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Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/628595

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 Î¼l, 15 Î¼l, and 10 Î¼l. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 Î¼l is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.

Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/628595

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 Î¼l, 15 Î¼l, and 10 Î¼l. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 Î¼l is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.

BIOSCAFFOLD FROM MOUSE EMBRYONIC FIBROBLAST MAINTAINS THE PLURIPOTENCY OF MOUSE EMBRYONIC STEM CELLS Jannah, Rifqah Mifthahul; Naroeni, Aroem; Novianti, Titta
Journal of Stem Cell Research and Tissue Engineering Vol. 7 No. 1 (2023): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v7i1.38140

Cell culture using a 3D method provides Various cell culture strategies have been developed using synthetic or biological materials; most existing publications use many reagents. Bioscaffold from mouse embryonic fibroblast (MEF) enhances cell attachment, interaction, and production of growth factors. Since bioscaffolds could maintain and stimulate pluripotency of stem cells, we conducted this study to prove bioscaffold function. Bioscaffold was prepared from MEF cultured in DMEM complete medium supplemented with dextran sulfate and L-ascorbic acid to increase extracellular matrix production. This medium acts as an embryo stem cell (ESC) culture medium. We used a Tali-cytometer to identify and quantify stem cells based on Sox2 and Oct4 proteins, markers of stemness. ESC culture using bioscaffold maintained the pluripotency of ESC as indicated by the presence of Oct 4 and Sox2 as ESC markers compared to MEF culture. From this research, the bioscaffold from MEF can be developed as media for ESC to improve propagation. Furthermore, it is a model for tissue engineering and in vitro organ development.

Appropriate Concentration of Curcumin as a Growth Factor in Neural Stem Cells Novianti, Titta; Kurniawan, Muhamad Panji Januarsyah; Nainggolan, Ita M
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20862

The growth of Neural Stem Cell (NSC) in adult organisms is limited. Therefore, growth factors are needed to stimulate NSC cell proliferation and differentiation. Herbal Curcumin may be a growth factor. We promoted the growth of Cryopreserved Rat Cortical NSC cells with Curcumin (0.1 µM; 0.5 µM; 1 µM; 2 µM), DMSO, and synthetic growth factors (bFGF, TGF, and heparin). We analyzed the proliferation ability of NSCs by WST-1 assay, cell morphology, and expression of NCS cell marker genes (Nestin, MAP, and Sox2). Morphological analysis showed that cells reproduced optimally at 0.5 µM. The one-way ANOVA and Tukey's posthoc test on the WST-1 test showed significant differences between 0.5 µMCurcumin and other treatment groups. Sox2, MAP-2, and Nestin gene expression peaked at 0.5 µM. The appropriate concentration of Curcumin to stimulate NSC proliferation is 0.5 µM. Herbal extract curcumin has the same effect as commercial growth factors and can substitute synthetic growth factors. Curcumin acts as a growth factor that stimulates the proliferation of mouse NSCs.

The utilization of Tepache Probiotic Drink for health benefits and creating household-scale economic opportunities Titta Novianti; Seprianto Seprianto; Rini Hidayati
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 9 No. 3 (2024): August 2024
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v9i3.14016

Probiotic drinks are needed to enrich the microbiome in the digestive organs. Increasing age causes a decrease in the ability of cells to metabolize food, resulting in various degenerative diseases. Tepache probiotic drinks, made from pineapple skin, contain magnesium minerals for bone health, vitamins A, B, and C. Through fermentation, it is enriched by lactic acid bacteria which play a role in helping food metabolism in the intestines and increasing the immune response. Counseling on making tepache probiotic drinks for Family Empowerment and Welfare (PKK) mothers RW 11 Pamulang Timur is expected to improve public health and stimulate the household economy. The method of implementing community service is carried out by means of socialization of the benefits of probiotic drinks, training in making tepache probiotic drinks, financial management and marketing training, and production assistance. Tepache drinks are made from pineapple skin fermented for 24 hours using brown sugar, water, cloves, and cinnamon. The success of fermentation is indicated by the presence of white grains on the surface of the liquid. The fermentation results are filtered and can be consumed directly. The results of the counseling produced a tepache drink product that has been routinely consumed by PKK mothers RW 11 Pamulang Timur, and has an impact on reducing knee pain, tension, blood sugar, uric acid, and cholesterol. The results of household-scale sales have not shown significant profits, but simple calculations if pursued will increase household income.

WORKSHOP ISOLASI DNA DAN PENGENALAN ALAT LABORATORIUM BIOTEKNOLOGI BAGI GURU BIOLOGI SMA/MA SE JAKARTA Seprianto Seprianto; Henny Saraswati; Febriana Dwi Wahyuni; Titta Novianti; Adri Nora; Aroem Naroeni; Putri Handayani
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 3: Agustus 2022
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v2i3.2780

Pengembangan bioteknologi dalam pengelolaan sumberdaya hayati dapat menjadi potensi terbesar dalam bidang biodiversitas dan menjadi aset nyata untuk kesejahteraan masyarakat. Bioteknologi didukung dengan metode – metode mutakhir serta fasilitas laboratorium yang memadai untuk mewujudkan keilmuan yang mampu dipahami oleh para siswa dengan transfer ilmu yang baik dari seorang guru biologi. Guru yang menguasai materi pelajaran dengan baik, menggunakan metode dan media pembelajaran yang tepat, mampu mengelola kelas dengan baik akan memberi pengaruh yang positif terhadap prestasi belajar siswa. Pengabdian ini bertujuan untuk menambah wawasan materi tentang DNA, mampu melakukan isolasi DNA dengan benar serta mengenal jenis alat – alat laboratorium yang digunakan untuk mengisolasi dan visualisasi DNA. Peserta dihadiri sebanyak 23 orang yang tersebar dari berbagai SMAN/SMAS se Jakarta. Pelaksanan pengabdian dilakukan dengan 2 metode yaitu klasikal dan praktek di laboratorium. Kegiatan berjalan dengan baik dan lancar. Evaluasi kegiatan dilihat dari hasil kuisioner yang diisi oleh peserta yang merasa sangat berkesan dan bermanfaat dalam menjelaskan materi bioteknologi. Harapan kedepan dari peserta adanya pelatihan yang lebih advance dalam bidang bioteknologi seperti kultur jaringan, teknologi DNA rekombinan, stem cell, kloning gen dan kultur mikroba di Laboratorium.

Appropriate Concentration of Curcumin as a Growth Factor in Neural Stem Cells Novianti, Titta; Kurniawan, Muhamad Panji Januarsyah; Nainggolan, Ita M
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20862

The growth of Neural Stem Cell (NSC) in adult organisms is limited. Therefore, growth factors are needed to stimulate NSC cell proliferation and differentiation. Herbal Curcumin may be a growth factor. We promoted the growth of Cryopreserved Rat Cortical NSC cells with Curcumin (0.1 µM; 0.5 µM; 1 µM; 2 µM), DMSO, and synthetic growth factors (bFGF, TGF, and heparin). We analyzed the proliferation ability of NSCs by WST-1 assay, cell morphology, and expression of NCS cell marker genes (Nestin, MAP, and Sox2). Morphological analysis showed that cells reproduced big77 optimally at 0.5 µM. The one-way ANOVA and Tukey's posthoc test on the WST-1 test showed significant differences between 0.5 µMCurcumin and other treatment groups. Sox2, MAP-2, and Nestin gene expression peaked at 0.5 µM. The appropriate concentration of Curcumin to stimulate NSC proliferation is 0.5 µM. Herbal extract curcumin has the same effect as commercial growth factors and can substitute synthetic growth factors. Curcumin acts as a growth factor that stimulates the proliferation of mouse NSCs.

PEMANFAATAN PRODUK EKOENZIM SEBAGAI CAIRAN SERBAGUNA RAMAH LINGKUNGAN DALAM AKTIVITAS RUMAH TANGGA Seprianto, Seprianto; Saraswati, Henny; Novianti, Titta; Putri Handayani
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 4 No. 3: Agustus 2024
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v4i3.8223

Keberadaan sampah plastik dari aktivitas rumah tangga menjadi suatu masalah penting karena dapat merusak keseimbangan ekosistem lingkungan dan kesehatan. Ajakan dalam penggunakan produk ecogreen yang ramah lingkungan perlu dianjurkan agar terciptanya lingkungan bersih dan sehat. Masyarakat RW 11 Kelurahan Pamulang Timur, Tangerang Selatan dengan jumlah penduduk yang sangat padat tentu akan menyumbang sampah plastik dari aktivitas rumah tangga. Kurangnya kesadaran masyarakat akan membuang sampah pada tempatnya serta penggunakan produk yang lebih ramah lingkungan tentu akan menimbulkan pemasalahan di lingkungan RW 11 Kelurahan Pamulang Timur. Cara efektif yang dapat direalisasikan pemanfaatan ekoenzim dari fermentasi limbah sayuran dan buah - buahan menjadi produk pembersih alami ramah lingkungan. Penggunakan produk pembersih seperti desinfektan, handwash, karbol, obat antiseptik dari bahan kimia buatan akan meninggalkan residu kimia yang tidak baik untuk kesehatan dan lingkungan. Kegiatan Pengabdian Masyarakat dilaksanakan dengan 2 metode yaitu presentasi dan praktek. Praktek pembuatan spray wajah, shampoo dan sabun cuci tangan menjadi focus utama dalam kegiatan abdimas ini. Peserta sangat antusias mengikuti kegiatan dengan aktif bertanya dan terlibat dalam pembuatan produk ekoenzim. Penggunakan ekoenzim sebagai pengganti cairan pembersih kimia diharapkan dapat memberikan solusi dalam penggunakan produk pembersih yang ecogreen dan ramah lingkungan seperti karbol pembersih alami, serum wajah, sabun cuci piring, pemurni udara, sanitizer alami, pupuk cair, pembersih luka, sehingga dapat mengurangi pemakaian produk berbahan kimia sintetis yang kurang ramah lingkungan sehingga dapat meminilisir produksi sampah plastik.

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