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Journal : METAMORFOSA Journal of Biological Sciences

OPTIMALISASI MEDIA ORGANIK UNTUK PERBANYAKAN ANGGREK HITAM (Coelogyne pandurata Lindl.) SECARA IN VITRO Ni Kadek Dwipayani Lestari; Ni Wayan Deswiniyanti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p13

Abstract

Populasi anggrek hitam (Coeloegyne pandurata Lindl.) terancam punah karena akibat eksploitasi yang berlebihan. Masalah tersebut dapat diatasi dengan teknik perbanyakan anggrek melalui kultur in vitro, namun harga media kultur jaringan relatif mahal. Tujuan penelitian ini adalah untuk memperoleh informasi tentang respon pertumbuhan benih anggrek hitam pada media kultur bahan organik dan modifikasinya, sehingga dapat digunakan sebagai media alternatif untuk pertumbuhan anggrek hitam yang optimal. Rancangan penelitian menggunakan Rancangan Acak Lengkap dengan 3 perlakuan yaitu media organik 1 (kentang), 2 (pisang ambon) dan 3(ubi jalar) masing-masing 10 ulangan. Data dianalisis secara statistik menggunakan Analisis Varians dan Uji Jarak Berganda Duncan pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa media organik kentang dan pisang ambon memiliki waktu tumbuh paling cepat dan jumlah tunas paling banyak dibandingkan media organik ubi jalar. Secara statistik kontaminasi tidak dipengaruhi secara signifikan oleh media, jadi dalam hal ini media paling baik/optimum adalah kentang dan pisang ambon, yang berbeda nyata dengan ubi jalar pada waktu tumbuh dan jumlah tunas.
PERSILANGAN INTERSPESIFIK ANGGREK HITAM (Coelogyne pandurata) DENGAN ANGGREK MUTIARA (Coelogyne asperata) Ni Wayan Deswiniyanti; Ni Kadek Dwipayani Lestari
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p15

Abstract

Black orchid (Coelogyne pandurata) and Pearl orchid (Coelogyne asperata) are endemic orchid from Kalimantan forest, shape and color suitable as ornamental flower. Many people collected the orchids from the wild for commercial purpose but not much effort on their propagation. To prevent population decreased, propagation and production of new varities via interspesific hybridisation need to be done. An important step required in plant breeding is to obtain cross compatiblity. Cross compatibility of some orchids from Indonesia are not known, including compatibility of Coelogyne pandurata x Coelogyne asperata. Information on compatibility and fertility is very important to produce good quality seed as propagation material. In this research pollination was performed on orchid plants at 2 until 5 day after flowering and pollination was done in the morning. There are three replicates for each pollination.  Pollination was done with C. pandurata (black orchid) as male parent and C. asperata (pearl orchid) as female parent. The results showed that successful pollination was obtained from pollination at 2 days after flowering. Seed capsule was harvested at 3 months after pollination but not yet perfectly ripe. Seed germination employed in vitro method on Vacin & Went (VW) and VW with modification media. Seed from C. pandurata x C. asperata abled to germinate on VW media with addition of 0,1 mg/l auxin and 0.1 mg/l cytokinin and germinated 9 weeks after planting.
PERUBAHAN ANATOMI STOMATA DAUN LILI TRUMPET (Lilium longiflorum) SETELAH PEMAPARAN RADIASI SINAR X Ni Kadek Dwipayani Lestari; Ida Ayu Astarini; I.G.M Oka Nurjaya
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lily (L. longiflorum) is one of 280 genus of liliaceae family. Variation on flower colour, growth habit, flowering period (shelf life) is very limited in Indonesia, so that plant breeding techniques are required to obtain a wide variety of plants. Physical mutation, radiation with X rays were employed in this study. The aim of this study is to find out the effect of X-ray exposure on lily’s leaves stomata anatomy. Lily seeds were grown in vitro and seedlings were exposed to X ray. This study employ CRD (completely randomized design) with four dose exposure (0 krad, 5 krad, 7,5 krad dan 10 krad) and 10 replicates each dose. Results show that to X ray causing reduction in stomata’s length, stomata amount, index, density of stomata that occurs randomly, compared to control.