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All Journal Journal of Tropical Animal Science and Technology Jurnal Nukleus Peternakan Jurnal Veteriner Nusantara Journal of Innovative and Creativity
Bette, Yustiany Y
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Public Health Social Sciences Veterinary Other

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EVALUASI KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER SPERMAX MODIFIKASI DENGAN WAKTU PRE-FREEZING YANG BERBEDA (Assessment of Landrace Boar Frozen Semen Quality in Modified Spermax Diluent Under Varying Pre-freezing Durations) Kii, Mariana B.I.; Hine, Thomas M; Bette, Yustiany Y; Nalley, Wilmientje M
JURNAL NUKLEUS PETERNAKAN Vol 12 No 1 (2025): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v12i1.22385

Abstract

Research was conducted to investigate the impact of different pre-freezing durations (PF) using a modified spermax (M) extender on the quality of frozen landrace boar semen. The study utilized fresh semen from four healthy Landrace boars aged 2-3 years. Fresh semen meeting SNI standards was diluted in SM extender and held at room temperature (27-28℃) for two hours. Subsequently, the semen was centrifuged at 2000 rpm for 10 minutes, the supernatant was discarded, and the sperm pellet was re-diluted with SM extender at a 1:1 ratio. The semen was then packaged in 0.5 mL straws, placed on a freezing rack, and allowed to equilibrate for two hours at 3-5℃. Following equilibration, the semen was re-evaluated, and the straws were frozen above liquid nitrogen at -110℃ with varying pre-freezing times of 5, 8, 11, and 14 minutes. After the pre-freezing period, the straws were plunged into liquid nitrogen for final freezing and then stored in liquid nitrogen containers at -196℃. The results indicated that the duration of pre-freezing time did not lead to statistically significant differences (P>0,05) in sperm motility, viability, abnormality, and recovery rate (RR). In conclusion, pre-freezing times ranging from 5 to 14 minutes had a similar impact on the quality of frozen landrace boar semen.
Pengaruh penambahan sari daun binahong kedalam pengencer Tris kuning telur terhadap kualitas semen cair Babi persilangan landrace dan duroc Kota, Ino; Riwu, Agustinus Ridlof; Bette, Yustiany Yuliana
Jurnal Veteriner Nusantara Vol 8 No 2 (2025): Agustus, 2025
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v8i2.24883

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan ekstrak daun binahong (SDB) dalam pengencer telur tris yolting (T-kt) terhadap kualitas spermatozoa babi hasil persilangan landrace x duroc. Bahan yang digunakan dalam penelitian ini adalah semen segar dari babi hasil persilangan landrace x duroc umur 1,5 tahun dalam kondisi sehat dan telah dibor untuk koleksi semen. Penelitian ini menerapkan metode eksperimen dengan perencanaan acak lengkap (RAL) yang melibatkan 6 perlakuan dan 5 ulangan, yaitu: P0 = T-KT + SDB, 0%, P1 = T-KT + SDB, 0,5% P2 = T-KT + SDB, 1% P3 = T-KT + SDB, 1,5% P4 = T-KT + SDB, 2%, P5 = T-KT + SDB, 2,5%. Variabel yang diteliti adalah motilitas, viabilitas, abnormalitas, dan viabilitas spermatozoa. Semen yang telah encer disimpan dalam pendingin pada suhu 15-20℃ dan dievaluasi setiap 12 jam. Berdasarkan hasil penelitian, dapat disimpulkan bahwa perlakuan P2 dengan konsentrasi 1% (SDB) menunjukkan motilitas 57,00±2,74%, viabilitas 66,70±3,90%, abnormalitas 5,00±0,61%, dan rentang hidup 56,20±1,54 jam.
Quality Of Frozen Cement From Landrace Boars In Modified Citrate Diluents With Different Glycerol Concentrations Yohanes Paulus Martinus Bay; Thomas Mata Hine; Yustiany Yuliani Bette; W. Marlene Nalley
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4269

Abstract

The purpose of this study was to determine the quality of frozen semen from landrace boars in modified citrate diluents with different glycerol concentrations. The research material used was fresh cement from 2-3 year old landrace boar studs from 4 boars. This study used an experimental study with a completely randomized design method consisting of 4 treatments and 4 replications. The treatments in question are T1: S-KT + G 3%, T2: S-KT + G 5%, T3: S-KT + G 7% and T4: S-KT + G 9%. The diluted cement was stored at room temperature (27-28℃) for two hours (holding time), then centrifuged at 3000 RPM for 15 minutes. After centrifugation, the supernatant was discarded and the centrifuged pellet was re-diluted with basic diluent. The diluted semen was packed in 0.5 Ml straws, then packed, arranged in a freezing rack, and then equilibrated at 3-5℃ for 2 hours. Then the straws were frozen above the surface of liquid N2 with a distance of 10 cm at a temperature of ±-110℃ for 10 minutes. The results of this study indicate that the addition of different glycerols did not have a significant effect (P>0.05) on the motility, viability, abnormality and recovery rate of spermatozoa. It was concluded that the addition of different glycerols had the same impact but were not suitable for IB.
Pengaruh Penambahan Sari Daun Pepaya Dalam Pengencer Sitrat Kuning Telur Terhadap Kualitas Spermatozoa Babi Persilangan Landrace Dan Duroc Apolonius Grad Suardi; Kirenius Uly; Aloysius Marawali; Yustiany Yuliana Bette
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4124

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh penambahan sari daun papaya (SDP) ke dalam pengencer Tris-kuning Telur (T-KT) terhadap kualitas semen babi persilangan landrace dan duroc. Materi yang digunakan adalah semen segar dari babi jantan persilangan landrace dan duroc berumur 1,5 tahun dengan kondisi sehat dan terlatih untuk penampungan semen. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap yang terdiri atas enam perlakuan dan lima ulangan, yaitu: T-KT (P0), SDP, (P1) T-KT+0,5% SDP, (P2) T-KT+1% SDP, (P3), T-KT+1,5% SDP (P4), T-KT+2% SDP, dan (P5) TKT+2,5% SDP. Semua perlakuan disimpan dalam coolbox pada suhu 15–20ºC. Variabel yang dilihat adalah motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa. Evaluasi dilakukan setiap 12 jam. Hasil penelitian menunjukkan bahwa perlakuan P5 (penambahan SDP 2,5%) pada penyimpanan selama 36 jam memberikan hasil terbaik (P<0,05) dibandingkan dengan perlakuan lainnya, dengan nilai motilitas 44,00±2,23% viabilitas 51,90±2,63%, abnormalitas 5,50±0,70%, dan daya tahan hidup selama 45,60 jam. Disimpulkan bahwa penambahan 2,5% SDP dalam pengencer Tris-kuning telur menghasilkan kualitas semen cair babi persilangan yang lebih baik dibandingkan perlakuan lainnya.
Correlation Between Chest Girth, Body Length, and Body Height on Wither Weight of Rote Sheep Ewes Setyani, Ni Made Paramita; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Riwu, Agustinus Ridlof; Uly, Kirenius; Kune, Petrus; Bette, Yustiany Yuliana; Lawa, Alvrado Bire; Mulik, Simon Edison; Banamtuan, Adyanto Nessy; Riwu, Roni Markus Jami; Zulfa, Yanna Nailla; Listyadi, Rut
Journal of Tropical Animal Science and Technology Vol. 8 No. 1 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i1.10617

Abstract

This study aimed to analyze the relationship between linear body measurements including chest girth (CG), body length (BL), and wither height (WH) and body weight (BW) in Rote sheep ewes across different age groups to determine the most reliable predictor of body weight. The research employed a quantitative method through direct field measurements and weighing. Data collected including vital statistics (CG, BL, WH) and BW from Rote sheep ewes categorized into three age groups (I0, I2, and I4). The data were analyzed statistically using correlation coefficient (r), the coefficient of determination (R²), and bias analysis to evaluate the accuracy of weight estimation. The results revealed that chest girth exhibited the highest correlation with body weight, particularly in the I0 group (r = 0.93; R² = 86.05%), indicating a strong positive relationship and high predictive accuracy. Body length showed a moderate relationship (r = 0.87; R² = 76.11%) in the same group, while wither height demonstrated a relatively weaker correlation (r = 0.73; R² = 53.94%). Across all age groups, the predictive strength of body measurements decreased with increasing age, suggesting that skeletal growth contributes less to body weight variation in mature sheep. In conclusion, chest girth was identified as the most accurate and reliable linear measurement for predicting body weight in Rote sheep ewes, particularly in younger age groups, and can therefore be used as a practical and non-invasive tool for estimating livestock weight.
Co-Authors Adyanto Nessy Banamtuan Aloysius Marawali Apolonius Grad Suardi Kii, Mariana B.I. kirenius uly Kota, Ino Lawa, Alvrado Bire Listyadi, Rut Nalley, Wilmientje M Ni Made Paramita Setyani Petrus Kune Riwu, Agustinus Ridlof Riwu, Roni Markus Jami Simon Edison Mulik Thomas Mata Hine Uly, Kirenius W. Marlene Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Yohanes Paulus Martinus Bay Zulfa, Yanna Nailla