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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Indonesian Journal of Chemistry Jurnal Jamu Indonesia Indonesian Journal of Applied Research (IJAR) Indonesian Journal of Jamu
Septaningsih, Dewi Anggraini
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia; Advanced Research Laboratory, Institute Of Research And Community Services, IPB University, Jl. Palem Kampus
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Engineering Environmental Science Health Professions Medicine & Pharmacology Other

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LC-HRMS-Based Metabolomics for Profiling the Metabolites in Different Plant Parts of Centella asiatica Rafi, Mohamad; Madya, Muhammad Miftahul; Karomah, Alfi Hudatul; Septaningsih, Dewi Anggraini; Ridwan, Taopik; Rohaeti, Eti; Aisyah, Siti; Idroes, Rinaldi
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1106-1115

Abstract

Centella asiatica, or pegagan in Indonesia, is a perennial plant used in Indonesian traditional medicine (jamu) and functional food with many biological activities. Those biological activities come from the bioactive metabolites present in C. asiatica. Differences in metabolite pathways in each part of the plant affect the accumulation of metabolites contained, thus impacting its biological activity. Therefore, this study aims to identify and evaluate differences in the distribution of metabolites in each part of C. asiatica, namely leaves, stems, stolons, and roots. Each plant part was extracted using methanol and sonicated for 30 minutes. The metabolites in the samples were separated and detected using UHPLC-Q-Orbitrap HRMS. Differences in the distribution of metabolites in each part of the plant were evaluated using chemometrics analysis. UHPLC-Q-Orbitrap HRMS analysis could positively identify 37 metabolites, most of which belong to the phenylpropanoid, triterpenoid, triterpenoid saponin, and flavonoid groups. Principal component analysis was able to clearly distinguish each part of the plant using the peak intensity of the overall chromatogram and the peak area of the identified metabolites. The different biosynthetic pathways of metabolites in plants could cause a difference in the distribution of metabolites in each plant.
LC-HRMS-Based Metabolomics Approach Reveals Antioxidant Compounds from Centella asiatica Leaves Extracts Silvia, Riva; Wahyuni, Wulan Tri; Rohaeti, Eti; Aisyah, Siti; Septaningsih, Dewi Anggraini; Karomah, Alfi Hudatul; Rafi, Mohamad
Indonesian Journal of Chemistry Vol 24, No 6 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90782

Abstract

Centella asiatica is a medicinal plant widely used as a traditional medicine due to several biological activities, such as antioxidants in Indonesia. This study aims to identify the active antioxidant compounds of C. asiatica leaves extract using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)-based metabolomics approach. Extracts were prepared using different concentrations of ethanol p.a., i.e. 70% ethanol, 50% ethanol, 30% ethanol, and water. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl method. The results showed the highest antioxidant activity was C. asiatica extracted by 70% ethanol with IC50 of 72.48 ± 2.42 µg/mL. The positive control was ascorbic acid, having an IC50 value of 3.38 ± 0.04 µg/mL. Ascorbic acid and 70% ethanol extract have strong antioxidant activity. Metabolite profiling using LC-MS/MS could identify 35 metabolites consisting of flavonoids, fatty acids, phenolics, terpenes, and several other groups of compounds. Orthogonal partial least squares-discriminant analysis classified the metabolites into active (ethanol 70% and ethanol 50%) and inactive (ethanol p.a., ethanol 30%, and water) antioxidants. Five metabolites have potential as antioxidants, namely 4,5-dicaffeoylquinic acid (11), kaempferol (13), and three unknown compounds.
Total Flavonoids, Antioxidant Capacity, and Metabolite Profiling of Red Ginger (Zingiber officinale var. rubrum) Jahira 1 Variety Purwanto, Ukhradiya Magharaniq Safira; Athaya, Ardelia; Septaningsih, Dewi Anggraini; Safithri, Mega
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.512

Abstract

Red ginger (Zingiber officinale var. rubrum) is traditionally recognized for its medicinal properties, largely due to its antioxidant potential attributed to secondary metabolites such as flavonoids. This study aimed to evaluate the total flavonoid content and antioxidant capacity of ethanol and water extracts of red ginger from Sukabumi, specifically the Jahira 1 variety, as well as identify bioactive compounds. Extraction was carried out using the maceration method. The total flavonoid content was determined using the aluminium chloride colorimetric method, while the antioxidant capacity was assessed via the ferric reducing antioxidant power (FRAP) assay. Results showed that the ethanol extract had higher total flavonoid content and antioxidant capacity, with values of 5.99 mg quercetin equivalent (QE)/g and 473.93 µM Trolox equivalent (TE)/g, respectively. In contrast, the water extract exhibited 3.73 mg QE/g and 206.75 µM TE/g. Further LC-MS/MS analysis of the ethanol extract identified 26 compounds, including phenolics and terpenoids, which contribute to the antioxidant capacity of red ginger from Sukabumi.
Analisis Sidik Jari Kromatografi Lapis Tipis Rimpang Temu Mangga (Curcuma mangga) Makmum Syafi’i; Eti Rohaeti; Wulan Tri Wahyuni; Mohamad Rafi; Dewi Anggraini Septaningsih
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i3.68

Abstract

Profil sidik jari kromatografi lapis tipis (KLT) dapat dimanfaatkan untuk kendali mutu bahan baku maupun produk tumbuhan obat. Penelitian ini bertujuan mengembangkan metode analisis sidik jari rimpang temu mangga (Curcuma mangga) untuk kendali mutunya. Rimpang kering temu mangga diekstraksi menggunakan metanol dan dianalisis sidik jari KLT. Kloroform:etil asetat (8.5:1.5) merupakan fase gerak optimum yang memisahkan 11 pita dari rimpang temu mangga dengan keterpisahan yang baik dan memiliki pita khas biru tua (Rf 0.36). Demetoksikurkumin terdeteksi pada rimpang temu mangga dengan warna jingga kecoklatan pada UV 366 nm (Rf 0.48) setelah diderivatisasi dengan pereaksi asam sulfat 10%. Pengujian spesifitas terhadap rimpang lain dalam satu genus, rimpang temu mangga menunjukan pola yang berbeda. Validasi metode analisis sidik jari KLT memenuhi kriteria keberterimaan, sehingga metode ini dapat digunakan untuk kendali mutu rimpang temu mangga.
Co-Authors Abdul Rohman Abdul Rohman Alfi Hudatul Karomah Alfi Hudatul Karomah Amalia Yunita Athaya, Ardelia Auliatifani, Reza Auliya Ilmiawati Cecep Abdurohman Putra Dien Atin Boritnaban Eti Rohaeti Farit Mochamad Afendi Fifi Gus Dwiyanti Irma Herawati Suparto ISKANDAR ZULKARNAEN SIREGAR Karomah, Alfi Hudatul Khaydanur Khaydanur Khaydanur, Khaydanur Laela Wulansari Latifah Kosim Darusman M. Rafi Madya, Muhammad Miftahul Makmum Syafi’i Maria Bintang Mega Safithri Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Muhamad Insanu Muhammad Majiidu Nanik Siti Aminah Nengsih, Nunuk Kurniati Nunuk Kurniati Nengsih Purnomo, Tsania Firqin Purwanto, Ukhradiya Magharaniq Safira Reza Auliatifani Rinaldi Idroes Rudi Heryanto Sefni Reza Yolanda Silvia, Riva Siti Aisyah SUMINAR SETIATI ACHMADI Taopik Ridwan Tsania Firqin Purnomo Utami Dyah Syafitri Wina Yulianti Wisnu Ananta Kusuma Wulan Tri Wahyuni Wulansari, Laela