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All Journal Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Research Journal of Life Science Abjadia, International Journal of Education Jurnal Biologi Tropis Biota Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Perikanan Pantura SIYAR (Social Issues by Academic Researhers) Journal Engagement: Jurnal Pengabdian Kepada Masyarakat
Violando, Wiga Alif
UIN Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Exploring Potential Aquaculture-Immunostimulant-Peptides Derived from Chlorella sorokiniana Safitri, Nur Maulida; Violando, Wiga Alif; Suhermanto, Achmad; Rizkiah, Riza; Mukhaimin, Iman; Ramli, Taufik Hadi; Puspitasari, Asthervina Widyastami; Zummah, Atiqoh
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7585

Abstract

Chlorella sorokiniana is a microalgae with an outstanding nutritional profile and numerous therapeutic substances that can be used as an immunostimulant, including in aquaculture. This research aimed to investigate and characterize peptides isolated from C. sorokiniana protein using TCA digestion and hydrolyzed enzymatically with trypsin. Peptides were then subsequently identified using Tandem LC-MS/MS and Mascot Distiller. Results showed that the percentage of pure protein yield following TCA digestion was 54.66%, and 12 peptides with lengths ranging from 7 to 23 sequences were discovered after trypsin digestion. These peptides originated from various enzymes and chloroplast proteins, including protein synthesis elongation factor TU, photosystem I iron-sulfur center, photosystem II 43 kDa, Ycf4, ATP-dependent zinc metalloprotease FtsH homolog, nitrate reductase, chloroplastic glucose-6-phospate dehydrogenase, and ATP synthase CF1 alpha chain. These findings demonstrated that C. sorokiniana might serve as a source of immunostimulant peptides and proteins, particularly for aquaculture biota.
Analisis Potensi Antioksidan Rumput Laut (Kappaphycus alvarezii dan Sargassum sp.) Sebagai Produk Body Lotion Rosa, Kamelia; Violando, Wiga Alif
Jurnal Kelautan Vol 18, No 3: Desember (2025)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v18i3.28551

Abstract

ABSTRAKRumput laut kaya akan senyawa bioaktif, terutama senyawa fenol dan turunannya yang berfungsi sebagai mekanisme pertahanan terhadap lingkungan ekstrim. Senyawa bioaktif ini memiliki aktivitas antioksidan yang ditemukan dalam rumput laut. Antioksidan dalam kosmetik berfungsi untuk mencegah oksidasi yang dapat merusak formulasi kosmetik dan menangkal efek buruk radikal bebas yang dapat menyebabkan kerusakan kulit seperti kemerahan, kekeringan, dan pecah-pecah. Tujuan penelitian ini adalah menganalisis kandungan antioksidan dalam body lotion berbahan dasar rumput laut (Kappaphycus alvarezii) dan (Sargassum sp.) menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl), dengan pengukuran yang dilakukan menggunakan spektrofotometer pada panjang gelombang 517 nm. Hasil penelitian menunjukkan bahwa body lotion berbahan dasar rumput laut (Kappaphycus alvarezii) dan (Sargassum sp.) mengandung antioksidan, yang ditunjukkan oleh perubahan warna dari ungu menjadi kuning pucat setelah didiamkan selama 30 menit. Hasil uji aktivitas antioksidan menunjukkan bahwa nilai IC₅₀ formulasi terbaik (F3) sebesar 36,36 ppm, yang termasuk kategori aktivitas antioksidan sangat kuat. Sebagai pembanding, nilai IC₅₀ F0 sebesar 24.237,50 ppm, F1 sebesar 49,80 ppm, dan F2 sebesar 49,75 ppm yang menunjukkan bahwa body lotion berbahan dasar rumput laut (Kappaphycus alvarezii) dan (Sargassum sp.) memiliki aktivitas antioksidan yang sangat kuat. Temuan ini memiliki signifikansi praktis bagi industri kosmetik sebagai alternatif bahan antioksidan alami yang ramah lingkungan, sekaligus berpotensi meningkatkan nilai tambah ekonomi masyarakat pesisir melalui pemanfaatan rumput laut lokal Kata kunci: Rumput laut, Antioksidan, body lotionABSTRACTSeaweed is rich in bioactive compounds, particularly phenolic compounds and their derivatives, which serve as defense mechanisms against extreme environments. These bioactive compounds possess antioxidant activity found in seaweed. Antioxidants in cosmetics function to prevent oxidation that can damage cosmetic formulations and counteract the adverse effects of free radicals that can cause skin damage such as redness, dryness, and cracking. The aim of this study is to analyze the antioxidant content in seaweed-based body lotion (Kappaphycus alvarezii) and (Sargassum sp.) using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with measurements conducted using a spectrophotometer at a wavelength of 517 nm. The research results show that seaweed-based body lotion (Kappaphycus alvarezii) and (Sargassum sp.) contain antioxidants, as indicated by a color change from purple to pale yellow after being allowed to stand for 30 minutes. The antioxidant activity test showed that the IC₅₀ value of the best formulation (F3) was 36.36 ppm, which falls into the category of very strong antioxidant activity. For comparison, the IC₅₀ values of F0, F1, and F2 were 24,237.50 ppm, 49.80 ppm, and 49.75 ppm, respectively, indicating that the seaweed-based body lotion (Kappaphycus alvarezii and Sargassum sp.) possesses very strong antioxidant activity. This finding has practical significance for the cosmetics industry as an eco-friendly alternative source of natural antioxidants, while also offering the potential to enhance the economic value of coastal communities through the utilization of local seaweed. Keywords: Seaweed, Antioxidants, body lotion
Co-Authors Abdul Azis Agus Solikin Albarkah, M. Rafly Andi Rahmad Rahim Asri Sawiji Atiqoh Zummah Chih-Hsuan Tseng Dian Tugu Warsito Taufiq Esti Tyastirin Faidlul Qodir Fatih, M. Fachrul Febryanti Nur Maulidia Gamal Kusuma Zamahsari Idris Idris M. Chusnan Ma'arif Maisaroh, Dian Sari Maulidia, Febryanti Nur Mauludiyah Mauludiyah Ma’arif, M. Chusnan Moch. Irfan Hadi Mohammad Wildhan Firmansyah Zam Zamy Muhammad Rizky Adipratama Muhid , Abdul Mukhaimin, Iman Nugraha, Dimas Putra Wahyu Nur Maulida Safitri Nurliah Nusa, Raras Widya Ofri Johan Ofri Johan Paryono Puji Maryono Puspitasari, Asthervina Widyastami Putikadyanto, Agus Purnomo Ahmad Putra, Riswanda Rangga Ramli, Taufik Hadi Rizkiah, Riza Robayanto Robayanto Rosa, Kamelia Sadikin Amir Saiful Bahri Saou-Lien Wong Saptono Waspodo Selobing Purna Agung Indarto Sri Andayani Suhermanto, Achmad Zam Zamy, Mohammad Wildhan Firmansyah