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The potency of green grass jelly extract (Premna oblongifolia Merr) as antihyperlipidemia towards aorta histopathology representation of rat (Rattus norvegicus) induced with high fatty diet (HFD) Winthoko, Eka Nora Vitaloka Aprilia Putri; Roosdiana, Anna; Pratama, Dyah Ayu Oktaviani A; Nugraha, Jusak; Purwanta, Marijam; Rifaâ€™I, Muh Husni; Rendy, Achmad Nur
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.174

Green grass jelly (Premna oblongifolia merr) is a plant containing fiber and chlorophyll which can lower cholesterol and triglyceride levels. This study have aims to investigate the potency of green grass jelly extract (Premna oblongifolia Merr) to prevent hyperlipidemia. The animal mode used for this study is male Rattus norvegicus, Wistar strain, the age of 8 weeks, and weight of 200 g which is divided into 5 groups of treatment namely group Kn (negative control), Kp (positive control), Kp1, Kp2, and Kp3 induced with HFD and green grass jelly extract at a dose of 5.27 g/ kg BW/ daily, 8.43 g/ kg BW/ daily, 9.37 g/ kg BW/ daily. The data of infiltrating fatty cells and macrophage on aorta histopathology was analyzed by description. This research showed that the treatment of green grass jelly extract (Premna oblongifolia Merr) to animals of hyperlipidemia model reduced infiltration fatty cells and macrophage. The conclusion of this study was the green grass jelly extract was able to prevent an increase of fatty cells and macrophage infiltration of rat (Rattus noervegicus) induced with HFD on dose 9.37 g/ kg/ BW/ daily.

TUBERKULOSIS (TB) ADALAH SUATU P GAMBARAN INDURASI DAN EKSPRESI CD163 SEBAGAI MARKER AKTIVASI MAKROFAG M2 PADA TIKUS RATTUS NORVEGICUS YANG DIIJEKSI DENGAN PURIFIED PROTEIN DERIVATE (PPD) lestari, veni ayu
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1) (inpress)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.179

Abstrak               Tuberkulosis (TB) adalah suatu penyakit infeksi menular yang disebabkan oleh Mycobacterium tuberculosis (MTB), yang dapat menyerang beberapa organ terutama paru-paru. Untuk mengidentifikasi kasus TB mulai dikembangkan Tuberculin Skin Test (TST). Tes kulit tuberkulin, yang melibatkan pemantauan reaksi kekebalan terhadap suntikan Purified Protein Derivative (PPD). Tujuan penelitian ini adalah untuk mengamati indurasi dan ekspresi CD163 sebagai marker aktivasi makrofag M2 pada tikus. Metode penelitian ini bersifat eksperimental yang akan disajikan dalam bentuk gambar secara deskriptif. Sampel penelitian ini adalah tikus (Rattus norvegicus) dengan jumlah 7 ekor yang dibagi menjadi 2 yaitu 1 ekor untuk kontrol dan 6 ekor untuk perlakukan. Penelitian ini dilakukan di Laboratorium Biokimia Universitas Airlangga pada bulan April 2019. Hasil penelitian ini adalah pada tikus perlakuan terjadi indurasi setelah 42 dan 72 jam setelah diinjeksi Purified Protein Derivative (PPD) dengan hasil ? 5 mm yang dapat diartikan sebagai indurasi positif sehingga dapat disimpulkan makrofag teraktivasi dan hasil pemeriksaan imunohistokimia pada jaringan kulit tikus dengan mikroskop didapatkan hasil dari 6 sampel perlakuan 3 sampel diantaranya menunjukkan adanya ekspresi CD163 ditandai dengan adanya sel radang dan granuloma. Maka dapat disimpulkan bahwa PPD yang diinjeksikan pada tikus dapat mengaktifkan sel radang dan granuloma serta mengekpresikan CD163 pada jaringan kulit tikus.     Kata Kunci : PPD, Tikus, CD163

The potential of traditional balinese spices against the growth of Salmonella sp in vitro Jirna, I Nyoman; Sudarmanto, I Gede; Kurniawan, Surya Bayu; Ratih, Gusti Ayu Made; Rasyid, Burhannuddin
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.200

Prevention by utilizing medicinal plants can be used as an alternative treatment. This study aims to identify active antimicrobial substances and test the antimicrobial potential of traditional Balinese spices, namely Basa Selem, Basa Gede, Basa Wangen, Basa Bawang Jahe, and Basa Rajang against Salmonella sp bacteria in vitro. The True-experimental method with Posttest only-control design was used in this study, by intervening in the treatment group as well as the presence of positive and negative controls. The maceration method with 96% EtOH solvent was used to extract active substances and identify the levels of antimicrobial active substances. The TLC Spectrophotodensitometer instrument and the diffusion method (discs) were used to test antimicrobial potential. Data analysis was performed using the one-way ANOVA test. All five samples showed flavonoids, alkaloids, tannins, and phenols in qualitative tests. Based on quantitative test results of five samples, the highest compound content obtained in Basa Wangen (6.66 mg/ml of tannins), Basa Gede (3.74 mg/ml of flavonoids), Basa Bawang Jahe (2.49 mg/ml of tannins), Basa Selem (2.87 mg/ml of tannin), and Basa Rajang (6.96 mg/ml of flavonoids). There are differences in the antimicrobial potential of various types of traditional Balinese spices against the growth of Salmonella sp in vitro (sig = 0.037). The traditional Balinese spices have the antimicrobial potential of the intermediate category with a range of inhibition (16-20 mm) based on the NCCLS standard.

Second allogeneic stem cell transplantation in acute leukemia patients: single-centre experience BakÄ±rtaÅŸ, Mehmet; YiÄŸenoÄŸlu, TuÄŸÃ§e Nur; BaÅŸcÄ±, Semih; Uncu Ulu, Bahar; Ã–zcan, NurgÃ¼l; Ä°skender, Dicle; Dal, Mehmet Sinan; KÄ±zÄ±l Ã‡akar, Merih; AltuntaÅŸ, Fevzi
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.208

Acute leukaemia patients who relapse after the first allogeneic stem cell transplantation (Allo-SCT) have a poor prognosis. Participating in clinical trials is the best option for these patients. If patients cannot participate in clinical trials, as the treatment options are limited, the second allo-SCT constitutes the potential curative treatment option. The data of acute leukaemia patients who underwent second allo-SCT because of relapsed/refractory disease after the first allo-SCT at our centre between December 2009 and February 2019 were analyzed retrospectively. Three hundred nineteen acute leukaemia patients were performed allo-SCT at our centre. 20 of these 319 acute leukaemia patients relapsed after first allo-SCT and underwent second allo-SCT. 10 AML patients and 10 ALL patients were included in the study. After second allo-SCT overall survival (OS) was 26.1Â±10.8 weeks, and progression-free survival (PFS) was 19.9Â±8.6 weeks. If the patients cannot participate in clinical trials, second allo-SCT should be considered for patients with late (â‰¥12 months) relapses after the first allo-SCT. If possible, haploidentical donors should be selected for second allo-SCT and patients should be in complete remission before the transplant.

Does platelet/ lymphocyte ratio a predictor of CD34+ peripheral blood stem cell yield in the healthy donors mobilized with GCSF? Gokgoz, Zafer
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.209

This research aimed to determine whether there is a correlation between platelet/lymphocyte ratio the number of collected CD34+ stem cells. This retrospective study included 94 adult related stem cell donors who were healthy and volunteer by screening their files between the years 2016 and 2018. All donors were mobilized using 2.5 mcg/kg lenograstim or filgrastim and underwent peripheral stem cell apheresis. Complete blood counts were tested at baseline before G-CSF administration (preâ€“G-CSF), and before PBSC collection after mobilization with G-CSF administration. The patients were divided into two groups as aged below and over 50 years old. From these comparative data, only BMI value of the group aged below 50 years was statistically significantly lower than the other group, whereas no statistically significant difference was found between the groups in terms of other parameters. The numbers of the collected CD34+ stem cells of both age groups were similar, and no significant difference was found. The values of platelet/lymphocyte ratio, early measurement of CD34+ stem cells and the amount of the collected CD34+ stem cells of both groups at the first and second days of the procedure were found similar. This research show that a high platelet count and consequently, a high platelet/lymphocyte ratio may be correlated with the number of collected CD34+ stem cells but our hypotheses revealed insignificant outcomes.

Modulation of severe acute respiratory syndrome coronavirus (SARS-CoV-2) in receptor, innate immunity and drug antiviral candidate Tarigan, Indra Lasmana; Arum, Kartika
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The Coronavirus disease-19 (COVID-19) is a contagious acute respiratory infectious disease caused by SARS-CoV-2 as a global pandemic in 2020. This disease most spreads and causes some severe cases, even death in the world. The primary purpose of this review discusses the recent article that was published regarding COVID-19 genomic modulation, the mechanism of innate immunity, and the screening of anti-viral drug candidates, for treating COVID-19 patients. This review used the latest paper regarding COVID-19 with 63 journals with high impact factors such as Nature, The Lancet, Cells, International Journal of Biological Sciences, Mol Biol Methods. Journal of Microbiology, Immunology, and Infection, Nat. Rev. Microbiol, and other international journals indexed by Scopus, Elsevier, and Springer through in vivo and in vitro studies. The genomic of SARS-CoV-2 consist high similarly to coronaviruses family, albeit possessing a different pathway even has higher affinity, due to changing some nitrogen bases are supposed to have a significant effect on its pneumonia. Herein, we report review article an update on the recent literature of the COVID-19 modulation genome, mechanism of innate immunity, and medical literature. Moreover, we report anti-viral drugs that have been developed from synthetic drugs and medicinal compounds from plants. Several studies have been re-analyzed using in vitro, in vivo, and modelling using bioinformatics tools.

Perspective of molecular immune response of SARS-COV-2 infection Rohmah, Martina Kurnia; Rahman Nurdianto, Arif
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

COVID-19 is a type of Pneumonia caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). When COVID-19 arise in Wuhan China and rapidly spread throughout to the World, we need to learn how pathogenesis and immune responses occur in the bodies in more detail. COVID-19 is the third Severe Respiratory Disease outbreak caused by the Coronavirus in the past two decades after Severe Acute Respiratory Syndrome (SARS) in the 2002 and Middle East Respiratory Syndrome (MERS) in 2012. The Articles from PUBMED and Research Gate were searched for studies on the immune response of COVID-19 infection by SARS-CoV-2. SARS-CoV-2 increases the number of neutrophils, suppresses IFN, increases the activity of Th1/Th17, B cells, CD8+ and CD4+, and causes cytokine storms especially pro-inflammatory cytokines which can increase respiration disorders and multi-organ damage. This review tries to explain about pathogenesis and immune responses of COVID-19 to provide a reference in designing the appropriate immune intervention for treatment and therapeutic such as drug or vaccine based on the recent research progress SARS-CoV-2 and previous studies about SARS CoV and MERS CoV.

Herd immunity and COVID-19 in Indonesia Faizal, Imam Agus; Ariska Nugrahani, Nur
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Herd immunity or herd effect is a phenomenon that occurs in groups of people who are resistant to disease. The purpose of conducting this research is to predict the number of cumulative cases of COVID-19 in Indonesia. Covid-19 cases in Indonesia on April 6, 2020, were 2235 cases spread in 34 Provinces. As many as 2491 cases in Indonesia, there were 192 patients recovered (including those treated, so they have natural antibodies in the end) while the total who died was 209 people. It is assumed that around 13% of the total cases have natural antibodies. This is also the case with SARS-CoV-2 and may explain why some individuals (perhaps those most recently able to recover from seasonal coronavirus infections) have asymptomatic infections. Finally, the theoretical concept of increasing herd immunity in pandemic and epidemic cases in Indonesia which aims to control COVID-19 still needs to be reviewed because it is seen from the mortality data that CFR COVID-19 is predicted to be around 8.39% of the population in Indonesia where the risk of death still available. The best alternative is to do a healthy lifestyle, social distancing, and waiting for the vaccine to be found.

Investigation of revers-transcriptase polymerase chain reaction values of patients with COVID-19 findings in lung computed tomography results Osman, OzÃ¼dogru; Bolatli, Gunes; Tas, Fatih
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

It turned out that the cause of pneumonia cases that occurred in China was due to SARS-CoV-2. The aim is to compare chest computer tomography and Revers-Transcriptase Polymerase Chain Reaction methods used in the diagnosis of COVID-19 disease with each other and to evaluate this disease with risk factors. The study was carried out on 66 patients. Epidemiological history, clinical symptoms, chest CT and RT-PCR results of the cases were examined. RT-PCR results of 1, 4, and 7 days were evaluated for each case with positive chest CT results. Thirty-seven of them were found to be positive on day 1, 5 of them were found to be positive on day 4, and 2 of them were found to be positive on day seven from 52 patients whose RT-PCR results were examined. In the remaining 8 cases, no positive findings were found. The most common findings are; cough (78.8%), fever (55.8%), and shortness of breath (28.8%). It was observed that 51.9% of the cases had chronic disease history and 50% of the patients using cigarettes had bilateral lung involvement in their CT results. Seven cases received intensive care support, 3 cases were intubated. Two of the intubated cases were exitus (3,8%). The positive results of RT-PCR were found to be negative in most of the cases which have positive chest CT; suggests that chest CT is more reliable in making a diagnosis. Therefore, evaluating chest CT results with RT-PCR can be an appropriate alternative approach in the diagnosis and treatment of disease. However, in order to be fully diagnosed, the patient's history, chronic diseases, age, symptoms, imaging, blood, and test findings must all be considered as a whole.

Immunobioinformatics analysis and phylogenetic tree construction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Indonesia: spike glycoprotein gene Ansori, Arif Nur Muhammad; Kharisma, Viol Dhea; Antonius, Yulanda; Tacharina, Martia Rani; Rantam, Fedik Abdul
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has spread worldwide and as a result, the World Health Organization (WHO) declared it a pandemic. At present, there are no approved vaccines against SARS-CoV-2. Therefore, the aim of this study was to predict epitope-based vaccines using bioinformatics approaches and phylogenetic tree construction of SARS-CoV-2 against the backdrop of the COVID-19 pandemic. In this study, we employed 27 isolates of SARS-CoV-2 spike glycoprotein genes retrieved from GenBankÂ® (National Center for Biotechnology Information, USA) and the GISAID EpiCoVâ„¢ Database (Germany). We analyzed the candidate epitopes using the Immune Epitope Database and Analysis Resource. Furthermore, we performed a protective antigen prediction with VaxiJen 2.0. Data for B-cell epitope prediction, protective antigen prediction, and the underlying phylogenetic tree of SARS-CoV-2 were obtained in this research. Therefore, these data could be used to design an epitope-based vaccine against SARS-CoV-2. However, the advanced study is recommended for confirmation (in vitro and in vivo).

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Journal Mail Official
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Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA