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All Journal Dinamisia: Jurnal Pengabdian Kepada Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Katalisator Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat ARRUS Journal of Engineering and Technology Innovative: Journal Of Social Science Research Jurnal Katalisator
Suryani Suryani
Universitas Perintis Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Electrical & Electronics Engineering Engineering Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Public Health Social Sciences Other

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KARAKTERISASI BAKTERIOSIN PADA BAKTERI ASAM LAKTAT Lactobacillus paracasei DARI VIRGIN COCONUT OIL Fauzan Fauzan; Suryani Suryani; Marganof Marganof; Yuliesi Purnawati
Jurnal Katalisator Vol 5, No 1 (2020): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22216/jk.v5i1.5300

Abstract

Virgin Coconut Oil (VCO), so far known for its many uses due to its high lauric acid content. But apparently not only that but the presence of lactic acid bacteria that contain bacteriocin. Where bacteriocin is a peptide that has the ability to inhibit the growth of bacteria and fungi that are pathogenic, can even kill the virus. The purpose of this study was to characterize the bacteriocin present in the Lactobacillus paracasei lactic acid bacteria found in the VCO oil layer. Characterization was carried out in 4 types, namely the ability to grow LAB on the medium carried out by varying a certain pH; The ability of bacteriocin is produced in a medium with a certain pH variation, then analyzes the antimicrobials; The resistance of the bacteriocin to heating with certain temperature variations; Determination of Molecular Weight with SDS-PAGE. The best partial purification results are precipitation with 40% and 60% ammonium sulfate. For the ability to grow LAB, it was able to grow at pH 2-9, the ability of bacteriocin microbial activity at pH 2-6 for E Coli, pH 2-9 for B Sbtillis and pH 2-10 for the test bacteria Listeria monocytogenes and S aureus. This bacteriocin is resistant to 121 0C heating. So it can be concluded that this bacteriocin can live in humansVirgin Coconut Oil (VCO), selama  ini  dikenal  banyak sekali kegunaan nya disebabkan  kandungan asam lauratnya yang tinggi. Tetapi ternyata tidak hanya itu melainkan  adanya bakteri asam laktat yang mengandung bakteriosin. Dimana bakteriosin adalah peptide yang mempunyai kemampuan menghambat pertumbuhan bakteri dan jamur yang berisifat patogen, bahkan dapat membunuh virus. Tujuan penelitian ini adalah untuk mengkarakterisasi bakteriosin yang ada pada bakteri asam laktat  Lactobacillus paracasei yang terdapat pada lapisan minyak VCO.Adapun penelitian ini dimulai dengan mengisolasi bakteriosin menggunakan media MRSB (Merck), pemurnian bakteriosin nya dengan purifikasi parsial  menggunakan  metoda pengendapan  Ammium Sulfat. Karakterisasi dilakukan 4 macam, yaitu Kemampuan tumbuhnya  BAL pada medium dilakukan dengan memvariasikan pH tertentu; Kemampuan bakteriosin dihasilkan pada medium dengan variasi pH tertentu, kemudian menganalisa antimikrobanya; Ketahanan bakteriosin tersebut pada pemanasan dengan variasi temperatur tertentu; Penentuan Berat Molekul dengan SDS-PAGE. Hasil purifikasi parsial yang paling bagus adalah pada pengendapan dengan 40% dan 60% ammonium sulfat . Untuk kemampuan tumbuh BAL, ternyata mampu tumbuh pada pH 2-9, Kemampuan aktivitas mikroba bakteriosin pada pH 2-6 untuk E Coli, pH 2-9 untuk B Sbtillis dan pH 2-10 untuk bakteri uji Listeria monocytogenes dan S aureus.  Bakteriosin ini tahan sampai pemanasan 121 0C. Sehingga dapat disimpulkan bakteriosin ini bisa  hidup pada manusia
Efek Konsentrasi Alkali Pada Virgin Coconut Oil Dalam Proses Pembuatan Sabun Mandi Suryani Suryani; Ellyta Sari; Amelia Amelia
Jurnal Katalisator Vol 3, No 1 (2018): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.611 KB) | DOI: 10.22216/jk.v3i1.3402

Abstract

Telah dilakukan penelitian efek konsentrasi KOH terhadap sabun mandi  menggunakan bahan dasar Virgin Coconut Oil. Sabun mandi ini  merupakan pengembangan produk dari Virgin Coconut Oil (VCO). Sabun dibuat dari reaksi minyak dan larutan alkali dalam skala laboratorium melalui proses dingin menggunakan KOH sebagai larutan alkalinya. Konsentrasi KOH yang digunakan adalah 30% b/v, 40% b/v, 50% b/v, 60% b/v dan 70% b/v sebagai variabel bebas dan sebagai variabel tetapnya adalah waktu pengadukan pada es  selama 30 menit dengan temperatur yang digunakan 280C. Mutu sabun dianalisa dengan menentukan asam lemak bebas, alkali bebas, lemak tak tersabunkan, kadar air,  dan minyak pelikan. Dari penelitian ini ternyata semakin besar konsentrasi KOH yang digunakan menghasilkan sabun yang semakin keras tekstur nya. Mutu sabun yang dihasilkan yang sesuai dengan SNI untuk alkali bebas adalah pada penambahan KOH 405 b/, alkali bebasnya 0,13 % sesuai dengan SNI maksimum 0,14%, asam lemak bebas pada penambahan KOH 50% 6,5 menurut SNI 2,5- 7,5,lemak tak tersabunkan pada penambahan KOH 40% yaitu2,42 menurut SNI maksimum 2,5, minyak pelikan pada penambahan KOH 50%  negatif sesuai dengan SNI tidak boleh ada minyak pelikan. Dapat disimpulkan bahwa penambahan konsentrasi KOH yang menghasilkan sabun mandi bagus dan sesuai dengan SNI adalah antara 40% dan 50%.The effect of KOH concentration on soap has been done using the basic ingredients of Virgin Coconut Oil. This soap is a product development of Virgin Coconut Oil (VCO). Soaps are made from oil reactions and alkaline solutions on a laboratory scale through a cold process using KOH as the alkaline solution. The KOH concentration used was 30% w / v, 40% w / v, 50% w / v, 60% w / v and 70% w / v as the independent variable and as the fixed variable was ice agitation time for 30 min with temperature used 28 0C. The quality of soap is analyzed by determining free fatty acids, free alkali, unabsorbed fat, moisture content, and pelicans oil. From this study it turns out that the greater the concentration of KOH used to produce soap that the harder the texture. The quality of the resulting soap in accordance with the SNI for the free alkali is in the addition of KOH 40% b /v 0.13% free alkali in accordance with the maximum SNI of 0.14%, free fatty acid at the addition of 50% KOH 6.5 according to SNI 2.5 - 7.5, unabsorbed fat at 40% KOH addition 2.42 according to the maximum SNI of 2.5, the pelicans oil at the addition of 50% negative KOH in accordance with the SNI shall not have pelicans oil. It can be concluded that the addition of KOH concentration resulting in good soap and in accordance with SNI is between 40% and 50% 
ANALISIS KALIUM PADA BUAH SEMANGKA (Citrullus lanatus (Thunb.) DENGAN SPEKTROFOTOMETER SERAPAN ATOM Tuty Taslim; suryani suryani; Shinta Fardani; Reny Salim
Jurnal Katalisator Vol 5, No 2 (2020): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22216/jk.v5i2.5717

Abstract

Abstract Kalium adalah salah satu mineral makro yang diperlukan tubuh dalam mengatur keseimbangan muatan elektrolit cairan tubuh. Gangguan terhadap keseimbangan elektrolit akan berpengaruh terhadap metabolisme tubuh. Salah satu sumber Kalium yang paling mudah didapat adalah dari buah semangka Citrullus lanatus (Thunb.) Matsum & Nakai yang sangat banyak ditemukan di daerah Sumatera Barat. Kebiasaan masyarakat untuk menikmati manisnya buah semangka adalah dengan memakan bagian merah daging buah, sedangkan bagian putih di bawah kulit (perikarp) yang berasa tawar cenderung dibuang bersama kulitnya yang menurut studi Agricultural Research Services, mengandung lebih banyak Kalium dan citrulline. Beberapa penelitian telah dilakukan untuk memanfaatkan perikarp buah semangka dan limbah kulit semangka sebagai pembuatan nata, selai dan minuman. Juga penelitian efek kulit semangka pada jumlah neutrofil pada radangakibat luka gores yang diperlakukan pada mencit jantan, tetapi analisis kandungan antara perikarp dan daging buah belum dilakukan. Berdasarkan hal demikian, peneliti mencoba untuk melakukan analisis kadar kalium dalam perikarp (kulit putih) dan daging buah semangka merah (Citrullus lanatus (Thunb.) Matsum & Nakai) secara spektrofotometri serapan atom dari buah semangka yang tumbuh di daerah Kambang Utara, Kecamatan Lengayang, Kabupaten Pesisir Selatan dengan menggunakan spektrofotometri serapan atom. Hasil penelitian menunjukkan bahwa kadar kalium perikarp lebih tinggi yaitu 286,47mg/100g dibanding daging buah 92,76 mg/100g. Hasil uji statistik menggunakan Independent Sample T-Test, dengan sig < 0,025 terdapat perbedaan bermakna kadar kalium pada perikarp dan daging buah semangka.  Abstract Potassium is one of the macro minerals needed by the body in regulating the body's electrolyte charge. Disruption of electrolyte balance will affect the body's metabolism. One of the easiest sources of potassium is from the watermelon fruit Citrullus lanatus (Thunb.) Matsum & Nakai which found in West Sumatra. The habit of people to enjoy the sweetness of watermelon fruit is by eating the red part of the flesh of the fruit, while the white part under the skin (pericarp) which tasteless tends to be removed with the skin which, according to Agricultural Research Services studies, contains more potassium and citrulline. Several studies have been carried out to utilize watermelon pericarp and watermelon rind waste as food and drink production, as well as the effect of watermelon skin on the number of neutrophils in scar inflammation of male mice, but analysis of the different content between pericarp and pulp has not been carried out. Based on this case an analysis of potassium is carried out using atomic absorption spectrophotometry from watermelon that grows at Kambang Utara area, Lengayang District, Pesisir Selatan. The results showed that the level of potassium pericarp was higher at 286,47 mg / 100g compared to 92,76 mg / 100g fruit flesh. Statistical test results using Independent Sample T-Test, with sig <0,025 there is a significant difference in potassium levels in pericarp and watermelon flesh.
IDENTIFIKASI MOLEKULAR BAKTERI ASAM LAKTAT Lactobacillus paracasei YANG ADA PADA LAPISAN MINYAK VCO Suryani Suryani; Dedi Nofiandi; Husni Mukhtar; Melona Siska; Abdi Dharma; Nasril Nasir
Jurnal Katalisator Vol 2, No 2 (2017): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22216/jk.v2i2.2517

Abstract

Virgin Coconut Oil is an oil of coconut milk fermentation that has many uses such as can prevent HIV, because it functions as antibacterial, antifungal and antiviral. Antibacterial, antifungal and antiviral agents are found in bacteria lactic acid bacteriocin, a peptide that can destroy bacterial cells and pathogenic fungi and viral cells. The aim of this study was to identify molecularly lactic acid bacteria isolated and morphologically identified and biochemical tests, from fermented coconut milk. Apparently lactic acid bacteria is Lactobacillus paracasei strain 1.7. Virgin Coconut Oil adalah minyak dari fermentasi santan kelapa yang mempunyai banyak sekali kegunaan diantaranya  dapat mencegah HIV, karena berfungsi sebagai antibakteri, antijamur dan antivirus. Zat antibakteri, antijamur dan antivirus itu terdapat pada bakteri asam laktat yaitu bakteriosin, berupa peptida yang dapat menghancurkan sel bakteri dan jamur patogen serta sel virus. Tujuan penelitian ini adalah mengidentifikasi secara molekular bakteri asam laktat  yang telah diisolasi dan diidentifikasi secara morfologi dan uji – uji biokimia, dari santan yang difermentasi.  Ternyata bakteri asam laktat nya adalah Laktobacillus paracasei strain 1.7. 
Aktivitas Antioksidan Si Ungu Mentawai Reny Salim; Suryani Suryani
Jurnal Katalisator Vol 5, No 1 (2020): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22216/jk.v5i1.5275

Abstract

Research has been conducted on the antioxidant activity of Mentawai leaves in the form of infusion. The result is the strength of the antioxidant activity of purple leaf infusion is moderate. The results obtained give the idea to continue further research based on the concept of growth factor growth research contributing to the secondary metabolites of a plant. Purple leaves contain anthocyanin composition which is seen from the color of the leaves. Anthocyanin compounds are a class of flavonoids that are well-known as one of the secondary metabolites that have antioxidant properties. In this study, the purple leaves sampled were purple leaves and for the stability of the anthocyanin, the pollination process was carried out at high but short temperatures. After that it is extracted using the kinetic maceration method. Maceration uses 70% ethanol solvent in a small amount of acid to keep anthocyanin compounds stable. After that continued with the fractionation process using chloroform, ethyl acetate, and air solvents. The antioxidant strength testing of extracts and fractions is carried out using DPPH as a strong free radical because the testing process is easy and simple. The results of the study obtained IC50 values of extracts and fractions (chloroform, ethyl acetate, air) respectively (9; 138.56; 15.62; 16.65) μg / mL. The category of antioxidant strength from extracts and fractions is strong and moderate.Sebelumnya telah dilakukan penelitian tentang kekuatan aktivitas antioksidan daun ungu Mentawai dalam bentuk infusa. Hasilnya kekuatan aktivitas antioksidan infusa daun ungu tergolong sedang. Hasil yang diperoleh memberikan suatu ide untuk melanjutkan pengujian berikutnya berdasarkan konsep penelitian bahwa faktor lingkungan tumbuh dapat memberikan pengaruh terhadap kandungan metabolit sekunder dari suatu tumbuhan. Daun ungu mengandung senyawa antosianin yang terlihat dari warna daun. Senyawa antosianin merupakan golongan flavonoid yang terkenal sebagai salah satu golongan metabolit sekunder berkhasiat antioksidan. Dalam penelitian ini daun ungu yang dijadikan sampel adalah daun yang seluruhnya berwarna ungu dan untuk menjaga kestabilan antosianinnya maka proses penyerbukan dilakukan dengan suhu tinggi namun waktu pendek. Setelah itu diekstraksi dengan menggunakan metode maserasi kinetik. Maserasi menggunakan pelarut etanol 70% dalam suasana sedikit asam agar senyawa antosianin tetap stabil. Setelah itu dilanjutkan dengan proses fraksinasi menggunakan pelarut kloroform, etil asetat, dan air. Pengujian kekuatan antioksidan ekstrak dan fraksi dilakukan dengan menggunakan zat DPPH sebagai radikal bebas yang kuat namun mudah dan sederhana proses pengujiannya. Hasil dari penelitian diperoleh nilai IC50 ekstrak dan fraksi (kloroform, etil asetat, air) berturut-turut adalah (9; 138,56; 15,62; 16,65) µg/mL. Kategori kekuatan antioksidan dari ekstrak dan fraksi adalah kuat dan sedang.
FORMULATION OF SUNGKAI LEAF ETHANOL EXTRACT POWDER (Peronema canescens Jack.) AND ITS ANTIOXIDANT TEST Sandra Tri Juli Fendri; Farida Rahim; Ririn Khairani; Siska Ferilda; Suryani
JURNAL KATALISATOR Vol. 8 No. 2 (2023): Jurnal Katalisator Volume 8 No. 2, Oktober 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i2.2533

Abstract

Sungkai leaves (Peronema canescens Jack.) is one of the medicinal plants that have antioxidant activity. This study aims to formulate sungkai leaf ethanol extract in the form of instant powder as a health supplement and test the antioxidant activity of   instant   powder   preparations.   Making   dry extract of sungkai leaves   using freeze dryer and antioxidant activity test using DPPH method at a maximum absorption wavelength of 520 nm. The results of instant powder formulation on organoleptis examination instant powder is in the form of fine powder, white with green grains, distinctive smell and slightly sweet taste while the brewed instant powder is in the form of a green liquid, distinctive smell and slightly sweet taste, good flow properties, flow speed 6.63 g / s, resting angle  29.45o,  soluble  time  110  seconds,  water insoluble part 1.528%, moisture content 5.12%, ash content 0.29% and pH 5.59. From all examinations have met the requirements of good preparations. IC values of50 ethanol extract and instant powder were 73.76 ppm and 662.69 ppm respectively. So it can be concluded that sungkai leaf ethanol extract can be formulated as instant powder and sungkai leaf ethanol extract has strong antioxidant activity, while sungkai leaf instant powder has very weak antioxidant activity.
TUBERCULOSIS: PREVENTION THROUGH INCREASING PUBLIC KNOWLEDGE WITH HEALTH PROGRAM COMMUNICATION EDUCATION Rinda Lestari; Suryani Suryani; Yustitia Akbar; Nova Mustika; Marisa Marisa; Ghufran Aulia; BA Martinus
JURNAL KATALISATOR Vol. 8 No. 2 (2023): Jurnal Katalisator Volume 8 No. 2, Oktober 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i2.2626

Abstract

Tuberculosis (TB) is one of the infectious diseases that is still a global health problem. According to World Health Organization (WHO) data in 2020, there are about 10 million new cases of tuberculosis worldwide. TB is still the leading cause of death from infectious diseases in the world, even surpassing the death rate from COVID-19 at the time. In 2019, there were 5,403 TB cases in West Sumatra, in 2019 there were 5,190 TB cases in men (62.70%). Padang is the city with the highest number of TB cases in West Sumatra, with 2,358 cases in 2018, In May 2022, there were 1,500 confirmed TB patients in Padang. Factors contributing to the increase in TB cases in Indonesia, including West Sumatra, are genetics, lifestyle, and level of knowledge. There has been a lot of discussion about tuberculosis, but not many have studied health program communication education about public knowledge about tuberculosis. The purpose of this study was to study health program communication education about public knowledge about tuberculosis. The survey method was used to collect data from respondents representing the community in Padang City. Surveys through written questionnaires and interviews via Google form sent online. Questions in the survey covered aspects such as public knowledge about tuberculosis before and after Promkes education, attitudes towards TB prevention, and factors influencing participation in education programs. The data were analyzed statistically, measuring changes in knowledge before and after Promkes education, and analyzing the factors that influenced the results. Health Program Education Group: Average knowledge about tuberculosis prevention: 8.5 Standard deviation of knowledge: 1.2. Uneducated Group: Average knowledge about tuberculosis prevention: 6.2 Standard deviation of knowledge: 1.5. From the results of the analysis, it can be seen that the group that received Promkes education had a higher average knowledge about tuberculosis prevention compared to the group that did not get education. This difference shows that Promkes education has the potential to have a positive impact on public knowledge about tuberculosis prevention.
Co-Authors Abdi Dharma aisyatul daniati Amelia Amelia Aulia Heniken Putri B.A Martinus BA Martinus Betti Rosita Dedi Nofiandi Desriana Desriana Dyna Puteri Mayaserly Ellyta Sari Farida Rahim Fauzan Fauzan Feby Handayani Femi Earnestly Fendri, Sandra Tri Juli Firdaus Firman Hidayat Firman Syahid Ghufran Aulia Hariadi Hariadi Hasnah Wita Helga Yermadona Hernawati Hernawati Husni Mukhtar Marganof Marganof Marisa Marisa Melona Siska Milantara, Noril Mudinillah, Adam Muhammad Rizky Pratama Nasril Nasir Nova Mustika, Nova Rahmawati Rahmawati Rahmawati Rahmawati Raimon Raimon Reny Salim Ria Afrianti Rinda Lestari Ririn Khairani Rizalman Rizalman Rudi Kurniawan Salim, Reny Sariani, Sariani Sevindrajuta Sevindrajuta Shinta Fardani Sindy Gemaeka Putri Siska Ferilda Tuty Taslim Wardi, Epi Supri Yuliesi Purnawati Yuliesi Purnawati Yuliesi Purnawati Yuliesi Purnawati Yumarni Yumarni Yunita Sabri Yusnaweti Amir YUSRA YUSRA Yustitia Akbar Yustitia Akbar Yustitia Akbar