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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
Arjuna Subject : -
Articles 202 Documents
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Uji Kualitas DNA Darah Pada Kertas Whatman Yang Diisolasi Dengan CHELEX-100 Serta Variasi Waktu Penyimpanan Rita Maliza; Lutfi Sukma Pratiwi; Dyah Aryani Perwitasari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7936

Abstract

DNA extraction from dried blood spots was used for molecular analysis. Dried blood spots sample usually used FTA cards, but short-term use would come at a cost. This study aims to identify DNA extraction quality from dried blood spots in Whatman filter paper as an alternative storage sample. This study used 15 samples with three different storage time for 1, 3, and 7 days on 4°C. Dried blood samples were extracted using the Chelex-100 method, and qualitative were identified by electrophoresis. The DNA extraction was used as a template on PCR amplification of the gapdh gene. The result showed that DNA extraction showed band from 1, 3, and 7 days, and PCR amplification showed bands in 200 bp. In conclusions, DNA from dried blood samples was stored for 1, 3 and 7 days in Whatman filter paper were successfully extracted by the Chelex-100 method and can be used as a DNA template for PCR amplification.
Pemantauan Pasien Dengan Diagnosa Gagal Ginjal Kronik Di RSUD Sumber Rejo Bojonegoro Rinza Rahmawati Samsudin; Rahma Widyastuti; Nur Vita Purwaningsih
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.11130

Abstract

The kidneys are important organs that function to maintain blood composition by preventing the accumulation of waste and controlling the balance of fluids in the body. Chronic Kidney Disease (CKD) has become a major health problem of Indonesian people. Creatinine examination become one of the choices to identify the index of the kidney severity. The purpose of this study was to determine the description of creatinine levels of patients diagnosed with Chronic Kidney Disease (CKD). This research used descriptive method. The population of this study was patients with the diagnosis of Chronic Kidney Disease (CKD) who conduct the examination of creatinine levels in the installation of Pathology ClinicLaboratory which amounted to 100 patients during April to May. This research used the secondary data obtained and collected from the LIS (Laboratory Information System) and the medical record. The result of this study showed tht 73% of 100 patients diagnosed with Chronic Kidney Disease (CKD) are not normal, and the 27% data of creatinine levels is normal. Presentations of male patient who experienced levels of creatinine was high, namely 37 patients compared female. The incidence of patients with Chronic Kidney Disease (CKD) the highest range of productive age of 20-64 years was 50 patients (68%). The results of the research of the average content on patients diagnosed with Chronic Kidney Disease (CKD) obtained the creatinine level average of 4.01 mg/dl.   Keywords: Chronic Kidney Disease (CKD), Creatinine
Formulasi Dan Karakteristik Fisik Sediaan Plester Hidrogel Ekstrak Daun Ciplukan (Physalis angulata L.) Dan Batang Kayu Manis (Cinnamomum burmannii) Ria Hanistya; Karima Samlan
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.11151

Abstract

Diabetes mellitus is a degenerative disease caused by the damage of beta pancreatic cells. This caused many complication such as diabetic ulcer which left untreated can cause an open wound hard to heal thereby reducing the quality of life. Treatment of diabetic ulcer despite using antidiabetic also using an antibiotic therapy like Mupirocin. In its development, the use of natural ingredients is known to be used as adjuvant therapy for diabetic ulcer such as Ciplukan leaves and Kayu Manis bark. Both of the plant contain phytochemical compound like sinamaldehid, tannin, polyphenol and flavonoid which can work as wound healer. To increase its efficacy then Ciplukan leaves and Kayu Manis bark were formulated as hydrogel patch. Hydrogel patch are known has advantages like better penetration, hygienic and easy to administered Ciplukan (Physalis angulata L.) leaves and Kayu Manis (Cinnamomum burmannii) Bark extracted with maceration technique. The extracts then made into matrix type hydrogel patch with 10% HPMC and 5% PVA as backing patch. The backing patch and extract then placed on a square cast and dried under room temperature for 24 hours. The hydrogel patch then evaluated for physical characteristic including organoleptic aspect, weight uniformity, moisture content and pH. The results showed that Ciplukan leaves and Kayu Manis Bark hydrogel patch have a semi solid consistency, transparent greenish color and distinctive smell. The average pH is 5,5 and have average weight around 1,18 grams. The moisture contens of hydrogel patch is 2,42%. The Ciplukan leaves and Kayu Manis bark is able to formulated into hydrogel patch. The hydrogel patch is showing good appearance and having good properties as topical dosage form. The pH of the hydrogel patch is suitable with pH of the skin and have no intention to irritate the skin. Hydrogel patch also giving a cooling sensations in wound area thus increase patients convenience and acceptability.  Keywords: Diabetes mellitus, extract, drug delivery system, hydrogel patch, adjuvant therapy
Perbedaan Kadar Trigliserida Pada Sampel Darah Segera Disentrifugasi Dan Sampel Darah Dibekukan Selama 20 Menit Sebelum Disentrifugasi Reni Junika Familianti; Indah Sari; Bastian Bastian
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9580

Abstract

Trigliserida atau yang sering disebut triasilgliserol adalah salah satu jenis lemak yang terdapat dalam darah dan berbagai organ tubuh yang dibentuk dari gliserol dan lemak yang ada dalam makanan yang dikonsumsi secara berlebihan. Nilai trigliserida yang tinggi memiliki kaitan dengan timbulnya penyakit serangan jantung, stroke dan diabetes. Penelitian ini bertujuan untuk mengetahui perbedaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan selama 20 menit yang dilakukan di Balai Besar Laboratorium Kesehatan Palembang. Jenis penelitian yang digunakan adalah Cross sectional komparatif, dengan rancangan penelitian intact group comparison. Sampel berupa 30 serum segera disentrifugasi dan 30 serum dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Penelitian ini dilakukan mulai dari persiapan sampel, pengambilan bahan pemeriksaan, pengolahan bahan pemeriksaan, analisis dan hasil penelitian. Kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel serum segera disentrifugasi yaitu 0.826 mmol/L sedangkan kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel dibekukan 20 menit sebelum disentrifugasi yaitu 0.918 mmol/L. hasil uji dependent t test diketahui bahwa nilai signifikan adalah p = 0,342. Nilai p yang didapatkan adalah p > 0,05. Hasil penelitian dapat disimpulkan bahwa tidak ada perbedaan hasil pemeriksaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Kata kunci : Kadar Trigliserida, Segera Sentrifugasi, Dibekukan 20 MenitDaftar Pustaka: 31 (2013-2020)
Studi Pemeriksaan Sedimen Urine berdasarkan Karakteristik Pada Penikmat Kopi di Asrama Kiwal Brawijaya Surabaya Pipit Festy Wiliyanarti; Jilan Atrasina; Ellies Tunjung Sari Maulidiyanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.11135

Abstract

Coffee is a psychostimulant drink which can provide good benefits for the body if consumed in moderation. However, one of the effects in excessive coffee consumption over a long period of time that is not accompanied by adequate fluid intake is in fact able to affect sedimentation in urine. The caffeine content which has a lot of particles can cause the kidneys to be unable to reabsorb it. Therefore, it is excreted along with the urine and it causes the urine to be more concentrated. This causes increased sedimentation in the urine. The purpose determined the description of urine sediment examination on coffee connoisseurs in Asrama Kiwal in Brawijaya of Surabaya. The research type used descriptive research with a research sample of 40 people taken by purposive sampling. The results showed that the description of urine sediment examination on coffee connoisseurs at Asrama Kiwal in Brawijaya of Surabaya that 12 people (30%) had normal urine sediment and 28 people (70%) had abnormal urine sediment. Based on the characteristics of the respondents, the presence of abnormal urine sediment was found in the age category 30 years as many as 26 people (76%) with a frequency of coffee consumption > 3 times per day as many as 14 people (35%) in a series of coffee consumption for > 10 years as many as 27 people ( 67.5%) and the frequency of consumption of mineral water 2 litters per day as many as 24 people (60%). The conclusion of this study was that most of the coffee connoisseurs in Asrama Kiwal Brawijaya of Surabaya that experienced abnormal urine sedimentation.Keywords: Coffee, Urine Sediment
Uji Efektifitas Perasan Jahe (Zingiber officinale) Terhadap Pertumbuhan Jamur Candida albicans Fitrotin Azizah; Dita Artanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.11282

Abstract

Candidiasis is one of the most common infectious diseases caused by Candida sp. In 2017 the Mycology Division of Dr. Soetomo said there were 67 patients diagnosed with candidiasis. The use of natural ingredients as an alternative to traditional medicine is increasing because it is safe for consumption and does not cause side effects. One of the natural ingredients is Ginger (Zingiber officinale). The choice of using ginger juice (Zingiber officinale) is because the ingredients are easy to obtain and easy to apply by the community. This study aims to determine the antifungal effectiveness of ginger (Zingiber officinale) juice against the growth of Candida albicans. This research is experimental by doing the treatment which was repeated 4 times, namely the concentration of ginger juice 20%, 40%, 60%, 80%, 100%, negative control (aquadest) and positive control (ketoconazole). The inhibition zone examination method used the Kirby Bauer diffusion method which was characterized by the formation of a clear zone around the paper disc on MHA (Muller Hinton Agar) media. The inhibition zone formed was measured using a caliper, then the data obtained were analyzed using the Mann-Whitney test. From laboratory examination, it was found that the average diameter of inhibition formed with concentrations of 20%, 40%, 60%, 80%, and 100% was 0 mm, and the negative control (aquadest) was 0 mm. The results of statistical analysis showed that 0.05 obtained a significant value between the administration of juice concentrations of 20%, 40%, 60%, 80%, 100% and not given (negative control-aquadest) = 1,000. From these results, it was concluded that there was no significant difference between the concentrated juice of 20%, 40%, 60%, 80%, 100% and not given (negative control - distilled water)Keywords : Ginger (Zingiber officinale), Candida albicans
Uji Aktivitas Anti Bakteri Dari Formula Ekstrak Daun Ciplukan (Physalis angulata L.) dan Batang Kayu Manis (Cinnamomum burmannii) Secara In Vitro Test Fuad Muzakky; Malika Ilma Alkautsar; Anni Humayroh Syawalia; Nabila Mirza Azizi; Subhan Rullyansyah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9691

Abstract

Ciplukan leaves (Physalis angulata L.) and Cinnamon (Cinnamomum burmannii) are one of medicinal plants that contain antibacterial active compounds. This study aims to determine the antibacterial activity of the combination formula between extracts of ciplukan leaves and cinnamon sticks against the growth of Staphylococcus aureus bacteria in Vitro. Ciplukan leaves (Physalis angulata L.) and cinnamon (Cinnamomum burmannii) were extracted by the maceration method with 70% ethanol. Ciplukan leaf extract (Physalis angulata L.) and cinnamon (Cinnamomum burmannii) were tested against Staphylococcus aureus bacteria using the good method by measuring transmittance on a spectrophotometer at a wavelength of 580 nm with 3 repetitions, 100 ppml standard Septromycin solution as control positive and aquadest as a negative dick. Inhibition zone diameter of ciplukan leaf extract (Physalis angulata L.) x̄ = 12.46 mm, Cinnamon Extract (Cinnamomum burmannii) x̄ = 18.93 mm, while in the formulation group x̄ = 22.45 mm. One-way analysis of variance (ANOVA) of these data compared with Streptomycin activity (positive control) showed significant inhibitory activity by the formulated extract against S. aureus. Keywords: Antibacterial, Staphylococcus aureus,  Ciplukan leaves (Physalis angulata L.) and Cinnamon (Cinnamomum burmannii)
Analisis Kadar Timbal (Pb) Dan Serum Glutamic Pyruvic Transaminase (SGPT) Pada Pengemudi Ojek Online Di Kota Denpasar, Bali rahayu artini yuan; I Wayan Tanjung Aryasa; Putu Lakustini Cahyaningrum
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7815

Abstract

motorcycle taxi is a transportation service with communication technology in the world. The average mobility of online motorcycle taxi drivers is between 8-12 hours per day, this will certainly affect the physical condition and function of the drivers' organs, especially motorbike drivers. The purpose of this study was to determine the health status of online motorcycle taxi drivers, which was reviewed from liver function tests, namely serum glutamic pyruvic transaminase (SGPT) and exposure to heavy metal lead (Pb). This study used an experimental experimental research design pretest-posttest control group design. In this study, there was an increase in the levels of SPGPT and lead (Pb) for online motorcycle taxi drivers in Denpasar City with the SGPT level for male drivers being 14.14% and for women 19.62% and lead (Pb) levels for male drivers. -Male is 2.61% and female 5.14%.
Efektivitas Lama Perendaman Serbuk Kulit Jeruk Manis (Citrus sinensis) Terhadap Bilangan Peroksida Pada Minyak Jelantah Nastiti Kartikorini; Baterun Kunsah; Diah Ariana
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.11523

Abstract

Used cooking oil is the remaining cooking oil from the frying process. The use of cooking oil repeatedly at high temperatures and for a long time causes the peroxide number to increase. High peroxide levels can present a health hazard. Phenolic and flavonoid content contained in sweet orange peel is an antioxidant that acts as an antidote to free radicals so that it can slow down the oxidation of used cooking oil. The purpose of this study was to determine the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. This type of research is experimental with the number of sample repetitions for each treatment, namely 25 samples. The formulation of the problem of this research is whether there is an effect of the effectiveness of the long soaking time of the sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil. From the results of the examination, the average levels of peroxide numbers in used cooking oil that had been soaked were 1 day 22.7949 mEq, 2 days 19.5965 mEq, 3 days 13.5975 mEq and 4 days 9.5984 mEq. Statistical analysis using ANOVA showed the effect of the effect of soaking duration of sweet orange peel powder (Citrus sinensis) on the peroxide number in used cooking oil p (sig) = 0,000 which is less than 0.05.  Keywords:  Sweet Orange, Peroxide Number, Used Cooking Oil. 
Pembuatan Membran Antiseptik Dari Kombinasi Kitosan Dan Kulit Jeruk Nipis (Citrus aurantiifolia Agnes Ayu Biomi; rahayu artini yuan
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.10870

Abstract

As technology develops, air quality tends to decline. One of the air pollutants that is often encountered is bacteria that infect through the air such as Staphylococcus aureus. As a basic protection tool, a cloth mask with a membrane layer can be an alternative solution. One of the materials that can be used is chitosan and lime peel pectin. Lime peel produces essential oils that are used as stimulant, aromatherapy, and antimicrobial enhancers. This study aims to determine the results of characteristic tests, content analysis through phytochemicals and Staphylococcus aureus. The research method used is descriptive exploratory and experimental. The membrane mask made from chitosan of shrimp skin and lime peel has a characteristic thickness of 00.24 mm with a density of 0.60 - 0.90 g/cm3 with a textured heterogeneous surface. Has a water vapor transmission power of 0.00088 g/day/m2. It takes 27.54 days to completely decompose. Grapefruit peel essential oil contains secondary metabolites in the form of alkaloids, flavonoids, tannins, phenolics and triterpenoids. The membrane mask had Staphylococcus aureus antibacterial activity ranging from 14.05 mm. Keywords: Lime peel (Citrus aurantiifolia), Shrimp Skin Chitosan, Membrane mask, Antibacterial.

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