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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Biologi Udayana Biotropika BIOMA : Jurnal Biologi Makassar BIOEDUSCIENCE Menara Perkebunan Agro Bali: Agricultural Journal Jurnal Riset Biologi dan Aplikasinya JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Biosaintifika: Journal of Biology & Biology Education
Turhadi Turhadi
(Scopus ID: 57207793599), Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.10

Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress Turhadi TURHADI; Hayati MINARSIH; Imron RIYADI; . PRIYONO; Asmini BUDIANI
Menara Perkebunan Vol. 88 No. 2 (2020): 88 (2), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i2.386

Abstract

Drought is one of the limiting factors in crop cultivation, such as in oil palm (Elaeis guineensis Jacq.). The transgenic approaches are expected to increase plant tolerance to drought stress and minimize low productivity when drought occurs. Proline is an osmoprotectant compound in plants which its biosynthesis involved the P5CS gene. The objective of this study was to evaluate the tolerance level of P5CS-transgenic oil palm to drought stress induced by polyethylene glycol 6000 (PEG-6000). In this present study, the transgenic and non-transgenic oil palms were treated by  0, 2, and 4% PEG-6000 under in vitro conditions. The experiment was arranged as a factorial completely randomized design with three replications. The drought level score, total chlorophyll content, carotenoids, and proline content, as well as P5CS gene expression in leaf tissues were observed at 7 and 14 days after stress treatments. The result showed that transgenic plantlets had a lower drought level score than those of non-transgenic lines. A concentration of 4% PEG-6000 treatment reduced the total chlorophyll and carotenoids contents than that of 2% concentration in non-transgenic plantlets at 7 and 14 day after treatments (DAT). In addition, proline content and P5CS gene expression level in transgenic had been significantly increased during stress treatment. Based on these results, it can be concluded that the P5CS transgene increased the drought stress tolerance of oil palm.
Transformation of DHN1 gene and DHN promoter constructs into sugarcane calli, regeneration of the calli, and acclimatization of the plantlets Hayati Minarsih; Fauziatul Fitriyah; Annisa Aulya Aksa; Turhadi Turhadi; Deden Sukmadjaya; Sustiprajitno Sustiprajitno
Menara Perkebunan Vol. 91 No. 1 (2023): 91 (1), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i1.512

Abstract

Dehydrin is known to have an important role in plant response and adaptation to abiotic stresses including drought and high salinity. Previous research reported the isolation of the full-length coding sequence (CDS) of DHN1 from sugarcane var. PSJT 941, and it shares a high homology with DHN genes from sorghum and other sugarcane varieties. In this study, the full-length CDS was cloned under the constitutive CaMV35S promoter and transformed into sugarcane calli mediated by Agrobacterium tumefaciens. The DHN promoter, Pr-1DHNSo, was also successfully isolated from the sugarcane var. PSJT 941 and cloned into the pBI121 expression vector. The promoter construct was subsequently transformed into sugarcane calli of var. Kidang Kencana. Transgenic sugarcane carrying DHN1 gene and DHN promoter constructs were regenerated according to the standard protocol of sugarcane tissue culture. Optimization of an acclimatization protocol using modified post-rooting media was also conducted and the resulting protocol reduced the total mortality rates of the transformed plantlets. The presence of the gene and promoter constructs was periodically tested by PCR using specific primers. The genotyping results showed that the constructs were present for more than a year after transformation.
Characterization and morphological development of oil palm transformed-callus on modified culture media Yuli Setiawati; Imron Riyadi; Dini Astika Sari; Rizka Tamania Saptari; Masna Maya Sinta; Hayati Minarsih; Turhadi; Riza Arief Putranto
Menara Perkebunan Vol. 92 No. 2 (2024): 92(2), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i2.576

Abstract

Genome editing through cisgenesis develops into scientific breakthroughs in accelerating oil palm breeding programs. However, one remaining problem is the low success of transformed-calli regeneration, while its scientific explanation is still underexplored. This study aimed to characterize and regenerate transformed-calli using various amino acids and antioxidants.  Transformed callus that did not regenerate (un-regenerated transformed callus or UTC) after the transformation process was taken, then T-DNA integration was detected using the NPTII gene. Furthermore, the UTC was divided into four types based on morphological characteristics. The four types of UTCs were regenerated on media enriched with glutamine (for Type-1 callus), cysteine and putrescine (for Type-2 callus), and a combination of cysteine and ascorbic acid (for Type-3 and Type-4 callus). The research results obtained NPTII successfully amplified with a band size of 700bp. The results showed that on Type-1 callus, enrichment media with 10 mg L-1 L-glutamine could induce the formation of new nodular structures on UTC Type-1. On Type-2, media enriched with 5 mg L-1 L-cysteine + 20 mg L-1 putrescine increased the density of callus structures. Media enriched with 25 mg L-1 ascorbic acid + 25 mg L-1 L-cysteine could prevent the spread of brown callus on Type-3 callus, while Type-4 callus did not show any response and became dry. Our new findings will facilitate the basic research and unregenerated transformed callus and morphological callus development behavior in oil palm.
Inhibitory Effect of Aqueous Garlic (Allium sativum) Bulb Extract on Growth and Physiological Response Dynamics of Two Palm Species Setiyanto, Afif Eka Rahma; Nashafi, Azizuddin Muhammad; Turhadi, Turhadi; Siswanto, Dian
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22108

Abstract

The Merrill palm (Adonidia merrillii) and the Yellow palm (Dypsis lutescens) are hosts of various pests. Garlic (Allium sativum) phytochemicals are applied as a phytopesticide and affect the plant host as well. On the other hand, studies related to garlic phytochemicals' effect on these palms are limited.  The present study was conducted to explore the effect of aqueous garlic bulb extract (AGBE) on the Merrill palm and the Yellow palm on those physiological dynamics and growth responses to apply the best concentration as a phytopesticide and observe the palm performance. AGBE is conducted by maceration and application by spraying. Variation AGBE concentrations were 12.5 g/100 mL, 25 g/100 mL, and 50 g/100 mL. The results revealed the profiles of the two palms were different, causing significant differences in growth, in which the Merrill palm was taller while Yellow palm greater leaf number increase; physiological response of two palms was not differing significantly on carbon dioxide concentration but differ significantly on stomatal opening percentage in 28 DAS (day after spraying) and chlorophyll content in 56 DAS; AGBE 25 g/100 mL suppress height increase significantly but leaf number increase not affected; AGBE did not influenced significantly on carbon dioxide, AGBE 50 g/100 mL significantly influenced on stomatal opening in 42 and 56 DAS but chlorophyll not significantly different, overall AGBE tend to inhibit all physiological response. This study concludes that AGBE concentrations in the current study are not recommended as a phytopesticide on both palms due to all concentrations generally inhibiting the growth and physiological response of these palms.
OPTIMASI SUHU ANNEALING MARKA MOLEKULER SIMPLE SEQUENCE REPEAT (SSR) UNTUK STUDI KERAGAMAN GENETIK Aglaonema pictum (Roxb.) Kunth Maharani, Fira Shabrina Bintang; Hariri, Muhammad Rifqi; Turhadi, Turhadi
JURNAL BIOSENSE Vol 8 No 4 (2025): Edisi Oktober 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i4.5695

Abstract

Aglaonema pictum (Roxb.) Kunth merupakan tanaman hias populer di dunia karena memiliki variasi corak warna daun. Studi tentang keragaman genetik A. pictum masih jarang dilakukan, sehingga upaya ini perlu dilakukan. Salah satu penanda molekuler yang dapat digunakan untuk mengetahui keragaman genetik yaitu Simple Sequence Repeats (SSR). Tujuan penelitian ini adalah mengidentifikasi kondisi optimum pada primer SSR untuk studi keragaman genetik A. pictum. Metode yang digunakan adalah pengumpulan sampel, ekstraksi DNA menggunakan cetyltrimethylammonium bromide (CTAB), amplifikasi DNA menggunakan primer SSR (OSR13, RM10, RM6470, OSR14, MRG2172_RT2172, MRG2153_RGP2153, RM249, RM100, MRG2881_RT2881, dan RM7240) dengan menggunakan Polymerase Chain Reaction (PCR) pada kisaran suhu 52-54℃, 55-59℃, 47.2-50.9℃, dan 47.6-50.4℃, elektroforesis dan dokumentasi gel agarosa, serta analisis data. Hasil penelitian menunjukkan bahwa dua dari sembilan primer SSR (RM249 dan RM7240) dapat diamplifikasi dengan baik pada suhu 49,3 ˚C dan 52,6 ˚C. Kedua suhu tersebut mengindikasikan suhu optimum yang dapat mengamplifikasi DNA sampel A. pictum. Sementara itu, primer OSR13, RM10, RM6470, OSR14, MRG2172_RT2172, MRG2153_RGP2153, RM100, MRG2881_RT2881 tidak berhasil teramplifikasi dengan baik dan spesifik pada rentang suhu annealing yang digunakan dalam penelitian ini.
Pemetaan Spesies Asing Invasif Hydrocotyle leucocephala Cham. & Schltdl. di Kebun Raya Bogor Solihah, Fifi Mar’atun; Hariri, Muhammad Rifqi; Turhadi, Turhadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10841

Abstract

The presence of undocumented alien flora threatens Indonesia’s flora richness and diversity due to unpredictable invasion risks. Hydrocotyle leucocephala is an alien flora that has been reported as invasive in Sri Lanka and Nigeria and has distributed to Indonesia. Therefore, mapping the distribution and describe the morphological of Hydrocotyle leucocephala is necessary to detect the initial introduction or further spread of this species. The data collection was carried out by exploring the Bogor Botanical Gardens. Environmental factors are measured and documented where Hydrocotyle leucocephala occurs. For observation, Hydrocotyle leucocephala was taken and their morphology described. As a results, Hydrocotyle leucocephala was spread over vak V.L, V.G, V.M, IX.D, VIII.B and parking lot near the conservation building in the Bogor Botanical Gardens. It is also known that Hydrocotyle leucocephala is a creeping herbaceous plant with glabrous stems, kidney-shaped leaves, white flowers without petals and green fruit with persistent styles.
Distribusi Jenis Asing Invasif Melothria pendula L. (Cucurbitaceae) di Kawasan Konservasi Ilmiah Kebun Raya Bogor Kekung, Cristina Mega Meyfiani; Husaini, Iin Pertiwi A; Turhadi, Turhadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12369

Abstract

Melothria pendula is belong to Cucurbitaceae family and originates from America continent. Previous research reported findings of M. pendula in several regions in Indonesia, such as in Bogor Botanical Gardens (KRB) as invasive species. The appearance of M. pendula in KRB has not yet been inventoried. As invasive species, M. pendula has the potential to be a threat to collection and native plants. This research aims to report the distribution of M. pendula in the scientific conservation area of KRB and measure the abiotic factors in the locations where M. pendula was found. This research was exploratory method which was carried out by exploring and morphological characterization of M. pendula. Measurement of abiotic factors, including temperature, air humidity, light intensity, and wind speed at each site where M. pendula was found. QGIS is used to mapping the distribution of M. pendula. Based on this study showed that there were 40 individuals of M. pendula which found distributed randomly in 15 sites (VG, VD, VB, XXIVA, IIM, IIC, IIP, XVI.VII, IVF, XXIVB, IIO, XVF, XXIIA, IIK, and seedling area) in KRB. M. pendula habitats in KRB characterized by forest land cover type with temperature of 31.1 ± 0.96 ℃, air humidity of 69 ± 2.73%, light intensity 3183.9 ± 3095 lux, and wind speed 0.7 ± 0.51 m/s.
Enhancing Community Knowledge and Skills in Organic Farming and Natural Dyes for Ikat Weaving in Ensaid Panjang, Sintang, West Kalimantan Leksono, Amin Setyo; Mustafa, Irfan; Yanuwiadi, Bagyo; Turhadi, Turhadi; Afandhi, Aminudin; Zairina, Anisa
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 5 No 2 (2025)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2025.005.02.118

Abstract

This community service program aimed to analyze the level of knowledge and identify strategies for introducing organic farming and natural dye production technologies for ikat weaving among the Dayak community in Ensaid Panjang Village, Sintang, West Kalimantan. The program employed an action research method with a problem-based learning approach. The activities began with a community needs assessment, followed by the delivery of materials and information through lectures, simulations, demonstrations, and training sessions. A total of 52 participants took part in the program. Among them, several informants were selected based on their knowledge and expertise. The key informants included the head of Ensaid Panjang Village, the Betang customary elders, and representatives from the Ensaid Panjang community. The results showed that three leading indicators demonstrated significant improvement in participants’ understanding. These included enhanced community skills in producing natural dyes from local plants for ikat weaving, the ability to process organic fertilizers from household waste, garbage, and animal manure, as well as the strengthening of institutional roles that support the production and marketing of woven fabrics. The substantial increase in understanding indicated that this program successfully improved participants’ knowledge and skills. Overall, the average increase in community understanding and achievement was 11%, with a notable 51% increase in knowledge of composting specifically.
SUHU ANNEALING PRIMER INTER SIMPLE SEQUENCE REPEAT (ISSR) PADA DNA Aglaonema pictum (Roxb.) Kunth Baharuddin, Muh. Fikry; Hariri, Muhammad Rifqi; Turhadi, Turhadi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 11 No. 1 (2026): Bioma : Januari - Juni 2026
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v11i1.45426

Abstract

Aglaonema pictum is one of the most popular ornamental plants in the community. However, studies on the genetic diversity of A. pictum are still limited. Based on this, it is necessary to study the genetic diversity of A. pictum, one of which uses DNA-based markers such as Inter Simple Sequence Repeats (ISSR). In order to study of genetic diversity of A. pictum effectively and efficiently, one of the efforts that can be done is optimizing the annealing temperature for ISSR primers to be used. The purpose of this study was to optimize the effective Inter Simple Sequence Repeats (ISSR) primers on DNA of A. pictum. The methods used include DNA extraction using cetyltrimethylammonium bromide (CTAB), DNA amplification using primers ISSR1, ISSR2, ISSR3, ISSR4, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12) using Polymerase Chain Reaction (PCR) at temperature ranges of 52-54 ˚C. The results showed that ten out of 12 ISSR primers could amplify the DNA of A. pictum using optimal annealing temperatures. ISSR primers which identified optimum annealing temperature were ISSR1, ISSR3, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12. Primer of ISSR1 and ISSR11 showed optimum annealing temperature at 52,6°C. Primer of ISSR3, ISSR8, and ISSR9 showed optimum annealing temperature at 53,6°C, while primer of ISSR6, ISSR7, and ISSR10 showed optimum annealing temperature at 54°C. in other hands, primer of ISSR2 nad ISSR4 did not yet identified their optimum annealing in all tested temperature range. Kata kunci : Aglaonema pictum, Annealing Temperature, Genetic Diversity, Inter Simple Sequence Repeats
Co-Authors . Hamim . Miftahudin . PRIYONO Abdullah Abdullah Aliarani Rosyidiana Putri Amalia Danti Safa Audia Amin Setyo Leksono Aminudin Afandhi Anisa Zairina Annisa Aulya Aksa Asmini Budiani Bagyo Yanuwiadi Baharuddin, Muh. Fikry Bedjo Bedjo Briliyan Natalina Sudarjayanti Cristina Mega Meyfiani Kekung Dawama Nur Fadlilah Deden Sukmadjaya Dian Siswanto Dini Astika Sari Eko Suyanto Else Vilia Setiawan Elvina Rashida Khairi Estri Laras Arumingtyas Ezra, Achmad fatchiyah . Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fathiyah Nurul Izzah Fatma Yona, Hafidza Fauziatul Fitriyah Fifi Mar’atun Solihah Fifi Mar’atun Solihah Firda Nuri Asyhari Galuh Arkana Giral Baines Mora Grace Christy Haikal Ageng Maulana Hamim Hamim Hamim Hamim Hayati Minarsih Hayati Minarsih Hayati Minarsih Hose, Victor Alvianoes Guterez Husaini, Iin Pertiwi A Iin Pertiwi A Husaini Imron RIYADI Imron Riyadi Irfan Mustafa Karuniasari, Nadaa Kekung, Cristina Mega Meyfiani Khairunnisa Hidaya, Amira Luchman Hakim Maharani, Fira Shabrina Bintang Masna Maya Sinta Mentari Putri Pratami Miftahuddin Miftahuddin Miftahuddin Miftahudin . Mufidah Afiyanti Muhammad Rifqi Hariri Muhammad Rifqi Hariri Muhammad Wisam Wira Sakti Munif Ghulamahdi Muwaffiq Faza, Ahmad Nabila Shafa Yumna Salsabila Nandagesta Aurelia Shafa Wagmi Nashafi, Azizuddin Muhammad Nuraini Rosyadah Padmana, I Made Dhena Radya Pratama, Ardo Cahya Rahmadini, Agnia Fadillah Rahmi Izzati Raissa Kendra Ainiyah Rayhan Yugo Nurul Maulana Riza Arief Putranto Rizka Tamania Saptari Rodiyati Azrianingsih Rodiyati Azrianingsih Salma Wahid Marseti Septhyanti Aprilia Kavitarna Serafinah Indriyani Serafinah Indriyani Setiyanto, Afif Eka Rahma Shafala Safa, Muhammad Solihah, Fifi Mar’atun Suharjono Suharjono Sustiprajitno Sustiprajitno Talitha Pangestu, Twistka Yohanna Sisilia Grace Sirait Yuli Setiawati