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All Journal The Indonesian Biomedical Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Kesehatan Tambusai Jurnal Widya Medika
Irene Lingkan Parengkuan
Faculty Of Medicine, Widya Mandala Catholic University, Surabaya
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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Correlation Between IFNγ+ and CTLA-4+ Tumor Infiltrating Lymphocytes In Luminal And Non-Luminal Breast Carcinoma Irene Lingkan Parengkuan; Sjahjenny Mustokoweni; Nila Kurniasari
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 3, No 2 (2019)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1539.508 KB) | DOI: 10.30651/jqm.v3i2.2778

Abstract

ABSTRACT The role of tumor infiltrating lymphocytes (TIL) in breast carcinoma depends on the molecular subtype, especially the expression of the estrogen receptor. A greater mutation load in the non-luminal subtype leads to continuous activation of the immune system resulting in exhausted T lymphocytes. Observational research with a cross-sectional approach was conducted on 40 formalin fixed paraffin-embedded tissue from breast carcinoma at the Anatomical Pathology Laboratory of Dr. Soetomo General Hospital Surabaya during January 2017 -  December 2018. Samples were divided into two groups based on their status of ER expression. The parameter was a positive percentage of TIL immunoreactivity against IFNγ and CTLA-4 antibodies. Percentage of IFNγ+ TIL is higher in the luminal subtype (p =0.001), whereas the percentage of CTLA-4+ TIL is higher in the non-luminal (p =0.001). These expressions were significantly correlated with the molecular subtype of breast carcinoma (p=0.001). A significant correlation between IFNγ+ and CTLA-4+ TIL were found (rs=-0.350, p=0.027). Exhausted T lymphocytes express some inhibitor molecules such as CTLA-4. CTLA-4 (Cytotoxic T-Cell Lymphocyte Associated Protein-4) suppresses immune system function including the activity of IFN-γ as an important molecule in anti-tumor immunity and forms an immunosuppressive and pro-tumor microenvironment. Different level of expressions of IFNγ+ (p=0.001) and CTLA-4 + (p=0.001) TIL were proven to be related to the molecular subtype of breast carcinoma (rs=-0.683, p=0.001; rs=0,501, p=0.001, respectively). The negative correlation between IFNγ+ and CTLA-4+ TIL shows the role of CTLA-4 as an inhibitory molecule to the immune system (rs=-0.350, p=0.027).Keywords : tumour infiltrating lymphocytes, IFNγ, CTLA-4, breast carcinoma,  luminal molecular subtypeCorrespondence to: lingkan.parengkuan@gmail.com ABSTRAK Peran tumour infiltrating lymphocytes (TIL) pada karsinoma payudara berhubungan erat dengan subtipe molekuler, terutama ada atau tidaknya ekspresi reseptor estrogen. Beban mutasi yang besar pada subtipe non-luminal menyebabkan pengaktifan sistem imun terjadi terus menerus dengan hasil akhir terbentuknya subset limfosit T yang kelelahan. Penelitian observasional analitik dengan pendekatan potong lintang ini menggunakan sampel 40 blok parafin dari penderita karsinoma payudara di Laboratorium Patologi Anatomi RSUD Dr. Soetomo Surabaya periode 1 Januari 2017 – 31 Desember 2018 yang dibagi menjadi 2 kelompok berdasarkan ada atau tidaknya ekspresi reseptor estrogen. Parameter penilaian adalah jumlah persentase TIL area tumor invasif yang terpulas positif dengan antibodi IFNγ dan CTLA-4. Ekspresi IFNγ+ TIL didapatkan lebih tinggi pada subtipe luminal (p=0,001), sedangkan ekspresi CTLA-4+ TIL didapatkan lebih tinggi pada subtipe non-luminal (p=0,001). Analisis statistik menunjukkan adanya korelasi signifikan antara ekspresi IFNγ+ TIL dengan CTLA-4+ TIL (rs=-0,350, p=0,027). Salah satu sifat dari sel limfosit T kelelahan adalah mengekspresikan molekul inhibitor sistem imun antara lain CTLA-4 (Cytotoxic T-Cell Lymphocyte Associated Protein-4). CTLA-4 akan menekan fungsi sistem imun dan berdampak pada penurunan aktivitas IFN-γ yang merupakan salah satu molekul penting dalam imunitas anti-tumor sehingga terbentuklah lingkungan mikro yang imunosupresif dan pro-tumor. Hasil penelitian menunjukkan adanya perbedaan ekspresi IFNγ+ TIL (p=0,001) dan CTLA-4+ TIL (p=0,001) pada kedua kelompok yang secara bermakna berhubungan dengan subtipe molekuler karsinoma payudara (secara berurutan mendapatkan nilai (rs=-0,683, p=0,001 dan rs=0,501, p=0,001). Korelasi negatif antara IFNγ+ TIL dengan CTLA-4+ TIL menunjukkan adanya peran CTLA-4 sebagai molekul inhibisi terhadap sistem imun (rs=-0,350, p=0,027).Kata kunci                  : tumour infiltrating lymphocytes, IFNγ, CTLA-4, karsinoma payudara, subtipe molekuler luminalKorespondensi            :lingkan.parengkuan@gmail.com
Intravascular Lymphoma, Jenis Limfoma Yang Jarang Dijumpai Dengan Prognosis Buruk Willy Sandhika; Aries Sasongko; Irene Lingkan Parengkuan
JURNAL WIDYA MEDIKA Vol. 5 No. 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i2.2205

Abstract

Intravascular lymphoma is a rare type of lymphoma with some fundamental differences compared to other types of non–Hodgkin lymphoma in general. Its clinical manifestations vary greatly and depend on the affected organs. There is no pathognomonic signs of intravascular lymphoma, either in clinical, laboratory or radiological findings. This case is challenging to detect and often misdiagnosed because it does not show symptoms that are commonly found in other types of lymphoma, such as mass or nodules formation. The treatment is relatively similar to other lymphoma in general, but the prognosis is worse because it was mostly diagnosed in the advanced stage. These specific conditions become the main reason to separate this case as a special entity of lymphoma.
EFFECT OF MICROPLASTIC INTAKE ON INTESTINAL AND PANCREATIC CELL DAMAGE Steven Wijono; Irene Lingkan Parengkuan; Shella Morina; Vincentius Diamantino Supit; David Karunia Jaya; Leonardo Suryanto Wicaksono; Michael Christian Iskandar
JURNAL WIDYA MEDIKA Vol. 8 No. 2 (2022): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v8i2.4131

Abstract

Introduction: Microplastics are plastic particles that result from the breakdown of larger plastic particles into smaller pieces and are found in natural environments such as oceans, beaches, and land. Microplastics harm the environment and affect human health. The main entrance of microplastics into the body is the digestive system, through the food and drinks we consume daily. Various investigations have shown that human feces samples contain microplastics that come from ingestion of contaminated food. If it continues, it can damage our body cells. Objective: This research aims to demonstrate that oral administration of microplastics can impair the function of the small intestine, large intestine, and pancreas in rattus of the strain Rattus norvegicus wistar. Method: This study is a quantitative analytic investigation employing an experimental methodology on experimental animals. In this work, the experimental animals were separated into six groups, including the control group and the treatment groups X1, X2, X3, X4, and X5; microscopic observations were conducted 90 days after the microplastics were administered. Results: The comparison of the control group with each exposure group to the small intestine revealed significant results in the Pearson correlation test in groups K with X2, X3, and X4 and the Mann-Whitney difference test in groups K with X2 and X4. Comparing the control group with each exposure group to the large intestine revealed no significant results in the Pearson correlation test and the Mann-Whitney difference test. Conclusion: The correlation test results between the control group and the complete exposure groups revealed significant outcomes in the small intestinal tissue but not in the large intestine and pancreas tissue.
Modified High-Fat High-Sucrose Diet Promotes Obesity and Alters Colonic Cytokines Novita, Bernadette Dian; Wedharga, I Gede Putu Adhi; Tjahjono, Yudy; Wijaya, Hendy; Theodora, Imelda; Ervina, Martha; Wilianto, Yufita Ratnasari; Dewi, Sianty; Parengkuan, Irene Lingkan; Herjunianto, Herjunianto; Ghasani, Sabrina Maria; Limantoro, Michael Christian; Jaya, Ferdinand Wiliam
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3807

Abstract

BACKGROUND: Western dietary patterns are often associated with increasing non-communicable diseases, including inflammatory bowel disease (IBD). In experimental models, a high-fat high-sucrose (HFHS) diet is used to mimic metabolic and inflammatory effects of such diets, however the data regarding colonic inflammation in Indonesia remain limited. Therefore, this study was conducted evaluated the impact of HFHS feeding on colonic interleukin (IL)-6, IL-10 expression, and the IL-6/IL-10 ratio.METHODS: Six weeks old male C57BL/6 mice were randomly assigned to a normal fat diet (NFD) or HFHS diet group and fed ad libitum for 8 weeks. Colonic tissues were collected, and IL-6 and IL-10 expression was analyzed by immunohistochemistry.RESULTS: HFHS-fed mice showed significant increases in body weight (increased by 22.44%, p=0.0047) and caloric intake (increased by 125.17%, p=0.0000), confirming obesity induction. Colitis was also evident, with higher histological colitis scores (p=0.0072). However, colonic IL-6 (increased by 9.12%, p=0.1236), IL-10 (increased by 1.49%, p=0.8013), and the IL-6/IL-10 ratio (increased by 7.38%, p=0.4000) showed no significant differences compared to NFD.CONCLUSION: In C57BL/6 mice, an 8-week modified HFHS diet induced obesity, increased caloric intake, and mucosal injury, but did not significantly alter colonic IL-6, IL-10, or their ratio. This suggests preserved mucosal immune homeostasis consistent with an early compensatory phase rather than overt cytokine-driven inflammation. Longer or more intensive exposure may disrupt this balance, highlighting the need for further studies to define the temporal threshold and clarify immune microbiome interactions in colitis progression.KEYWORDS: high-fat high-sucrose diet, colon inflammation, IL-6, IL-10, obesity mice
PENGARUH PARTIKEL MIKROPLASTIK DALAM DARAH TERHADAP KADAR INDEKS SERUM PENILAIAN FUNGSI GINJAL Simanjuntak, Angeline Rivia; Sincihu, Yudhiakuari; Parengkuan, Irene Lingkan
Jurnal Kesehatan Tambusai Vol. 5 No. 1 (2024): MARET 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i1.23956

Abstract

Baru-baru ini beberapa hasil penelitian menduga mikroplastik sebagai salah satu faktor risiko terjadinya gangguan ginjal. Mikroplastik merupakan fragmen hasil degradasi plastik yang berukuran lebih besar. Di dalam tubuh, mikroplastik akan terdistribusi ke seluruh tubuh sehingga memungkinkannya terakumulasi di suatu organ. Akumulasi mikroplastik dapat memicu terjadinya stres oksidatif, sitotoksitas, dan inflamasi yang berujung pada terganggunya fungsi suatu organ. Serum albumin, serum kreatinin, dan blood urea nitrogen merupakan biomarker yang masih rutin digunakan untuk menilai fungsi ginjal. Tujuan penelitian ini adalah untuk menjelaskan pengaruh partikel mikroplastik dalam darah terhadap kadar serum albumin, serum kreatinin dan blood urea nitrogen pada Rattus norvegicus Strain Wistar. Penelitian ini membagi 30 ekor Rattus norvegicus Strain Wistar ke dalam 1 kelompok kontrol dan 5 kelompok eksperimen. Kelompok eksperimen diberikan paparan mikroplastik peroral dengan dosis berbeda mulai dari 0,0375 mg/hari; 0,075 mg/hari; 0,15 mg/hari; 0,3 mg/hari; dan 0,6 mg/hari selama 90 hari. Hasil uji analisis data secara statistika menunjukkan adanya keterdugaan korelasi atau pengaruh partikel mikroplastik dalam darah terhadap kadar serum albumin (P = 0,009; R = -0,466) dan blood urea nitrogen (P = 0,010; R = 0,460), serta tidak adanya korelasi atau pengaruh partikel mikroplastik dalam darah terhadap kadar serum kreatinin (P = 0,418). Dapat disimpulkan bahwa pemberian paparan mikroplastik peroral selama 90 hari pada Rattus norvegicus Strain Wistar diduga dapat menyebabkan penurunan kadar serum albumin dan peningkatan blood urea nitrogen. Sedangkan kadar serum kreatinin didapatkan hasil yang hampir sama antara kelompok kontrol dan kelompok eksperimen.
Co-Authors Aries Sasongko David Karunia Jaya Ghasani, Sabrina Maria Herjunianto Herjunianto Jaya, Ferdinand Wiliam Leonardo Suryanto Wicaksono Limantoro, Michael Christian Martha Ervina Michael Christian Iskandar Nila Kurniasari Novita, Bernadette Dian Shella Morina Sianty Dewi Simanjuntak, Angeline Rivia Sincihu, Yudhiakuari Sjahjenny Mustokoweni, Sjahjenny Steven, . Theodora, Imelda Tjahjono, Yudy Vincentius Diamantino Supit Wedharga, I Gede Putu Adhi Wijaya, Hendy Wilianto, Yufita Ratnasari Willy Sandhika