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All Journal International Journal of Electrical and Computer Engineering ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Mutiara Medika: Jurnal Kedokteran dan Kesehatan BERDIKARI : Jurnal Inovasi dan Penerapan Ipteks SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Jurnal Kreativitas PKM PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Prosiding Seminar Nasional Program Pengabdian Masyarakat Denta: Jurnal Kedokteran Gigi Lux Mensana: Journal of Scientific Health Odonto dental journal
Sofiani, Erma
Universitas Muhammadiyah Yogyakarta
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Metabolism-independent phenomenon in ethanolic propolis inhibitory capacity towards enterococcus spp proteolytic activity Adiningrat, Arya; Prabowo, Rizqi Alifna Waskita; Kurnia, Ridho; Septianti, Nurul Fitri Fika; Maulana, Ikhsan; Sofiani, Erma
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.206-214

Abstract

Background: Root canal bacteria produce many virulence factors which are responsible for endodontic pathological states. Bacteria are assumed to utilize energy from bacterial cell metabolism activity for producing these virulence factors. Propolis extracts are commonly reported to have antibacterial abilities against dental pathogens. The purpose of this study is to investigate the possible correlation between bacterial proteolytic and metabolism activities under the treatment of ethanolic extract of propolis (EEP).Method: The 0.00125%; 0.05%; 0.1%; 0.2%; 0.4%; and 0.8% ethanolic propolis were prepared for recovery rate confirmative procedure, proteolytic, and metabolism activity assay, with 2% of chlorhexidine gluconate (CHX) was used as a positive control. The bacteria were cultured in brain heart infusion (BHI) media after EEP treatment. Bacterial suspension was initially prepared in broth culture dilution with BHI media, followed by the gelatin liquefaction measurement for proteolytic assay. Phenol-red and arginine dehydrogenase enriched media for observing both carbohydrate and arginine metabolism activities, respectively, in the clinical Enterococcus spp. and E. faecalis ATCC 29212.Result: The recovery rate of the bacteria was not terminated after several EEP treatments. Proteolytic activity of the bacteria was likely decreased in several EEP treatments. EEP tended to affect the carbohydrate and arginine metabolism of the bacteria in certain fashions.Conclusion: This study suggested that the EEP treatment affected both proteolytic and metabolism activity in negative regulation tendencies.
Co-Authors Adiningrat, Arya Annisa Ramadanti Ardian Mareta, Dhita Astri Bilqis Azizah Azura Azza Tsabita Cahya Damarjati Dwi Suhartiningtyas Dwi Suhartiningtyas, Dwi Dwi Suhartinintyas Eldurr, Salma Nabita Erisona, Dita Sri Fara Amrina Revada Fauri Hakim Fauzia Nilam Orienty Indri Kurniasih Jihan Nadhirah Natasya Kumalasari, Mutia Kunsputri, Fahma Aldihyah Kurnia, Ridho Laila Ma’rifatul Azizah Martadewi , Frida Arba Maulana, Ikhsan Maulida Nurhasanah Nurhasanah, Maulida Prabowo, Rizqi Alifna Waskita Putri, Saskia Sabrina Rahayu, Dwi Novita Regia Aristiyanto Rizqylayli Fajriyani Rovi, Fineza Sa’idah, Haliyati Sartika Puspita Saskia Sabrina Putri Septianti, Nurul Fitri Fika Setyawan, Erwin Slamet Riyadi Tata Pandu Ibadah Z Vina Khairunnisa Kenji