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Journal : JSME MIPA UNIMA

Isolasi dan Identifikasi Bakteri Asam Laktat dari Fermentasi Anggur Buah Pala (Myristica fragrans Houtt) Sebagai Penghasil Eksopolisakarida Lawalata, Helen Joane; Rompas, Christny Ferdina; Kansile, Elma Fridayanti
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 1 (2020): JANUARI-FEBRUARI
Publisher : Universitas Negeri Manado

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. Nutmeg fruit is widely used in the medicine formula and also various kinds of processed foods and beverages. Nutmeg meat can be processed into syrup, fruit juice, jam, dodol pala and wine. The fruit is believed to be one of the natural habitats of bacteria, including lactic acid bacteria (BAL). The study aims to determine the type of BALES isolated in nutmeg grapes (Myristica fragrans Houtt) which has the ability to produce exopolysaccharides. Based on the results of the study obtained 15 BAL isolates namely AP1, AP2, AP3, AP4, AP5, AP6, AP7, AP8, AP9, AP10, AP11, AP12, AP13, AP14, and AP15. The exopolysaccharides produced by the isolates are 400 mg/L, 530 mg/L, 1200 mg/L, 730 mg/L, 60 mg/L, 460 mg/L, 600 mg/L, 200 mg/L, 930 mg/L, 1130 mg/L, 200 mg/L, 130 mg/L, 1000 mg/L, 800 mg/L. The next 15 isolates are identified using the profile matching method. The results of the identification show that 15 BAL isolates were included in the genus Lactobacillus and are a species of Lactobacillus bulgaricus.    Keyword : Isolation, Identification, nugmeg grapes (myristica fragrans houtt), lactic acid bacteria, exopolysaccharides
ANALISIS KANDUNGAN BAKTERI ESCHERICHIA COLI PADA AIR SUMUR GALI DI KECAMATAN LIRUNG KABUPATEN KEPULAUAN TALAUD Lawalata, Helen Joan; Tengker, Anita C. Ch.; Maradesa, Sartika
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 2 (2020): MARET - APRIL
Publisher : Universitas Negeri Manado

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ABSTRACT. Research has been carried out to determine the content of the bacterium escherichia coli in well water dug in the sub-district of Lirung, regency of the Talaud Islands. Water is one of the sources of living needs of living things, humans, animals and plants. Well water is a means of clean water from the soil for daily use, such as drinking water, cooking, washing and bathing. Therefore, the water must be pollution-free and meet the quality requirements for clean water. e.coli bacteria are bacteria that generally live in the intestines of humans and animals. This research uses a quantitative descriptive method. The results showed that 9 samples of water wells taken from 3 sites in the Lirung sub-district, in the regency of the Talaud Islands, contained e.coli bacteria which exceeded the maximum threshold not in accordance with the regulations of the Ministry of Health RI No.  416 / MENKES / PER / IX / 1990   Keywords: Bacteria Escherichia coli, Dug Well Water.