations and provides practical guidance for accurate and reliable ectoparasite examination in clinical and research settings.

IDENTIFICATION OF PTERIDOPHYTES DIVERSITY IN THE MOUNTAINOUS AREA OF BUNTU SARONG VILLAGE, MASALLE DISTRICT, ENREKANG REGENCY Hasmiati, Hasmiati; Rukmana, Musma; Agustina, Tika Putri; Watung, Fernando Andre
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.1010

Ferns (Pteridophyte) are members of the kingdom plantae that contribute to Indonesia's biodiversity, especially the diversity of flora. Ferns have an important role in maintaining the balance of the ecosystem. In addition to having ecological value in the ecosystem as a source of germplasm, ferns also have the potential to be useful to support people's lives. However, in fact ferns are still less well-known and receive less attention than other plants. This is because one of the reasons is that people are less familiar with ferns. This study aims to identify the types of ferns (Pteridophyte) in the mountainous area of Buntu Sarong Village, Masalle District, Enrekang Regency. Data collection was carried out using the exploration method at certain points in the research location that were considered potential, then recording, documenting, identifying and classifying the Pterydophyta found. The research at the research location successfully identified and documented 16 species of Pteridophyte, namely Psilotum nudum, Lycopodium, Selaginella delicatula, Equisetum hyemale, Odontosoria chinensis, Gleichenia linearis, Adiantum sp., Christella parasitica, Aspelinum nidus, Cyathea cooperi, Angiopteris evecta, Deparia petersenii, Microsorum punctatum, Drynaria quercifolia, Microsorum punctatum, Microsorum scolopendria, Drymoglossum piloselloides. The identified Pteridophyte are classified into 13 families (Lycopodiaceae, Psilotaceae, Equisetaceae, Marattiaceae, Lindsaceae, Gleicheniaceae, Adiantaceae, Thelypteridaceae, Asplenidaceae, Chyatheaceae, Athyriaceae, Polypodiaceae) and 5 classes (Lycopodiopsida, Psilotopsida, Equisetopsida, Marattiopsida, Polypodiopsida).

OPTIMIZING FIXATION DURATION FOR ENHANCED CLARITY IN Pediculus humanus-capitis WHOLE MOUNT PREPARATIONS USING 10% KOH AT 70°C agustina, Tika putri; Rukmana, Musma; Hasmiati; Watung, Fernando Andre
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.1041

This study evaluates the optimal fixation duration using 10% potassium hydroxide (KOH) at 70°C for whole mount preparations of Pediculus humanus capitis (head lice). Accurate head lice identification is crucial for effective public health management. Specimens were fixed for 20, 25, 30, 35, and 40 minutes at 70°C, then processed through rinsing, acetic acid treatment, dehydration, and clearing before mounting for microscopic examination. Results showed that 25 minutes of fixation provided the clearest and most detailed preparations. This duration effectively achieved tissue dehydration and clarification without significant damage. Fixation for 20 minutes was insufficient, resulting in opaque specimens due to incomplete KOH diffusion. Conversely, 40 minutes of fixation caused over-fixation, leading to tissue damage and poorer color quality. The study underscores the importance of finding the right fixation duration. While shorter times are inadequate, longer durations can degrade specimen quality. A 25-minute fixation at 70°C is optimal for balancing clarity and structural preservation, improving head lice identification. This method enhances the quality of whole mount preparations and provides practical guidance for accurate and reliable ectoparasite examination in clinical and research settings.

Analysis Of The Potential Of Natural Sterol Compounds From Tin (Ficus carica) Leaves as Anti-Hypercholesterolemia with In Silico Tests Taihuttu, Monalisa Pertiwi Jeriska; Manurung, Brian Saputra; Simal, Rufiati; Watung, Fernando A.
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.11961

This study aims to determine the potential of natural sterol compounds from Tin (Ficus carica) leaves as anticholesterolemic using in silico test. This study employed an in silico approach using molecular docking methods to evaluate the potential of sterol compounds as anti-hypercholesterolemic agents, in comparison to other references compounds. Data processing and interpretation were conducted using molecular databases for both ligands and target proteins. The analysis utilized several computational tools, including PyRx 0.8, PyMOL, LigPlus, and Discovery Studio 2016 Client. The compounds used in this research were sterol, fluvastatin and simvastatin (as control), with HMG CoA reductase as the target protein. The results showed that the highest binding affinity value was fluvastatin which is -8.3 kcal/mol. Sterol compounds are compounds with lower binding affinity which is -7.7 kcal/mol. From the visualization results, it is known that the binding distance between sterol compounds with target proteins (HMG CoA reductase) is between 2.69 to 5.49 Å, and the binding distance between simvastatin compounds with target proteins (HMG CoA reductase) is between 2.76 to 5.36 Å, and the binding distance between fluvastatin compounds with target proteins (HMG CoA reductase) is between 1.95 to 5.26 Armstrong. While based on the comparison of the binding side of strerol, simvastatin and fluvastatin, it is known that the three compounds have the same site because they bind to the same amino acid residues, namely ARG (B: 515), TYR (A: 533), TYR (B: 533), TYR (B: 517), PRO (A: 511). The results of this research indicate that the natural sterol compounds found in Tin (Ficus carica) leaves have potential as anti-hypercholesterolemic agents, based on reverse docking analysis. These sterol compounds bind to the same active site as the control drugs, simvastatin and fluvastatin, and exhibit comparable binding affinity values

Isolasi dan Identifikasi Bakteri Penyebab Penyakit pada Ikan Nila (Oreochromis niloticus) di Desa Tatelu Kecamatan Dimembe Kabupaten Minahasa Utara Watugigir, Gerfindo Y. G; Rayer, Debby J. J.; Lawalata, Helen J; Watung, Fernando A; Rampengan, Mercy M. F
SOSCIED Vol 7 No 2 (2024): SOSCIED - November 2024
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v7i2.870

Tilapia is a freshwater animal that is always in contact with the aquatic environment so that it is easily infected by bacteria through water. This study aims to identify the types of bacteria that cause diseases that attack Tilapia (Oreochromis niloticus) kept in Dimembe District, North Minahasa Regency. This study uses a descriptive exploratory research method. The results of the study found sixteen isolates and identified them as bacteria that cause Tilapia disease in North Minahasa Regency, including Aeromonas sp. (4 isolates), Plesiomonas sp. (5 isolates), and Enterobacter sp. (1 isolate). based on morphological characteristics (colonies and bacterial cells) and biochemical (catalase, oxidase and motility tests). It was found that periodic and continuous fish disease surveillance is needed to monitor the spread of fish diseases, especially Tilapia disease in North Minahasa

IDENTIFICATION OF MACROALGAE IN THE COASTAL AREA OF BUDO TOURISM VILLAGE, WORI, NORTH MINAHASA Watung, Fernando; Supit, Annely; Setyawati, Iriani; Kamagi, Decky; Agustina, Tika Puri; Rukmana, Musma; Hasmiati, Hasmiati
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10837

Algae are lower plants that do not have differentiated structures such as roots, stems, and leaves; although they may appear to have differences, they are actually just thallus forms. Macroalgae have macroscopic body shape and size consisting of fronds without distinct roots, stems, and leaves. Macroalgal diversity is not only reflected in their ecological and economic significance but also in their high species richness and wide geographical distribution within aquatic ecosystems. However, research on the species diversity of macroalgae in Budo Tourism Village Beach, North Minahasa remains limited. This study aims to identify the macroalgal species in the Budo Tourism Village, Wori, North Minahasa area. The research employed the line transect and quadrat-based sampling method, which was implemented at two distinct station locations: Station I (rocky reef substrate) and Station II (sandy-muddy substrate). Macroalgae identification was facilitated by utilizing the comprehensive identification textbooks. The research reveals the presence of four macroalgal species, comprising three brown algae (Phaeophyceae), namely Padina boergesenii, Sargassum llinearifolium, Turbinaria ornata and one red algae (Rhodophyceae), Gracilaria corticata. Sargassum linearifolium, Padina boergesenii, and Gracilaria corticata was found in rocky reef substrate. Turbinaria ornate and Padina boergesenii was found in sandy-muddy substrate.

Analysis Of The Potential Of Natural Sterol Compounds From Tin (Ficus carica) Leaves as Anti-Hypercholesterolemia with In Silico Tests Taihuttu, Monalisa Pertiwi Jeriska; Manurung, Brian Saputra; Simal, Rufiati; Watung, Fernando A.
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.11961

This study aims to determine the potential of natural sterol compounds from Tin (Ficus carica) leaves as anticholesterolemic using in silico test. This study employed an in silico approach using molecular docking methods to evaluate the potential of sterol compounds as anti-hypercholesterolemic agents, in comparison to other references compounds. Data processing and interpretation were conducted using molecular databases for both ligands and target proteins. The analysis utilized several computational tools, including PyRx 0.8, PyMOL, LigPlus, and Discovery Studio 2016 Client. The compounds used in this research were sterol, fluvastatin and simvastatin (as control), with HMG CoA reductase as the target protein. The results showed that the highest binding affinity value was fluvastatin which is -8.3 kcal/mol. Sterol compounds are compounds with lower binding affinity which is -7.7 kcal/mol. From the visualization results, it is known that the binding distance between sterol compounds with target proteins (HMG CoA reductase) is between 2.69 to 5.49 Å, and the binding distance between simvastatin compounds with target proteins (HMG CoA reductase) is between 2.76 to 5.36 Å, and the binding distance between fluvastatin compounds with target proteins (HMG CoA reductase) is between 1.95 to 5.26 Armstrong. While based on the comparison of the binding side of strerol, simvastatin and fluvastatin, it is known that the three compounds have the same site because they bind to the same amino acid residues, namely ARG (B: 515), TYR (A: 533), TYR (B: 533), TYR (B: 517), PRO (A: 511). The results of this research indicate that the natural sterol compounds found in Tin (Ficus carica) leaves have potential as anti-hypercholesterolemic agents, based on reverse docking analysis. These sterol compounds bind to the same active site as the control drugs, simvastatin and fluvastatin, and exhibit comparable binding affinity values

IDENTIFICATION OF MACROALGAE IN THE COASTAL AREA OF BUDO TOURISM VILLAGE, WORI, NORTH MINAHASA Watung, Fernando; Supit, Annely; Setyawati, Iriani; Kamagi, Decky; Agustina, Tika Puri; Rukmana, Musma; Hasmiati, Hasmiati
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10837

Algae are lower plants that do not have differentiated structures such as roots, stems, and leaves; although they may appear to have differences, they are actually just thallus forms. Macroalgae have macroscopic body shape and size consisting of fronds without distinct roots, stems, and leaves. Macroalgal diversity is not only reflected in their ecological and economic significance but also in their high species richness and wide geographical distribution within aquatic ecosystems. However, research on the species diversity of macroalgae in Budo Tourism Village Beach, North Minahasa remains limited. This study aims to identify the macroalgal species in the Budo Tourism Village, Wori, North Minahasa area. The research employed the line transect and quadrat-based sampling method, which was implemented at two distinct station locations: Station I (rocky reef substrate) and Station II (sandy-muddy substrate). Macroalgae identification was facilitated by utilizing the comprehensive identification textbooks. The research reveals the presence of four macroalgal species, comprising three brown algae (Phaeophyceae), namely Padina boergesenii, Sargassum llinearifolium, Turbinaria ornata and one red algae (Rhodophyceae), Gracilaria corticata. Sargassum linearifolium, Padina boergesenii, and Gracilaria corticata was found in rocky reef substrate. Turbinaria ornate and Padina boergesenii was found in sandy-muddy substrate.

Development of General Biology Learning E-Modules Based on Constructivism Rukmana, Musma; Andre Watung, Fernando; Hasmiati, Hasmiati; Putri Agustina, Tika; Restutiningsih Putri Utami, Aisyiah
Scholaria: Jurnal Pendidikan dan Kebudayaan Vol 14 No 2 (2024)
Publisher : Program Studi Pendidikan Guru Sekolah Dasar, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/j.js.2024.v14.i2.p167-176

Higher education must adapt its educational practices to ICT, which is now developed rapidly. There are many different approaches, and constructivism-based e-module development is an alternative. This research used the R&D method by adapting the ADDIE development model, which aimed to produce constructivism-based e-module products that meet the criteria of being valid, effective, and practical for use in the learning process. The e-module developed was validated using an expert validation questionnaire filled out by two expert validators, with a result of 4.32, which was included in the valid category. The effectiveness of the e-module was obtained by 100% of students achieving learning completeness scores and increasing learning outcomes based on a normalized gain analysis of 0.71 (high category). The results of the e-module practicality test showed positive student responses, where 86.67% of statements were included in the very strong category and 13.33% in the strong category. Based on the research results, it can be concluded that the constructivism-based general biology learning e-module developed is valid, practical, and effective.

Title

Found 21 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 21 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 21 Documents
Search