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The Combination of Plant Growth Promoting Rhizobacteria (PGPR) Paitan Root with Several Types of Bokashi and Its Effect on Tatsoi Meyuliana, Aulia; Yora, Muharama; Elinda, Friza; Miranda, Cut Nyak Dara; Sihotang, Saipul
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 1 (2024): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (1)Desember 2024
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i1.12957

Abstract

The combination of PGPR (Plant Growth Promoting Rhizobacteria) with bokashi aims to increase nutrient availability in the soil. PGPR which comes from paitan roots is known to have the potential to help nutrient availability so by combining it with bokashi it is hoped that it will optimize its potential in increasing tatsoi production. The bokashi used in this research were chicken manure bokashi and goat manure bokashi. Research design with two factors, namely five doses of PGPR and two types of bokashi. The PGPR doses used were 0 ml/L (D0), 50 ml/L (D1), 100 ml/L (D2), 150 ml/L (D3), 200 ml/L (D4) and two types of Chicken bokashi (A) and Goat Bokashi (K) with 5 repetitions. The observations measured were plant height (cm), number of leaves (strands) and crop diameter (cm). The results of the research showed that the combination of PGPR with bokashi chicken manure and bokashi goat manure did not have a significant effect on plant growth, namely plant height, number of leaves and crop diameter of tatsoi.
Pengaruh Mikoriza dan POC Urin Sapi terhadap Pertumbuhan Cabai Merah di Zona Iklim Kering E Parlindungan Sitepu, David; Sihotang, Saipul
Jurnal Ilmiah Pertanian ( JIPERTA) Vol 7, No 2 (2025): Jurnal Ilmiah Pertanian (JIPERTA), September
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jiperta.v7i2.6009

Abstract

Red chili pepper (Capsicum annuum L.) is a horticultural commodity with high economic value that is greatly influenced by agroclimatic conditions. In dry climate zone E (Oldeman classification), plant growth is often hampered due to water limitations and low soil fertility. This study aims to evaluate the effect of arbuscular mycorrhizal administration and liquid organic fertilizer (POC) of cow urine on the vegetative growth of red chili plants in dry climate zone E. The study was conducted using a factorial Random Group Design (RAK) with two factors: mycorrhizal dose (0, 35, 85, and 135 g/plant) and POC concentration of cow urine (0, 30, 35, and 40 ml/L), Each was repeated three times, making a total of 48 experimental units. The parameters observed included plant height, number of leaves, stem diameter, time of emergence and number of shoots. The results showed that the dose of mycorrhizal 135 g and the POC concentration of 40 ml/L gave the best results on almost all vegetative parameters. The interaction of the two also showed a significant influence on plant height and number of shoots. These findings support the application of bioagent-based eco-friendly technologies in sustainable farming systems on dry climates.
Potensi Aplikasi Endofit dalam Agroekosistem Berkelanjutan: Biofertilizer dan Biokontrol Hayati Sihotang, Saipul; Panggabean, Ellen Lumisar; Alifyah, Nabila; Saragih, Nur Betty; Nasution, Lita; Halawa, Emmanuel; Saragih, Ryan
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 2 (2025): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (2) Juni 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i2.7859

Abstract

Endophytic microorganisms have become a central focus in numerous studies related to sustainable agriculture due to their dual potential as biological agents—functioning both as biocontrol and biofertilizers. The presence of endophytes within plant tissues has been shown to enhance vegetative growth, strengthen resistance against pathogenic attacks, and optimize nutrient uptake. This article presents a systematic review of recent studies from 2018 to 2024, addressing the classification, mechanisms of action, and potential applications of endophytic microorganisms in agriculture. Several bacterial genera, including Bacillus spp., Azospirillum spp., and Pseudomonas spp., along with non-pathogenic fungal groups such as Trichoderma spp. and Fusarium spp., have demonstrated effectiveness in nitrogen fixation, phosphate solubilization, phytohormone synthesis, and the production of antimicrobial compounds. Research findings indicate that the application of endophytic microorganisms as biological inputs holds great promise in supporting the development of environmentally friendly, sustainable, and high-yielding agricultural systems.
Biofertilizer For Increasing Growth And Yield Of Melon (Cucumis Melo L.) Alifyah, Nabila; Betty Saragih, Nur; Maulana, Bagus; Sihotang, Saipul
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 9 No. 2 (2025): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 9 (2) Juni 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v9i2.12584

Abstract

This study aims to develop biofertilizer from Metharizium sp. to increase the production of melon plants (Cucumis melo L.). The excessive use of chemical fertilizers has damaged soil quality and the environment in Indonesia. Environmentally friendly biofertilizers can be a solution to improve soil fertility and increase crop yields. The research was conducted in Serdang Bedagai Regency from April to July 2024. The research method includes land preparation, planting, making granular compost, fertilizer application, and data analysis. The study is expected to provide an effective Metharizium sp. granular fertilizer formulation to increase melon production and reduce the use of chemical fertilizers.
IDENTIFIKASI KANDUNGAN SENYAWA METABOLIT SEKUNDER PADA BATANG DAN DAUN SURAT DIBATA (Macodes petola) MENGGUNAKAN GAS CHROMATOGRAPHY SPECTROMETRY (GCMS) Sianturi, Parulian; Dalimunthe, Nur Asyiah; Sahfitra, Angga Ade; Aziz, Rizal; Sihotang, Saipul
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 8 No. 2 (2024): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v8i2.12725

Abstract

This study, entitled Identification of Secondary Metabolite Compounds in the Stems and Leaves of Surat Dibata (Macodes petola) Using Gas Chromatography Mass Spectrometry (GCMS) aims to determine the compounds and their concentrations of secondary metabolites present in the stems and leaves of Surat Dibata (Macodes petola) using Gas Chromatography Mass Spectrometry (GCMS). The methods used in this study include maceration extraction to identify secondary metabolite compounds and Gas Chromatography Mass Spectrometry (GCMS) to determine the concentrations of these compounds in the stems and leaves of Surat Dibata (Macodes petola). The results of the study showed that the extraction identified secondary metabolite compounds such as steroids in the stems and flavonoids, steroids, and tannins in the leaves. Based on the results from the GCMS analysis, the stems were found to contain 0.63% steroids, while the leaves contained 0.002% flavonoids, 0.002% tannins, and 2.215% steroids.
Isolation, Morphological Characterization, Biochemistry, and Antagonistic Activity of Endophytic Bacteria from Rice Against Xanthomonas oryzae pv. Oryzae Saipul Sihotang; Alfons Dwi Putra Butar-Butar; Lita Nasution; Haliatur Rahma; Trizelia Trizelia; Magdalena Saragih; Retna Astuti Kuswardani; Ika Listiana; Beatrix Sofranes Napitupulu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.825-829

Abstract

Endophytic bacteria have the potential as environmentally friendly biocontrol agents in controlling bacterial leaf blight (BLB) in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). This study aims to isolate, characterize morphology and biochemical tests, and test the antagonistic activity of endophytic bacteria from rice against Xoo. Isolation was carried out from healthy rice root tissue using a surface sterilization method. Morphological characterization included colony shape, color, edge, elevation, and Gram properties, while biochemical characterization included catalase, oxidase, motility, starch hydrolysis, and sugar fermentation tests. The antagonistic test used a dual culture assay method on NA (nutrient agar) media. The isolation results obtained six endophytic bacterial isolates (BETP 01–06) identified as the genus Pseudomonas and Bacillus with varying colony morphological characters. Antimicrobial activity test showed two potential isolates, BETP 03 (Pseudomonas) and BETP 05 (Bacillus), with an average inhibitory diameter of 16.3 mm and 16.5 mm against Xoo, respectively. Biochemical characterization showed that BETP 03 was bacillus, Gram-negative, citrate-negative, motile, gelatin hydrolysis positive, starch hydrolysis positive, and catalase positive; while BETP 05 was bacillus, Gram-positive, citrate-positive, motile, gelatin hydrolysis negative, starch hydrolysis positive, and catalase positive. These results indicate that both isolates have high potential as biological biocontrol agents in controlling bacterial leaf blight disease in rice.
Effects of Naphthaleneacetic Acid and Kinetin on Chrysanthemum Plantlets in Vitro Saipul Sihotang; Magdalena Saragih; Swati Sembiring; Riduan Sembiring; Dini Puspita Yanty; Denny Akbar Tanjung; Elli Afrida
Journal of Applied Agricultural Science and Technology Vol. 9 No. 4 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i4.315

Abstract

Naphthaleneacetic acid (NAA) and kinetin play a crucial role in plant growth but must be used in correct proportions in order to produce the best outcomes. An optimal combination of both can enhance shoot, leaf, and root growth, whereas inappropriate doses may otherwise inhibit growth due to hormonal imbalance. This study examined the effects of the interaction between NAA and kinetin on the growth of chrysanthemum plants at eight weeks of age. The research was carried out using the Completely Randomized Design (CRD) in triplicate. The medium used was MS medium, supplemented with NAA at concentrations of 0–1.5 mg/L and kinetin at concentrations of 0–3 mg/L. The results indicate that NAA and kinetin positively influenced plant growth, but only when the optimal concentrations were applied. The best combination of NAA at 1.0 mg/L (N2) and kinetin at 3.0 mg/L (K3) produced the best results in terms of height (153 mm), number of leaves (31 leaves), number of roots (26 roots), and fresh weight (5.85 g). Increasing kinetin concentrations (K0-3) generally promoted plant growth, whereas excessive NAA concentrations (> 1.0 mg/L, N3) produced the opposite effect, likely due to toxicity or hormonal imbalance. The interaction between kinetin and NAA exhibited a synergistic effect up to a certain threshold, while excessive NAA application negatively impacted on plant development.
Utilization of Various Vegetable Insecticides to Control Grayak Caterpillars (Spodoptera litura) on Soybean (Glycine max L. Merrill) in Laboratory Azwana Azwana; Saipul Sihotang
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3181

Abstract

The aim of this study was to observe the effect of various vegetable insecticide extracts on controlling armyworm (Spodoptera litura) on soybean plants in the laboratory. The research was conducted at the Plant Protection Laboratory, Faculty of Agriculture, University of Medan Area, from November 2021 to April 2022, using a Completely Randomized Design (CRD) Non Factorial, consisting of 7 treatment levels, namely: N0 = no treatment (control), N1 = Mindi leaf extract concentration 5% (50 m/l water), N2 = Mindi leaf extract 10% (100 ml.l water), N3 = Mindi leaf extract concentration 15% (150 m/l water), N4 Babadotan concentration 5 % (50 ml/l air), N5 = 10% concentration of Babadotan leaf extract (100 ml/lair), N6 = 15% concentration of Babadotan leaf extract (150 ml/lair), with repetition 3. Parameters observed for Armyworm Mortality (%), LC50, LT50, the proportion of the effectiveness of vegetable insecticides on armyworm mortality and the amount of feed consumed. The results showed that the application of vegetable insecticides had a significant and very significant effect on armyworm mortality from 4 days after application to 10 days after application with the highest mortality proportion at a concentration of 15%. The LC50 values of the plant insecticides mindi leaves and babadotan leaves were almost the same, namely 4.69 and 4.48%. The LT 50 value for a concentration of 15% showed that babadotan leaf extract was faster than mindi leaf extract, namely mindi leaves 5.145 days and babadotan leaves 4.633 days. Concentrations of 10 and 15 % of the 2 plant extracts tested showed the same effectiveness on mortality of S. litura caterpillars and higher dissolving concentrations of 5%. The amount of feed consumed was also significantly different with the highest amount of feed in the control treatment (60.75 g) followed by the treatment of mindi leaves and babadotan leaves at concentrations of 15, 10, and 5%.
Analysis of Organic Mulching on Soil Fertility and the Productivity of Sweet Corn (Zea mays saccharata Sturt L.) Siti Mardiana; Sayyid Al-Fadhil Hasibuan; Sumihar Hutapea; Saipul Sihotang
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4528

Abstract

One of the causes of the low productivity of sweet corn is decreased soil productivity due to the leaching of nutrients by rainwater, tillage, and continuous application of inorganic fertilizers. The function of mulching is to increase soil moisture, suppress weed growth, and prevent soil erosion to create optimal soil conditions for plant growth. This study aimed to determine the effect of organic mulching on soil fertility and the productivity of sweet corn (Zea mays saccharata Sturt). This study used a Split Plot Design consisting of two factors, Tillage (P) and Organic Mulch (M) which had 6 levels, M0 = No Mulch, M1 = Plastic Mulch, M2 = Rice Straw Mulch, M3 =Cocopeat Mulch, M4 = Sawdust Mulch, M5 = Rice Husk Mulch. The results show that the M2 treatment of Rice Straw Mulch tended to increase soil fertility and had a significant effect on the productivity of sweet corn.
Factors Affecting the Income of Labor Fishermen (Case Study: Tebing Tinggi Village, Tanjung Beringin District, Serdang Bedagai Regency) Rizal Aziz; Roni Paulus Sihombing; Rahma Sari Siregar; Saipul Sihotang
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4530

Abstract

Indonesia is a country that has a very large water area which is a large potential resource to be utilized for national development. This condition affects the strong weak economic activities of the village. The purpose of the study is to find out how much the income of labor fishermen in Tebing Tinggi Village, Tanjung Beringin District, Serdang Bedagai Regency and to find out what factors affect the income of labor fishermen in Tebing Tinggi Village, Tanjung Beringin District, Serdang Bedagai Regency. This research was conducted in Tebing Tinggi Village, Tanjung Beringin District, Serdang Bedagai Regency, North Sumatra Province. This location was chosen intentionally (Purposive). The population in this study was 546 labor fishermen, the determination of the sample in this study used Arikunto's theory, namely if the total population was less than 100 people, then the number of samples was taken as a whole. But if the population is larger than 100 people then it can be taken 10-15% and 20-25% of the total population. The samples in this study were taken as much as 10% of the total population, so the number of samples in this study was 55 labor fishermen. Data collection techniques use primary data and secondary data, data analysis techniques in research use income analysis and multiple linear regression analysis. The results of the research on the working capital costs of labor fishermen amounted to Rp. 244,894/month, with an income of Rp. 4,128,386/month with an average of 12 trips a month of fishing. The results of multiple linear regressions that the working capital variable and the catch variable have a significant effect on the income of labor fishermen while the time variable at sea and experience variables do not have a significant effect on the income of labor fishermen in Tebing Tinggi Village, Tanjung Beringin District, Serdang Bedagai Regency.
Co-Authors Adi Fathul Qohar Agus Susanto Alfons Dwi Putra Butar-Butar Alifyah, Nabila Angga Ade Sahfitra Anggun Sianipar Asmah Indrawati Asmah Indrawaty Aulia Meyuliana Azwana Azwana Beatrix Sofranes Napitupulu Betty Saragih, Nur Cut Nelly Dedi Holden Simbolon Denny Akbar Tanjung Desi Sri Pasca Sari Sembiring, Desi Sri Pasca Sari Dini Puspita Yanty Elinda, Friza Ellen Lumisar Panggabean Elli Afrida Emmanuel Halawa Erwin Pane Frisky Purba Ginting, Rajiv Gracia Christy Sitompul Halawa, Emmanuel Haliatur Rahma, Haliatur Ika Listiana Imam Alfazri Indera Prabowo Pandiangan Irfan Suliansyah Ivan Permana Putra Jacqueline Joseph Jamsari Jamsari Juni Kasih Lombu Lilis Supratman Mada Triandala Sibero Magdalena Saragih Magdalena Saragih Marco Y. F Sitompul Maulana, Bagus Merlan Manurung Merlyn Mariana Miranda, Cut Nyak Dara Mochammad Imron Awalludin Muhammad Aldy Muhammad Ilham Muhammad Usman Nasution, Lita Natalina Tarigan Nur Asyiah Dalimunthe Oktan Dwi Nurhayat Pandiangan, Indera Prabowo Parlindungan Sitepu, David Rahma Sari Siregar Rajiv Ginting Renfiyeni Retna Astuti Kuswardani Riduan Sembiring Rikson Asman Fertiles Siburian Rita Fitriani Rizal Aziz Rizal Aziz, Rizal Roni Paulus Sihombing Rudy Hermawan SAISA SAISA Saragih, Magdalena Saragih, Nur Betty Saragih, Ryan Sartini Sartini Sayyid Al-Fadhil Hasibuan Sembiring, Riduan Sembiring, Swati SERI DEPI Shara Nafisah Sianturi, Parulian Silaban, Sri Rejeki Siti Mardiana SITI MARDIANA Siti Mardiana Sri Rejeki Silaban Srie Faizah Lisnasari Sumihar Hutapea Suswati Suswati Suswati Swati Sembiring Trizelia Yabani Yabani Yora, Muharama Yosafat Siregar Zulheri Noer