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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
Arjuna Subject : -
Articles 202 Documents
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Penggunaan Berbagai Jenis Antikoagulan Terhadap Mutu Hasil Pemeriksaan Activated Partial Thromboplastine Time (APTT) Ellies Tunjung Sari Maulidiyanti; Fitrotin Azizah; Tri Ade Saputro; Firdausi Nuzula
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.14604

Abstract

Activated Partial Thromboplastine Time (APTT) is a type of hemostasis examination with citrate anticoagulant 3.2% ratio 1:9. Ethylene Diamine Tetraacetic Acid (EDTA) is one of the examination materials for hematology which is often used as an anticoagulant. Apart from EDTA, other types of anticoagulants are sodium citrate and potassium oxalate. The three anticoagulants work to bind calcium ions. The purpose of this examination is to determine the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APCT) examination. The method used in this study is an experimental study with 3 sample groups, each consisting of 9 samples in one group. The SPSS results showed a probability value of 0.000 with the Anova test, the average results of the APTT examination were administration of sodium citrate anticoagulant 25.9 seconds, potassium oxalate 70.92 seconds, and EDTA 37.5 seconds. Based on the results of these studies, there are significant differences related to the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APTT) examination.Keywords : Activated Partial Thromboplastine Time, Antikoagulans, 
Efektifitas Ekstrak Lidah Mertua (Sansevieria trifasciata var laurentii) Sebagai Antifungi Candida albicans Prasasti Kusumaning Paramastri; Muhammad Taufiq Qurrohman
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.13478

Abstract

Candidiasis is a disease caused by Candida albicans, this disease has a mortality rate of > 25%, mainly affecting adults, with a frequency of women 2 - 3 times more than men (Raningsih et al., 2018). A commonly used fungi drug is ketoconazole. but it has many side effects so it is necessary to find for alternatives to natural ingredients, namely the leaf of lidah mertua (Sansevieria trifasciata var laurentii) because it contains compounds saponins, flavonoids, triterpenoids, and steroids as antifungals The leaf of lidah mertua were extracted by maceration technique. The purpose of this research was to determine the effect of antifungi of ethanol extract the leaf of Lidah Mertua (Sansiviera trifasciata var Laurentii) against the fungus Candida albicans. This research was experimental using the disc diffusion method with a spread plate technique in the inhibition zone formed at concentrations of 25%, 20%, 15%, 10%, 5%, positive control and negative control. The results of the One way Anova test is known that differ significantly. From the results of this research, it can be concluded that the ethanol extract of the leaves of the leaf of lidah mertua (Sansevieria trifasciata var laurentii) has an effect on the antifungal activity of Candida albicans Keywords       : Candida albicans, Leaf of Lidah Mertua, Antifungal, Ketokonazole
Manajemen Pengambilan dan Pengelolaan Spesimen Darah di Laboratorium RSUD Wangaya Denpasar Anak Agung Ayu Eka Cahyani; Putu Ayu Parwati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.15518

Abstract

Kesalahan tahap pra-analitik memberikan kontribusi paling besar pada kesalahan laboratorium. Kesalahan pada tahap pra-analitik yang sering terjadi adalah hemolisis (53,2%), volume spesimen kurang (7,5%), tulisan tangan yang tidak bisa dibaca (7,1%), kesalahan identifikasi pasien, ada bekuan, vacum container yang salah/antikoagulan, volume antikoagulan yang tidak sesuai, spesimen diambil dari jalur infus, dan kesalahan waktu dalam pengambilan spesimen. Penelitian dilakukan untuk menganalisis tahapan pengambilan dan pengelolaan spesimen darah di laboratorium RSUD Wangaya Denpasar. Teknik pengambilan sampel dilakukan secara total sampling sebanyak 18 orang petugas ATLM di laboratorium Patologi Klinik. Data primer dikumpulkan dari hasil wawancara dan kuisioner untuk menganalisis pengetahuan terkait pengambilan dan pengelolaan spesimen darah. Hasil dengan skor tertinggi dan dalam kategori sangat baik yaitu pada pengambilan spesimen (97,8%). Skor terendah dan dalam kategori baik adalah pemberian identitas pasien (82,5%). Petugas laboratorium di RSUD Wangaya telah melakukan tahapan pengambilan dan pengelolaan spesimen darah sesuai peraturan Menteri Kesehatan RI tahun 2013 dan masuk dalam kategori sangat baik yaitu 91,48%.
Deteksi Jamur Candida albicans Pada Urine Penderita Infeksi Saluran Kemih Menggunakan Metode RT-PCR Retno Sasongkowati; Anita Dwi Anggraini; Dellanis Arina Putri
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14409

Abstract

                                                        ABSTRAKCandida albicans merupakan anggota flora normal yang terdapat pada selaput mukosa,  saluran pencernaan, saluran pernafasan, uretra, vagina, kulit dan kuku tangan dan kaki. Candida albicans dapat tumbuh pada berbagai macam pH, tetapi umumnya pertumbuhan akan lebih baik pada pH antara 4,5-6,5. Candida albicans dapat tumbuh dalam perbenihan pada suhu 28ºC – 37ºC. Jenis penelitian yang digunakan merupakan penelitian deskriptif kuantitatif. Penelitian ini bertujuan untuk untuk mengetahui adanya jamur Candida albicans pada urine penderita infeksi saluran kemih menggunakan metode Real Time PCR. Populasi dalam penelitian penderita infeksi saluran kemih. Infeksi saluran kemih merupakan infeksi keadaan klinis akibat berkembangbiaknya mikroorganisme yang menyebabkan inflamasi pada saluran kemih. Penelitian dilaksanakan di Laboratorium Parasitologi dan Laboratorium Biologi Molekuler pada bulan April 2022 hingga Mei 2022. Pada penelitian ini dilakukan beberapa tahapan pemeriksaan seperti isolasi kultur pada media Sabaroud Dextrose Agar, pemeriksaan makroskopis dan mikroskopis, pembuatan suspensi sel, uji ekstraksi, uji kemurnian DNA, optimasi dan pemeriksaan Real Time PCR dengan menunjukkan hasil berupa CT. Hasil penelitian menunjukkan pada pemeriksaan Real Time PCR ini didapatkan sejumlah 2 sampel dengan persentase 22,2% pada kode sampel S7 dengan nilai CT 28,50 dan kode sampel S9 dengan nilai CT 23,19 dan hasil negatif sejumlah 7 sampel dengan persentase 77,8%. Adapun faktor penyebab yang dapat mengakibatkan keberadaan jamur Candida albicans pada urine penderita infeksi saluran kemih seperti personal hygiene, penderita diabetes mellitus, alat kontrasepsi dan penggunaan antiseptik. 
Analisis Sensitivitas Dan Spesifisitas COVID-19 Ag Rapid Test Terhadap PCR Test Wisnu Murti; Dian Rachma Wijayanti; Wulan Fitriani Safari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.12148

Abstract

SARS-Cov-2 is the newest coronavirus caused Covid-19 which can infect human and pandemic, COVID-19 parameter testing can be done with serological tests used to detect antibody or antigen and also molecular test using PCR method. This research is aimed to compare the sensitivity and specificity between COVID-19 Ag rapid test and PCR test. This research is an analytical descriptive study with 421 samples were obtained from documentation study with probability systematic random sampling and by using secondary data taken from Laboratory Information System (LIS) at Siloam Hospitals Lippo Village Tangerang and the type of observational analytical research with cross sectional design. The analysis results show the sensitivity rate was 96.64%, specificity was 93.75%, PPV was 89.44%, NPV was 98.08%, LR (+) was 15.46, and LR (-) was 0.04. The conclusion of the research result, COVID-19 Ag rapid test is valid to be used as a diagnostic test because it has high rate of sensitivity and specificity according to WHO regulations.   
Potensi Ikan Tongkol Dan Ikan Lele Sebagai Media Alternatif Pertumbuhan Escherichia coli Muhammad Sholakhuddin Al-Ayubi; Pestariati Pestariati; Anita Dwi Anggraini; Diah Titik Mutiarawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14407

Abstract

Media pertumbuhan yang mengandung nutrisi yang dibutuhkan oleh bakteri dan yang sering digunakan untuk pertumbuhan bakteri yaitu media Nutrient Agar. Mahalnya harga media pertumbuhan bakteri mendorong para peneliti untuk menggunakan media pertumbuhan bakteri yang terbuat dari bahan-bahan alami yang mudah didapatkan dan tidak mengeluarkan biaya yang mahal. Ikan tongkol memiliki kandungan protein sebesar 26,46% dan ikan lele yang mempunyai kandungan protein sebesar 24,63%. Escherichia coli merupakan bakteri Gram negatif bersel satu, bersifat patogen dan dapat menyebabkan penyakit diare. Jenis penelitian yang dilakukan adalah Eksperimen. Hasil penelitian ini menunjukkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Tongkol yaitu pada variasi massa 5 gram yaitu 118 x 10-13 CFU/mL, sedangkan jumlah rata-rata koloni terbanyak pada pertumbuhan Escherichia coli dengan menggunakan media alternatif Ikan Lele yaitu pada variasi massa 5 gram yaitu 104 x 10-13 CFU/mL. Media substitusi Nutrient Agar dari tepung daging Ikan Tongkol dan Ikan Lele dengan variasi massa 1 gram, 3 gram dan 5 gram dapat menumbuhkan koloni bakteri Escherichia coli dengan jumlah dan karakteristik yang berbeda. Hal tersebut disebabkan oleh kandungan protein di dalam tepung daging Ikan Tongkol dan Ikan Lele yang dapat memenuhi kebutuhan nutrisi pertumbuhan Escherichia coli. Ikan tongkol dan ikan lele mempunyai potensi sebagai bahan dasar pembuatan media alternatif untuk pertumbuhan bakteri Escherichia coli ditandai dengan adanya pertumbuhan koloni bakteri pada media alternatif ikan tongkol dan ikan lele. Kata Kunci : Ikan Tongkol, Ikan Lele, Nutrient Agar, Escherichia coli
Antibakteri Cuka Apel Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus Secara In Vitro I Wayan Tanjung Aryasa; Ni Putu Rahayu Artini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14103

Abstract

Vinegar has the chemical name acetic acid derived from the Latin word acetum, acetic acid, ethanoic acid, or vinegar is an organic acid chemical compound that is the most important carboxylic acid in commerce, industry, and laboratories known as a sour taster. and aroma in food. But keep in mind that vinegar can work as a disinfectant. One type of vinegar that we know is apple cider vinegar. Based on the background that has been stated above, the problem formulated in this study is how apple cider vinegar inhibits the growth of Escherichia coli and Staphylococcus aureus bacteria. The analysis of this research is in the form of a quasi-experimental, namely by comparing the inhibitory power of apple cider vinegar and control on the growth of Escherichia coli and Staphylococcus aureus bacteria and post-test only control group design. Based on the results of the research that has been carried out, the results show that the diameter of the inhibition zone formed on Escherichia coli bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar is 12.33 ± 0.12 mm, 2.21 ± 0.12 mm and 7.42 ± 0.09 mm. While the diameter of the inhibition zone formed on Staphylococcus aureus bacteria for foreign apple cider vinegar, Indonesian apple cider vinegar, and local apple cider vinegar was 14.56 ± 0.46 mm, 0.00 ± 0.00 mm, and 10.36 ± 0.27 mm. So it can be concluded that apple cider vinegar has antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria.
Perbandingan Kadar C-Reactive Protein Terhadap Fase Pemberian Obat Anti Tuberkulosis Amalia Ovi Mustika Seno; Dita Pratiwi Kusuma Wardani; Supriyadi Supriyadi; Arif Mulyanto
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.10623

Abstract

Pulmonary tuberculosis is a disease caused by Mycobacterium tuberculosis. C-Reactive Protein (CRP) is produced in the body by the liver in response to inflammation caused by Mycobacterium tuberculosis infection. CRP as the main mediator of the non-specific immune system, and the concentration will increase to 1000 times higher than normal value in the event of tissue injury or infection. The aim of this study was to determine the comparison of CRP levels to the phase of anti-tuberculosis drug administration in pulmonary tuberculosis patients. The study was conducted by cross sectional study with a semi-quantitative method in June - July 2020 in Purwokerto Community Lung Health Center. The number of samples as much as 24 TB patients which is 12 TB patients in 0 month and 12 TB patients in 6 months. Data were analyzed by Mann Whitney Test. The mean rank in 0 month was 18.08 with median (min-max) 12 (12-24) mg / dL, whereas mean rank for 6 months was 6.92 with median (mix-max) was 0 (0- 12) mg / dL. Serum CRP levels in pulmonary TB patients in the 0-month treatment phase are higher than CRP levels in the 6-month treatment phase (p = 0,000).
Potensi Daun Kelor (Moringa oleifera) Terhadap Kadar Kadmium Dan Kreatinin Dalam Darah Sebagai Indikator Kerusakan Fungsi Ginjal Pada Tikus Putih Yang Terpapar Asap Rokok Christ Kartika Rahayuningsih; Riya Agustin; Tacik Idayanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14261

Abstract

High exposure of cigarettes in Indonesia causes various disturbances in the body's metabolism, especially in the kidney, which is due to the cadmium metal content. Cadmium induces oxidative stress by the formation of free radicals and decrease natural antioxidant activity in the kidney organs (nephrotoxic). One way to overcome nephrotoxic by decreasing blood creatinine, we can utilize Moringa oleifera (MO) which is known as a "miracle plant" and have high contents of flavonoid groups, phenols, gallic acid, ascorbic acid and carotenoids as antioxidants and natural metal chelating agents. This research aimed to analyze the potential of Moringa leaves in decreasing cadmium and creatinine levels in the blood of white rats due to exposure of cigarette smoke (inhalation) at doses 200, 400 and 800 mg/kgwb. The type of research was an experimental study with quantitative analysis conducted in October 2021 – July 2022. The independent variable was the doses of Moringa leaves extract and the dependent variable were the blood cadmium and creatinine levels of white rats. The blood cadmium level was measured with AAS and the creatinine level was measured with Jaffe method without deproteination by photometry. The results of blood cadmium and creatinine levels in the treatment of MO extract at dose 200 mg/kgwb were 1.288875 μg/L and 0.85 mg/dL; MO extract at dose of 400 mg/kgwb were 0.811 μg/L and 0.775 mg/dL; MO extract at dose 800 mg /kgwb were 0.86275 μg/L and 0.7 mg/dL. Based on statistical analysis using Kruskal wallis, the results of the p value on cadmium levels in the blood were 0.004 < 0.05 and the p value on creatinine levels was 0.003 < 0.05, showed that there were significant differences in blood cadmium and creatinine levels in the treatment groups. Moringa leaf extract (Moringa oleifera) at a dose of 400 mg/kgwb had good potential in overcoming kidney damage in terms of creatinine levels due to cadmium exposure through cigarette smoke, which was equivalent to 44.3756 grams of fresh Moringa leaves that can be implemented for human as an additional antioxidant for overcoming free radicals.                                                       Keywords : Moringa oleifera, Cigarettes Smoke, Cadmium, Creatinine, Rattus norvegicus
Perbedaan Kadar C-Reaktive Protein (CRP) Pada Sampel Serum dan Plasma K3EDTA Dengan Metode Imunoturbidimetri Helniasari Helniasari; Nurhidayanti Nurhidayanti; Bastian Bastian
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.13350

Abstract

Examination of levels of C-Reactive Protein (CRP) in the blood is one of the tests. that can detect inflammation at an early stage. C-Reactive Protein (CRP) is an inflammatory marker that is synthesized in the liver. CRP levels increase due to trauma, bacterial infection and inflammation (inflammation and tissue damage). CRP is synthesized by the liver and It released into the bloodstream for 6-10 hours after the acute inflammatory process and acute destruction. Tissue detection CRP is also one of several proteins often referred to as the acute phase and It is used to monitor changes in the acute inflammatory phase associated with many infectious and autoimmune diseases. Examination of C-Reactive Protein (CRP) used serum and plasma samples of K3EDTA. C-Reactive Protein levels were measured using the Immunoturbidimetric method. This type of research was cross sectional. The study was conducted at the Clinical Pathology Laboratory, Faculty of Science and Technology, Institute of Health Sciences and Technology Muhammadiyah Palembang on December 21, 2021 with samples were 30. The results of the study had an average value in serum samples of 6.3 mg/L and an average value in plasma. K3EDTA 6.5 mg/L. Wilcoxon test results obtained p value = 0.000. The conclusion of the study was that there were differences in the examination of C-Reactive Protein (CRP) levels in serum and plasma samples of K3EDTA using the immunoturbidimetric method.

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