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All Journal EDUSAINS AKSIOMA: Jurnal Program Studi Pendidikan Matematika Phenomenon : Jurnal Pendidikan MIPA Proceeding of the Electrical Engineering Computer Science and Informatics Science and Technology Indonesia JRPM (Jurnal Review Pembelajaran Matematika) Jurnal Matematika: MANTIK Jurnal Penelitian Pendidikan IPA (JPPIPA) MUST: Journal of Mathematics Education, Science and Technology Jurnal Tadris Matematika Jurnal Medik Veteriner Alifmatika: Jurnal Pendidikan dan Pembelajaran Matematika BERNAS: Jurnal Pengabdian Kepada Masyarakat Pedagogy : Jurnal Pendidikan Matematika Kharisma: Jurnal Administrasi dan Manajemen Pendidikan Engagement: Jurnal Pengabdian Kepada Masyarakat Jurnal Tadris Matematika SOCIAL EDU: Jurnal Ilmu Sosial dan Pendidikan
Ahmad Lubab
Universitas Islam Negeri Sunan Ampel Surabaya
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Characterization and Immunogenicity of Thyroid Stimulating Hormone (TSH) Isolated from Neonatal Umbilical Cord Serum Almas Dwi Khairana; Saidun Fiddaroini; Devi Ramadani; Muhammad Fikri Nur; Andreas Budi Wijaya; Akhmad Sabarudin; Anna Safitri; Dyah Kinasih Wuragil; Wibi Riawan; Achmad Rudijanto; Ahmad Lubab; Aulanni'am Aulanni'am
Science and Technology Indonesia Vol. 11 No. 4 (2026): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2026.11.4.1659-1678

Abstract

Congenital hypothyroidism (CH) is among the most prevalent and preventable causes of intellectual disability in neonates worldwide, with thyroid-stimulating hormone (TSH) serving as the primary biochemical screening biomarker. In Indonesia, limited availability of locally produced anti-TSH antibodies restricts the scalability of neonatal CH screening programs. This study aimed to isolate TSH from neonatal umbilical cord serum and to evaluate its capacity to induce polyclonal antibodies suitable for immunoassay-based CH screening. Umbilical cord serum was processed using PBST-PMSF buffer and ethanol precipitation, followed by SDS-PAGE separation. A 28 kDa protein fraction was confirmed as a TSH-enriched antigen by Western blot analysis using a monoclonal anti-TSHβ antibody, then isolated via electroelution for immunogenic assessment. Structural characterization was performed by ATR-FTIR spectroscopy and LC-HRMS amino acid profiling. The electroeluted fraction was subsequently used as an immunogen in New Zealand White rabbits, and antibody performance was evaluated by indirect ELISA, dot blot analysis, splenic histopathology, and immunohistochemistry (IHC). Western blot confirmed a single immunoreactive band at 28 kDa. ATR-FTIR revealed a characteristic absorption at 1046 cm⁻¹, consistent with carbohydrate-associated structures of glycoproteins, while LC-HRMS detected amino acids compatible with TSH subunit composition, including arginine, leucine, lysine, and cystine. Immunization successfully induced polyclonal antibody production, with peak reactivity at a 1:200 serum dilution by indirect ELISA. Dot blot analysis demonstrated optimal antigen-antibody binding at a 1:10 dilution. Splenic histopathology confirmed systemic immune activation, evidenced by significant white pulp enlargement (110.25 ± 11.67 µm vs. 88.47 ± 6.67 µm; P < 0.05). IHC demonstrated specific antibody reactivity localized to thyrotroph cells of the rat anterior pituitary. These findings provide preliminary evidence supporting the utility of neonatal cord serum-derived TSH as an antigenic source for local polyclonal antibody production. Further analytical and clinical validation, including cross-reactivity assessment and diagnostic performance evaluation, is required prior to application in routine CH screening.
ANALISIS KEMAMPUAN REPRESENTASI MATEMATIS SISWA KELAS VII PADA MATERI DIAGRAM BATANG BERDASARKAN TEORI DUVAL Umi Salimah; Yuni Arrifadah; Ahmad Lubab; Heri Susanto
Pedagogy: Jurnal Pendidikan Matematika Vol. 11 No. 4 (2026): Pedagogy : Jurnal Pendidikan Matematika
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/fway8707

Abstract

Representasi matematis berperan penting dalam penyajian dan interpretasi data, tetapi siswa sering kesulitan ketika harus berpindah antarbentuk representasi, misalnya dari tabel ke diagram batang. Penelitian ini bertujuan mendeskripsikan kemampuan representasi matematis siswa kelas VII pada materi diagram batang berdasarkan teori representasi semiotik Duval yang membedakan aktivitas pembentukan, perlakuan (treatment), dan konversi (conversion). Penelitian menggunakan pendekatan kualitatif deskriptif dengan dukungan data kuantitatif sederhana. Subjek adalah 27 siswa kelas VII. Data berupa jawaban tertulis pada lembar kerja mandiri yang memuat lima tugas: menjelaskan langkah membuat diagram batang, membaca diagram dan menghitung selisih, menyajikan data tabel dalam diagram batang, menafsirkan data terbanyak dan tersedikit, serta menyajikan data kontekstual buatan sendiri. Jawaban dikode pada skala 0–2 dan dianalisis secara deskriptif. Rerata capaian sebesar 81,1%; 19 siswa (70,4%) berkategori tinggi, 7 siswa (25,9%) sedang, dan 1 siswa (3,7%) rendah. Capaian tertinggi terdapat pada perlakuan aritmetika setelah membaca diagram dan pembentukan data kontekstual (masing-masing 87,0%), sedangkan terendah pada konversi diagram ke bahasa verbal (74,1%). Kesulitan utama berupa tertukarnya peran sumbu pada tabel yang kedua kolomnya numerik (5 siswa), ketidaktelitian tinggi batang, dan penjelasan verbal yang bersifat umum. Pembelajaran perlu mengoordinasikan register tabel, grafik, dan verbal secara eksplisit.
Co-Authors Abdulloh Hamid Achmad Rudijanto Achmad Rudijanto Aditya Hernowo Aditya Tri Hernowo Adyanti, Deasy Alfiah Agus Prasetyo Kurniawan Agus Prasetyo Kurniawan Ahmad Choirul Anam Ahmad Fauzi Ahmad Hanif Asyhar Ahmad Zaenal Arifin Akhmad Sabarudin Akhmad Sabarudin Ali Mustofa Almas Dwi Khairana Almas Dwi Khairana Alvin Nadiroh Andreas Budi Wijaya Aning Wida Yanti Anisatur Rizqiyah Anna Safitri Arie Srihardyastutie Aris Fanani Aulanni&#039;am Aulanni&#039;am Aulanni&#039;am Aulanni&#039;am Aulanni'am Aulanni'am Aulanni'am Ayunda Nova Millania Cindy Amelia Yulianingrum Deasy Alfiah Adyanti Devi Ramadani Devi Ramadani Dian C. Rini Novitasari Dian Candra Rini Novitasari Dian Yuliati Dodik Prasetyo Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Dyah Kinasih Wuragil Elly Susanti Harini, Sri Heri Susanto Hilman Nurmahdi Husniayatus Salamah Zainiyati ian Candra Rini Novitasari Jo Estu Nugrahenny Khurotul Mufidah Luthfianisa Ramadina Maria Ulfa Maryam Qadarin Moh. Hafiyusholeh Mohammad Nafie Jauhari Muh. Fikry Baharuddin Muhammad Arif Purnomo Muhammad Fikri Nur Muhammad Fikri Nur Nailil Inayah Natasya Adiba Zahrah Natasya Adiba Zahrah Nur Afifah Olly Indrajani Olly Indrajani Putroue Keumala Intan Rini Novitasari, ian Candra Rizqiyah, Anisatur Rulli Rosandi Saidun Fiddaroini Saidun Fiddaroini Salsabila Sri Harini Sri Widyarti Sri Widyarti Susanti, Elly Sutiman Bambang Sumitro Sutiman Bambang Sumitro Syifa Mustika Turmudi, Turmudi Umi Salimah Upik Khoirul Abidin Wibi Riawan Wibi Riawan Wibi Riawan Wibi Riawan Wika Dianita Utami Yuni Arrifadah Yuni Arrifadah Yuniar Farida Zainullah Zuhri