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All Journal Pharmaciana: Jurnal Kefarmasian THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal Media Analis Kesehatan Indonesian Journal of Halal Research Jurnal EduHealth Kieraha Medical Journal Indonesian Journal of Community Empowerment Health Science & Biomedical Journal (HSBJ)
Nurul Azmah Nikmatullah
Universitas Muhammadiyah Prof. Dr. Hamka & Halal Studies Center Universitas Muhammadiyah Prof. Dr. Hamka
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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PERSONAL HYGIENE TERHADAP INFEKSI BAKTERIAL VAGINOSIS PADA WANITA DENGAN FLOUR ALBUS Nurul Azmah
Kieraha Medical Journal Vol 3, No 2 (2021): KIERAHA MEDICAL JOURNAL
Publisher : Fakultas Kedokteran, Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.589 KB) | DOI: 10.33387/kmj.v3i2.3950

Abstract

Bakterial vaginosis merupakan infeksi vagina yang umum terjadi pada wanita dengan seksual aktif. Faktor penyebab keluhan pada vagina terbanyak diakibatkan infeksi vagina yang disebabkan oleh bakteri, jamur, virus dan parasit. Kumpulan gejala yang timbul pada bacterial vaginosis berhubungan dengan aktivitas seksual. Infeksi yang terjadi pada vgina dapat menyebabkan flour albus. Personal hygiene diperlukan untuk kenyamanan dan kesehatan individu. karena kurangnya kesadaran personal hygiene pada organ kewanitaan dapat menyebabkan flour albus. Tujuan penelitian ini untuk mendapatkan hubungan personal hygiene terhadap kejadian infeksi bacterial vaginosis pada wanita dengan flour albus. Pemeriksaan dilakukan dengan melakukan swab pada vagina pada perempuan usia reproduktif dengan keluhan flour albus, hasil swab akan diperiksa secara mikroskopis dengan pewarnaan gram untuk melihat adanya bakteri penyebab flour albus.Hasil penelitian ditemukan bakteri batang garam negatif , didapakan 17 responden (11,3%) yang mengalami bakterial vaginosis, dan setelah dianalisis menggunakan R square, terdapat hubungan personal hygiene dengan kejadian bakterial vaginosis.
DETEKSI CEMARAN TIKUS PADA SAMPEL SOSIS DENGAN REAL TIME PCR Hadi Sunaryo; Nurul Azmah Nikmatullah; Samiratul Mufidah
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol. 9 No. 2 (2022)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/farmasains.v9i2.7960

Abstract

Dalam ajaran Islam daging tikus merupakan bahan pangan yang tidak halal atau tidak diperbolehkan untuk dikonsumsi, sehingga kehadirannya dalam suatu olahan makanan merupakan isu yang sangat penting. Olahan daging sering kali dipalsukan dengan penambahan daging tikus, hal ini dilakukan untuk menekan biaya produksi olahan daging. Oleh karena itu, penelitian ini bertujuan untuk mengidentifikasi cemaran Tikus pada sampel sosis menggunakan metode Real Time Polymerase Chain Reaction (RT-PCR). Identifikasi ini terbagi menjadi 2 tahap yaitu tahapan ekstraksi menggunakan kit Progenus EasyFastTM Extraction Kit for Meat Products dan tahapan amplifikasi menggunakan kit EASYFASTTM Rat detection kit. Pada penelitian ini sampel yang diidentifikasi sebanyak 30 sampel. Dari 30 sampel yang telah diidentifikasi terdapat 1 sampel yang positif mengandung cemaran DNA tikus dengan nilai Ct 36,16
OPTIMASI METODE FLOTASI SENTRIFUS MENGGUNAKAN LARUTAN ZnSO₄, MgSO₄, DAN NaCl BERDASARKAN KONSENTRASI LARUTAN DAN LAMA PENGAPUNGAN Alma Tiara Rahayu; Aldi Pratama; M. Wahyu Setiawan; Ma'rifatussolihat Ma'rifatussolihat; Nurul Azmah Nikmatullah
Jurnal Media Analis Kesehatan Vol 14, No 1 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v14i1.3283

Abstract

In Indonesia, helminth infection is one of the diseases with a fairly large number. Soil Transmitted Helminth (STH) is the transmission of helminth infection through the soil. Examination of feces is carried out to diagnose helminth infections by finding infective eggs or larvae. Deworming analysis can be done quantitatively or qualitatively. The flotation, sedimentation and native (direct slide) method is a qualitative worm infection examination method to detect intestinal parasites, while the Kato-Katz method is a quantitative method of detecting helminth infections. Purpose: to find out the optimal concentration and duration of flotation in the flotation method using solutions of ZnSO, MgSO and NaCl. The method used is the centrifugal flotation method. The samples were screened and 12 positive samples were obtained. Results: found egg type of worm Ascaris lumbricoides with fertile, infertile and decorticated forms. At a concentration of 33% 10 minutes, a solution of ZnSO 53 eggs was obtained, MgSO 16 eggs, NaCl 10 eggs while 20 minutes ZnSO 29 eggs, MgSO 39 eggs, NaCl 19 eggs were obtained. At a concentration of 43% time 10 minutes, ZnSO 29 eggs were obtained, MgSO 17 eggs, NaCl 11 eggs while a time of 20 minutes was obtained ZnSO 34 eggs, MgSO 26 eggs, NaCl 1 egg. Conclusion: the optimal concentration and flotation time in ZnSO solution is a concentration of 33% with a time of 10 minutes, mgSO solution with a concentration of 33% for 20 minutes, and a NaCl solution with a concentration of 33% for 20 minutes. 
Efektifitas Larutan ZnSO4 33% dan Sukrosa 33% Pada Pemeriksaan Soil Transmitted Helminth Menggunakan Metode Flotasi Sentrifugasi Nurul Azmah Nikmatullah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.18289

Abstract

Soil-Transmitted Helminth (STH) infection is an infection caused by Ascaris lumbricoides worms, Ancylostoma duodenale worms, Necator americanus worms, and Trichuris trichiura worms. Transmission of infection occurs through soil media containing feces contaminated with STH worm eggs. STH worm infection can be identified by qualitative and semi-quantitative stool examination. Stool qualitative examination consists of the direct slide method (native), the flotation method, and the sedimentation method. This study aims to see the effectiveness of 33% ZnSO4  solution and 33% sucrose solution in examining STH worm eggs in the centrifugation flotation method. This study began by collecting feces samples; then the samples were identified by using the flotation centrifugation method. The solutions used in this method were 33% ZnSO4 solution and 33% sucrose solution. The data obtained were analyzed by using the Mann-Whitney test. There were 35 stool samples identified. The examination results obtained 13 positive samples of Ascaris lumbricoides worm eggs in 33% ZnSO4  solution and 1 positive sample in 33% sucrose solution, and the results of the Mann Whitney test obtained Asymp. Sig <0.05. Therefore, there is a significant difference between the 33% ZnSO4 solution and the 33% sucrose solution. In conclusion, the most effective solution used in the flotation method is 33% ZnSO4.   Keywords: Soil-Transmitted Helminth Infection, Centrifugation Flotation, 33% ZnSO4 Solution, 33% Sucrose Solution
Earthworm Infusion as an Antimicrobial Against Bacteria Causing Diarrhea Masyhuda, Irmawati; Nikmatullah, Nurul Azmah
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v17i2.6649

Abstract

Diarrhea is the second most common disease worldwide after Acute Respiratory Infection (ARI). One of the causes of diarrhea is the gastrointestinal infection caused by disease bacteria (microbe) such as Salmonella sp., Escherichia coli, Shigella sp., and Vibrio cholerae. The earthworm species Lubricus sp can be used as an alternative medication believed to be able to cure digestive diseases such as typhus, dysentery, and other indigestion like gastritis. This research is laboratory experimental research. The antibacterial activity is measured by determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of earthworm infusion (Lumbricus rubellus) using the tube dilution test. The bacteria used in this research are Escherichia coli, Shigella dysentriae, and Vibrio cholerae, isolated from the feces of diarrhea patients. This research aims to examine the earthworm infusion antibacterial (Lumbricus rubellus) activity to several bacteria that commonly cause diarrhea. The research results show that the average MIC value of earthworm infusion (Lumbricus rubellus) against Escherichia coli is >50%, Shigella dysentriae 0,098%, and Vibrio cholerae >50%. The average MBC value of earthworm infusion against Shigella dysentriae is 50%. .
The Correlation Between Nutritional Status, Age, and Gender on the Incidence of Soil-Transmitted Helminth Infections Nikmatullah, Nurul Azmah; Masyhuda, Irmawati; Permanasari, Etin Diah; Rahmadilla, Chintya
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 17 No 2 (2023): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v17i2.6896

Abstract

Soil-transmitted helminth (STH) infections most commonly infect preschool and school-age children. The prevalence of STH infection remains high, i.e., 73 million children are infected in Indonesia. The most commonly infecting worms are roundworms (Ascaris lumbricoides), whipworms (Trichuris trichiura), and hookworms (Ancylostoma duodenale and Necator americanus). STH infection is transmitted through soil contaminated by STH eggs or larvae. Personal hygiene, availability of clean water, and defecation behavior are factors in the development of STH infection. Prolonged infection can cause malnutrition, anemia, and slow growth. This study aims to examine the association between nutritional status, age, and gender on the incidence of STH infection. This research is a descriptive-analytical study with a cross-sectional approach. Fifteen (42.86%) of 35 preschool and school-age children's stool samples were positive for Ascaris lumbricoides eggs. The nutritional status of the children was grouped into underweight, normal, and overweight. The body mass index (BMI) calculation resulted in 65.71% (23/35) underweight, 31.43% (11/35) normal, and 2.86% (1/35) overweight children. The Spearmen test shows that there is no significant correlation between nutritional status (p=0.517), age (p=0.614), and gender (p=0.637) on STH infection.
Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time Sunaryo, Hadi; Wirman, Adia Putra; Permanasari, Etin Diah; Nikmatullah, Nurul Azmah; Lestari, Dian; Nurjanah, Desi
Indonesian Journal of Halal Research Vol 5, No 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21325

Abstract

DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins. Contamination of RNA, carbohydrates, lipids, and proteins can increase DNA purity. DNA purity was measured using a NanoDrop 2000 spectrophotometer measured by the absorbance ratio at 260 nm and 280 nm wavelengths. Good quality DNA will have an A260/A280 ratio of 1.7–2.0 and a concentration > 0.03 pg. This study aimed to obtain the appropriate DNA extraction method for fresh meat samples (a mixture of rat and chicken meat). This research consisted of two stages: the DNA extraction stage using the Progenus EasyFast™ Extraction Kit for Meat Products and the amplification stage using the EASYFAST™ Rat Detection Kit. This study used 16 samples of a mixture of rat meat and chicken with concentrations of rat meat: 5, 10, 15, and 20%. At the extraction stage, the incubation time was optimized for 15, 30, 45 minutes, and 1 hour. The results showed that the one hour incubation had a lowest CT value in the results of PCR amplification.
DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method Permanasari, Etin Diah; Sunaryo, Hadi; Wirman, Adia Putra; Rahmadini, Nuriza; Aggasy, Savira Yustinah; Nikmatullah, Nurul Azmah
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.27850

Abstract

The meat-based products are highly susceptible to counterfeiting, primarily due to high consumer demand of meat derivative products, such as meatballs. This demand creates opportunities for food fraud by specific industries, including adulterating meat with non-halal species, such as rats. This research aimed to detect rat meat contamination in meatball samples from the Indonesian local market using Real-time Polymerase Chain Reaction (RT-PCR). The RT-PCR amplification involved an initial denaturation step at 95°C for 3 minutes, followed by denaturation at 95°C for 15 seconds, and annealing/extension at 60°C for 1 minute. The rat-specific probe primer included in the kit produced an increasing curve in the External Positive Control (EPC) with a Ct value of 27.22, and no amplification occurred in the Negative Control (NTC). The analysis of 30 samples from meatball vendors yielded negative results, as there was no increase in the FAM (rat) curve, indicating that none of the meatballs were contaminated with rat DNA.
PREVALENSI JENIS INFEKSI GENITALIA PADA IBU HAMIL TRIMESTER III DI KECAMATAN JATINEGARA Purbasari, Euis; Erikardo, Oktadio; Nikmatullah, Nurul Azmah; Wirman, Adia Putra
Kieraha Medical Journal Vol 6, No 2 (2024): KIERAHA MEDICAL JOURNAL
Publisher : Fakultas Kedokteran, Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/kmj.v6i2.8931

Abstract

Buruknya perilaku personal hygiene berkaitan dengan permasalahan organ reproduksi wanita, ditandai dengan keputihan patologis. Keputihan patologis sangat rentan terjadi pada ibu hamil dikarenakan peningkatan hormon estrogen dapat menyuburkan pertumbuhan mikroorganime merugikan di dalam vagina. Pertumbuhan mikroorganime mengakibatkan kerusakan epitel sehingga pH vagina semakin meningkat, serta didukung kondisi organ reproduksi yang terletak di area tertutup dan berlipat sehingga mudah berkeringat, lembap, dan kotor. Hal ini akan menyebabkan mikroorganisme tumbuh semakin masif. Penelitian ini bertujuan untuk mengetahui prevalensi jenis infeksi genitalia pada ibu hamil sebagai acuan upaya pencegahan dan penanggulangannya. Penelitian ini menggunakan teknik random sampling dengan jumlah sampel 100 orang ibu hamil terinfeksi genitalia. Penentuan infeksi genitalia didasarkan hasil rekam medis pasien. Hasil penelitian menunjukkan prevalensi paling tinggi terjadinya infeksi genitalia pada ibu hamil disebabkan oleh Vaginosis Bakterialis (VB) sebesar 54%, koinfeksi VB dan Candida albicans 30%, Candida albicans 11%, dan Trichomonas vaginalis 5%. Adanya data prevalensi ini, kami berharap unit kesehatan terkait dapat melakukan upaya preventif sehingga menekan angka kejadian infeksi genitalia pada ibu hamil dan melaksanakan pemeriksaan skrining secara rutin.
EDUKASI PENCEGAHAN DAN DETEKSI DINI KANKER SERVIKS PADA ANGGOTA PMN (PERMODALAN MEKAR MADANI) Purbasari, Euis; Erikardo, Oktadio; Wirman, Adia Putra; Nikmatullah, Nurul Azmah; Sunaryo, Hadi; Riyanti, Hurip Budi
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 1 (2025): MAJU : Indonesian Journal of Community Empowerment, Januari 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/1zwfa578

Abstract

Cervical cancer is a malignant tumor that forms in the cervix. The primary cause of cervical cancer is an infection with oncogenic types of Human Papillomavirus (HPV), particularly types 16 and 18, which lead to abnormal growth in the cervical epithelial tissue. Other risk factors that increase the incidence of cervical cancer include sexual activity with multiple partners, sexually transmitted infections, immune disorders, having many children, low socioeconomic status, and lack of knowledge about reproductive health. The aim of this activity is to enhance participants' knowledge about reproductive organ health as well as the prevention and early detection of cervical cancer. Increasing knowledge can be achieved by providing education on the prevention and early detection of cervical cancer, as well as educating about reproductive health. The results of the educational program showed an increase in knowledge, as reflected in the improved post-test scores.
Co-Authors Adia Putra Wirman Adia Putra Wirman, Adia Putra Aggasy, Savira Yustinah Aldi Pratama Alma Tiara Rahayu Aprilia, Nisa DIAN LESTARI Etin Diah Permanasari Euis Purbasari Hadi Sunaryo Hurip Budi Riyanti M. Wahyu Setiawan Ma&#039;rifatussolihat Ma&#039;rifatussolihat Masyhuda, Irmawati Meri Suzana Nurjanah, Desi Oktadio Erikardo Purbasari, Euis Rahmadilla, Chintya Rahmadini, Nuriza Riyanti, Hurip Budi Samiratul Mufidah Selvi Marcellia Wijiastuti Wijiastuti