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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
Arjuna Subject : -
Articles 202 Documents
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THE EFFECT OF TOMAN (Chana Micropetes) EXTRACT ON THE IMPROVEMENT OF ALBUMIN LEVELS IN MENCIT/MICE (Mus musculus) Pipit Festi W
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v3i2.6047

Abstract

Hypoalbuminemia is a condition in which albumin levels are less than 3.8 g / dl. Management of hypoalbumin can be done on a  high high in protein such as toman fish extract (Chana Micropetes). Toman fish extract contains globular protein which can be used as an alternative diet in increasing albumin levels. The purpose of this study was to determine the effect of toman fish extract on increasing albumin levels in mencit (Mus musculus).               This research is an experimental research design with Pretest-Posttest Control Group Design. Samples using Mus musculus with the criteria of male sex, body weight 25-40 grm and 2 months old. The number of samples consisted of 32 animals consisting of 2 groups, 16 control groups and 16 treatment groups. Intervention by giving toman fish extract for 14 days. Research instruments, albumin levels and observation sheets. Data were tested statistically with Independent Sample t Test.               The results, this study showed the average value of albumin levels was 5.60 gr / dl in the treatment group so that an increase in albumin levels. There was a significant effect (p = 0,000 <α = 0.05) between the administration of toman fish extract to the increase in albumin levels in mencit (Mus Muculus).               Conclusion, giving toman fish extract can increase albumin levels in mencit. So that toman fish extract can be used as a high-protein alternative diet for hypoalbumin sufferers.Keywords: hypoalbumin, toman fish, (Chana Micropetes).
ANALISA CEMARAN LOGAM BERAT (Pb, Cd, Zn) PADA MAKANAN DAN MINUMAN KEMASAN KALENG DENGAN MENGGUNAKAN METODE Spektrofotometri Serapan Atom (SSA) Baterun Kunsah; Nastiti Kartikorini; Diah Ariana
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7604

Abstract

AbstractMakanan dan minuman yang dikemas dalam kaleng memiliki kemungkinan untuk terkontaminasi logam berat yang berasal dari komponen penyusun kaleng. Kontaminasi logam berat bisa menyebabkan keracunan dan berbahaya bila masuk ke dalam system metabolism tubuh dengan jumlah yang melebihi batas yang telah ditetapkan dalam SNI dan BPOM. Penelitian ini bertujuan untuk menganalisa Timbal (Pb), Kadmium (Cd) dan  seng (Zn) pada sarden, kornet, susu kaleng, sari buah, minuman berkarbonasi. pengambilan sampel dilakukan di dua lokasi berbeda yaitu pasar modern dan pasar tradisional, dengan tujuan agar dapat membandingkan perbedaan pada pasar modern dan pada pasar tradisional.Serapan logam diukur dengan menggunakan spektofotometri serapan atom (SSA) pada gelombang yang spesifik. Hasil penelitian adalah kadar cadmium (Cd) tidak teridentifikasi, kadar logam Timbal (Pb) pada sampel makanan dan minuman kaleng adalah 0.01 mg/kg, 0.018 mg/kg, 0.026 mg/kg, 0.012 mg/kg, 0.027 mg/kg, 0.027 mg/kg, 0.018 mg/kg, 0.015 mg/kg, 0.024 mg/kg dan 0.019 mg/kg, sementara itu cemaran Zn  adalah 0.188 mg/kg, 0.169 mg/kg, 0.193 mg/kg, 0.184 mg/kg, 0.129 mg/kg, 0.105 mg/kg, 0.107 mg/kg, 0.098 mg/kg, 0.176 mg/kg, 0.152 mg/kg. Semua sampel makanan dan minuman kaleng tidak ada yang melebihi batas maksimum cemaran yang telah di tetap kan dalam SNI dan Badan Pengawas Obat dan Makanan. Kadar cemaran logam berat Pb dan Zn pada sampel makanan dan minuman kaleng dari pasar tradisional lebih tinggi daripada sampel makanan dan minuman kaleng dari pasar modern              Kata kunci : Makanan-Minuman Kemasan Kaleng, SSA, Timbal, kadmium, seng
Evaluasi Analitik Hematology Analyzer Diatron Abacus 3 Pada Parameter Hematologi Rutin Di Laboratorium Hematologi Poltekkes Kemenkes Kalimantan Timur Suryanata Kesuma; Mahdiah Syumarliyanty; Agus Rudi Hartono
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.6467

Abstract

Entering the era of globalization, manual tools in clinical laboratories have been replaced by full automatic devices. One of them is the Diatron Abacus Hematology analyzer 3. A relatively new hematology analyzer is required for analytical evaluation. Analytical evaluation is an evaluation of Diatron Abacus 3 on Sysmex KX 21 as a standard in RSUD I.A Moeis Samarinda, and it is very important to do this to assess the performance of the tool. Analytical evaluation is done by determining the value of accuracy, precision, and total error and linearity of measurement results from routine hematological examination parameters, which are erythrocytes, leukocytes, platelets, hemoglobin, and hematocrit. To determine the results of the hematology analyzer evaluation of Abacus 3 Diatron Analyzers on Routine Hematology Parameters in the Hematology Laboratory of the Health Ministry of Health, East Kalimantan. This type of research is observational descriptive, using a total sampling technique, and a sample of 40 complete K3EDTA blood specimens. Data processing using Microsoft Excel and SPSS 20 applications, analyzed using descriptive statistics. The observations were still included in the criteria for acceptance, accuracy or inaccuracy (d%) in erythrocytes 1.8%, leukocytes 8.0%, platelets -5.3%, hemoglobin 2.3% and hematocrit -1.7%; Precision or impression (CV%) in erythrocytes 4.2%, leukocytes 11.1%, platelets 6%, hemoglobin 3.9% and hematocrit 4.5%; Total errors in erythrocytes were 8.7%, leukocytes 17.9%, platelets 23.6%, hemoglobin 8.8% and hematocrit 9.1; Linearity of the measurement results against the routine hematological cell count values performed using Abacus 3 and Sysmex KX 21 has a positive relationship. Acceptance values are still included in the LOA on all parameters examined and still meet the criteria; the accuracy/bias value is smaller than the true value of the parameter being examined, except for leukocytes, which is greater; the precision on the five parameters is greater than the CV% Abacus 3 insert kit; The total error obtained by the TE value is greater than the TEA in the parameters examined, except for smaller platelets; Linearity of the measurement results against the calculated hematology cell routine values performed using Abacus 3 and Sysmex KX 21 have a positive relationship, meaning an increase in measurement values using Abacus 3 is followed by an increase in measurement values using Sysmex KX 21.
ANALISI KADAR ALP (ALKALINE PHOSPATASE) DAN KHOLINESTERASE AKIBAT LAMA BEKERJA PADA PETUGAS FOGGING DI KOTA DENPASAR Ni Kadek Mona Fujiastuti Hendriani; Ni Putu Rahayu Artini; I Wayan Tanjung Aryasa
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v3i2.5843

Abstract

 Fogging is an effective way to prevent dengue fever in Indonesia. The side effects of fogging can have an impact on its officers by being exposed and inhaled by continuing to treat fumes that contain pesticides. So, that the liver function examination is carried out to determine the increase in ALP  (Alkaline Phospatase ) levels and decrease in cholinesterase levels due to a long time working on fogging officers in Denpasar. This aims to determine whether there is an increase in ALP levels and a decrease in the level of cholinesterase in fogging officers' blood samples. This type of research carried out statistical tests by displaying the lowest, highest value and average test results and deviations standards. From the examination results obtained, it was found that ALP levels in March 2020 with an average of 63.1 ± 0.23 IU / L, while in April 2020 with an average of 98.0 ± 0.23 IU / L, at cholinesterase levels in March 2020 with an average of 5.2 ± 0.01 kU / L, while in April 2020 the average was 6.5 ± 0.01 kU / L. It can be concluded that there was an increase in ALP with an average percentage increase of 0.88% and a decrease in cholinesterase levels in April higher than March, with an average percentage decrease of 0.29%.  
KORELASI ANTARA PROFIL HEMATOLOGI DENGAN PROCALCITONIN PADA PASIEN TERKONFIRMASI COVID-19 puspitasari Puspitasari; Andika Aliviameita; Evi Rinata; Dira Nadila
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7290

Abstract

Covid-19 is an infectious disease caused by SARS-CoV 2 and has spread very quickly. Several laboratory examinations were carried out on Covid-19 patients including molecular examinations, hematological profiles, and serology. This study aims to determine the relationship between leucocyte, d-dimer, hemoglobin and thrombocyte against procalcitonin in patients with Covid-19. This was an observational analytic study with cross sectional design. A sample of 50 was taken selectively at Aisyiyah Siti Fatimah Tulangan Hospital in November 2020 - January 2021. Multiple linear regression test results showed relationship between leucocyte with procalcitonin (b = -0.64; p = 0.426), d-dimer with procalcitonin (b = 2.702; p = 0.010), hemoglobin with procalcitonin (b = 0.656; p = 0.255), and thrombocyte with procalcitonin (b = 0.037; p = 0.002) . Based on the results, it is found that there is relationship between leucocyte, d-dimer, hemoglobin, and thrombocyte againts procalcitonin in Covid-19 patients.
Perbedaan Hasil Pemeriksaan Kromium Heksavalen (Cr VI) Dengan Pengompleks 1,5 Difenilkarbazida Pada Waktu 5 Menit Dan 15 Menit Secara Spektrofotometri UV-VIS Indah Sari; Pra Dian Mariadi; Rosnita Sebayang
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9567

Abstract

Differences of hexavalent chromium (Cr VI) examination results with complexing of 1,5 diphenylcarbazide at 5 minutes and 15 minutes by spectrophotometry uv-vis at (Balai Riset dan Standard Industri Palembang) Research and Industry Standard Center Palembang, 2017. Study Program of DIV Health Analyst, Faculty of Health Sciences of Musi Charitas Catholic University Palembang. Chromium (VI) is a carcinogenic component for humans, the amount of chromium that is safe to consume by humans is 50 - 200 μg a day. This research was an analytical comparative, conducted in The Research and Industry Standard Center Palembang. Samples were from pro-analytical chemical content made from chromium-acting susceptibility of 0,1 ppm and 0,2 ppm and the samples were given a complex of 1,5 diphenylcarbazide in the period of 5 minutes and 15 minutes. The obtained chromium examination results with complexing 1,5 diphenylcarbrazide in the period of 5 minutes were 0,0869 and 0,1775 and in the period of 15 minutes were 0,0932 and 0,1626. The data analyzed by using Wilcoxon p value and it was obtained p> 0,05. Based on this research, it was concluded that chromium examination with complexing of 1,5 diphenylcarbrazide in the period of 5 minutes and 15 minutes had no significant difference.
Potensi Metabolit Sekunder Isolat Aktinomycetes Sebagai Penghasil Senyawa Antibakteri Terhadap Methicillin Resistant Staphylococcus aureus (MRSA) Dari Tanah Mangrove Wonorejo Surabaya Anita Dwi Anggraini; Ayu Puspitasari; Christ Kartika Rahayuningsih
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7672

Abstract

Infectious diseases are estimated to be the cause of half of all deaths worldwide. About 50-75% of deaths in hospital are reported to be due to infection. This number continues to increase rapidly in line with the development of bacterial resistance to certain antibiotics. One of the causes of resistance due to antibiotic abuse. The number of resistance events that occur encourages new discoveries in order to overcome the problem of resistance. Actinomycetes are Gram-positive microscopic bacteria that are widespread in nature. These bacteria can live in various environmental conditions that contain lots of nutrients. Actinomycetes population increases in the presence of organic matter that undergoes decomposition. Acinomycetes have the ability to produce antimicrobial compounds. The need for antibiotic compounds and chemotherapy compounds that are effective with low toxicity to the host, and the resulting waste can be degraded by the environment, is a problem that needs serious attention. The existence of resistance to antibiotics causes the need to develop new types of antibiotics from natural ingredients and microorganisms that can control microbapatogens. Compounds obtained from natural materials, especially plants and microbes, provide promising results in the development of new antibiotic compounds, among the existing types of microorganisms, Actinomycetes are the most potential source of producing antibiotics. Apart from antibiotics, actinomycetes also produce bioactive compounds of high economic value in the health sector as antiviral and anti-cancer, while in agriculture as herbicides, insecticides, and anti-parasitic compounds. This causes the need to explore actinomycetes which have the potential to produce antibiotic compounds against bacteria. This study aims to determine the antibacterial activity of secondary metabolites of actinomycetes isolates against cuts infected with Staphylococcus aureus resistant methicillin (MRSA) in winstar rats (rattus norvegicus). This type of research is carried out in an experimental laboratory. The results showed that the potential for secondary metabolites of actinomysetes isolates against MRSA bacteria was classified as strong with an inhibitory index of 10.1.
GAMBARAN PEMERIKSAAN KADAR GLUKOSA DAN KOLESTEROL PADA LANSIA Nur Vita Purwaningsih; Fitrotin Azizah; Ellies Tunjung Sari Maulidiyanti; Dita Artanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v2i2.10872

Abstract

The increasing life expectancy based on WHO indicates an increase in the number of elderly people (elderly) so that there is an increase in health problems in the elderly due to the aging process which causes many changes in the body of the elderly. One of the changes in the body of the elderly is the blood glucose and cholesterol regulation system, resulting in an increase of more than normal. The purpose of this study was to determine the profile of glucose and cholesterol levels in the elderly. This research is observational. The population and sample in this study were the elderly in Karang Penang. The examination method used in this examination is the POCT (Point Of Care Test) examination method. The results showed that the elderly who had levels as much as 10 respondents (20%) samples had normal glucose levels, while as many as 40 respondents (80%) samples had high glucose. For cholesterol levels, 20 respondents (40%) samples had normal cholesterol levels, while 13 respondents (26%) samples had threshold cholesterol levels, then 7 respondents (14%) samples had high cholesterol levels. Keywords        : blood glucose, cholesterol and the elderly
Pemanfaatan Serum Hemolisis Dengan Penambahan Reagen Anti-Rh Terhadap Kadar Enzim Serum Glutamic Pyruvic Transminase (SGPT) Bastian Bastian; Indah Sari; Helnia Sari; Juwy Trianes
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9745

Abstract

Serum glutamate pyruvate transferase (SGPT) also known as alanine aminotransferase (ALT) is an enzyme found in liver cells and is effective in diagnosing hepatocellular destruction. Examination of SGPT activity using blood serum is often difficult due to insufficient blood volume or lysed serum conditions due to improper collection. This study aims to determine whether there is use of serum hemolysis using anti-rh reagents in the examination of SGPT enzyme levels. This type of research uses an experimental design - static group comparison. The sample will be taken by students of DIV Medical Laboratory Technology IKest Muhammadiyah Palembang. The research was carried out starting from patient preparation, taking examination materials, processing examination materials, analysis and examination results. The results of the ANOVA test showed that the significant value was p = 0.000. The p value obtained was p> 0.005. The results of the study found that there was a significant difference in the amount of SGPT in normal serum and serum with the addition of anti-Rh to hemolysis serum
Prevalensi Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA) Pada Peralatan Laboratorium kurniawan Kurniawan -; Eva Adaning Tyas; Supriyadi -
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7554

Abstract

Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%.  Keywords: laboratory equipment, MRSA, prevalence, S. aureus

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