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Analisis In Silico : Aktivitas Antiinflamasi dan Prediksi Toksisitas Senyawa Metabolit Sekunder Daun Ciplukan (Physalis peruviana L.) Fadzillah, Sania Nur; Hernawati, Diana; Putra, Rinaldi Rizal
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9592

Abstract

The way of life back to nature, including in the field of health, forms the habit of using plants as traditional medicine. Traditional medicine is indicated to be able to cure a variety of diseases. As with the leaves of Ciplukan (Physalis peruviana L.) by the people of Desa Wanasuka Kecamatan Pangalengan Kabupaten Bandung is considered to be used as a traditional medicine for inflammatory diseases such as rheumatism, asthma, and gastritis (maag) due to side effects and toxicity produced relatively small when compared with modern medicine. The aim of this study is to identify the potential of the secondary metabolite compound found in the leaves of the Ciplulan (Physalis peruviana L.) as a candidate for anti-inflammatory drugs. These research methods are molecular methods of docking through physical chemical properties testing, pharmacokinetic profiles of test compounds through site Pre-ADMET (Absorption, distribution, metabolism, excretion, and toxicity) and Lipinski Rule of Five. The results of this study showed that the binding affinity of caffeic acid compounds with PTGS2 receptors (PDB ID: 5F19) showed a more negative result compared to the comparative drugs Aspirin and Ibuprofen -7.4. There was a molecular interaction similar to Aspirin, in which the addition of amino acid residues from ASN 382 and TYR 385 produces a strong irreversible inhibition of PTGS2 (PDB ID: 5F19) and is predicted to inhibit prostaglandin H synthase and indicate that caffeic acid is capable of producing irreverible inactivation of PTGS2 (PDB ID:5F19). So potentially a candidate for a safer anti inflammatory drug for the body. Because it has almost the same or close potential as Aspirin against inflammatory diseases.
Studi Etnobotani Tumbuhan Familia Araceae Di Desa Mertajaya, Kecamatan Bojongasih, Tasikmalaya Fauziyah, Naila Nurul; Hernawati, Diana; Putra, Rinaldi Rizal
Bioma : Jurnal Biologi dan Pembelajaran Biologi Vol. 10 No. 2 (2025): BIOMA: JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v10i2.4242

Abstract

Masyarakat Desa Mertajaya memanfaatkan tumbuhan dari familia Araceae Tujuan penelitian ini untuk mendeskripsikan studi etnobotani tentang tumbuhan familia Araceae di Desa Mertajaya Kecamatan Bojongasih Kabupaten Tasikmalaya. Penelitian ini dilaksanakan pada tanggal 11 Maret hingga 22 April 2025 dengan menggunakan metode penelitian deskriptif kualitatif. Pengumpulan data dilakukan dengan wawancara semi terstuktur dengan pengambilan sampel melalui teknik snowball sampling, observasi, serta dokumentasi. Berdasarkan hasil wawancara dengan 12 informan, didapatkan 9 spesies yang termasuk kedalam 8 genus tumbuhan familia Araceae, yaitu Alocasia macrorrhizos, Amorphophallus paeoniifolius, Caladium Bicolor, Colocasia esculenta, Dieffenbachia seguine, Homalomena rubescenss, Syngonium auritum, Syngonium podophyllum, dan Xanthosoma sagittifolium. Tumbuhan tersebut dimanfaatkan sebagai sumber pangan, obat tradisional, tanaman hias, pakan ternak, peralatan tradisional, hingga tradisi budaya.
Digital plant project in biology education: Bridging sociodemographic gaps in plant awareness Fitriani, Rita; Hernawati, Diana; Putra, Rinaldi Rizal
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 2 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.60879

Abstract

Plant awareness disparity poses a persistent challenge in biology education, limiting recognition, appreciation, and engagement with plants among preservice biology teachers (PSTs). This study investigated the influence of sociodemographic factors on PSTs’ plant awareness gains and evaluated the effectiveness of a digital plant project in addressing these disparities. A total of 134 PSTs participated in a project-based intervention involving observation, documentation, and analysis of local plants using digital tools. Plant awareness was measured through the validated Plant Awareness Disparity (PAD) Questionnaire, which covers four indicators: attention, attitude, knowledge, and relative interest. Sociodemographic data, including gender, age, residence, family income, family experience with plants, and plant-based cultural practices, were analyzed using t-tests and one-way ANOVA. The results showed significant improvements across all indicators of plant awareness after the intervention. Gender, residence, family income, family experience, and cultural practices significantly affected awareness gains, while age did not. Female students, urban residents, and participants from higher-income or plant-engaged families achieved higher gains. The digital plant project fostered active, experiential, and technology-supported learning, enabling equitable participation and engagement with botanical content. Overall, the findings highlight the potential of integrating digital and project-based approaches in biology education to enhance both conceptual understanding and affective appreciation of plants, while supporting inclusive curricula that address disparities in plant awareness.
PENGARUH CAHAYA DAN TEMPERATUR TERHADAP PERTUMBUHAN TUNAS DAN PROFIL PROTEIN TANAMAN ANGGREK Phalaenopsis amabilis TRANSGENIK PEMBAWA GEN Ubipro::PaFT Putra, Rinaldi Rizal; Mercuriani, Ixora Sartika; Semiarti, Endang
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2483

Abstract

Penelitian ini bertujuan untuk mencari kondisi yang tepat dalam percepatan pembungaan tanaman Phalaenopsis amabilis transgenik yang telah disisipi gen penentu waktu pembungaan Ubipro::PaFT. Metode penelitian ini menggunakan tanaman transgenik pembawa gen Ubipro::PaFT umur 18 bulan setelah penanaman. Tanaman ditumbuhkan pada inkubator dengan pencahayaan menggunakan lampu LED putih dan kombinasi LED putih biru, dengan fotoperiodisitas 8 jam terang 16 jam gelap, suhu 25ºC pada fase terang dan 20ºC pada fase gelap selama 20 minggu. Setelah 20 minggu pertumbuhan tanaman, dilakukan analisis profil protein dengan metode SDS-PAGE untuk mengetahui protein yang diproduksi pada setiap fase pertumbuhan yang diamati.Hasil penelitian menunjukkan kombinasi cahaya LED putih dan biru meningkatkan pembentukan daun sebesar 60%, panjang daun 70,58%, tetapi belum diperoleh kemunculan infloresen. Analisis profil protein menunjukkan terbentuknya protein dengan ukuran 108,57; 71,30; 56,16; 40,85; 26,79; 13,27; dan 13,12 kilodalton pada tanaman transgenik, tetapi tidak terdeteksi protein dengan ukuran 19,65 kDa yang sesuai dengan berat molekul protein PaFT, sementara protein dengan ukuran sekitar 56,16 kDa sesuai dengan berat molekul protein POH1(Phalaenopsis Orchid Homeobox1). Hal ini menunjukkan bahwa gen vegetatif POH1 mampu menghambat aktivasi gen PaFT pada tanaman P. amabilis transgenik umur 20 minggu, sehingga tanaman masih dalam fase juvenil dan belum mampu diinduksi untuk berbunga.
Analisis E. Coli Pada Air Minum Dalam Kemasan Yang Beredar Di Kota Tasikmalaya Meylani, Vita; Putra, Rinaldi Rizal
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9241

Abstract

Drinking water is the main need of humans as living things. Over time, humans are more practical so that for drinking needs they prefer to use bottled drinking water or refill drinking water. The high demand for bottled water raises the number of drinking water companies that issue bottled drinking water products. However, the quality of bottled water still needs to be assessed because it is not through pasteurization or other processing. So the microbiological content remains to be investigated. This study aimed to determine the bacteriological content in bottled drinking water. This study uses the Most Probable Number (MPN) method to test its bacterial content and gamma ray radiation to test its radiosensitivity. The sample in this study was bottled drinking water of various brands circulating in the City of Tasikmalaya. Based on the research results obtained from 13 samples there is 1 sample containing E. coli which is code B1 with a total bacterial content of 7 cells per 100 ml. So it can be concluded L samples are known to contain E. coli as much as 1.9 x 105 cells per ml.