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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA International Journal of Applied Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Geografi, Edukasi dan Lingkungan ANNALES BOGORIENSES Jurnal Agrotek Tropika Journal of Tropical Crop Science Warta Penelitian Perhubungan Menara Perkebunan JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Tanaman Tembakau, Serat & Minyak Industri Perbal : Jurnal Pertanian Berkelanjutan Warta Penelitian Perhubungan Annales Bogorienses
Efendi, Darda
Agronomy And Horticulture Department, Faculty Of Agriculture, Bogor Agricultural University Jl. Meranti, IPB Darmaga Campus, Bogor 16680, Indonesia
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Journal : JURNAL BIOLOGI INDONESIA

 1 
PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

Abstract

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots.  Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation  
PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

Abstract

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots.  Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation  
Co-Authors , Dorly . Angela Ade Wachjar Ade Wachjar Alfia Annur Aini Azizi ANDRIA AGUSTA Andria Agusta Angela, . Anneke Pesik Asti Kusriyanti Azizi, Alfia Annur Aini Azizi, Alfia Annur Aini Bambang S PURWOKO Bambang S. Purwoko Bambang S. Purwoko Bhaskara, Sandhi Yoga C Hanny Wijaya Cece Suhara Dacholfany, Imanullah Dede Robiatul Adawiyah Deden Derajat Matra Deden Sukmadjaja Dewi Sukma Dianto, Fajar Didy Sopandie DINARTY, DINY Diny Dinarti Djoko Santoso Djoko Santoso Don R LaBonte Dwi Utami Nur Usmani E. Gunawan Edi Santosa Endang Gunawan Entit Hermawan Eny Widajati Erlin Vira Novianti Erwin Al-Hafiizh Evan Maulana Fajar Dianto Fajarudin, A Farida, Naimatul Fitri Fatma Wardani Fitri Fatma Wardani Furqoni, Hafith Gunawan, E. Gustaaf A Wattimena Halimah Widyaningrum Hanifah Muthmainnah Haryanti, Dyra heliyana hermawati Ika Mariska Ika Roostika Ika Roostika Ika Roostika Ika Roostika IKA ROOSTIKA Ika Roostika Tambunan Imanullah Dacholfany Imron Riyadi Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iswari S Dewi Joko Ridho Witono Kasutjianingati . Katerin Ninariyani Ketty Suketi Kusriyanti, Asti Laela Sari laela Sari, laela Lisnandar, Dea Silvia Lolliani Martin, Andri MASKROMO, ISMAIL Maulana, Evan Maulana, Mohamad Akhbar Maya Melati Mayasari Yamin, Mayasari Megayani Sri Rahayu Mohamad Akhbar Maulana Mutiara Utami Ni Made Wasundhari Dharma Suarka Ninariyani, Katerin Nindita, Anggi NOVARIANTO, HENGKY Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Ogie Satriadi Purwito, Agus Purwito Putra, Mirza R Rahayu, Resa Sri Rahmat Budiarto Rahmi Fajri RARA PUSPITA DEWI LIMA WATI RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Reflinur Reflinur Resa Sri Rahayu Riry Prihatini Roedhy Poerwanto Rofiq, Muhamad Abdul Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto S Noorrohmah Satriadi, Ogie Sedyo Harsono SEDYO HARTONO Slamet Susanto Sobir Sobir SOEKISMAN TJITROSEMITO Solly Aryza Sony Hartono Wijaya SRI HENDRASTUTI HIDAYAT Sri Yuliani Sri Yuliani Sudarsono Sulassih, . Surya Diantina Surya Kurnia Putra, Dicky Susetio, Muhammad Tambunan, Ika Roostika Tambunan, Ika Roostika Tanari, Yulinda Taruna Shafa Arzam, Taruna Shafa TENDA, ELSJE T. Tiara, Dede TRI ASMIRA DAMAYANTI Tri Istianingsih Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Triokoesoemaningtyas Triokoesoemaningtyas Vandra Kurniawan Wahyu Fikrinda Wattimena, and Gustaaf Adolf Wida W. Khumaero Willy Bayuardi Suwarno Winarso D. Widodo Witono, dan Joko Ridho Yande Artha Gautama Yosi Zendra Joni Yosi Zendra Joni Yundari, Yundari