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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
Arjuna Subject : -
Articles 202 Documents
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HUBUNGAN LAMA PENGGUNAAN KB IUD DENGAN KADAR HB PADA AKSEPTOR KB IUD Nur Hidayatul Ainiyah; Syuhrotut Taufiqoh; Enny Juniati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v3i2.16721

Abstract

The IUD is the primary choice in the recommended long-term use of contraception. IUDs are widely used by women of childbearing age 20-35 years and have bleeding complications. This bleeding can cause a decrease in haemoglobin levels, so it is at risk of causing anemia. This research is analytical research with a cross-sectional design. This study aimed to determine the relationship between the duration of the IUD use and haemoglobin levels in family planning acceptors in the Tanah Kedinding Surabaya Health Center work area in 2020 with a sample of 11 IUD acceptors. Based on the results of the study, it was found that from the 11 respondents studied, 8 respondents (72.72%) were women of childbearing age users of long-term use IUDs, there were 5 people (62.50%) were anemic and as many as 3 respondents (27.27%) who were not anemic. Meanwhile, of the 3 (27.27%) women with IUDs using short-term IUDs, one respondent (33.33%) was anemic, and two respondents (66.7%) were not anemic. The Chi-Square test analysis results show that the calculated X2 value > X2 table is 7.236 > 3.841. This study concludes that there is a relationship between the duration of IUD use and Hb levels in IUD acceptors in the Tanah Kalikedinding Health Center work area in 2020. It is expected that health workers will check Hb levels at the start of IUD insertion and during acceptors' return visits and give Fe tablets to acceptors Kb long-term IUDKeywords        : duration, IUD use, haemoglobin level
KANDUNGAN KADAR PROTEIN PADA ASI BAYI 0-6 BULAN Fitrotin Azizah; Fulatul Anifah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 2 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v3i2.16725

Abstract

Breast milk has advantages compared to other milk, including the temperature of breast milk being the same as body temperature, cleanliness being guaranteed, and the content of breast milk plays an essential role in the growth of brain cells and the development of the nervous system. This study aims to describe the content of protein levels in breast milk. The samples used in this study were five samples of breast milk obtained from mothers who had children aged 0-6 months in the Surabaya area, using the Kjeldahl method. The average protein content of breast milk (is 12.764%). The protein content of breast milk has a higher protein content in laboratory tests because it adapts to the needs of the baby. Breast milk has a high protein content. The protein in breast milk is mostly whey protein rather than casein. The higher casein content will form relatively hard lumps in the baby's stomach, making it difficult to digest. The whey in formula milk contains lactoglobulin, which can cause allergies in babies. Mothers are expected to follow WHO recommendations to provide exclusive breastfeeding for 0-6 months and continue breastfeeding until the child is two years old.Keywords : Protein, content, breast milk, babies
Stabilitas Antioksidan Buah Kurma (Phoenix dactylifera L.) Pada Suhu Pemanasan Dengan Metobe ABTS Wieke Sriwulan
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5 No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.18048

Abstract

Free radicals are compounds that are very dangerous for the body because they can attack and cause cell damage in the body, therefore antioxidant compounds are needed that can counteract the presence of these free radicals. One of the fruits that contain antioxidant compounds is dates (Phoenix dactylifera L.) which contain flavonoid compounds that act as antioxidants. The purpose of this study was to determine the antioxidant activity at heating temperatures of 400C, 500C, and 650C. The extraction method used in this study is the maceration method with 80% ethanol as solvent. Testing of antioxidant activity using the method of reducing free radicals, namely the ABTS method and using vitamin C as a comparison control to show antioxidant activity. ABTS is a method of determining antioxidant activity obtained from the oxidation of potassium persulfate with diammonium salt. Absorbance measurements were carried out using a UV-Vis spectrophotometer with a wavelength of 520 nm. Data analysis was carried out using linear regression equations to determine the IC50 value in the sample of dates (Phoenix dactylifera L.). The IC50 value indicates the antioxidant activity in a sample, namely the smaller the IC50 value, the higher the antioxidant in a sample. The results of data analysis using the Kruskal Wallis test obtained a significance value of 0.104 which indicates that > 0.05 which means there is no significant difference between each treatment group. From the results of the analysis data, it is known that there is no effect of heating temperature on the antioxidant stability of dates, this is also strengthened by the value of antioxidant activity in the linear regression equation where the value of antioxidant activity in each treatment group has an equally strong antioxidant activity value. based on the Blois classification. Keywords : Phoenix dactylifera L, Antioxidant and Extract 
Korelasi Kadar High Sensitivity C-Reactive Protein (hs-CRP) Dan Kolesterol Low Density Lipoprotein (LDL) Pada Perokok Aktif Dengan Aktivitas Fisik Aliya Ayu Rizqiyah; Evy Diah Woelansari; Suhariyadi Suhariyadi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14363

Abstract

Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.
The Potential of Averrhoa bilimbi Juice As An Alternative Reagent in Proteinuria Examination Erna Kristinawati; Nutri Farda Nur; idyatul hasanah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.10604

Abstract

Background: Proteinuria indicates a kidney disorder and provides important prognostic information in diagnosing kidney disease. To determine the presence of protein in the urine, a urinalysis examination is carried out.  Proteinuria examination generally uses the heating method with acetic acid, sulfosalicylic acid, and concentrated nitric acid as reagents. In Indonesia, the use of acetic acid for proteinuria examination is still the primary choice. An alternative reagent is needed that can replace the function of acetic acid. The objective of this study is to determine the potential of Averrhoa Blimbi juice as an alternative reagent for proteinuria examination.Methods: This was a Pre-experimental study with a completely randomized design. This study used urine samples from a patient with chronic kidney disease. Urine samples were examined using 6% acetic acid reagent as a control and using Averrhoa bilimbi juice with various concentrations. Each juice concentration was examined twice so that the experimental units in this study were 40 units. The Kruskal-Wallis test was used to determine the potential of Averrhoa bilimbi  as an alternative reagent.Results: At concentrations of 5%-20% showed the same positive results as 6% acetic acid, namely +2, concentration 25%-35% resulted in +3, and other concentration resulted in +4. The results of data analysis showed p=0.04 (p<0.05). This shows that Averrhoa bilimbi has the potential as an alternative reagent for proteinuria examination.Conclusion: Averrhoa bilimbi  juice with a 5%-20% concentration has the potential as an alternative reagent for 6% acetic acid to check proteinuria levels.Keywords: Averrhoa bilimbi ; alternative reagents; proteinuria; acetic acid; urinalysis
Uji Daya Hambat Ekstrak Segar Buah Pare (Momordica charantia Linn.) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae Dan Streptococcus pneumoniae chylen setiyo rini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14494

Abstract

Saluran pernapasan merupakan organ tubuh manusia yang banyak mengandung populasi bakteri (sekitar 13 spesies) yang dapat menjadi patogen seperti Klebsiella pneumoniae dan Streptococcus pneumoniae. Oleh karena itu, perlu adanya upaya mengendalikan Klebsiella pneumoniae dan Streptococcus pneumoniae dengan bahan dari bahan alam, salah satunya yaitu pare (Momordica charantia Linn). Buah pare (Momordica charantia Linn) telah terbukti khasiatnya sebagai obat yang memiliki kandungan senyawa seperti alkaloid, saponin, fenolik, streoid, flavonoid, dan tanin yang memiliki kemampuan sebagai antibakteri. Jenis penelitian ini adalah penelitian eksperimental. Dengan metode difusi kertas cakram atau Kirby-Bauer dengan 5 konsentrasi yaitu : 20%, 40%, 60%, 80%, 100%, dan digunakan antibiotik kloramfenikol sebagai (kontrol+). Pengujian dilakukan sebanyak 3 kali pengulangan. Data yang peroleh kemudian diuji menggunakan uji Anova one way dengan nilai signifikansi 0,05. Hasil penelitian menunjukkan bahwa ekstrak segar buah pare efektif terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae. Konsentrasi optimum pada bakteri Klebsiella pneumoniae diperoleh pada konsentrasi 100% dengan diameter sebesar 21,31 mm dan konsentrasi 80% dengan diameter sebesar 22,36 mm pada bakteri Streptococcus pneumoniae, sedangkan hasil uji ANOVA one way yang telah dilakukan terhadap diameter zona hambat bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae diperoleh nilai signifikan p=0,000 dari hasil tersebut diketahui bahwa nilai <0,05 yang menunjukkan ada pengaruh yang nyata dari pemberian ekstrak segar buah pare terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae.
Volunteer Program Skrining Dan Percepatan Vaksinasi Covid-19 Pada Ibu Hamil Di Surabaya Umi Ma'rifah; Baterun Kunsah; Nova Elok Mardiyah; Fulatul Anifah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.17996

Abstract

The increasing number of Covid-19 cases, especially the presence of a new Delta variant which causes a risk of severe symptoms in pregnant women, the Indonesian government has established a screening program and implementation of the Covid-19 vaccination as stated in circular letter HK.02.01/I/2007/2021. Based on preliminary studies carried out on pregnant women in the city of Surabaya, there are 90% percent of pregnant women who are still hesitant and afraid to vaccinate. One form of effort being made is the development of an application that facilitates screening and accelerates vaccination of pregnant women, namely the VMil application. The purpose of this study was to find out how the Volunteer program through the V-MiL application affects pregnant women in carrying out Covid-19. This type of research is pre-experimental with one group pre-test and post test design. The dependent variable is the respondent's participation in the implementation of the Covid-19 vaccine, while the independent variable is intervention by volunteers using the V-MiL application. The research sample for pregnant women who had not been vaccinated was 40 people using a purposive sampling technique. Data collection with questionnaires and interviews. The results of the study the majority of respondents aged 21-30 years (58%), gestational age 13-27 weeks (78%). After the intervention the respondents had good knowledge 18 (45%), lack of knowledge 22 (55%), negative attitude positive attitude 16 (40%), negative attitude 24 (60%), strong motivation 15 (38%), weak motivation 25 (63%) to vaccinate. Respondents did the Covid-19 vaccine as many as 15 (38%) and 25 (63%) respondents did not do the vaccine. Analysis using the Paired sample T test obtained a value of λ of 0.001 <0.005 which indicated that there was a significant influence on the intervention given by volunteers to the respondents. Keywords                    : Volunteers, Covid-19 Vaccines, Pregnant Women
Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur Mulya Fitrah Juniawan; Dita Artanti; Yuni Gayatri; AINUTAJRIANI AINUTAJRIANI
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15898

Abstract

Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge. Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.
Identifikasi Mutasi Gen kelch 13 Penanda Resistensi Pada Plasmodium falciparum Dengan Pengobatan ACT Setelah 3 Hari Di Manokwari Papua Barat Sarlince Agustin Nesan; Budi - Santosa; Mudyawati Kamarudin
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15840

Abstract

Malaria is almost found all over the world, including Indonesia. WHO data on malaria case transmission in 2020, namely 241 million and 627,000 deaths. Female Anopheles mosquitoes are the main factor in the transmission of parasites, one of which is Plasmodium falciparum which is most dangerous to cause complications to death. Data from the Ministry of Health 2019 the highest transmission in Papua Province is 216,380 cases. The malaria elimination program uses ACT as a treatment therapy for malaria. The first appearance of ACT resistance in Cambodia was in 2008 to Plasmodium falciparum which was associated with the presence of a Kelch 13 gene mutation. The purpose of this study was to identify a mutation of the Kelch 13 gene markers of resistance in Plasmodium falciparum with ACT treatment after 3 days. This type of exploratory study used a population of all patients at the Amban Manokwari Health Center in West Papua, with a total sampling in August-September 2022. Of the 51 and 11 H3 samples met the inclusion criteria using RDT and Microscopic methods. The DNA isolated sample using Favorgen kit then amplified PCR and electrophoresis gel agarose. Then were 7 amplicons seen in the DNA band (±200bp) and confirmed results from PT. Science Genetics (±100bp), then 1 amplicon is continued for sequencing. The sequence results were analyzed using the Bioedit and Mega IX softwere with the alignment of the sequence results. Changes in nucleeid bases and amino acids were obtained so that the mutation of the Kelch 13 gene occurred, 3 mutation variants: substitution (transition & transversion), silent mutation (C3T codon 1 cysteine-cysteine), and missense mutation (T4A codon 2 tryptophan-serine). It can be concluded that the Plasmodium falciparum treatment ACT carriers the Kelch 13 gene mutation as one of the markers of resistance. Keywords: ACT, Plasmodium falciparum, Kelch 13 Gene Mutation.
Pengaruh Air Rebusan Daun Sirsak (Annona muricata Linn) Terhadap Kadar Asam Urat Pada Wanita Usia Lebih Dari 45 Tahun Di Desa Besuki Kecamatan Lumbir Kabupaten Banyumas Retno Sulistiyowati; Agny Kusumaningrum
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.11932

Abstract

Asam urat merupakan senyawa kimia hasil akhir dari metabolisme purin dalam tubuh. Wanita mengalami hiperurisemia meningkat pada wanita setelah menopause, karena mengalami penurunan hormon estrogen. Daun sirsak mengandung flavonoid yang bersifat sebagai antioksidan yang dapat mengurangi terbentuknya asam urat. Tujuan penelitian menganalisis pengaruh pemberian air rebusan daun sirsak terhadap kadar asam urat pada wanita usia di atas 45 tahun di Desa Besuki. Penelitian berjenis kuantitatif dengan menggunakan metode pre-experimental design tipe one group pretest – posttest. 14 responden mengkonsumsi air rebusan daun sirsak sebanyak satu gelas sehari selama 2 minggu. Hasil penelitian menunjukkan penurunan kadar asam urat setelah pemberian air rebusan daun sirsak, dengan rerata kadar asam urat sebelum diberi perlakuan 6,0714±1,06946 mg/dL sedangkan rerata kadar asam urat setelah diberi perlakuan 5,4071±1,36971 mg/dL. Rerata nilai perbedaan kadar asam urat sebelum dan sesudah diberi perlakuan sebesar 0,6429±0,72707 mg/dL.

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