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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST
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Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik
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Articles 202 Documents
Hubungan Antara Personal Hygiene Dan Status Anemia Dengan Kejadian Infestasi Pediculus Humanus Capitis Pada Santri Kelas 1 Madrasah Tsanawiyah Di Pondok Pesantren “ Assaasunnajah “ Ateuk Lung Ie Kecamatan Ingin Jaya Provinsi Aceh Besar Dwi Haryatmi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19094

Abstract

Pediculosis humanus capitis is a blood-sucking parasite that spends its entire life cycle on the human scalp. Poor personal hygiene can affect pediculosis capitis infection. In school children, the chronic infestation of pediculosis capitis causes anemia and makes children lethargic and sleepy. The aim of the study was to determine the relationship between personal hygiene and anemia status with the incidence of pediculus humanus capitis in first grade students at madrasah tsanawiyah at the "Assaasunnajah" Islamic boarding school Ateuk lung ie, Wanna Jaya District, Aceh Besar Province. The research design used is descriptive analytic cross sectional approach. The sampling technique of this research is total sampling. The instruments in this study were observation and measurement. Based on the data analysis that has been done, with a total of 30 respondents, poor personal hygiene was obtained by 7 respondents with a percentage of 23.3%. For the incidence of pediculus humanus capitis, 7 respondents stated that a pediculus was found with a percentage of 23.3%. While Anemia Status based on Hb results, 2 respondents were declared Anemia with a percentage of 7%. The conclusion of this study based on the results of the chi square test showed that there was no relationship between personal hygiene and the incidence of pediculus humanus capitis because the test results obtained a p-value of 0.708 > confidence probability of 0.05 and there was a relationship between anemia and the incidence of pediculus humanus capitis in class 1 students of madrasah tsanawiyah at the "Assaasunnajah" Islamic boarding school Ateuk lung ie, Want Jaya District, Aceh Besar Province because the test results obtained a p-value of 0.008 < 0.05 confidence probability. Keywords   : Pediculosis, Pediculosis humanus capitis , Personal hygiene, Anemia
KAJIAN KADAR TIMBAL PADA RAMBUT PETUGAS OPERATOR STASIUN PENGISIAN BAHAN BAKAR UMUM DI KAWASAN INDUSTRI Fini Ainun Qolbi Wasdili; Annisa Ulfa Septiana Arisanti; Wikan Mahargyani; Ganthina Sugihartina
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19262

Abstract

Increasing population growth, industrialization and the use of motorized vehicles are the factors causing the increase in air pollution. One of the particles contained in the exhaust gas emissions produced is lead. This study was aimed at the operators of the Public Fuel Filling Station in the Industrial Area of West Bandung District who are vulnerable to lead exposure from various pollutant sources. The purpose of this study was to describe the highest and lowest levels of lead accumulated in the hair of gas station operators. The method used is descriptive method. The research sample was taken from respondents who have worked as gas station operators for >1 year. The sample used was 19 gas station operators. Data was collected by filling out a questionnaire followed by laboratory examination. The results showed that 5 people had lead levels exceeding the normal threshold and 14 others had normal lead levels with an average of 13.52 g/g. The highest lead content in hair was 110.75 g/g and the lowest level was -3.50 g/g. With a threshold value of lead levels in hair that is <12 mg/g. The head of the gas station is advised to apply regulations on the use of appropriate PPE for gas station operators to minimize lead exposure. For further research, it is recommended to monitor lead (Pb) levels with different specimens.   Keywords      : Lead, Hair, Gas Station, Industry, Motor Vehicle
Efektivitas Daun Kelor Terhadap Kadar Kadmium Dan LDL Kolesterol Dalam Darah Sebagai Indikator Penyempitan Pembuluh Darah (Aterosklerosis) Pada Tikus Putih Yang Terpapar Asap Rokok Rahayuningsih, Christ Kartika; Agustin, Riya
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.19837

Abstract

Tobacco consumption in Indonesia is a health epidemic that has direct or indirect toxicity due to exposure of cigarette smoke which contains harmful substances, especially cadmium metal. Cadmium’s exposure can increase the risk of coronary heart disease due to increased LDL cholesterol. One of the cadmium toxicity treatment by utilizing Moringa oleifera leaves as a natural antioxidant considering the flavonoids, saponins, ascorbic acid, carotenoids, phenolics, interquinones as cadmium chelating agents and β-sitosterol content. Therefore, it was necessary to determine the effectiveness of Moringa leaves extract in lowering blood cadmium and LDL cholesterol levels as indicators of atherosclerosis in white rats exposed to cigarettes smoke. The type of research was an experiment with quantitative analysis, conducted at the nutrition laboratory of the Faculty of Public Health Airlangga University and the clinical chemistry laboratory of Surabaya Health Polytechnic in March-July 2023. The independent variable was the doses of Moringa leaf extracts, while the dependent variables were blood cadmium and LDL cholesterol levels of white rats. The analysis of blood cadmium levels used an atomic absorption spectrophotometer and used precipitation methods by a photometer for LDL cholesterol levels. Based on statistical analysis using one way anova showed that blood cadmium levels had a significant value of < 0.05, followed by post hoc tests it was found that treatment group 2 had significant differences from normal groups. Moringa leaves extract dose of 1500 mg/kgbw has been effective in lowering cadmium and LDL cholesterol levels against the risk of atherosclerosis for 14 days. Keywords : Atomic Absorption Spectrophotometry, Cadmium, LDL Cholesterol, Moringa oleifera, photometer, Rattus norvegicus
CAMPURAN INFUSA SINGKONG (Manihot esculenta), KACANG KEDELAI (Glycine max (L.) Merrill) DAN EKSTRAK RAGI SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI Staphylococcus aureus Martha Atik Martsiningsih; Fardhiasih Dwi Astuti; Menik Kasiyati; Suyana Suyana; Ullya Rahmawati; Muji Rahayu; Ajeng Puspa Aeni
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19863

Abstract

The composition of nutrients needed by bacteria varies greatly such as peptone, meat extract, and agar. Nutrient Agar is one of the media used as a bacterial culture that has a composition such as protein and carbohydrates in the form of meat extract and peptone that suits the needs of most bacteria. The carbohydrate content in cassava, protein in soybeans, and nitrogen sources in yeast extract can be combined into alternative media used as bacterial growth media to determine whether the alternative media combination of cassava carbohydrate source infuse, soybean infuse, and yeast extract can be used to grow Staphylococcus aureus bacteria. This research is a pure experimental study with Post Test-Only Control Design research design. The mean growth in the number of colonies of Staphylococcus aureus bacteria on alternative media was 78,25 x  CFU/ml and NA media was 94.19 x   CFU/ml with a mean difference of 15.94 x  CFU/ml or 16.92%. The mean growth diameter of the number of colonies of Staphylococcus aureus bacteria on alternative media was 2.63 x  CFU/ml and NA media was 2.66 x   CFU/ml with a mean difference of 0.03 x  CFU/ml or 1.12%. The percentage of effectiveness of Staphylococcus aureus bacterial growth is 83.07% or moderately effective and the percentage of effectiveness of Staphylococcus aureus bacterial proliferation is 98.87% or effective. Alternative media mixture of cassava infuse (Manihot esculenta crantz), soybean (Glycine max (L.) Merrill) and yeast extract can be used to grow Staphylococcus aureus bacteria.
Perbandingan Variasi Konsentrasi Sabun Cuci Piring Terhadap Kualitas Pewarnaan Hematoksilin Eosin Pada Tahap Deparafinasi Tri Widia Irianti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20070

Abstract

Salah satu tahapan dalam pewarnaan Hematoksilin Eosin yaitu deparafinasi.  Deparafinasi adalah proses pelunturan parafin dari jaringan. Xilena merupakan agen deparafinasi yang paling banyak digunakan, namun bersifat toksik dan berbahaya jika masuk ke dalam tubuh. Alternatif lain yang dapat digunakan sebagai pengganti xilena yaitu sabun cuci piring. Sabun cuci piring terbuat dari bahan utama yaitu surfaktan. Bahan aktif ini berfungsi menurunkan tegangan permukaan air sehingga dapat melepaskan parafin yang menempel pada permukaan sediaan. Penelitian ini betujuan untuk mengetahui adanya perbedaan kualitas pewarnaan Hematoksilin Eosin pada organ hepar tikus putih (Rattus norvegicus) yang dideparafinasi menggunakan xilena dan sabun cuci piring konsentrasi 0,5%, 1,0%, 1,5%, dan 1,7%. Jenis penelitian eksperimen sungguhan dengan rancangan Posttest only control group design. Hasil penelitian kualitas pewarnaan Hematoksilin Eosin yang dideparafinasi menggunakan xilena dan sabun cuci piring 0,5%, 1,0%, 1,5%, dan 1,7% didapatkan hasil pewarnaan yang baik dengan persentase berturut-turut 100%, 64%, 68%, 88%, dan 88%. Disimpulkan bahwa terdapat perbedaan bermakna kualitas pewarnaan Hematoksilin Eosin yang dideparafinasi menggunakan xilena dan sabun cuci piring konsentrasi 0,5%, 1,0%, 1,5%, dan 1,7%. Kelompok konsentrasi yang memiliki perbedaan bermakna antara sabun cuci piring dengan xilena yaitu konsentrasi 0,5% dan 1,0%, sedangkan pada konsentrasi 1,5% dan 1,7% tidak terdapat perbedaan bermakna atau memiliki kualitas yang sama berdasarkan uji statistik. Disarankan penggunaan sabun cuci piring konsentrasi 1,5% dan 1,7% sebagai alternatif pengganti xilena pada tahap deparafinasi.
Korelasi Antara Kadar Interleukin-6 (IL-6) Dengan Nilai Laju Endap Darah (LED) Pada Pasien TB Paru Di Puskesmas Kecamatan Buleleng Provinsi Bali Dwi Megarani, Ida Ayu Komang
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.20441

Abstract

Tuberkulosis adalah penyakit menular langsung yang disebabkan oleh kuman Mycrobacterium tuberculosis. Sitokin pro inflamasi yang berperan dalam proses infeksi MTB salah satu nya adalah interleukin-6 (IL-6), yang berperan penting dalam respons fase akut dan transisi dari peradangan akut ke kronik, peningkatan protein fase akut yang meningkatkan pembentukan rouleaux sehingga akan meningkatkan kecepatan laju endap darah (Pagana, 2013).Jenis penelitian ini adalah penelitian observasional analitik, dengan rancangan cross sectional.Penelitian ini dilakukan di Puskesmas 1, 2, dan 3 Kecamatan Buleleng, Provinsi Bali, pada bulan April 2023 - Mei 2023, dengan jumlah sampel 30 pasien. Distribusi frekuensi hasil pemeriksaan Kadar IL-6 100% meningkat ≥ 4 ng/L, menggunakan metode ELISA. Distribusi frekuensi hasil pemeriksaan nilai LED normal (3,3%) dan 14 pasien memiliki nilai diatas normal (46,7%) pada laki-laki. Pada perempuan terdapat 7 pasien dengan nilai LED normal (23,3%) dan 8 pasien memiliki nilai LED diatas normal (73,3%), menggunakan metode westergreen. Berdasarkan hasil uji kolerasi nonparametrik Spearman didapatkan nilai signifikansi (p) 0,020 dan nilai koefisien korelasi (r) 0,422. Rata-rata hasil pemeriksaan kadar Interleukin-6 pada pasien Tb Paru adalah 27,066, rata-rata hasil pemeriksaan nilai LED adalah 39,066 Terdapat korelasi antara Interleukin-6 dengan nilai Laju Endap Darah pada pasien Tb (p) 0,020. Kata kunci : Tb Paru, Interleukin-6, dan LED
Korelasi Kadar HbA1c Dengan Kadar Kreatinin Pada Pasien Diabetes Melitus Kronis Ahat, Frederikus
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.20442

Abstract

Uncontrolled diabetes mellitus will lead to various chronic complications, both microvascular and macrovascular complications. HbA1c examination is effective for long-term monitoring of blood glucose for people with diabetes mellitus. A high HbA1c value is used as a measure of the development of complications of diabetes mellitus. Diabetic nephropathy is the most common complication of diabetes mellitus which can end in kidney failure resulting in damage to the glomeruli. Indications to determine kidney function impairment is creatinine examination. This study aims to determine the correlation between HbA1c levels and creatinine levels in patients with chronic diabetes mellitus at the Clinical Pathology Laboratory of the Bajawa Regional General Hospital. Analytical observational research with an approach cros- sectional using techniques purposive sampling 35 chronic DM patients. Test results who squares shows that the value of p = (0.687) > α (0.05), thus it can be concluded that there is no relationship between HbA1c levels and Creatinine levels in patients with chronic diabetes mellitus. Keywords: HbA1c, Creatinine, Diabetes Mellitus.
EKSTRAK DAUN JATI MUDA UNTUK PEWARNA PEMERIKSAAN SEDIMEN URINE Doni Setiawan; Atun Farihatun; Ary Nurmalasari; Euis Tia Istianah; Mujahidah Basarang; Ellies TS Maulidiyanti2
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.20647

Abstract

Urinalysis examination is needed to find information and determine the condition of the kidneys, urinary tract or other organs such as the liver, pancreas, and bile ducts. A urinary sediment examination aims to see organic and inorganic elements in the urine by preparing using Sternheimer Malbin stain reagents. Sternheimer Malbin stain has the disadvantage that it is a synthetic chemical. Stain from natural ingredients becomes a solution as an alternative to reduce the use of synthetic chemicals. Natural stains with the same characteristics and widely tested for bacterial and fungal stains are natural stains from teak leaf extract. Young leaf extract contains anthocyanins in red colour. This study aimed to determine the ability of ethanol extract from young leaves as a natural stain for urine sediment examination. The research method is an experiment with the Static Group Comparison research design. The population and samples in this study are teak leaves determined at the Galuh University Laboratory of the Biology Study Program with the Latin name (Tectona grandis L.f.). The results of the Fisher Exact test obtained a p (value) <0.050, so there was no significant difference between sediments without stain, Sternheimer Malbin, and ethanol extract of young leaves in colouring urine sediments. The conclusion is that the dye of ethanol extract from young teak leaves effectively colours the components of urine sediment, namely epithelial cells, leukocyte cells, and erythrocyte cells with red colour. Keywords      : Anthocyanins, Alternative Dyes, Sternheimer malbin
EFEKTIFITAS PEMBERIAN HIDROGEL EKSTRAK DAUN PEGAGAN (Centella asiatica) TERHADAP PERTUMBUHAN Staphylococcus aureus Amira Muhtadina R.A; Niken Rositasari; Aprilia Retno Sriwijayanti; Riska Putri Novianty; Farida Mumtazza Alkautsar; Vella Rohmayani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.22024

Abstract

Eksim atau dermatitis atopik, merupakan masalah kulit yang dapat mengganggu penampilan,dan  membuat mereka tidak percaya diri. Hal ini karena eksim dapat menyebabkan gatal pada kulit. Gatal yang digaruk berlebihan akan menyebabkan terjadinya inflamasi, sehingga menyebabkan kulit mudah terkena infeksi. Pengobatan eksim selama ini memanfaatkan terapi non farmakologis dan farmakologis. Hasil penggunaan terapi ini membutuhkan banyak waktu, dan biaya. Oleh sebab itu dibutuhkan pengobatan alternative berupa sediaan hidrogel dari ekstrak daun pegagan (Centella asiatica). Daun pegagan (Centella asiatica) adalah tanaman yang memiliki sifat antimikroba. Kandungan aktif daun pegagan memiliki kemampuan untuk menghentikan pertumbuhan bakteri. Tujuan penelitian ini adalah untuk menentukan konsentrasi ekstrak daun pegagan dan seberapa efektif sediaan hidrogel untuk menghentikan perkembangan Staphylococcus aureus. Ekstraksi daun pegagan menggunakan proses maserasi dengan pelarut etanol. Sementara untuk mengevaluasi sifat antibakteri dilakukan dengan metode sumuran. Ekstrak dibuat dalam konsentrasi 2,5%, 5%, 7,5%, 30%, 50%, 75%, serta perlakuan kontrol positif dengan menggunakan antibiotik Clindamycin. Berdasarkan hasil penelitian tersebut didapatkan  rata-rata diameter zona bening sebesar 15,25 mm. Pada konsentrasi 2,5% mendapatkan zona bening sebesar 4,5 mm, pada konsentrasi 5% mendapatkan zona bening sebesar 5 mm, dan pada konsentrasi 7,5% mendapatkan zona bening sebesar 7 mm. Sedangkan pada perlakuan ke-2 dengan konsentrasi 30%, 50%, 75% mendapatkan hasil 24 mm, 27 mm, dan 27 mm dengan control positif sebesar 24 mm. Berdasarkan hasil tersebut menunjukkan bahwa ekstrak daun pegagan memiliki aktivitas antibakteri terhadap Staphylococcus aureus dengan konsentrasi 75% bersifat sensitive mampu menghambat laju pertumbuhan bakteri Staphylococcus aureus dengan terbentuknya zona bening sebesar 27 mm. Keywords : Eczema, Hydrogel, Gotu Kola Leaf, Staphylococcus aureus
Hubungan Konsumsi Ikan Laut Terhadap Kadar Kolestrol Total Pada Nelayan Di Desa Tamberu Timur Waras Budiman; Ellies Tunjung S. Maulidiyanti; Rahma Widyastuti; Rinza Rahmawati Samsudin
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.23602

Abstract

Konsumsi ikan laut yang mempunyai kandungan yang kaya akan protein dan omega-3 serta berkualitas tinggi dan bagus bagi kesehatan, akan tetapi jika mengkonsumsi secara berlebihan dapat menyebabkan lemak jenuh serta dapat meningkatkan kadar kolesterol yang tinggi. Tiap harinya nelayan menghasilkan ikan, hasil tangkapan itu sebagian dijual dan sebagian dikonsumsi sehari-hari. Berdasarkan hasil wawancara dengan nelayan, mereka bekerja sebagai nelayan dalam waktu lebih dari 5 tahun. Kebiasaan nelayan tersebut yaitu tiap hari mengkonsumsi ikan hasil tangkapan, sebanyak lebih dari 3 kali dalam sehari. Tujuan penelitian ini adalah untuk mengetahui hubungan konsumsi ikan laut dengan kadar kolesterol total pada nelayan di desa tamberu timur. Metodeyang digunakan yaitu metode kuantitatif dengan jenis penelitian observasional analitik dengan desain cross sectional. Populasi yang dalam penelitian ini adalah nelayan di Desa Tamberu Timur. Teknik sampling yang digunakan pada penelitian ini adalah menggunakan probability sampling didapatkan 33 responden yang memenuhi syarat kriteria inklusi dan eksklusi. Hasil yang didapat kadar kolesterol yang tinggi yaitu terdapat 20 responden (60.7%), sedangkan kadar kolesterol yang normal sebanyak 13 orang (39.3%). Setelah dilakukan analisis data dengan SPSS didapatkan hasil kadar kolesterol sebesar 0,005< 0,05 sehingga dapat disimpulkan bahwa H1 diterima yang berarti terdapat hubungan antara mengkonsumsi ikan laut dengan kadar kolesterol pada nelayan di Desa Tamberu Timur. Kata kunci : Kolestrol total, Konsumsi Ikan laut

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