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Detection of the CTX-M Gene Associated with Extended-Spectrum β-Lactamase (ESBL) in Broiler Chickens in Surabaya Traditional Markets Putri, Mariana Febrilianti Resilinda; Khairullah, Aswin Rafif; Effendi, Mustofa Helmi; Wibisono, Freshinta Jellia; Hasib, Abdullah; Moses, Ikechukwu Benjamin; Fauziah, Ima; Kusala, Muhammad Khaliim Jati; Raissa, Ricadonna; Yanestria, Sheila Marty
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.320-334

Abstract

A common indicator used to examine the frequency and distribution of antibiotic resistance against other enteric bacteria in humans and animals is the commensal enteric bacterium, Escherichia coli. The transmission of plasmids harboring ESBL enzymes, primarily generated by E. coli, is the cause of this resistance. The purpose of this study was to identify the CTX-M gene in ESBL-producing E. coli from broiler chicken cloacal swabs in traditional Surabaya markets. The samples used were 96 cloacal swabs from broiler chickens in the traditional markets of Dukuh Kupang, Keputran, Pacar Keling, and Pucang. The antibiotic disks used in this study belonged to five different antibiotic classes; they are aztreonam (monobactam), chloramphenicol (phenicol), kanamycin (aminoglycoside), ciprofloxacin (fluoroquinolone), and tetracycline (tetracycline). Presumptive ESBL strains were then molecularly screened for the presence of CTX-M gene. Results revealed that out of the 96 chicken cloacal swab samples collected, 58 (60.42%) were positive for E. coli based on morphological culture, Gram staining, and biochemical tests. Additionally, 15 out of the 58 E. coli isolates recovered from broiler chicken cloacal swabs were multidrug-resistant (MDR) while 7 of E. coli isolates harbored CTX-M gene. Conclusively, this study has shown that broiler chickens sold in traditional Surabaya markets harbor MDR E. coli which possess CTX-M gene. Conditions in traditional markets with low levels of cleanliness and chickens placed close together can spread resistance genes with serious public health consequences. Therefore, it is imperative to observe good hygienic practices in Surabaya traditional markets in order to curtail the spread of MDR bacterial pathogens in the food chain.
Efek Sari Tomat Ceri (Solanum lycopersicum var.cerasiforme) dalam Pengencer Air Kelapa (Cocos nucifera L.) pada Semenbabi Landrace Foeh, Nancy D. F. K.; Nitbani, Heny; Putri, Mariana Febrilianti Resilinda; Nae, Veronika Serapian Pangu
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.26046

Abstract

The use of fresh semen in artificial insemination quickly leads to a decline in quality if an appropriate extender is not added. A suitable extender functions to protect spermatozoa during processing and storage, thereby prolonging their viability. Additionally, incorporating fruit juices rich in antioxidants can help neutralize free radicals and minimize cellular damage. Semen wascollected froma 2–4-year-old male pig using a dummy. High-quality semen was then divided into two control groups (K0 and K1) and five treatment groups consisting of varying volumes of cherry tomato juice: 20µL, 40µL, 60µL, 80µL, and100µL, all mixed coconut water extender and stored at preservation temperature. Sperm motility was evaluated every two hours for a total of 16 hours. The findings indicated that the coconut water extender combined with cherry tomato juice antioxidants significantly influenced (p<0.05) the motility percentageof Landrace boar spermatozoa. The addition of 60µL cherry tomato juice (Solanum lycopersicum var. cerasiforme) produced the best results in maintaining sperm quality, with a motility value of 67.00±0.79% and a viability duration of up to 16 hours.
Studi Kasus: Identifiksi Patogen Penyebab Penyakit pada Burung Merpati (Columba livia domestica) di Pasar Hewan Kota Surabaya Putri, Mariana Febrilianti Resilinda; Kendek, Irfan Alias; Mandala, Aletha Yuliana; Malelak, Marlin Cindy Claudia; Yanardhini, Ganis; Moekti, Bima Satria
Jurnal Veteriner Nusantara Vol 8 No 2 (2025): Agustus, 2025
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v8i2.26753

Abstract

Pigeons (Columba livia) are a species that often adapt well to urban environments and play an important role in the ecosystem as vectors for the spread of various parasites and zoonotic pathogens. This study aimed to identify the presence of gastrointestinal parasites, blood protozoa, and ectoparasites in pigeons originating from live animal markets in Surabaya, Indonesia. Samples were taken from six pigeons showing clinical symptoms such as diarrhea, anorexia, and weakness. Microscopic examination of feces revealed the presence of Capillaria sp. and Raillietina sp. worm eggs, as well as Eimeria sp. and Haemoproteus columbae protozoan parasites found through blood smears. In addition, ectoparasite identification included Columbicola columbae, Menopon sp., and Menacanthus stramineus. These results show that pigeons in live animal markets have the potential to be a source of parasite and zoonotic disease transmission and have a negative impact on poultry and human health. Therefore, it is important to control parasites through improved sanitation, better habitat management, and vector control to reduce the risk of disease transmission.
EDUKASI PENCEGAHAN RABIES BAGI PEGAWAI PEMERINTAH SEBAGAI UPAYA PENGUATAN LITERASI KESEHATAN DI KECAMATAN MOLLO SELATAN, KABUPATEN TIMOR TENGAH SELATAN Simarmata, Yohanes Timbun Raja Mangihut Ronael; Gelolodo, Maria Aega; Wuhan, Yustinus Oswin Primajuni; Kale, Maria Laurenci Fanny Permata; Putri, Mariana Febrilianti Resilinda; Malelak, Marlin Cindy Claudya
Jurnal Media Tropika Vol 5 No 2 (2025): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v5i2.26418

Abstract