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All Journal Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Biologi Udayana Biotropika BIOMA : Jurnal Biologi Makassar BIOEDUSCIENCE Indonesian Journal of Chemistry Menara Perkebunan Agro Bali: Agricultural Journal Jurnal Riset Biologi dan Aplikasinya JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) BIO-CONS: Jurnal Biologi dan Konservasi Biosaintifika: Journal of Biology & Biology Education
Turhadi Turhadi
(Scopus ID: 57207793599), Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Comparison of γ-Oryzanol Content Using HPLC Profiling and Bioactivity in Three Indonesian Brown Rice (Oryza sativa L.) Varieties Umar, Ja’far; Palis, Christine Natalia; Suyanto, Eko; Turhadi, Turhadi; Wihastuti, Titin Andri; Fatchiyah, Fatchiyah
Indonesian Journal of Chemistry Vol 26, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.100139

Abstract

Brown rice (BrR) is a nutrient-rich staple food and a potential source of γ-oryzanol, which has the potential to promote health effects in hypercholesterolemia. However, the availability and biological mechanism of γ-oryzanol in Indonesian BrR remain unexplored. This study aimed to characterize γ-oryzanol content in three Indonesian BrR varieties, Black Madras, Lawang, and UB BrR, and evaluate its biological functions for hypercholesterolemia. γ-Oryzanol was identified using HPLC, antioxidant activity by the ferric reducing antioxidant power (FRAP) method, and anticholesterol activity by the Lieberman-Burchard method. In silico analysis was performed to assess γ-oryzanol derivatives' interaction with microsomal triglyceride transfer protein (MTP). Black Madras BrR showed the highest γ-oryzanol content (0.3007 ± 0.0011 g). γ-Oryzanol was comprised of four main derivatives: cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, campesteryl ferulate, and β-sitosteryl ferulate. Black Madras BrR exhibited strong antioxidant (IC50 18.89 ± 0.37 μg/mL) and anticholesterol (IC50 14.02 ± 0.23 µg/mL) activities. Docking simulations revealed that γ-oryzanol derivatives interact with MTP lipid-binding residues (Leu643, Ile666, Phe813, Val817) with the same binding energy as lomitapide. Molecular dynamics indicated γ-oryzanol stable interaction, closer to lomitapide. These findings proposed the potential bioactivity of γ-oryzanol from Black Madras BrR as antioxidant, anticholesterol, and a natural MTP inhibitor for hypercholesterolemia treatment.
Hubungan Fenetik antara Citrus hystrix Dc., Citrus X Microcarpa Bunge, dan Citrus Sp. Koleksi Kebun Raya Bogor Berdasarkan Karakter Morfologi dan Anatomi Kurniawan, Jessica Amadea; Mulyani, Melza; Turhadi, Turhadi
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 2 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i2.2483

Abstract

Marga Citrus (jeruk) merupakan salah satu marga yang anggotanya beragam yang terdiri dari 255 jenis dengan 44% diantaranya termasuk kelompok Mandarin (Mandarins group). Hubungan kekerabatan secara fenetik antar jenis di dalam marga Citrus sp. dapat diamati menggunakan karakter morfologi maupun anatomi. Tujuan dari penelitian ini adalah mengetahui hubungan fenetik antara jeruk purut (C. hystrix), jeruk kasturi (C. microcarpa), dan Citrus sp. menggunakan karakter morfologi dan anatomi. Pada penelitian ini digunakan sampel jeruk purut, jeruk kasturi, dan Citrus sp. koleksi Kebun Raya Bogor (KRB). Karakter organ vegetatif (batang dan daun) serta organ generatif (bunga, buah dan biji) dilakukan pada ketiga spesimen. Data yang diperoleh selanjutnya dianalisis secara fenetik menggunakan program komputer PAST v4.03. Hasil penelitian menunjukkan bahwa Citrus  sp. dan C. hystrix memiliki tingkat kemiripan yang lebih tinggi antar keduanya jika dibandingkan dengan C. microcarpa. Beberapa karakter yang menjadi pembeda utama antara C. microcarpa, C. hystrix, dan Citrus sp. koleksi KRB adalah ujung dan pangkal daun, warna kulit buah, ukuran buah, bentuk biji, dan kerapatan stomata.  
DISTRIBUSI KONYAL (Passiflora suberosa L.) DI KEBUN RAYA BOGOR, JAWA BARAT Althoriq, Luthfi; Hariri, Muhammad Rifqi; Turhadi, Turhadi
Biospecies Vol. 19 No. 1 (2026): Januari 2026
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i1.46650

Abstract

Konyal (Passiflora suberosa L.) is a wild and invasive plant belonging to the Passifloraceae. The existence of P. suberosa as an invasive species can threaten the existence of local plants. As a scientific conservation area, the Kebun Raya Bogor (KRB) is a habitat for Konyal. One effort that can be made to minimize the threat of invasive species is through mapping. The aim of this research was to map the distribution of konyal (P. suberosa) in the scientific conservation area of Kebun Raya Bogor and measure environmental factors in the habitat of konyal (P. suberosa). The research was carried out using an exploratory method throughout the Kebun Raya Bogor area in January 2024. Documentation of komyal (P. suberosa) and measurements of environmental factors in its growing habitat were carried out during exploring activities. In addition, distribution coordinate data was recorded and used for mapping using QGIS v3.36.2 program. The research results showed that the konyal  (P. suberosa) in the KRB was spread over six areas, including II A, II C, II D, II F, III I, and  XIII A. Environmental factors at the location where P. suberosa was found in the Kebur Raya Bogor area, such as air humidity (70 – 77%), temperature (26 – 33 ˚C), and light intensity (2250 – 16200 lux). Distribution of konyal (P. suberosa) in KRB area showed high density in vak II (II A, II C, II D, and II F). The location vak II has vegetation with sparse canopy and low shade, thereby increasing the sunlight intensity compared to other areas.
KARAKTERISASI MORFOLOGI DAN OPTIMASI PRIMER INTER SIMPLE SEQUENCE REPEATS (ISSR) UNTUK STUDI AWAL KERAGAMAN GENETIK Syzygium zeylanicum (L.) Faza, Ahmad Muwaffiq; Martiansyah, Irfan; Turhadi, Turhadi
Biospecies Vol. 19 No. 1 (2026): Januari 2026
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i1.48204

Abstract

Syzygium zeylanicum (Myrtaceae) is a species that grows in the Indo-Malayan region. Different growing environments for S. zeylanicum can influence morphological characteristics and reflect genetic diversity. The objective of this study was to describe the morphological characteristics of S. zeylanicum and optimize Inter Simple Sequence Repeats (ISSR) primers as an initial step toward studying the genetic diversity of S. zeylanicum. This study was conducted by collecting S. zeylanicum samples from the Bogor Botanical Garden at six locations: V.B.84, V.B.84a, II.Q.87, II.Q.87a, V.C.54a, and V.C.97a. Leaf and branch samples were collected for morphological characterization. DNA isolation was performed using 2% CTAB. Optimization was performed for 12 ISSR primers (ISSR1, ISSR2, ISSR3, ISSR4, ISSR5, ISSR6, ISSR7, ISSR8, ISSR9, ISSR10, ISSR11, and ISSR12) with annealing temperature ranges of 52 – 54 °C, 52.1 – 5 3.1 °C, 55 – 58 °C, and 45 – 50 °C. Characterization showed that S. zeylanicum leaves are simple, lanceolate, caudate tips, aequilateral bases, entire margins, brochidodromous venation, opposite leaf arrangement, petiolate attachment, abaxially glabrous, and adaxially lustrous. The leaf length, leaf width, and branch diameter of S. zeylanicum ranged from 6 – 8.7 cm, 2.3 – 3.03 cm, and 1.45 – 1.96 cm, respectively. In addition, 11 of the 12 ISSR primers successfully obtained optimal annealing temperatures, including ISSR1 (57.7 °C), ISSR2 (52.8 °C), ISSR3 (56.6 °C), ISSR5 (52.7 °C), ISSR6 (52.9 °C), ISSR7 (47.8 °C), ISSR8 (47.8 °C), ISSR9 (52.6 °C), ISSR10 (53.0 °C), ISSR11 (49.7 °C), and ISSR12 (49.0 °C). Only ISSR4 primer did not produce DNA bands after optimization at all temperature ranges used.
Co-Authors . Hamim . Miftahudin . PRIYONO Abdullah Abdullah Aliarani Rosyidiana Putri Althoriq, Luthfi Amalia Danti Safa Audia Amin Setyo Leksono Aminudin Afandhi Anisa Zairina Annisa Aulya Aksa Asmini Budiani Bagyo Yanuwiadi Baharuddin, Muh. Fikry Bedjo Bedjo Briliyan Natalina Sudarjayanti Cristina Mega Meyfiani Kekung Dawama Nur Fadlilah Deden Sukmadjaya Dian Siswanto Dini Astika Sari Eko Suyanto Else Vilia Setiawan Elvina Rashida Khairi Estri Laras Arumingtyas Ezra, Achmad fatchiyah . Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fathiyah Nurul Izzah Fatma Yona, Hafidza Fauziatul Fitriyah Faza, Ahmad Muwaffiq Fifi Mar’atun Solihah Fifi Mar’atun Solihah Firda Nuri Asyhari Galuh Arkana Giral Baines Mora Grace Christy Haikal Ageng Maulana Hamim Hamim Hamim Hamim Hayati Minarsih Hayati Minarsih Hayati Minarsih Hose, Victor Alvianoes Guterez Husaini, Iin Pertiwi A Iin Pertiwi A Husaini Imron RIYADI Imron Riyadi Irfan Mustafa Karuniasari, Nadaa Kekung, Cristina Mega Meyfiani Khairunnisa Hidaya, Amira Kurniawan, Jessica Amadea Luchman Hakim Maharani, Fira Shabrina Bintang MARTIANSYAH, Irfan Masna Maya Sinta Mentari Putri Pratami Miftahuddin Miftahuddin Miftahuddin Miftahudin . Mufidah Afiyanti Muhammad Rifqi Hariri Muhammad Rifqi Hariri Muhammad Wisam Wira Sakti Mulyani, Melza Munif Ghulamahdi Muwaffiq Faza, Ahmad Nabila Shafa Yumna Salsabila Nandagesta Aurelia Shafa Wagmi Nashafi, Azizuddin Muhammad Nuraini Rosyadah Padmana, I Made Dhena Radya Palis, Christine Natalia Pratama, Ardo Cahya Rahmadini, Agnia Fadillah Rahmi Izzati Raissa Kendra Ainiyah Rayhan Yugo Nurul Maulana Riza Arief Putranto Rizka Tamania Saptari Rodiyati Azrianingsih Rodiyati Azrianingsih Salma Wahid Marseti Septhyanti Aprilia Kavitarna Serafinah Indriyani Serafinah Indriyani Setiyanto, Afif Eka Rahma Shafala Safa, Muhammad Solihah, Fifi Mar’atun Suharjono Suharjono Sustiprajitno Sustiprajitno Talitha Pangestu, Twistka Titin Andri Wihastuti Umar, Ja’far Yohanna Sisilia Grace Sirait Yuli Setiawati