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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
Arjuna Subject : -
Articles 202 Documents
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Korelasi Kadar High Sensitivity C-Reactive Protein (hs-CRP) Dan Kolesterol Low Density Lipoprotein (LDL) Pada Perokok Aktif Dengan Aktivitas Fisik Rizqiyah, Aliya Ayu; Woelansari, Evy Diah; Suhariyadi, Suhariyadi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14363

Abstract

Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.
Pemanfaatan Teri Jengki (Stolephorus indicus) Dan Layang Deles (Decapterus macrosoma) Sebagai Media Alternatif Pertumbuhan Escherichia coli Nurhasanah, Armelia Gitasari; Pestariati, Pestariati; Anggraini, Anita Dwi; Astuti, Sri Sulami Endah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14452

Abstract

ABSTRAK Nutrient agar merupakan jenis media umum yang sering digunakan pada laboratorium, harga media ini relatif mahal. Nutrient agar berbentuk padat, terdiri atas campuran ekstrak daging, pepton serta agar. Ikan teri jengki dan ikan layang deles jumlahnya melimpah di Indonesia. Ikan teri jengki (Stolephorus indicus) mengandung protein 51.54%, sementara ikan layang deles (Decapterus Macrosoma) mengandung protein sebesar 57,89%. Escherichia coli digunakan sebab bakteri ini merupakan salah satu jenis mikroorganisme kontrol positif yang direkomendasikan berdasarkan Data Sheet Nutrient Agar OXOID. Penelitian ini bersifat eksperimental dengan tiga perlakuan pada masing-masing bahan yaitu variasi massa 3 gram, 4 gram dan 5 gram yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Hasil penelitian menunjukkan jumlah koloni rata-rata Escherichia coli yang tumbuh pada kontrol positif sebanyak 118 x 1013, sementara pada media ikan teri jengki variasi massa 3 gram adalah 72 x 1013 CFU/mL, 4 gram sebanyak 85 x 1013 CFU/mL, 5 gram sebanyak 94 x 1013 CFU/mL. Pada media ikan layang deles variasi 3 gram sebanyak 88 x 1013 CFU/mL, 4 gram sebanyak 96 x 1013 CFU/mL, 5 gram 108 x 1013 CFU/mL. Variasi massa yang paling baik dan mendekati hasil kontrol positif (nutriet agar) pada ikan teri jengki dan ikan layang deles adalah variasi massa 5 gram.Kata kunci: ikan teri jengki, ikan layang deles, Escherichia coli, nutrient agar
Uji Daya Hambat Ekstrak Segar Buah Pare (Momordica charantia Linn.) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae Dan Streptococcus pneumoniae rini, chylen setiyo
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14494

Abstract

Saluran pernapasan merupakan organ tubuh manusia yang banyak mengandung populasi bakteri (sekitar 13 spesies) yang dapat menjadi patogen seperti Klebsiella pneumoniae dan Streptococcus pneumoniae. Oleh karena itu, perlu adanya upaya mengendalikan Klebsiella pneumoniae dan Streptococcus pneumoniae dengan bahan dari bahan alam, salah satunya yaitu pare (Momordica charantia Linn). Buah pare (Momordica charantia Linn) telah terbukti khasiatnya sebagai obat yang memiliki kandungan senyawa seperti alkaloid, saponin, fenolik, streoid, flavonoid, dan tanin yang memiliki kemampuan sebagai antibakteri. Jenis penelitian ini adalah penelitian eksperimental. Dengan metode difusi kertas cakram atau Kirby-Bauer dengan 5 konsentrasi yaitu : 20%, 40%, 60%, 80%, 100%, dan digunakan antibiotik kloramfenikol sebagai (kontrol+). Pengujian dilakukan sebanyak 3 kali pengulangan. Data yang peroleh kemudian diuji menggunakan uji Anova one way dengan nilai signifikansi 0,05. Hasil penelitian menunjukkan bahwa ekstrak segar buah pare efektif terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae. Konsentrasi optimum pada bakteri Klebsiella pneumoniae diperoleh pada konsentrasi 100% dengan diameter sebesar 21,31 mm dan konsentrasi 80% dengan diameter sebesar 22,36 mm pada bakteri Streptococcus pneumoniae, sedangkan hasil uji ANOVA one way yang telah dilakukan terhadap diameter zona hambat bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae diperoleh nilai signifikan p=0,000 dari hasil tersebut diketahui bahwa nilai <0,05 yang menunjukkan ada pengaruh yang nyata dari pemberian ekstrak segar buah pare terhadap pertumbuhan bakteri Klebsiella pneumoniae dan Streptococcus pneumoniae.
UJI FITOKIMIA DAN ANTIBAKTERI FRAKSI AIR KULIT BATANG TANAMAN TURI (Sesbania grandiflora L.) TERHADAP BAKTERI Staphylococcus aureus Ni Luh Made Rahayu Widya Lestari; Desak Putu Risky Vidika Apriyanthi; Ni Putu Rahayu Artini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.14515

Abstract

Staphylococcus aureus is a bacteria that can cause skin and soft tissue infections such as urinary tract infections, pneumonia, mastitis, and meningitis. Staphylococcus aureus infections are usually treated with antibiotics, but most antibiotics are resistant to the bacteria and have side effects of toxicity to the body. One alternative to finding new antibacterials from natural ingredients is to use turi plants. This study aims to determine the content of secondary metabolites in the water fraction of the bark of turi (Sesbania grandiflora L.) and to determine the antibacterial activity of the water fraction of the bark of turi (Sesbania grandiflora L.) against Staphylococcus aureus. This research is an experimental study, using the bark fraction of the turi plant. The fraction was obtained after the fractionation process from the bark extract of turi (Sesbania grandiflora L.) so that the water fraction was divided into several concentrations, namely 25%, 50%, 75%, and 100%. Antibacterial activity test was carried out by well diffusion method. The results of this study indicate that the water fraction of the bark of turi (Sesbania grandiflora L.) contains secondary metabolites of alkaloids, tannins, saponins, steroids, and phenolics. The antibacterial activity produced was included in the moderate category at a concentration of 25% with an inhibition zone of 9.70 ± 0.14 mm, a strong category at concentration of 50% and 75% with an inhibition zone of 12.37 ± 0.25 mm, and 18.47 ± 0.23 mm, in addition to the category of very strong at a concentration of 100% with an inhibition zone of 23.47 ± 0.09 mm.
PERBEDAAN KADAR SERUM KREATININ PADA PEMINUM ARAK TINGKAT RINGAN, SEDANG DAN BERAT DI KELURAHAN RENON, KECAMATAN DENPASAR SELATAN, KOTA DENPASAR maheswari, dyah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.14549

Abstract

Konsumsi alkohol dapat menyebabkan perubahan struktur dan fungsi ginjal serta mengganggu kemampuan ginjal untuk mengatur volume, elektrolit dan komposisi cairan dalam tubuh. Pemeriksaan serum kreatinin merupakan salah satu parameter yang penting untuk mengetahui fungsi ginjal. Penelitian ini merupakan penelitian observasional dengan rancangan cross sectional analitik untuk mengetahui perbedaan kadar serum kreatinin pada peminum arak tingkat ringan, sedang dan berat di Kelurahan Renon, Kecamatan Denpasar Selatan, Kota Denpasar. Dari hasil penelitian didapatkan data peminum ringan berjumlah 7 sampel dengan kadar serum kreatinin paling rendah yaitu 0,6 mg/dL dan paling tinggi yaitu 1,4 mg/dL. Peminum sedang berjumlah 12 sampel dengan kadar serum kreatinin paling rendah 0,8 mg/dL dan paling tinggi 1,8 mg/dL. Peminum berat berjumlah 11 sampel dengan kadar serum kreatinin paling rendah 1,0 mg/dL dan paling tinggi yaitu 2,5 mg/dL. Berdasarkan hasil uji One-Way ANOVA untuk ketiga kelompok peminum bernilai signifikansi 0,000. Hasil uji LSD kelompok peminum rendah – berat bernilai p = 0,000 dan kelompok peminum sedang – berat bernilai p = 0,000.
Pengaruh Variasi Waktu Penundaan Pemisahan Serum Terhadap Hasil Pemeriksaan Glukosa Azizah, Febrianti Nur
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.14594

Abstract

Tahapan pra-analitik merupakan faktor yang penting dalam pemeriksaan di laboratorium. Tahapan ini meliputi persiapan untuk pasien, pemberian identitas pada spesimen, penampungan spesimen, perlakuan pada spesimen, pengiriman spesimen, penyiapan dan pengolahan spesimen. Serum yang terbentuk setelah proses sentrifugasi sebaiknya segera digunakan untuk pemeriksaan dan apabila harus dilakukan penundaan maka serum sebaiknya segera dipisahkan dari bekuan darah , dipindahkan pada wadah serum dan disimpan dalam lemari pendingin. Tidak semua laboratorium melakukan penyimpanan serum yang sesuai. Hal tersebut berpeluang terjadinya metabolisme spesimen yang disebabkan sel hidup sehingga berpengaruh pada stabilitas. Tujuan penelitian ini untuk mengetahui pengaruh variasi waktu penundaan pemisahan serum terhadap hasil pemeriksaan glukosa. Jenis penelitian ini adalah eksperimental dengan desain penelitian One Group Pretest-Pottest Design. Penelitian dilakukan pada bulan April 2022 di Laboratorium Kimia Klinik Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Digunakan 10 sampel serum dari mahasiswa dengan kadar glukosa normal. Sampel serum diberikan 5 perlakuan penundaan pemisahan yaitu 0, 1 ,2, 3, 4 jam. Kadar glukosa diperiksa dengan metode GOD-PAP pada alat fotometer 5010 vs+. Data yang terkumpul dianalisis dengan Uji Repeated Measure Anova . Rata-rataa hasil pemeriksaan glukosa yang dilakukan segera, penundaan 1 jam, 2 jam, 3 jam dan 4 jam adalah 78,1 mg/dL; 75,2 mg/dL, 71,8 mg/dL; 66,9 mg/dl dan 65,1 mg/dL. Hasil analisis uji statistik menunjukkan bahwa ada pengaruh variasi waktu penundaan pemisahan serum terhadap hasil pemeriksaan glukosa dengan nilai sig 0,000. Kata Kunci : Glukosa, serum, penundaan pemisahan
Modifikasi Metode Kalium Bikromat Untuk Uji Kualitatif Alkohol Dalam Urine Wulandari, Ayu Nurislami
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.15456

Abstract

Alcohol is a psychoactive substance that can cause dependence. Alcohol as an intoxicant can affect various structures and processes in the central nervous system and increase the risk of intentional and unintentional injury. One of the direct risks due to alcohol consumption is an accident while driving a vehicle and accidents at work. Rate alcohol It is measured in various body fluids including urine. This study aims to determine the sensitivity of the modified potassium dichromate method for qualitative test of alcohol in urine without filter paper and water bath. This research is a laboratory experimental study, the data obtained were analyzed in a descriptive way, namely seeing the color change of the reaction between the alcohol sample in urine and the Potassium bichromate reagent to green or blue with a modified working method. Tested all showed positive results with the modified Potassium bichromate method. A color change from green to blue is read as a positive alcohol result. The conclusion of this study is that the modified Potassium bichromate method is effective for the qualitative alcohol test process and there is an increase in the sensitivity of the test.
Identifikasi Mutasi Gen kelch 13 Penanda Resistensi Pada Plasmodium falciparum Dengan Pengobatan ACT Setelah 3 Hari Di Manokwari Papua Barat Nesan, Sarlince Agustin; Santosa, Budi -; Kamarudin, Mudyawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15840

Abstract

Malaria is almost found all over the world, including Indonesia. WHO data on malaria case transmission in 2020, namely 241 million and 627,000 deaths. Female Anopheles mosquitoes are the main factor in the transmission of parasites, one of which is Plasmodium falciparum which is most dangerous to cause complications to death. Data from the Ministry of Health 2019 the highest transmission in Papua Province is 216,380 cases. The malaria elimination program uses ACT as a treatment therapy for malaria. The first appearance of ACT resistance in Cambodia was in 2008 to Plasmodium falciparum which was associated with the presence of a Kelch 13 gene mutation. The purpose of this study was to identify a mutation of the Kelch 13 gene markers of resistance in Plasmodium falciparum with ACT treatment after 3 days. This type of exploratory study used a population of all patients at the Amban Manokwari Health Center in West Papua, with a total sampling in August-September 2022. Of the 51 and 11 H3 samples met the inclusion criteria using RDT and Microscopic methods. The DNA isolated sample using Favorgen kit then amplified PCR and electrophoresis gel agarose. Then were 7 amplicons seen in the DNA band (±200bp) and confirmed results from PT. Science Genetics (±100bp), then 1 amplicon is continued for sequencing. The sequence results were analyzed using the Bioedit and Mega IX softwere with the alignment of the sequence results. Changes in nucleeid bases and amino acids were obtained so that the mutation of the Kelch 13 gene occurred, 3 mutation variants: substitution (transition & transversion), silent mutation (C3T codon 1 cysteine-cysteine), and missense mutation (T4A codon 2 tryptophan-serine). It can be concluded that the Plasmodium falciparum treatment ACT carriers the Kelch 13 gene mutation as one of the markers of resistance. Keywords: ACT, Plasmodium falciparum, Kelch 13 Gene Mutation.
Pengaruh Waktu Fermentasi Pada Produksi Bioethanol Dari Molase yuan, rahayu artini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15887

Abstract

The corona virus outbreak first occurred at the end of 2019 in Wuhan, China. This virus then spread rapidly throughout the world, including Indonesia. So, countries in the world take steps to lock down. In Indonesia, large-scale social restrictions have been implemented in big cities with a high prevalence of the spread of the virus. Efforts to control the virus include limiting mobility, wearing masks, frequently washing hands or using hand sanitizers. Hand sanitizers are products made from ethanol which are increasingly limited and expensive during the Covid-19 pandemic. The purpose of this study is to make bioethanol with a variation of the fermentation time made from molasses, so as to produce ethanol content above 70%. This research is an experimental research. Production of bioethanol based on molasses, aquades, NPK, urea, and yeast culture of Saccharomycess cerevisae with time variations of 4, 5, and 6 days and distilled at a temperature of 500C. Testing the ethanol content using gas chromatography mass spectrometry. The results of this study, the fourth day of fermentation produces ethanol content of 50.23±0.42% with an average volume of 490 mL; day 5 produced ethanol content of 62.92±0.38% with an average volume of 385 mL; and the 6th day yielded ethanol content of 72.11±0.46% with an average volume of 243 mL. So, the 6th day of fermentation is the best fermentation to produce bioethanol as a raw material for hand sanitizer with ethanol content above 70%.
Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur Juniawan, Mulya Fitrah; Artanti, Dita; Gayatri, Yuni; AINUTAJRIANI, AINUTAJRIANI
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15898

Abstract

Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge. Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.

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