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Optimalisasi Ekstraksi DNA Dan PCR Untuk Identifikasi Molekuler Pada 4 Jenis Karang Lunak Berbeda Aradea Bujana Kusuma
JURNAL ENGGANO Vol. 7 No. 2 (2022)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.7.2.175-182

Karang lunak merupakan salah satu penyusun komunitas karang yang berguna bagi ekosistem terumbu karang. Keberadaanya di ekosistem terumbu karang sangat penting bagi proses rantai makanan di lautan. Sulitnya identifikasi morfologi karang ini disebabkan variasi sklerit yang beragam. Oleh karena itu penting adanya identifikasi molekuler sebagai penunjang data taksonomi karang ini. Akan tetapi, dalam proses identifikasi molekuler karang ini masih banyak hambatan yang terjadi dalam proses ekstraksi maupun PCR nya. Hal ini dimungkinkan karena adanya kalsium karbonat yang tersusun di seluruh tubuh karang ini. Oleh karena itu, penelitian ini dilakukan untuk mendapatkan proses ekstraksi DNA dan PCR yang optimal dalam proses identifikasi secara molekuler. Proses ekstraksi dilakukan dengan menggunakan Kit Ekstraksi dan PCR dengan menggunakan KIT PCR. Hasil ekstraksi menunjukan bahwa Xenia sp memiliki konsentrasi DNA yang tertinggi dengan nilai 53.90 (ng/ul) dan terendah pada jenis Sarcophyton trocheliophorum sebesar 26.70 (ng/ul). Optimalisasi PCR menghasilkan hasil yang baik pada spesies Xenia sp., dan Lobophytum pauciflorumsedangkan pada sampel jenis Sarcophyton trocheliophorum pita DNA menunjukan hasil yang smear. Keywords: Ekstraksi, Identifikasi, Karang Lunak, Molekuler, PCR

Inventarisasi Ikan Tembakul (Oxudercinae) dari Lingkungan Intertidal Kabupaten Bengkalis Juliana, Juliana; Mahatma, Radith; Muhammad, Ahmad
JURNAL ENGGANO Vol. 8 No. 2 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.8.2.165-171

Ikan tembakul (mudskipper) banyak dijumpai di lingkungan intertidal baik mangrove maupun pantai Kabupaten Bengkalis. Namun informasi mengenai jenis-jenis ikan tembakul di Kabupaten Bengkalis masih terbatas. Penelitian ini bertujuan untuk mengetahui jenis-jenis ikan tembakul yang ada di lingkungan intertidal Kabupaten Bengkalis. Pengambilan sampel ikan tembakul dilakukan pada Oktober 2020 dengan menggunakan tangguk, pancing atau langsung dengan tangan di Tanjung Leban dan Bukit Batu Laut. Hasil penelitian ini ditemukan lima spesies dari tiga genus ikan tembakul yang terdiri dari Boleophthalmus boddarti, Periophthalmodon schlosseri, Pn. septemradiatus, Periophthalmus chrysospilos dan P. variabilis.

Kesesuaian Wisata Snorkeling dan Diving di Gusung Perlang Perairan Perlang Bangka Tengah Robi Gunawan; Sudirman Adibrata; Andi Gustomi
JURNAL ENGGANO Vol. 8 No. 2 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.8.2.154-164

ABSTRAK Gusung Perlang is located in Perlang Village, Central Bangka Regency. Gusung Perlang has underwater potential, namely coral reef ecosystems that becomes a tourist attraction in which the unique shape, color, and diversity of the biota with high selling value. This study aims to determine the value of the tourism suitability index, the carrying capacity of the snorkeling, and diving tourism area in the east Gusung Perlang waters. Moreover, the process of sampling is carried out in August 2021 at Gusung Perlang, Central Bangka Regency. The method of this study uses purposive sampling. Coral reef data is collected using the UPT (Underwater Photo Transect) method, reef fish using the visual census method and the time swim method. In addition, the data analysis is based on the tourism suitability index (IKW) matrix. The results of the Snorkeling Tourism Suitability Index (IKW) show that the location is suitable for station 1 (78.95%), station 3 (78.95%) and station 5 (77.19%), the location is conditional status in station 2 (70.18%). ) and station 4 (70.18%). The results of the tourism suitability index (IKW) calculation for diving show that the location is suitable for station 3 (77.78%), the location is conditional status in station 1 (72.22%), station 2 (62.96%), station 4 (62.96). %) and station 5 (74.07%). Keywords: Suitability, Gusung Perlang, Snorkeling, Diving

Evaluasi Mutu Kualitas Air Kolam IPAL Tambak Udang Skala Rakyat Desa Kurau Barat, Kabupaten Bangka Tengah Notonegoro, Hartoyo
JURNAL ENGGANO Vol. 8 No. 2 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.8.2.172-180

Budidaya perikanan khususnya tambak udang merupakan salah satu andalan yang banyak dikembangkan dan dikelola oleh masyarakat karena memberikan kontribusi ekonomi melalui peningkatan pendapatan, baik sebagai produk utama maupun sampingan. Keberadaan tambak udang selain dapat menghasilkan tanaman berupa udang juga dapat menghasilkan limbah dari tambak udang yang berpotensi mencemari lingkungan perairan dan selanjutnya mempengaruhi kehidupan organisme perairan. Salah satu tambak udang yang memiliki IPAL di wilayah pesisir Indonesia terletak di Desa Kurau, Kecamatan Koba, Kabupaten Bangka Tengah, Provinsi Kepulauan Bangka Belitung. Berdasarkan hasil wawancara dengan kelompok masyarakat tambak skala kecil di Desa Kurau Barat, selama ini pengendalian kualitas air pada IPAL dan limbahnya hanya menggunakan ikan sebagai bioindikator pencemaran air. Belum adanya pengendalian air limbah tambak udang sesuai baku mutu, sehingga perlu dilakukan evaluasi kesesuaian baku mutu air. Penelitian ini bertujuan untuk menganalisis kualitas air IPAL tambak untuk tambak udang berdasarkan baku mutu effluent dari tambak udang dan menganalisis pencemaran IPAL tambak berdasarkan metode Storet. Tiga stasiun berbeda di lokasi penelitian ditentukan secara purposive sampling, dengan pertimbangan dapat mewakili kondisi lokasi penelitian. Hasil pengukuran kualitas air terdapat tiga parameter yang melebihi ambang batas baku mutu air menurut PP No.22 Tahun 2021 yaitu pH, BOD dan amoniak. Berdasarkan hasil perhitungan analisis STORET pada tambak IPAL tambak udang di Desa Kurau Barat sudah termasuk dalam kategori tercemar sedang.

Struktur Komunitas Mangrove di Desa Sungai Nibung Kabupaten Tulang Bawang Provinsi Lampung Kusuma, Anma Hari
JURNAL ENGGANO Vol. 8 No. 2 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.8.2.135-146

Mangrove merupakan tumbuhan khas yang hidup di pesisir tropis yang dipengaruhi oleh pasang surut dan mampu beradaptasi dengan salinitas. Mangrove banyak sekali memiliki fungsi ekologi dan ekonomi. Desa Sungai Nibung merupakan salah satu desa yang memiliki potensi mangrove. Saat ini keberadaan kawasan mangrove di lokasi tersebut sebagian besar telah mengalami alih fungsi untuk keperluan pertambakan dan penebangan liar. Penelitian ini bertujuan mengkaji struktur dan sebaran mangrove berdasarkan karakteristik lingkungan di Desa Sungai Nibung. Penelitian ini dilakukan pada bulan Agustus 2022. Lokasi penelitian di Desa Sungai Nibung, Kecamatan Dente Teladas, Kabupaten Tulang Bawang, Provinsi Lampung. Komposisi mangrove di Desa Sungai Nibung memiliki kategori pertumbuhan mangrove yang lengkap mulai dari pohon, anakan, dan semai. Kompisisi vegetasi mangrove yang ditemukan di Desa Sungai Nibung sebanyak 5 (lima) jenis, yaitu jenis Avicennia marina, Avicennia officinalis, Exoecaria agallocha, Bruguiera cylindrica dan Rhizophora apiculata. Kenaekaragaman jenis mangrove di lokasi penelitian ini cukup tergolong tinggi dengan kondisi fisika-kimia perairan yang medukung untuk pertumbuhan mangrove. Kata kunci : Desa Sungai Nibung, Parameter Fisika-Kimia, Vegetasi Mangrove

Komposisi Nutrisi Rumput Laut Coklat (Phaeophyta) dan Merah (Rhodophyta) Asal Perairan Teluk Sepang Kota Bengkulu herliany, vivien; Utami, Maya Angraini Fajar; Wilopo, Mukti Dono; Permatasari, Nurul
JURNAL ENGGANO Vol. 8 No. 2 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.8.2.147-153

Seaweeds have potential uses as functional foods. But until now, there is still few information about the nutritional composition of seaweeds, especially from Teluk Sepang Beach, Bengkulu City. This study was held to examine the chemical characteristics of brown and red seaweeds from Teluk Sepang Beach, Bengkulu City. This research was carried out in November 2021-March 2022. This research used descriptive exploratory method by analyzing the proximate composition of 8 species of seaweeds (4 species of brown seaweeds and 4 species of red seaweeds). Water, protein, crude fat, ash and crude fiber content refer to the AOAC (2016) procedure while carbohydrate content is calculated based on by difference. The chemical characteristics of seaweeds (water content, ash, crude protein, crude fiber, crude fat and carbohydrates content) varied from one species to another. But in general the proximate composition of all seaweeds species was still in the range of values found in seaweeds. The largest composition in seaweesd is carbohydrates (13.644% - 69.91±0.71%) and ash, which have an important role for humans nutrition and the food industry. The content of protein (0.97±0.33%. - 2.88±0.23%) and crude fat (0.19±00% - 4.12±0.32%) in seaweeds is relatively low so it can be used as low fat foods. Crude fiber in seaweeds(1.16±0.16% - 7.65±0.53%) showed that seaweeds can be used as functional foods.

Aspek Biologi Hiu (Rhizoprionodon taylori) Hasil Tangkapan di Perairan WPP 718 Indonesia Firdha Iresta Wardani
JURNAL ENGGANO Vol. 9 No. 1 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.9.1.36-43

Shark fishing activity is an economically important activity because sharks are the target fish for export purposes. Sharks are consumed by their fins dan flesh. The relatively high demand demand for shark exports with low reproduction characteristics makes sharks more vulnerable to population threats. In managing sharks fishery, biological aspect are supporting things as a reference for management actions. The study aims to describe the fishing characteristic of shark (Rhizoprionodon taylori), length to weight relationship, length distribution, and sex ratio. Sharks are more susceptible to population risks because of the relatively high demand for shark exports and their lack of reporting characteristics. Biology plays a supportive role in shark management by serving as a point of reference for management efforts. This study attempts to describe the length-to-weight relationship, length distribution, and sex ratio of the shark (Rhizoprionodon taylori) as they relate to fishing. The collection and capture of biological data took place between February and August of 2022. TPI Dermaga Barat Pelabuhan Perikanan Samedera Nizam Zachman (PPSNZ), Muara Baru, North Jakarta, is where the fish used for the fish samples were landed. Biology fish samples data were weighed,fork length and sex. At sizes ranging from 38.6 cm to 43 cm and a negative allometric development trend, the long shark (Rhizopodon taylori) predominates. 94% of the sampled fish were female, showing that female fish predominate in the catch. It will decrease fish stocks that can reproduce, which will result in lower recruitment in the waters, if the capture is consistently dominated by female fish. Keywords : Long Weight Relationship, Shark Catching, Sex Ratio, WPP 718

A Morfologi dan Genetik Ikan Sidat (Anguilla marmorata) di Sungai Prafi Kabupaten Manokwari Provinsi Papua Barat Bayu Pranata; Ida Lapadi; Fanny F.C. Simatauw; Andi Fajeriani Wyrasti; Mansyur, Fadhilah Iqra; Kusuma, Aradea Bujana
JURNAL ENGGANO Vol. 9 No. 1 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.9.1.11-23

Di perairan Indonesia ditemukan tujuh spesies ikan Sidat, yaitu A. marmorata, A. celebesensis, A. borneensis, A. bicolor bicolor, A. bicolor pacifica, A. interioris dan A. nebulosa. Ikan Sidat (Anguilla marmorata) adalah salah satu spesies yang memiliki nilai ekonomis tinggi dan telah menjadi komoditas tujuan eksport. Spesies ini banyak ditemukan di perairan Sungai di Indonesia termaksud di Sungai Prafi Kabupaten Manokwari, Papua Barat. Penelitian ini bertujuan untuk mengidentifikasi ikan Sidat dengan pendekatan morfologi dan genetik serta memahami hubungan genetic spesies A. marmorata. Metode BLAST di NCBI digunakan untuk mengidentifikasi sampel ikan Sidat. 31 Sampel penelitian teridentifikasi sebagai A. marmorata dengan nilai percent identity 100% dan query coverage 99%. A. marmorata yang ditangkap memiliki berat rata-rata sebesar 257.48 gr, panjang rata-rata yaitu 43.19 cm dan jumlah rata-rata ruas tulang ana dorsal sebanyak 18. A. marmorata yang berasal dari manokwari di rekonstruksi dengan dengan beberapa A. marmorata yang berasal dari berbagai negara. Hasil penelitian menunjukkan bahwa, A. marmorata dari manokwari berada pada satu klade yang sama dengan beberapa individu A. marmorata dari China, Brunei, Indonesia-Bali-Jawa Barat, Vietnam, Malaysia, Japang dan French Polynesia. Hal tersebut didukung oleh nilai jarak genetik antar populasi A. marmorata yang rendah yaitu 0%-0,4%. Rekonstruksi pohon filogenetik antar spesies Anguilla sp. menunjukkan bahwa A. marmorata berada pada clade yang sama dengan A. Nebulosa. Hal tersebut didukung oleh nilai jarak genetik yang rendah yaitu 3%. Sedangkan nilai jarak genetik tertinggi yaitu antar A. mossambica dan A. celebesensis (11,4%). Kata kunci : Anguilla marmorata, Genetik, Morfologi, Manokwari, Papua

Identifikasi Keanekaragaman Spesies Elasmobranchii Hasil Tangkapan Nelayan Lokal Yang Didaratkan di Taman Wisata Perairan Timur Pulau Bintan Zamalludin, Zamalludin; Karlina, Ita; Hikmat Nugraha, Aditya; Armanto, Tri; Yandri, Fahmi; Angraini, Rika; Koenawan, Chandra Joei
JURNAL ENGGANO Vol. 9 No. 1 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.9.1.24-35

Riau Islands Province has a larger water area than its land. This large water area then began to be managed with the establishment of a conservation area known as the Bintan Island Eastern Waters Tourism Park. The vast waters in the Riau Islands are thought to also have high diversity, one of which is Elasmobranchii species. The purpose of this study is to identify the species diversity and conservation status of Elasmobranchii from the catches landed by local fishermen in Bintan Island Eastern Waters Tourism Park. The method used in this study is a survey method, which is descriptive to determine the description of the results of the identification of species diversity and conservation status of Elasmobranchii. From the results of this study, 12 species and 8 families of 72 Elasmobranchii individuals were identified, with a diversity index value of H' = 2.21 (medium), uniformity index E = 0.89 (very good), and dominance index C = 0.14 (low) or no dominating species. From the conservation status analysis, it was found that 42% or 5 out of 12 Elasmobranchii species were categorized as Vulnerebel, 25% (3 species) were categorized as endangered and 8% (1 species) were categorized as critically endangered, 75% of Elasmobranchii species landed at the study site had a decreasing population status. Keywords: Bintan, Elasmobranchii, Diversity, Conservation

Variabilitas Spasio-Temporal Suhu Permukaan Laut di Sebagian Pesisir Kabupaten Banyuasin Propinsi Sumatera Selatan Ningsih, Ellis Nurjuliasti; Agussalim, Andi; Barrus, Beta Susanto; Hartoni, Hartoni
JURNAL ENGGANO Vol. 9 No. 1 (2024)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jenggano.9.1.1-10

ABSTRACT The coastal waters of Banyuasin are shallow marine waters directly connected to the Bangka Strait. The waters are drained by several large rivers and many small rivers, and the movement of water masses is influenced by tidal activity. Sea surface temperature as one of the physical factors of water is important to study considering its role can be used as an indicator of water quality, an indicator of the habitat of aquatic organisms, as well as a basis for studying climate change and several physical processes in the ocean, such as upwelling and fronts. This research aims to map the spatio-temporal distribution of sea surface temperature. The methods used are the remote sensing method and field surveys using image data in 2022–2023. The results showed that there are differences in the distribution of sea surface temperature both spatially and temporally. In the western season, also known as the western season, the temperature range is found in the range of 27.77–29.88 oC. In the first transitional season, the temperature range is 27.39–31.56 oC; the temperature distribution in the eastern season ranges from 30.13–33.89 oC; and the temperature range in the second transitional season is 24.47–30.37 oC. The highest sea surface temperatures occur in the eastern season, and these highest temperatures are lower in the western season. The spatial distribution of sea surface temperature is generally lower in coastal waters in the west and transition II seasons and higher in the transition I and east seasons. Keywords: sea surface temperature, remote sensing, Banyuasin coastal waters, Variability

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INDONESIA