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All Journal Jurnal Sains dan Teknologi Farmasi Biology, Medicine, & Natural Product Chemistry JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Syntax Literate: Jurnal Ilmiah Indonesia Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ipteks Terapan Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Insight: Jurnal Bimbingan dan Konseling Jurnal Pengabdian Harapan Ibu (JPHI) BioEksakta : Jurnal Ilmiah Biologi Unsoed Khazanah Intelektual Jurnal Ipteks Terapan : research of applied science and education Jurnal Ilmiah Bakti Farmasi pendidikan, science, teknologi, dan ekonomi Jurnal Katalisator Riset Informasi Kesehatan Jurnal Pengabdian Harapan Ibu (JPHI) Kartika : Jurnal Ilmiah Farmasi
Desi Sagita
Universitas Adiwangsa Jambi Jl. Sersan Muslim RT.24 Kebun Kopi Kelurahan Tehok Jambi
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Katalisator

 1 
The KARAKTERISASI ENZIM Β-SIKLODEKSTRIN DARI ISOLAT LOKAL (BTS3A, BTS3-B DAN CK-2) PROVINSI JAMBI Desi Sagita; Rahmadevi
JURNAL KATALISATOR Vol. 6 No. 1 (2021): jurnal Katalisator Volume 6 No 1, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (744.126 KB) | DOI: 10.62769/katalisator.v6i1.215

Abstract

The β-cyclodextrin glucosyltransferase (β-CGTase) enzyme is an extracellular enzyme that converts starch to cyclodextrins. Cyclodextrins are widely used in the pharmaceutical industry, one of which is to increase solubility. This enzyme is produced by bacteria, especially the genus Bacillus sp. BTS3A, BTS3B and CK-2 isolates that produce CGTase enzymes have been isolated from the soil of cassava and potato plantations. The bacteria are fermented in liquid Horikoshi media, then centrifuged at 4 ° C and then concentrated by freeze dry. The enzyme was purified by the ammonium sulfate precipitation method up to 59.61% for BTS3A, 59.6% for BTS3B and 58.1% for CK-2. Then dialyzed using a cellophane membrane with cut off 14 KDa in phosphate buffer. The cyclization activity of crude enzymes was characterized by varying incubation times of 30, 60, 120 minutes and incubation temperatures of 30 ℃, 37 ℃, 55 ℃. CGTase cyclization activity was measured based on the decrease in phenolphthalein absorbance at a wavelength of 550 nm. The β-CGTase enzyme from the three isolates showed the optimum percentage reduction at 55°C for 30 minutes incubation
Co-Authors A, Eva A, Widya Afrudi Rudheka Alfiana, Dessinta Anggresani, Lia Anis Pratiwi Ara Ofiana Armini Hadriyati Astridawati, Indah Barmi Hartesi Dahlia, Dimbi Dedi Eva A Fatimah Ria Sitompul Fhatia Medina Fitri, Kurnia Fiwi Juni Trisia G, Sri Hani WH HARTESI HARTESI HARTESI, HARTESI Hastuti Hastuti Helsa Raudatul Qalbi Hesty Murtiah Ibrahim, Puji Astuti Ichwani, Muhammad Nurul Iis mira anggraini Ilahi, Silvi Riska Imelda br. Siahaan Indah Astridawati Indri Meirista Julia Ambar Sari juliper nainggolan Kurniadi, Helman Lia Anggresani Lia Anggresani Lili Andriani Lili Andriani Lili Andriani Lili Loreza Sitanggang Linuria Linuria Linuria, Linuria Lufita, Lufita M, Putri Maya Gusri Yanti Medina, Fhatia Mery Safitri Michiko Mamesah Miftah RS Muhammad Nurul Ichwani Muhammad Randa Paska Harianty Novita Pratiwi, Anis Putri M Rahmadevi Randa, Muhammad Ratna Delfira Retty Filliani Rizky Ramdani Fajri Rizky Yulion Rozalti Rozalti RS, Miftah Rudheka, Afrudi Safitri, Mery Santi Perawati Santi Perawati Santi Perawati, Santi Sarah Christin Sari, Julia Ambar Septa Pratama Siti Hamidatul 'Aliyah Sri G Susanti Susanti Sutrisno , Deny Triana Wulansari Wahyu Perdaka WH, Hani Widya A Yuli Fitri Utami Yuliawati Yuliawati Yuni Andriani ‘Aliyah, Siti Hamidatul