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Aplikasi Pemberian Dosis Pupuk Provasoli’s Enriched Seawater (PES) Yang Berbeda Pada Produksi Bibit Gracillaria verrucosa Melalui Kultur Jaringan Dengan Metode Propagasi Vegetatif Rasnijal, Muhammad; Alauddin, Muhammad Hery Riyadi; Budiyati; Anton; Muhammad Syahrir; Yunarty; Saridu, Siti Aisyah; Wahid, Eriyanti; Regan, Yip; Hardianto, Toto; Supryady; Ihwan; Ernawati; Anwar; Mulato, Alwi; Sucipto; Syarief, Muhammad Nurman; Suleman, Yakub; Andini, Salsa; Suleman, Gabriella Augustine; Mulyono, Mugi; Anggoro, Agung Doni; Sektiana, Sinar Pagi; Achmad Suhermanto
JARI : Jurnal Akuakultur Rawa Indonesia Vol. 13 No. 2 (2025): JARI: JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v13i2.71

Abstract

Cultivation of the seaweed Gracilaria verrucosa is necessary as a preventive measure against overexploitation. One applicable method is tissue culture, which requires special attention to nutrient needs to support accelerated growth. These nutrients can be provided through fertilization. One commonly used chemical fertilizer in seaweed tissue culture is Provasoli’s Enriched Seawater (PES). This study aims to determine the optimal PES fertilizer dosage for the production of G. verrucosa seedlings through tissue culture using a vegetative propagation method. This study used a completely randomized design with analysis of variance (ANOVA) consisting of four treatments of different PES fertilizer doses with three replicates for each treatment. The results showed that fertilizer dose variation did not significantly affect the absolute growth of explant weight, but tended to increase the number of growth points. Growth points began to increase in the second week for all treatments, and by the fourth week, the number of growth points at a 1.5% dosage showed a significant difference compared to other doses. The application of PES fertilizer in G. verrucosa seedling production through tissue culture with vegetative propagation indicates that different fertilizer doses do not affect explant growth but significantly influence the increase in growth points by the fourth week
GROWTH OF Thalassiosira sp AND Chaetoceros sp. IN SCALE OF LABORATORY, INTERMEDIATE AND MASSAL Kurniaji, Ardana; Wahid, Eriyanti; Regan, Yip; Rasnijal, Muhammad; Normayanti, Novi
Aurelia Journal Vol 7, No 2 (2025): October
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/aj.v7i2.15093

Abstract

The availability of high-quality live feeds, such as Thalassiosira sp. and Chaetoceros sp., is a critical factor for the success of shrimp aquaculture; however, it is often constrained by fluctuations in growth performance and cell density at different production scales. This study aimed to identify the growth of Thalassiosira sp. and Chaetoceros sp. at laboratory, intermediate, and mass scales. The research was conducted using an experimental method with a Completely Randomized Design (CRD) with two treatments and three replications. The treatments were different microalgae species cultured at different production scales and compared. Cell density data and growth phases were analyzed statistically using SPSS. The results showed that Chaetoceros sp. had higher growth compared to Thalassiosira sp. The cell density range of Chaetoceros sp. was 1.1×10⁷ - 5.0×10⁷ cells/mL, while Thalassiosira sp. was 1.7×10⁶ - 5.2×10⁶ cells/mL. The growth rate of Chaetoceros sp. also reached the peak of the exponential phase faster at Day of Culture (DOC) 4 compared to Thalassiosira sp., which peaked at DOC 5. Water quality during the study across all production scales showed an optimal range for the growth of both microalgae species.