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All Journal Jurnal Medik Veteriner Jurnal Segara Journal of Aquaculture and Fish Health Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Abdi Insani Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Journal of Fish Health Filogeni: Jurnal Mahasiswa Biologi Jurnal Perikanan Jurnal Pendidikan Kimia, Fisika dan Biologi
Maria Agustina Pardede
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Social Sciences Veterinary

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Chloramphenicol Residues and Bacterial Contamination in Farmed African Catfish (Clarias gariepinus) from Banyuwangi Traditional Markets: A Risk Assessment Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Budi, Darmawan Setia; Pardede, Maria Agustina; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.244-254

Abstract

This study aimed to determine chloramphenicol residues and bacterial contamination (Aerobic Plate Count-APC, Escherichia coli count, Salmonella sp., and Vibrio cholerae) in farmed African catfish (Clarias gariepinus) that are marketed in Banyuwangi, Indonesia. A total of ninety samples of C. gariepinus were collected from several markets in Banyuwangi, namely, Kertosari, Blambangan, and Banyuwangi Kota. Using standard procedures, APC, E. coli count, Salmonella sp., V. cholerae, and chloramphenicol residues were determined. 36.37% of samples from Kertosari markets; 23.33% of samples from Banyuwangi Kota markets; and 16.67% of samples from Blambangan markets contained chloramphenicol residues, but less than 0.3 ppb. Only 40% of samples from Kertosari markets, 36.67% of samples from Banyuwangi Kota markets, and 26.67% of samples from Blambangan markets were contaminated by E. coli, but were less than 3 MPNg-1. The highest APC was from Banyuwangi Kota, followed by Kertosari and Blambangan. No samples were contaminated by Salmonella sp. and V. cholerae from any of the markets, and there was no significant difference between all markets in APC, E. coli count, or chloramphenicol residues. All farmed catfish marketed in Banyuwangi were safe to eat because no samples exceeded the maximum chloramphenicol residue and bacterial contaminant standards. Further studies are needed to detect other antibiotic residues used in aquaculture, including tetracycline, sulphonamide, enrofloxacin, and nitrofuran.
CORAL REEF CULTIVATION AT PT. SRI KANDI AQUARIUM, BANYUWANGI: ANALYSIS OF STAGES AND RESULTS OF TRANSPLANTATION of Acropora formosa, Acropora granulosa, and Euphyllia glabrescens Bunayya, Faiq Hanif; Yuniardhi, Dimas Indra; Satria, Rizal Surya; Rahmatulloh, Alfariz Alif; Pardede, Maria Agustina
Jurnal Perikanan Unram Vol 15 No 2 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i2.1405

Abstract

Indonesia is a region known for its abundant natural resources, such as coral reefs. Coral reef ecosystems in Indonesia have begun to decrease, it was recorded that 35.15% of coral reefs in Indonesia entered into poor status or declared damaged. This is due to several factors such as damage caused by the illegal trade of coral reefs carried out by unscrupulous entrepreneurs because of the illegal trade of coral reefs, knowledge and skills are needed regarding transplantation methods in the cultivation of A. formosa, A. granulosa and E. glabrescens to find out the stages and results of transplantation methods in coral reef cultivation. This study was conducted at PT Sri Kandi Aquarium, Banyuwangi Regency, East Java on June 24, 2024 until August 24, 2024. The primary data collection methods used include observation and active participation, while for secondary data using literature studies. The transplantation process in coral reef cultivation consists of several activities, namely the preparation of transplantation tools and materials, making transplantation media, cutting parent fragments, transplantation, maintenance and harvesting. The results of transplantation are then classified based on sizes S, M, L and XL. Thus the transplantation method can be carried out in coral reef cultivation which consists of several stages including preparation of transplantation tools and materials, making transplantation media, cutting coral fragments, attaching the results of cutting fragments to the substrate, sowing corals on the transplantation table under the sea and periodic monitoring and maintenance of corals. The transplantation results obtained will be categorized based on size, namely S, M, L and XL.
Pathology of Silver Rasbora (Rasbora argyrotaenia) after Experi-mentally Infection with Edwardsiella tarda : Edwardsiellosis in Silver Rasbora Nindi, Inda Arsyi; Febrina, Melinda; Rahmawati, Anggi Rusita; Ulkhaq, Mohammad Faizal; Tjahjaningsih, Wahju; Kusdarwati, Rahayu; Pardede, Maria Agustina; Kumalasari, Ditta Putri; Loh, Jiun-Yan
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.70433

Abstract

The high demand for silver rasbora (Rasbora argyrotaenia) from the wild had led to declining resources, making fish cultivation necessary. One of the challenges in fish farming is diseases caused by bacteria, such as Edwardsiella tarda, which can lead to Edwardsiellosis and result in mass mortality among fish. This study aimed to analyze the pathology of silver rasbora after experimental infection with E. tarda. Four hundred silver rasbora (5.4±7 cm and 0.47±2.63 g) were reared in twenty aquariums. The treatments consisted of four groups with five replicates, including immersion in E. tarda 1011 CFU/ml (P1), 1012 CFU/ml (P2), 1013 CFU/ml (P3), and a negative control (without E. tarda) (P0) for a 14-days rearing period. Parameters observed included survival rate, stress responses (plasma cortisol, blood glucose, and ventilation rate); hematology profile (total erythrocytes, total leukocytes, differential leukocytes, and hemoglobin levels), histopathological alterations in organs, survival rate, and clinical symptoms. The results showed that immersion in E. tarda did not affect fish mortality. However, other parameters, including hematological profile, stress responses, and histopathological alterations, showed increased values and several pathological changes, yet remained within normal limits.
Pemeriksaan EHP dan AHPND pada Litopenaeus vannamei dengan Metode PCR di BKHIT Jawa Timur Maria Agustina Pardede; Silvani Tri Julianti; Putri Puspita Sari; Hesti Listiyani; Dewi Kartika; Siti Mufidah; Muhammad Adrian Fauzi; Mohamad Fathoni
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 2 No. 3 (2026): Mei : Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v1i4.489

Abstract

White shrimp (Litopenaeus vannamei) is an introduced species cultivated in Indonesia and plays an important role in increasing national shrimp production and exports. However, infectious diseases such as Enterocytozoon hepatopenaei (EHP) and Acute Hepatopancreatic Necrosis Disease (AHPND) pose a serious threat to the shrimp farming industry. These two diseases cause a decrease in shrimp growth and production performance. The Polymerase Chain Reaction (PCR) diagnostic technique is an effective method for detecting EHP and AHPND infections, because the results are fast and accurate. This research was carried out at the Juanda Fish Quarantine Laboratory, Animal, Fish and Plant Quarantine Center (BKHIT) East Java from July 1 to August 29 2024. This research aims to find out examination techniques and examination results for Enterocytozoon hepatopenaei (EHP) and Acute Hepatopancreatic Necrosis Disease (AHPND) during the examination process at BKHIT East Java. The test method used is a descriptive method by collecting primary and secondary data. The EHP examination at BKHIT East Java uses the single step PCR method, while the AHPND examination uses the Nested PCR method which consists of extraction, amplification, electrophoresis and visualization of the results. Based on the results of examinations during the activity, 4 samples were declared positive for EHP and no samples were declared positive for AHPND out of 51 samples of white shrimp (Litopenaeus vannamei).
Peningkatan asupan protein hewani santri melalui edukasi gizi dan budidaya ikan di pondok pesantren Fasya, Arif Habib; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono; Pardede, Maria Agustina; Kumalaningrum, Dwi Retna; Prayogo; Mauludia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy; Ambarwati, Dewi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 6 No. 3 (2025)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v6i3.23872

Abstract

Pengabdian kepada masyratakat ini dilaksanakan di Pondok Pesantren Al Anwari, Banyuwangi sebagai upaya untuk memenuhi kebutuhan protein hewani bagi para santri melalui budidaya ikan lele. Tujuan dari kegiatan ini adalah untuk memenuhi asupan protein hewani pada pondok pesantren serta  meningkatkan pengetahuan dan keterampilan santri dalam aspek teknis budidaya meliputi manajemen pakan hingga pasca panen. Metode kegiatan meliputi lima tahap yaitu penjaringan aspirasi, pelatihan, pendampingan, monitoring dan evaluasi serta dilakukan pengukuran kualitas air serta pretest maupun posttest. Pelaksanaan kegiatan melibatkan 49 santri kelas IX bersama pengelola pondok pesantren. Hasil pengukuran kualitas air menunjukkan nilai berada dalam kisaran normal untuk budidaya ikan lele. Nilai pretest berkisar antara 86,64-94,74 meningkat menjadi 88,60-96,49 pada posttest. Hasil ini menunjukkan bahwa pendekatan berbasis praktik dan pendampingan efektif dalam meningkatkan pengetahuan peserta. Kegiatan ini berpotensi dilakukan di lembaga pendidikan berbasis pesantren lainnya untuk mendukung ketahanan pangan dan meningkatkan ketrampilan santri.
Dietary Honeysuckle (Lonicera japonica) Extract Enhances Resistance to Infectious Myonecrosis Virus in Whiteleg Shrimp (Litopenaeus vannamei) Subaidah, Siti; Sumarwan, Joko; Marzuqi, Muhammad; Tridjoko, Tridjoko; Slamet, Bejo; Budi, Darmawan Setia; Pardede, Maria Agustina; Ulkhaq, Mohammad Faizal; Imlani, Ainulyakin; Loh, Jiun-Yan
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.286-296

Abstract

This study aimed to investigate the immune response of whiteleg shrimp (Litopenaeus vannamei) to prevent infectious myonecrosis virus (IMNV) infection by incorporating Lonicera japonica water extract into artificial feed. A total of 180 whiteleg shrimp were used in four treatment groups (triplicate) receiving different doses of L. japonica extract in their feed, including treatment A (1% w/w), B (2% w/w), C (3% w/w), and D (without supplementation/control). The dietary treatments were applied twice daily for two weeks. On the 15th day, the shrimp were injected intramuscularly at the fifth abdominal segment with IMNV isolates (100 µL/shrimp) and observed for seven days post-infection (dpi). The parameters observed included hemolymph profile, survival rate, gross clinical signs, and water quality. IMNV infection was confirmed through both external clinical signs and PCR tests. The results showed that the addition of L. japonica water extract to the feed improved the survival rate and immune responses of whiteleg shrimp following the IMNV challenge test. In conclusion, dietary supplementation of L. japonica water extract at a concentration of 2% w/w in artificial feed could serve as a preventive agent against IMNV infection in whiteleg shrimp.
IDENTIFICATION, ISOLATION, PURIFICATION, GRAM STAINING, AND BIOCHEMICHAL TESTING PROCESSES OF AQUATIC BACTERIA FROM DIFERENT SOURCES Pardede, Maria Agustina; Fasya, Arif Habib; Kumalaningrum, Dwi Retna; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Ambarwati, Dewi; Maulana, Muhammad Hilmy
Jurnal Segara Vol 19, No 3 (2024): December
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/segara.v19i3.15409

Abstract

Abstract
Economic improvement and fish farming based on Smart Aquaculture Automatized System in Segobang Village, Banyuwangi Arif Habib Fasya; Hapsari Kenconojati; Darmawan Setia Budi; Suciyono Suciyono; Maria Agustina Pardede; Dwi Retna Kumalaningrum; Prayogo Prayogo; Eka Saputra; Aji Akbar Firdaus; Muhammad Hilmy Maulana; Dewi Ambarwati
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 10 No. 1 (2025): February 2025
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v10i1.14811

Abstract

The fisheries and marine sector in East Java has abundant potential, one of which is Banyuwangi district. Banyuwangi Regency has potential in freshwater aquaculture because it has 324 rivers with an area of 735 km2 and swamps and reservoirs of 60 hectares. One of the villages in Banyuwangi Regency is Segobang Village, which has the potential to have abundant water sources and continuous flow. This potential can be utilized by training and assisting fish nurseries in Segobang Village by utilizing continuously flowing water and Smart Aquaculture Automatized System (SAAS) technology by utilizing biofilters and running water and aeration as a source of oxygen.  The stages carried out in this community service are as follows: initial assessment of the location, infrastructure and technology development, periodic training and mentoring, implementation and monitoring of the cultivation process, and continued marketing and sales. After the training, the Segobang Village community showed a significant increase in knowledge. The average pre-test scores ranged from 30 to 51, while the post-test scores increased to 79 to 87. This improvement shows that participants better understand the SAAS and its use in fish farming. Continued training and practice can help strengthen the adoption of this technology in the community.
of, from, in, the IDENTIFIKASI PARASIT IKAN POGOT DARI TANGKAPAN ALAM PERAIRAN SELAT BALI Sya'adah, St. Risma; Pratiwi, Serli Anjelica; Safira, Ayu; Febrianto, Dikki; Bintang, Muhammad Nur; Pardede, Maria Agustina; Ambarwati, Dewi
Filogeni: Jurnal Mahasiswa Biologi Vol 6 No 1 (2026): Januari-April
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/filogeni.v6i1.59610

Abstract

Ikan pogot (Odonus niger dan Melichthys niger) merupakan salah satu jenis ikan karang yang ditemukan di perairan Selat Bali, Indonesia. Ikan ini memiliki potensi untuk dibudidayakan, namun rentan terhadap infeksi parasit yang dapat memengaruhi kualitasnya. Penelitian ini bertujuan untuk mengidentifikasi parasit yang menginfeksi ikan pogot sehingga dapat memberikan informasi yang bermanfaat bagi pembudidaya dalam pencegahan dan penanganan. Sebanyak 48 ekor ikan pogot ditangkap dari perairan Selat Bali dan diperiksa secara makroskopis serta mikroskopis menggunakan teknik scraping dan biopsi. Hasil penelitian menunjukkan adanya tiga jenis parasit, yaitu Chilodonella sp., Diplectanum sp., dan Neoechinorhynchus sp., dengan Diplectanum sp. sebagai jenis yang paling dominan dan terdeteksi pada insang ikan. Gejala klinis yang muncul pada ikan terinfeksi meliputi kesulitan bernapas serta perubahan warna insang menjadi pucat. Analisis prevalensi dan intensitas menunjukkan bahwa Diplectanum sp. pada O. niger memiliki prevalensi 30% (umum) dengan intensitas 0,14 ind/ekor (sangat rendah), sedangkan pada M. niger prevalensinya 20% (sering) dengan intensitas 0,2 ind/ekor (sangat rendah). Neoechinorhynchus sp. hanya ditemukan pada M. niger dengan prevalensi 12% (sering) dan intensitas 0,33 ind/ekor (sangat rendah). Sementara itu, O. niger juga terinfeksi dengan prevalensi 17% (sering) dan intensitas 0,25 ind/ekor (sangat rendah). Temuan tersebut mengindikasikan bahwa infeksi parasit pada ikan pogot di Selat Bali tergolong rendah, namun tetap perlu diwaspadai karena berpotensi memengaruhi kesehatan ikan dan keberhasilan budidaya.
EDUKASI TENTANG WABAH DAN PENCEGAHAN PENYAKIT LELE KUNING DI KELOMPOK PEMBUDIDAYA IKAN KARYA MINA LELE KECAMATAN MUNCAR, KABUPATEN BANYUWANGI, JAWA TIMUR Maria Agustina Pardede; Prayogo Prayogo; Dwi Retna Kumalaningrum; Mohhamad Faizal Ulkhaq; Dewi Ambarwati; Mohammad Hilmy Maulana; Shierly Zaissiliya Clarissa; Yunita Ika Putri Puspitasari; Farhan Gymnastiar Baihaqi; Nirmala Sagita; Octaviano Ramadhan
Jurnal Abdi Insani Vol 13 No 6 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i6.4004

Abstract

Catfish (Clarias sp.) is a major freshwater aquaculture commodity with high production value in Indonesia. Intensification using Recirculating Aquaculture Systems (RAS) has increased disease risks, including jaundice, which is associated with high mortality rates. Jaundice in fish is generally linked to impaired liver function and disruption of bilirubin metabolism, leading to abnormal pigment accumulation and yellow discoloration. Limited farmer knowledge and improper management practices further exacerbate disease outbreaks and production losses. This activity aimed to improve farmers’ understanding of the causes, early detection, and prevention of jaundice disease to enhance fish health and aquaculture productivity. The program was conducted through systematic counseling, training on early detection and field diagnosis, and practical implementation of sanitation, water quality management, biosecurity, and fish quarantine. Evaluation was performed using pre-test, post-test, and regular monitoring of culture conditions. The results demonstrated a substantial improvement in farmers’ knowledge and skills in recognizing clinical symptoms and conducting early disease detection. The application of sanitation and biosecurity measures improved water quality and reduced environmental risk factors. Most ponds showed a decrease in disease outbreaks and mortality rates. Fish survival rates increased compared to pre-intervention conditions, indicating the effectiveness of the implemented strategies. Additionally, farmers became more capable of independently applying fish health management practices, including feed management, water quality control, and preventive measures against disease transmission. This program effectively enhanced farmers’ capacity in preventing jaundice, contributing to improved fish health and aquaculture productivity.
Co-Authors Alin Asyabil Ambarwati, Dewi Ayu Safira Ayu Safira, Ayu Bejo Slamet Bintang, Muhammad Nur Bunayya, Faiq Hanif DARMAWAN SETIA BUDI Dewi Ambarwati Dewi Ambarwati Dewi Kartika Dwi Retna Kumalaningrum Dwi Retna Kumalaningrum Eka Saputra Farhan Gymnastiar Baihaqi Fasya, Arif Habib Febrianto, Dikki Febrina, Melinda Fika Zahroh Firdaus, Aji Akbar Hesti Listiyani Imlani, Ainulyakin Joko Sumarwan, Joko Kenconojati, Hapsari Kumalasari, Ditta Putri Loh, Jiun-Yan Maulana, Muhammad Hilmy Mauludia, Farida Mohamad Fathoni Mohammad Faizal Ulkhaq Mohammad Hilmy Maulana Mohhamad Faizal Ulkhaq Muhammad Adrian Fauzi Muhammad Marzuqi Nindi, Inda Arsyi Nirmala Sagita Noerita Widya Melati Octaviano Ramadhan Pratiwi, Serli Anjelica Prayogo Prayogo Prayogo Prayogo Prayogo Putri Puspita sari Rahmatulloh, Alfariz Alif Rahmawati, Anggi Rusita Rama Nia Julia Putri Rentienna Rachmadhany Satria, Rizal Surya Shierly Zaissiliya Clarissa Shierly Zaissiliya Clarissa Lifani Silvani Tri Julianti Siti Mufidah Siti Subaidah Suciyono Suciyono, Suciyono Sya'adah, St. Risma Tjahjaningsih, Wahju Tridjoko Tridjoko Yuniardhi, Dimas Indra Yunita Ika Putri Puspitasari Yunita Ika Putri Puspitasari