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Exploring Potential Aquaculture-Immunostimulant-Peptides Derived from Chlorella sorokiniana Safitri, Nur Maulida; Violando, Wiga Alif; Suhermanto, Achmad; Rizkiah, Riza; Mukhaimin, Iman; Ramli, Taufik Hadi; Puspitasari, Asthervina Widyastami; Zummah, Atiqoh
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7585

Abstract

Chlorella sorokiniana is a microalgae with an outstanding nutritional profile and numerous therapeutic substances that can be used as an immunostimulant, including in aquaculture. This research aimed to investigate and characterize peptides isolated from C. sorokiniana protein using TCA digestion and hydrolyzed enzymatically with trypsin. Peptides were then subsequently identified using Tandem LC-MS/MS and Mascot Distiller. Results showed that the percentage of pure protein yield following TCA digestion was 54.66%, and 12 peptides with lengths ranging from 7 to 23 sequences were discovered after trypsin digestion. These peptides originated from various enzymes and chloroplast proteins, including protein synthesis elongation factor TU, photosystem I iron-sulfur center, photosystem II 43 kDa, Ycf4, ATP-dependent zinc metalloprotease FtsH homolog, nitrate reductase, chloroplastic glucose-6-phospate dehydrogenase, and ATP synthase CF1 alpha chain. These findings demonstrated that C. sorokiniana might serve as a source of immunostimulant peptides and proteins, particularly for aquaculture biota.
Analisis Potensi Antioksidan Rumput Laut (Kappaphycus alvarezii dan Sargassum sp.) Sebagai Produk Body Lotion Kamelia Rosa; Wiga Alif Violando
Jurnal Kelautan Vol 18, No 3: Desember (2025)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v18i3.28551

Abstract

ABSTRAKRumput laut kaya akan senyawa bioaktif, terutama senyawa fenol dan turunannya yang berfungsi sebagai mekanisme pertahanan terhadap lingkungan ekstrim. Senyawa bioaktif ini memiliki aktivitas antioksidan yang ditemukan dalam rumput laut. Antioksidan dalam kosmetik berfungsi untuk mencegah oksidasi yang dapat merusak formulasi kosmetik dan menangkal efek buruk radikal bebas yang dapat menyebabkan kerusakan kulit seperti kemerahan, kekeringan, dan pecah-pecah. Tujuan penelitian ini adalah menganalisis kandungan antioksidan dalam body lotion berbahan dasar rumput laut (Kappaphycus alvarezii) dan (Sargassum sp.) menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl), dengan pengukuran yang dilakukan menggunakan spektrofotometer pada panjang gelombang 517 nm. Hasil penelitian menunjukkan bahwa body lotion berbahan dasar rumput laut (Kappaphycus alvarezii) dan (Sargassum sp.) mengandung antioksidan, yang ditunjukkan oleh perubahan warna dari ungu menjadi kuning pucat setelah didiamkan selama 30 menit. Hasil uji aktivitas antioksidan menunjukkan bahwa nilai IC₅₀ formulasi terbaik (F3) sebesar 36,36 ppm, yang termasuk kategori aktivitas antioksidan sangat kuat. Sebagai pembanding, nilai IC₅₀ F0 sebesar 24.237,50 ppm, F1 sebesar 49,80 ppm, dan F2 sebesar 49,75 ppm yang menunjukkan bahwa body lotion berbahan dasar rumput laut (Kappaphycus alvarezii) dan (Sargassum sp.) memiliki aktivitas antioksidan yang sangat kuat. Temuan ini memiliki signifikansi praktis bagi industri kosmetik sebagai alternatif bahan antioksidan alami yang ramah lingkungan, sekaligus berpotensi meningkatkan nilai tambah ekonomi masyarakat pesisir melalui pemanfaatan rumput laut lokal Kata kunci: Rumput laut, Antioksidan, body lotionABSTRACTSeaweed is rich in bioactive compounds, particularly phenolic compounds and their derivatives, which serve as defense mechanisms against extreme environments. These bioactive compounds possess antioxidant activity found in seaweed. Antioxidants in cosmetics function to prevent oxidation that can damage cosmetic formulations and counteract the adverse effects of free radicals that can cause skin damage such as redness, dryness, and cracking. The aim of this study is to analyze the antioxidant content in seaweed-based body lotion (Kappaphycus alvarezii) and (Sargassum sp.) using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with measurements conducted using a spectrophotometer at a wavelength of 517 nm. The research results show that seaweed-based body lotion (Kappaphycus alvarezii) and (Sargassum sp.) contain antioxidants, as indicated by a color change from purple to pale yellow after being allowed to stand for 30 minutes. The antioxidant activity test showed that the IC₅₀ value of the best formulation (F3) was 36.36 ppm, which falls into the category of very strong antioxidant activity. For comparison, the IC₅₀ values of F0, F1, and F2 were 24,237.50 ppm, 49.80 ppm, and 49.75 ppm, respectively, indicating that the seaweed-based body lotion (Kappaphycus alvarezii and Sargassum sp.) possesses very strong antioxidant activity. This finding has practical significance for the cosmetics industry as an eco-friendly alternative source of natural antioxidants, while also offering the potential to enhance the economic value of coastal communities through the utilization of local seaweed. Keywords: Seaweed, Antioxidants, body lotion
A community-based participatory model for integrating lobster aquaculture into coastal tourism livelihoods Perdanawati, Rizqi Abdi; Mauludiyah; Nugraha, Dimas Rizal Wahyu; Hadi, Moch Irfan; Violando, Wiga Alif
Journal of Marine Resources and Coastal Management Vol. 6 No. 2 (2025)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v6i2.2500

Abstract

Bangsring Village, Banyuwangi Regency, Indonesia is a coastal tourism area with high ecological and economic potential; however, tourist visitation has declined significantly since 2020, highlighting the need for locally grounded and resilient livelihood innovations. This study aims to evaluate the feasibility and community relevance of lobster aquaculture as a tourism-supporting livelihood strategy using a Community-Based Participatory Research (CBPR) framework. The CBPR process was implemented through collaborative problem identification, participatory planning, joint environmental assessment, and community-led implementation involving the Pesona Bahari Community Group. Water quality parameters and site characteristics were jointly analyzed to inform technology selection. Based on these findings, a circular bottom (base) net-cage system was selected due to its suitability for moderate current conditions, benthic substrates, and its ability to mimic the natural habitat of lobsters. Program implementation included cage construction and deployment, seed stocking, daily maintenance using natural feed, and harvesting after approximately 100 days of rearing. The outcomes indicate increased community capacity in environmentally responsible lobster aquaculture practices and successful production of marketable-sized lobsters under local conditions. Beyond economic diversification, lobster farming activities also function as an educational and experiential attraction, strengthening the integration between aquaculture and coastal tourism. This study contributes a context-specific CBPR-based model for integrating small-scale lobster aquaculture with coastal tourism development. The approach demonstrates practical applicability and potential replicability for other coastal communities seeking sustainable livelihood diversification without compromising ecosystem integrity.
Pengaruh Sistem Budidaya Udang Vannamei pada Kolam Indoor dan Outdoor di IBL Boncong Agustin, Kurniasari Dwi; Almia, Bella Putri; Maisaroh, Dian Sari; Perdanawati, Rizqi Abdi; Putri, Dwi Ramdyana Rizqiana; Violando, Wiga Alif
Jurnal Pasir Laut Vol 10, No 1 (2026): Februari
Publisher : Master Program of Aquatic Resources Management, Department of Aquatic Resources, Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jpl.2026.81089

Abstract

Penelitian ini bertujuan untuk menganalisis pengaruh penerapan sistem budidaya outdoor dan indoor terhadap performa budidaya udang vannamei (Litopenaeus vannamei). Pengamatan dilakukan selama 90 hari atau satu siklus pemeliharaan dengan menggunakan metode observasi langsung, wawancara, serta partisipasi aktif dalam seluruh rangkaian kegiatan budidaya. Parameter yang diamati meliputi kualitas air, yang terdiri atas suhu, salinitas, pH, oksigen terlarut, dan kecerahan perairan. Selain itu, parameter pertumbuhan udang juga diamati, meliputi Average Body Weight (ABW), Average Daily Growth (ADG), Survival Rate (SR), serta Feed Conversion Ratio (FCR) sebagai indikator efisiensi pemanfaatan pakan. Hasil penelitian menunjukkan bahwa sistem budidaya outdoor memiliki kondisi suhu dan salinitas yang lebih mendekati kisaran optimal bagi pertumbuhan udang vannamei. Kondisi tersebut mampu mendukung laju pertumbuhan yang lebih cepat, yang ditunjukkan oleh nilai ABW akhir sebesar 26,35 g dan nilai ADG tertinggi mencapai 0,78 g/hari. Sebaliknya, sistem budidaya indoor menunjukkan laju pertumbuhan yang relatif lebih lambat, namun memiliki tingkat kelangsungan hidup yang lebih tinggi, yaitu sebesar 95%, dibandingkan dengan sistem outdoor yang mencapai 90%. Selain itu, nilai Feed Conversion Ratio (FCR) pada sistem indoor menunjukkan tingkat efisiensi pakan yang lebih baik dibandingkan sistem outdoor. Hal ini mengindikasikan bahwa kondisi lingkungan yang lebih terkendali pada sistem indoor mampu mendukung pemanfaatan pakan secara lebih optimal. Secara keseluruhan, sistem budidaya outdoor lebih unggul dalam meningkatkan pertumbuhan biomassa udang, sedangkan sistem budidaya indoor lebih efektif dalam menjaga tingkat kelangsungan hidup dan stabilitas lingkungan.