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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
Published by Universitas Muhammadiyah Surabaya
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions
Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
Arjuna Subject : -
Articles 202 Documents
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CAMPURAN INFUSA SINGKONG (Manihot esculenta), KACANG KEDELAI (Glycine max (L.) Merrill) DAN EKSTRAK RAGI SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI Staphylococcus aureus Martha Atik Martsiningsih; Fardhiasih Dwi Astuti; Menik Kasiyati; Suyana Suyana; Ullya Rahmawati; Muji Rahayu; Ajeng Puspa Aeni
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19863

Abstract

The composition of nutrients needed by bacteria varies greatly such as peptone, meat extract, and agar. Nutrient Agar is one of the media used as a bacterial culture that has a composition such as protein and carbohydrates in the form of meat extract and peptone that suits the needs of most bacteria. The carbohydrate content in cassava, protein in soybeans, and nitrogen sources in yeast extract can be combined into alternative media used as bacterial growth media to determine whether the alternative media combination of cassava carbohydrate source infuse, soybean infuse, and yeast extract can be used to grow Staphylococcus aureus bacteria. This research is a pure experimental study with Post Test-Only Control Design research design. The mean growth in the number of colonies of Staphylococcus aureus bacteria on alternative media was 78,25 x  CFU/ml and NA media was 94.19 x   CFU/ml with a mean difference of 15.94 x  CFU/ml or 16.92%. The mean growth diameter of the number of colonies of Staphylococcus aureus bacteria on alternative media was 2.63 x  CFU/ml and NA media was 2.66 x   CFU/ml with a mean difference of 0.03 x  CFU/ml or 1.12%. The percentage of effectiveness of Staphylococcus aureus bacterial growth is 83.07% or moderately effective and the percentage of effectiveness of Staphylococcus aureus bacterial proliferation is 98.87% or effective. Alternative media mixture of cassava infuse (Manihot esculenta crantz), soybean (Glycine max (L.) Merrill) and yeast extract can be used to grow Staphylococcus aureus bacteria.
Perbandingan Variasi Konsentrasi Sabun Cuci Piring Terhadap Kualitas Pewarnaan Hematoksilin Eosin Pada Tahap Deparafinasi Tri Widia Irianti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20070

Abstract

Salah satu tahapan dalam pewarnaan Hematoksilin Eosin yaitu deparafinasi.  Deparafinasi adalah proses pelunturan parafin dari jaringan. Xilena merupakan agen deparafinasi yang paling banyak digunakan, namun bersifat toksik dan berbahaya jika masuk ke dalam tubuh. Alternatif lain yang dapat digunakan sebagai pengganti xilena yaitu sabun cuci piring. Sabun cuci piring terbuat dari bahan utama yaitu surfaktan. Bahan aktif ini berfungsi menurunkan tegangan permukaan air sehingga dapat melepaskan parafin yang menempel pada permukaan sediaan. Penelitian ini betujuan untuk mengetahui adanya perbedaan kualitas pewarnaan Hematoksilin Eosin pada organ hepar tikus putih (Rattus norvegicus) yang dideparafinasi menggunakan xilena dan sabun cuci piring konsentrasi 0,5%, 1,0%, 1,5%, dan 1,7%. Jenis penelitian eksperimen sungguhan dengan rancangan Posttest only control group design. Hasil penelitian kualitas pewarnaan Hematoksilin Eosin yang dideparafinasi menggunakan xilena dan sabun cuci piring 0,5%, 1,0%, 1,5%, dan 1,7% didapatkan hasil pewarnaan yang baik dengan persentase berturut-turut 100%, 64%, 68%, 88%, dan 88%. Disimpulkan bahwa terdapat perbedaan bermakna kualitas pewarnaan Hematoksilin Eosin yang dideparafinasi menggunakan xilena dan sabun cuci piring konsentrasi 0,5%, 1,0%, 1,5%, dan 1,7%. Kelompok konsentrasi yang memiliki perbedaan bermakna antara sabun cuci piring dengan xilena yaitu konsentrasi 0,5% dan 1,0%, sedangkan pada konsentrasi 1,5% dan 1,7% tidak terdapat perbedaan bermakna atau memiliki kualitas yang sama berdasarkan uji statistik. Disarankan penggunaan sabun cuci piring konsentrasi 1,5% dan 1,7% sebagai alternatif pengganti xilena pada tahap deparafinasi.
EKSTRAK DAUN JATI MUDA UNTUK PEWARNA PEMERIKSAAN SEDIMEN URINE Doni Setiawan; Atun Farihatun; Ary Nurmalasari; Euis Tia Istianah; Mujahidah Basarang; Ellies TS Maulidiyanti2
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20647

Abstract

Urinalysis examination is needed to find information and determine the condition of the kidneys, urinary tract or other organs such as the liver, pancreas, and bile ducts. A urinary sediment examination aims to see organic and inorganic elements in the urine by preparing using Sternheimer Malbin stain reagents. Sternheimer Malbin stain has the disadvantage that it is a synthetic chemical. Stain from natural ingredients becomes a solution as an alternative to reduce the use of synthetic chemicals. Natural stains with the same characteristics and widely tested for bacterial and fungal stains are natural stains from teak leaf extract. Young leaf extract contains anthocyanins in red colour. This study aimed to determine the ability of ethanol extract from young leaves as a natural stain for urine sediment examination. The research method is an experiment with the Static Group Comparison research design. The population and samples in this study are teak leaves determined at the Galuh University Laboratory of the Biology Study Program with the Latin name (Tectona grandis L.f.). The results of the Fisher Exact test obtained a p (value) <0.050, so there was no significant difference between sediments without stain, Sternheimer Malbin, and ethanol extract of young leaves in colouring urine sediments. The conclusion is that the dye of ethanol extract from young teak leaves effectively colours the components of urine sediment, namely epithelial cells, leukocyte cells, and erythrocyte cells with red colour. Keywords      : Anthocyanins, Alternative Dyes, Sternheimer malbin
EFEKTIFITAS PEMBERIAN HIDROGEL EKSTRAK DAUN PEGAGAN (Centella asiatica) TERHADAP PERTUMBUHAN Staphylococcus aureus Amira Muhtadina R.A; Niken Rositasari; Aprilia Retno Sriwijayanti; Riska Putri Novianty; Farida Mumtazza Alkautsar; Vella Rohmayani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.22024

Abstract

Eksim atau dermatitis atopik, merupakan masalah kulit yang dapat mengganggu penampilan,dan  membuat mereka tidak percaya diri. Hal ini karena eksim dapat menyebabkan gatal pada kulit. Gatal yang digaruk berlebihan akan menyebabkan terjadinya inflamasi, sehingga menyebabkan kulit mudah terkena infeksi. Pengobatan eksim selama ini memanfaatkan terapi non farmakologis dan farmakologis. Hasil penggunaan terapi ini membutuhkan banyak waktu, dan biaya. Oleh sebab itu dibutuhkan pengobatan alternative berupa sediaan hidrogel dari ekstrak daun pegagan (Centella asiatica). Daun pegagan (Centella asiatica) adalah tanaman yang memiliki sifat antimikroba. Kandungan aktif daun pegagan memiliki kemampuan untuk menghentikan pertumbuhan bakteri. Tujuan penelitian ini adalah untuk menentukan konsentrasi ekstrak daun pegagan dan seberapa efektif sediaan hidrogel untuk menghentikan perkembangan Staphylococcus aureus. Ekstraksi daun pegagan menggunakan proses maserasi dengan pelarut etanol. Sementara untuk mengevaluasi sifat antibakteri dilakukan dengan metode sumuran. Ekstrak dibuat dalam konsentrasi 2,5%, 5%, 7,5%, 30%, 50%, 75%, serta perlakuan kontrol positif dengan menggunakan antibiotik Clindamycin. Berdasarkan hasil penelitian tersebut didapatkan  rata-rata diameter zona bening sebesar 15,25 mm. Pada konsentrasi 2,5% mendapatkan zona bening sebesar 4,5 mm, pada konsentrasi 5% mendapatkan zona bening sebesar 5 mm, dan pada konsentrasi 7,5% mendapatkan zona bening sebesar 7 mm. Sedangkan pada perlakuan ke-2 dengan konsentrasi 30%, 50%, 75% mendapatkan hasil 24 mm, 27 mm, dan 27 mm dengan control positif sebesar 24 mm. Berdasarkan hasil tersebut menunjukkan bahwa ekstrak daun pegagan memiliki aktivitas antibakteri terhadap Staphylococcus aureus dengan konsentrasi 75% bersifat sensitive mampu menghambat laju pertumbuhan bakteri Staphylococcus aureus dengan terbentuknya zona bening sebesar 27 mm. Keywords : Eczema, Hydrogel, Gotu Kola Leaf, Staphylococcus aureus
Hubungan Konsumsi Ikan Laut Terhadap Kadar Kolestrol Total Pada Nelayan Di Desa Tamberu Timur Waras Budiman; Ellies Tunjung S. Maulidiyanti; Rahma Widyastuti; Rinza Rahmawati Samsudin
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.23602

Abstract

Konsumsi ikan laut yang mempunyai kandungan yang kaya akan protein dan omega-3 serta berkualitas tinggi dan bagus bagi kesehatan, akan tetapi jika mengkonsumsi secara berlebihan dapat menyebabkan lemak jenuh serta dapat meningkatkan kadar kolesterol yang tinggi. Tiap harinya nelayan menghasilkan ikan, hasil tangkapan itu sebagian dijual dan sebagian dikonsumsi sehari-hari. Berdasarkan hasil wawancara dengan nelayan, mereka bekerja sebagai nelayan dalam waktu lebih dari 5 tahun. Kebiasaan nelayan tersebut yaitu tiap hari mengkonsumsi ikan hasil tangkapan, sebanyak lebih dari 3 kali dalam sehari. Tujuan penelitian ini adalah untuk mengetahui hubungan konsumsi ikan laut dengan kadar kolesterol total pada nelayan di desa tamberu timur. Metodeyang digunakan yaitu metode kuantitatif dengan jenis penelitian observasional analitik dengan desain cross sectional. Populasi yang dalam penelitian ini adalah nelayan di Desa Tamberu Timur. Teknik sampling yang digunakan pada penelitian ini adalah menggunakan probability sampling didapatkan 33 responden yang memenuhi syarat kriteria inklusi dan eksklusi. Hasil yang didapat kadar kolesterol yang tinggi yaitu terdapat 20 responden (60.7%), sedangkan kadar kolesterol yang normal sebanyak 13 orang (39.3%). Setelah dilakukan analisis data dengan SPSS didapatkan hasil kadar kolesterol sebesar 0,005< 0,05 sehingga dapat disimpulkan bahwa H1 diterima yang berarti terdapat hubungan antara mengkonsumsi ikan laut dengan kadar kolesterol pada nelayan di Desa Tamberu Timur. Kata kunci : Kolestrol total, Konsumsi Ikan laut
Identifikasi Bakteri di Ekosistem Mangrove Kecamatan Candi sebagai Agen Bioremediasi Pencemaran Logam Berat Cu, Pb, dan Hg di peraiaran Pesisir Wonorejo Nur Hidayatullah Romadhon
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.28175

Abstract

Bioremediation of heavy metals using bacteria has been widely developed due to the high accumulation of heavy metals (Cu, Pb, and Hg) in ecosystems, largely driven by industrial activities. The toxic effects of these metals on living organisms and the environment require immediate solutions through biological approaches. This study aimed to identify and analyze the potential of indigenous bacteria from mangrove sediments in Candi District as bioremediation agents for heavy metals Cu, Pb, and Hg in vitro. This research employed a descriptive-exploratory design and was conducted at the Microbiology Laboratory, Universitas Muhammadiyah Surabaya, from July to November 2023. Bacterial isolation was performed using the pour plate method and purified with the streak plate method on Nutrient Agar medium. Preliminary screening was carried out on Nutrient Agar supplemented with Cu, Pb, and Hg at concentrations of 100, 250, 350, and 500 ppm. The reduction ability of bacterial isolates was tested on Salmonella Shigella Agar (SSA) medium using the Langmuir method. Isolation of bacteria from mangrove sediments yielded nine isolates, all of which demonstrated the ability to degrade Cu, Pb, and Hg. Among them, isolate T1, identified as Pseudomonas aeruginosa (Gram-negative), exhibited the highest reduction capacity, achieving 82% for Cu, 74% for Pb, and 72.61% for Hg.
Perbedaan Pertumbuhan Bakteri Staphylococcus aureus Dengan Pemberian Perasan Daun Anting-Anting Dan Perasan Daun Bahagia Artanti, Dita; Radiawati, Eka
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v3i1.4012

Abstract

ABSTRACT               Gram Positive Bacteria, one of which is Staphylococcus aureus is a type of bacteria that is a major cause of nosocomial infection due to surgical procedures and treatment equipment in hospitals and poisoning in several regions in Indonesia. Staphylococcus aureus is commonly found in skin, nose, mouth, eye, finger, intestinal, and liver lesions. Juice of Anting-anting leaves (Acalyhpa indica L.) and happy leaf plants (Dieffenbachia bowmanii) are known to have medical benefits. Especially in its ability to produce metabolites that function as anti-bacterial compounds. This study aims to determine the differences in the growth of Staphylococcus aureus colonies which were given the leaves of Anting-anting and the leaves of Happy leaves. The research method uses the liquid dilution method in which the Staphylococcus aureus bacteria is incubated with the leaves of the Anting-anting and the leaves of the Happy leaves with a concentration of 100% at 37 ° C overnight. Then grown on Mannitol Salt Agar (MSA) media. The results showed that there was no difference in the growth of Staphylococcus aureus bacteria colonies that had been given the treatment of Juice of Anting-anting leaf (Acalyhpa indica L.) and happy leaf plants (Dieffenbachia bowmanii) which were marked by the absence of colony growth. So that the concentration of 100% in both leaves of juice is the best concentration that can be used to control the growth of Staphylococcus aureus.  Keywords : Anting-anting Leaves, Bahagia Leaves, Staphylococcus aureus
The Potential of Averrhoa bilimbi Juice As An Alternative Reagent in Proteinuria Examination Kristinawati, Erna; Nur, Nutri Farda; hasanah, idyatul
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.10604

Abstract

Background: Proteinuria indicates a kidney disorder and provides important prognostic information in diagnosing kidney disease. To determine the presence of protein in the urine, a urinalysis examination is carried out.  Proteinuria examination generally uses the heating method with acetic acid, sulfosalicylic acid, and concentrated nitric acid as reagents. In Indonesia, the use of acetic acid for proteinuria examination is still the primary choice. An alternative reagent is needed that can replace the function of acetic acid. The objective of this study is to determine the potential of Averrhoa Blimbi juice as an alternative reagent for proteinuria examination.Methods: This was a Pre-experimental study with a completely randomized design. This study used urine samples from a patient with chronic kidney disease. Urine samples were examined using 6% acetic acid reagent as a control and using Averrhoa bilimbi juice with various concentrations. Each juice concentration was examined twice so that the experimental units in this study were 40 units. The Kruskal-Wallis test was used to determine the potential of Averrhoa bilimbi  as an alternative reagent.Results: At concentrations of 5%-20% showed the same positive results as 6% acetic acid, namely +2, concentration 25%-35% resulted in +3, and other concentration resulted in +4. The results of data analysis showed p=0.04 (p<0.05). This shows that Averrhoa bilimbi has the potential as an alternative reagent for proteinuria examination.Conclusion: Averrhoa bilimbi  juice with a 5%-20% concentration has the potential as an alternative reagent for 6% acetic acid to check proteinuria levels.Keywords: Averrhoa bilimbi ; alternative reagents; proteinuria; acetic acid; urinalysis
Pengaruh Air Rebusan Daun Sirsak (Annona muricata Linn) Terhadap Kadar Asam Urat Pada Wanita Usia Lebih Dari 45 Tahun Di Desa Besuki Kecamatan Lumbir Kabupaten Banyumas Sulistiyowati, Retno; Kusumaningrum, Agny
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.11932

Abstract

Asam urat merupakan senyawa kimia hasil akhir dari metabolisme purin dalam tubuh. Wanita mengalami hiperurisemia meningkat pada wanita setelah menopause, karena mengalami penurunan hormon estrogen. Daun sirsak mengandung flavonoid yang bersifat sebagai antioksidan yang dapat mengurangi terbentuknya asam urat. Tujuan penelitian menganalisis pengaruh pemberian air rebusan daun sirsak terhadap kadar asam urat pada wanita usia di atas 45 tahun di Desa Besuki. Penelitian berjenis kuantitatif dengan menggunakan metode pre-experimental design tipe one group pretest – posttest. 14 responden mengkonsumsi air rebusan daun sirsak sebanyak satu gelas sehari selama 2 minggu. Hasil penelitian menunjukkan penurunan kadar asam urat setelah pemberian air rebusan daun sirsak, dengan rerata kadar asam urat sebelum diberi perlakuan 6,0714±1,06946 mg/dL sedangkan rerata kadar asam urat setelah diberi perlakuan 5,4071±1,36971 mg/dL. Rerata nilai perbedaan kadar asam urat sebelum dan sesudah diberi perlakuan sebesar 0,6429±0,72707 mg/dL.
Sensitivitas Pemeriksaan Covid-19: Insulated Isothermal PCR (iiPCR) Dan Reverse Transcription PCR (RT-PCR) Yunita, Lisa; Wijayanti, Dian Rachma; Riyanti, Apriani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.12441

Abstract

Covid-19 (Corona Virus disease 2019) is a new type of disease caused by a virus from the Corona Virus group, namely SARS-CoV-2. Covid-19 may cause respiratory system disorders, ranging from mild symptoms such as flu to lung infections, such as pneumonia. Laboratory diagnoses for Covid-19 disease generally include hematological (complete blood examinations), and molecular or a combination of serology and molecular. PCR examination that can be carried out using the Insulated Isothermal PCR and Reverse Transcription PCR methods. This research is descriptive observational research. Data were collected at Grha Kedoya Hospital, West Jakarta, North Kedoya. The result showed a sensitivity of iiPCR score 82,6%, which is relatively lower than gold-standard RT-PCR. Our research suggested that RT-PCR is still an effective and sensitive method for Covid-19 examination.

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