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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA International Journal of Applied Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Geografi, Edukasi dan Lingkungan ANNALES BOGORIENSES Jurnal Agrotek Tropika Journal of Tropical Crop Science Warta Penelitian Perhubungan Menara Perkebunan JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Tanaman Tembakau, Serat & Minyak Industri Perbal : Jurnal Pertanian Berkelanjutan Warta Penelitian Perhubungan Annales Bogorienses
Efendi, Darda
Agronomy And Horticulture Department, Faculty Of Agriculture, Bogor Agricultural University Jl. Meranti, IPB Darmaga Campus, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Transportation Veterinary

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Journal : ANNALES BOGORIENSES

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Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto, Rudiyanto; Efendi, Darda; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (723.325 KB) | DOI: 10.1234/91

Abstract

Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.
Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto Rudiyanto; Darda Efendi; Tri Muji Ermayanti
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (723.325 KB) | DOI: 10.14203/ann.bogor.2014.v18.n1.35-43

Abstract

Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.
Co-Authors , Dorly . Angela Ade Wachjar Ade Wachjar Alfia Annur Aini Azizi ANDRIA AGUSTA Andria Agusta Angela, . Anneke Pesik Asti Kusriyanti Azizi, Alfia Annur Aini Azizi, Alfia Annur Aini Bambang S PURWOKO Bambang S. Purwoko Bambang S. Purwoko Bhaskara, Sandhi Yoga C Hanny Wijaya Cece Suhara Dacholfany, Imanullah Dede Robiatul Adawiyah Deden Derajat Matra Deden Sukmadjaja Dewi Sukma Dianto, Fajar Didy Sopandie DINARTY, DINY Diny Dinarti Djoko Santoso Djoko Santoso Don R LaBonte Dwi Utami Nur Usmani E. Gunawan Edi Santosa Endang Gunawan Entit Hermawan Eny Widajati Erlin Vira Novianti Erwin Al-Hafiizh Evan Maulana Fajar Dianto Fajarudin, A Farida, Naimatul Fitri Fatma Wardani Fitri Fatma Wardani Furqoni, Hafith Gunawan, E. Gustaaf A Wattimena Halimah Widyaningrum Hanifah Muthmainnah Haryanti, Dyra heliyana hermawati Ika Mariska Ika Roostika IKA ROOSTIKA Ika Roostika Ika Roostika Ika Roostika Ika Roostika Tambunan Imanullah Dacholfany Imron Riyadi Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iswari S Dewi Joko Ridho Witono Kasutjianingati . Katerin Ninariyani Ketty Suketi Kusriyanti, Asti Laela Sari laela Sari, laela Lisnandar, Dea Silvia Lolliani Martin, Andri MASKROMO, ISMAIL Maulana, Evan Maulana, Mohamad Akhbar Maya Melati Mayasari Yamin, Mayasari Megayani Sri Rahayu Mohamad Akhbar Maulana Mutiara Utami Ni Made Wasundhari Dharma Suarka Ninariyani, Katerin Nindita, Anggi NOVARIANTO, HENGKY Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Ogie Satriadi Purwito, Agus Purwito Putra, Mirza R Rahayu, Resa Sri Rahmat Budiarto Rahmi Fajri RARA PUSPITA DEWI LIMA WATI RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Reflinur Reflinur Resa Sri Rahayu Riry Prihatini Roedhy Poerwanto Rofiq, Muhamad Abdul Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto S Noorrohmah Satriadi, Ogie Sedyo Harsono SEDYO HARTONO Slamet Susanto Sobir Sobir SOEKISMAN TJITROSEMITO Solly Aryza Sony Hartono Wijaya SRI HENDRASTUTI HIDAYAT Sri Yuliani Sri Yuliani Sudarsono Sulassih, . Surya Diantina Surya Kurnia Putra, Dicky Susetio, Muhammad Tambunan, Ika Roostika Tambunan, Ika Roostika Tanari, Yulinda Taruna Shafa Arzam, Taruna Shafa TENDA, ELSJE T. Tiara, Dede TRI ASMIRA DAMAYANTI Tri Istianingsih Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Triokoesoemaningtyas Triokoesoemaningtyas Vandra Kurniawan Wahyu Fikrinda Wattimena, and Gustaaf Adolf Wida W. Khumaero Willy Bayuardi Suwarno Winarso D. Widodo Witono, dan Joko Ridho Yande Artha Gautama Yosi Zendra Joni Yosi Zendra Joni Yundari, Yundari