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Journal : JURNAL AGROINDUSTRI HALAL

PENGARUH PEMBERIAN SERBUK EKSTRAK KELOPAK BUNGA ROSELA (Hibiscus sabdariffa L.) TERHADAP SISTEM IMUN TIKUS SPRAGUE DAWLEY Mardiah Mardiah; Dwi Aryanti Nur’utami; Arti Hastuti
JURNAL AGROINDUSTRI HALAL Vol. 5 No. 1 (2019): Jurnal Agroindustri Halal
Publisher : Lembaga Riset dan Pengabdian Kepada Masyarakat Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.217 KB) | DOI: 10.30997/jah.v5i1.1676

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian serbuk ekstrak kelopak bunga rosela terhadap sistem imun tikus Sprague Dawley. Parameter yang diamati adalah leukosit, differensial leukosit, organ limfoid (limpa dan hati), total serum protein dan serum albumin dari darah tikus Sprague Dawley. Hewan uji dibagi atas 4 kelompok yang masing-masing terdiri dari 5 ekor tikus. Kelompok pertama adalah kelompok normal, kelompok 2 diberikan stimuno forte dosis 1,35 mg/KgBB, kelompok 3 diberikan serbuk ekstrak rosela I dosis 40,5 mg/KgBB, dan kelompok 4 diberikan serbuk ekstrak rosela II dosis 81 mg/KgBB secara sonde selama 28 hari. Pada hari ke-0 dan hari ke-29 dilakukan pengambilan darah. Analisa data menggunakan perhitungan selisih, yang hasilnya dibandingkan dengan nilai normal tikus kondisi sehat. Berdasarkan hasil penelitian pemberin serbuk ekstrak rosela I dosis 40,5 mg/KgBB KgBB dapat mempertahankan fungsi sistem imun pada tikus Sprague Dawley dengan peningkatan nilai rata-rata jumlah neutrofil segmen sebesar 42,2%, total serum protein sebesar 10,99g/dl, bobot limpa relatif sebesar 0,22% dan bobot hati relatif sebesar 3,27% yang masih dalam batas normal hewan coba dalam kondisi sehat sehingga dapat memberikan efek protektif terhadap serangan antigen. Kata kunci :  Serbuk ekstrak,  rosela, sistem imun
Total Phenolic Content and Antioxidant Activity of Pasteurized Milk With the Addition of Centella asiatica Leaf Extract Distya Riski Hapsari; Intan Kusumaningrum; Arti Hastuti; Caesandra Indah Arlina; Lia Amelia
JURNAL AGROINDUSTRI HALAL Vol. 9 No. 3 (2023): Jurnal Agroindustri Halal 9(3)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v9i3.7433

Abstract

Milk has a high nutritional value. Minimal processing that can extend the shelf life of milk is pasteurization, but it can damage the nutritional content of milk. One of the additional efforts to improve milk quality and maintain the nutritional content of milk is to provide additional active compounds that act as antioxidants for milk consumers and the milk product itself. The use of Centella asiatica leaf extract as an ingredient is because there are at least 139 active compounds. This study aimed to study the total phenolic content and antioxidant activity of pasteurized milk with the addition of Centella asiatica leaf extract. The research was carried out in two stages, namely making gotu kola leaf extract and making pasteurized milk by adding gotu kola leaf extract concentrations of 0%, 0.25%, 0.5%, and 0.75%. Product analysis included total phenol analysis using Folin Ciocalteau and antioxidant activity using the DPPH method. Adding Centella asiatica leaf extract can increase total phenolic content and antioxidant activity. The total phenolic content obtained by adding Centella asiatica leaf extract at 0%, 0.25%, 0.50%, and 0.75% were 0.58 mgGAE/g, 1.93 mgGAE/g, respectively 2.33 mgGAE/g, and 3.41 mgGAE/g. At the same time, the results of antioxidant activity were 7.68%, 31.93%, 37.57, and 40.35%, respectively.
Aktivitas Antioksidan dan Karakteristik Kimia Stik Pegagan Riski Hapsari, Distya; Nurhalimah, Siti; Hastuti, Arti; Pratami, Aprilia
JURNAL AGROINDUSTRI HALAL Vol. 10 No. 2 (2024): Jurnal Agroindustri Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v10i2.11160

Abstract

Gotu kola leaves contain active compounds which are beneficial for the human body. Gotu kola leaves can be added to processed foods, including sticks. This research added sticks with a concentration of 10%, 15%, and 20% of gotu kola leaves paste. Then, antioxidants activity, crude fiber content, water content, ash content, protein content, fat content, and carbohydrate content were analyzed. After that, gotu kola sticks were selected. Based on research results, the greater the addition of gotu kola leaves, the greater the antioxidant activity, water content, ash content, protein content, and crude fiber content. Gotu kola sticks were selected with the addition of 15% gotu kola leaves. The results of gotu kola chosen sticks had an antioxidant content of 53671.90 ppm, water content 1.32%, ash content 0.99%, protein content 7.75%, fat content 39.04%, carbohydrate content 50.91% and crude fiber 32.20%.