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BIOTROPIC The Journal of Tropical Biology

biotropic
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Published by Universitas Islam Negeri Sunan Ampel

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Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

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Articles 94 Documents

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Inventarisasi Tumbuhan Pionir Lahan Bekas Tambang Kapur Di Kecamatan Rengel Kabupaten Tuban Jawa Timur Riska Andriani; Hesti Kurniahu; Sriwulan Sriwulan
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Environmental damage included the limestone mining process would remove the topsoil layer, reduce fertility, reduce microbial diversity and eliminate the vegetation that growth above it. Naturally, pioneer plants could grow to replace lost plants. Pioneer plants can be used as a reference for land recovery because these plants could grow on damaged land because they are associated with Plant Growth Promoting Rhizobacteria (PGPR). In addition, the presence of pioneer plants could inhibit soil erosion due to its root ability to hold the soil from scouring water. This study aimed to determine the characteristics of the initial natural succession process, namely the pioneer post-limestone mining community. This research was conducted by purposive random sampling method at three mining locations, namely Banjaragung Village, 5 years after mine, Maibit Village, 8 years after mine, and Rengel Village, 7 years after mine. Samples were taken for each of the 10 plots of 1x1 m size. The results of this study obtained 5 types of plants with the highest INP, namely: Lamium barbatum, Chromolena odoratum, Tridax procumbens, Waltheria indica, and Digitaria sanguinalis. While the highest diversity index is in the Village of Maibit, Rengel Sub-District, Tuban Regency after 8 years post-mining, which is 2.54.

Toksisitas Ekstrak n-heksana Serbuk Gergaji Kayu Sengon (Albizia falcataria l. Forberg) terhadap Mortalitas Serangga Penggerek Buah Kopi (Hypothenemus hampei ferr.) (Scolytidae: Coleoptera) Purwatiningsih Purwatiningsih; Firna Putri Mandasari; Susantin Fajariyah
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Research on the toxicity of n-hexane extract of Albizia falcataria sawdust against Hypothenemus hampei has been conducted. The method of research was contact method. The concentrations used in the experiment were control (aquadest); 0.25%; 0.5%; 1%; 2%; and 4%. Every concentration was sprayed on the female imago. Observations were performed 120 hours after treatment. The results showed that there was an effect of the length of observation time and concentration on the mortality of H. hampei (GLM Test α= 5%). The time of observation and concentration of A. falcataria sawdust n-hexane extract has positive correlation on H. hampei mortality. Therefore, the longer the treatment time, the more H. hampei mortality is increased. The mortality of H. hampei also increased with increasing concentration given. At a concentration of 4%, extract of A. falcataria sawdust has high toxicity which causes the highest mortality (> 92%).

Taxonomy of Crustose Lichens in The Forest of Tahura R. Soeryo, Batu, East Java Miftahul Jannah; Ludmilla Fitri Untari
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Lichen is a symbiotic organism consisting of a photobiont (green algae or cyanobacterium) and a mycobiont (fungal). A taxonomic study of the crustose lichens in the forest of TAHURA R. Soeryohad been conducted based on morphological, anatomical, and chemical characters. In this research, we used the method of descriptive exploration to identify the crustose lichens and to study the distribution of the species in the forest of TAHURA R. Soeryo. Twenty species of crustose lichens with one unidentified species and one specimen identified to the group of family are reported. They are Graphina, Graphis, Phaeographis, Pertusaria, Pachyphiale, Phlyctis, Lepraria, Lecanora, Lecania, Cyphellium, and Megalospora. This research also found the new record species of Megalospora kalbii in Java. Crustose lichens are found in the areas with high light intensity of ≥ 1000 lux, average humidity of ≤ 90%, average temperature of18-190C, and at altitudes of ±1640 dpl. Graphina ruiziana could only be found at the altitude of ± 1780 dpl. The identification key to its species and distributions of other species are presented also in the article.

Karakterisasi Kromosom Spesies Anggota Familia Solanaceae Ganies Riza Aristya; Chalvia Zuyyina; Dea Febiansi; Rifa Ayuningsih; Kusnah Dian Prasiwi; Triska Ayu Nurwijayanti; Uswatun Mujahidah; Bartolomius Renaldy
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Indonesia is known as a country rich in various agricultural and plantation products, including vegetables. One effort to improve quality of the production by identifying and characterizing chromosomes. The purpose study to characterize number, shape and size of the chromosomes in Solanaceae family. The study using modified Squash method. Chromosome preparation including fixation, maceration, staining, and analyzed using Image Raster 3 application, then Corel Draw X6. The results showed study to characterize the number, shape and size in the Capsicum annum L. Tm 999 (2n=20m+4sm and 2,41 s/d 9,99 µm) then Capsicum frutescens L. Kencana (2n=16m+8sm and 3,12 s/d 10,49 µm). In Solanum lycopersicum Or Diana and Marta (2n=16m+8sm and 3,12 s/d 10,49 µm) then (2n=22m+2sm and 2,65 s/d 7,28 μm). In Lycopersicon esculentum Mill. Juliet and Tropical Ruby (2n=18m+6sm and 0,77 s/d 1,81 μm) then (2n=20m+4sm and 0,39 s/d 0,94 μm). Solanum melongena L. Or Valerie and Mustang (2n=24m and 0.29 s/d 0.87 μm) then (2n=22m+2sm and 0,5 s/d 1.28 μm). In Solanum melongena L. Kania and Pulus (2n=20m+4sm and 0.64 s/d 3.43 μm) then (2n=16m+8sm and 0.84 s/d 2.51 μm). In Solanum melongena L. Jeno and Planet Hijau (2n=18m+6sm and 0.65 s/d 2.423 μm) then (2n=14m+10sm and 0.46 s/d 1.73 μm). In Capsicum annum var. Grossum L. Red Star and Purple Star (2n=22m+2sm and 3.19 s/d 8.49 μm) then (2n=24m and 5.5 s/d 11.92 μm).

Keanekaragaman Tumbuhan Berkhasiat Obat Pada Suku Noaulu Di Pulau Seram, Maluku Sarmawaty Kotala; Nirmala Fitria Firdhausi
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Noaulu an indigenous tribe of Seram Island who live in the Sepa Village of Amahai District, Maluku. This tribe still use forest products to fulfill their needs, including medication. Therefore, this study intends to know the kinds of medicinal plants used by the Noaulu Tribe and the types of diseases that can be treated. Collecting data in this study using survey method and an interview method with the public interest. Determination of the respondents was using purposive sampling method. Inventory of medicinal plants was done by a survey method survey based respondent information. The result of the research shows that there are 31 medicinal plants from 22 families. They are able to cure 20 diseases. The plants which are predominantly used are from Zingiberaceae family. Leaves of the plant are mostly used as the drugs.

Dragonfly Diversity in Cibodas Botanical Garden in West Java Susanti Murwitaningsih; Agus Pambudi Dharma; Maryanti Setyaningsih; Yati Nurlaeni
Biotropic : The Journal of Tropical Biology Vol. 3 No. 1 (2019): Biotropic, Volume 3, Nomor 1, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

The dragonfly (Odonata) is a flying insect that plays an important role in the food chain and acts as a bioindicator of water health. This is due to its biological characteristics which include spending most of its life stages in the water and preying on smaller insects. This study is aimed to investigate the level of dragonfly diversity in Cibodas Botanical Garden, West Java. The method used in this research was direct observation using catch and release technique. The research was carried out from May to July 2018. A total of 1,510 individuals belonging to eight dragonfly species were found in the four sampling locations. These were divided into two Sub-Orders: 1) Anisoptera (Orthetrum pruinosum, Pantala flavences, Orthetrum sabina, Neurothemis fluctuans, Neurothemis terminata, and Orthetrum glaucum); and 2) Zygoptera (Ischnura senegalensis, dan Ceoliccia membranipes). The highest values of species diversity index (H') and evenness index (E) were found in Ciismun Waterfall (1,45 and 0,88, respectively). The dominance index (D) in the four sampling sites ranged between 0.25 - 0.32. The highest species richness index (R) was found in Sakura Garden (0,99).

Identification of Bryophytes in Jumog Waterfall, Karanganyar, Central Java, Indonesia Yasir Sidiq; Alanindra Saputra; Siti Kartika Sari
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Indonesia, as a mega-biodiversity country, has a high diversity of bryophytes (Liverworts, Hornworts, and Mosses). In addition, a waterfall is one of the most optimum places for bryophytes to life. One of many waterfalls in Indonesia is Jumog Waterfall. It is located in Karanganyar Regency, Central Java. This study aimed to know the diversity of bryophytes in Jumog Waterfall, identify the species, and analyze the genetic relationship through the species based on morphological characters. The explorative method was used to collect the samples. Identification of species was done by comparing samples to the handbook of identification. The results were; (1) there were fifteen species that found in Jumog Waterfall; (2) The highest similarity among species was 90%. They were grouped in genus Fissidentales, Order Dicranales, and Order Hypnobryales. Then, The lowest similarity was 30.8%. They were between Funaria hygrometrica and Riccia fliutans. It distinguished liverwort, hornwort, and mosses

Pengaruh Ekstrak Kulit Durian (Durio zibethinus) terhadap Pertumbuhan Jamur Patogen pada tanaman Kentang (Phytophthora infestans) Secara In Vitro Lailatus Sa'diyah; Rizki Dwi Putri; Alfina Zahwa; Guntur Trimulyono
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak kulit buah durian terhadap pertumbuhan jamur patogen pada tanaman kentang secara in vitro. Metode yang digunakan adalah eskperimental dengan rancangan acak lengkap (RAL) dengan variabel manipulasi berupa dosis ekstrak yaitu 15%, 25%, 50% dan 75%, kontrol positif menggunakan Dithane M-45 dan kontrol negatif menggunakan aquadest. Variabel respon yang diamati berupa diameter pertumbuhan jamur patogen tanaman kentang (Phytophthora infestans). Prosedur penelitian meliputi pembuatan ekstrak kulit durian dilanjutkan uji aktifitas antijamur. Data dianalisis dengan uji ANOVA dan dilanjutkan dengan uji BNT (Beda Nyata Terkecil). Hasil penelitian yang telah dilakukan menunjukkan bahwa semakin tinggi konsentrasi ekstrak maka daya hambat pertumbuhan jamur juga semakin besar. Daya hambat ekstrak terhadap pertumbuhan jamur pada konsentrasi ekstrak 50% merupakan yang tertinggi dibandingkan konsentrasi ekstrak lainnya yaitu sebesar 77,3%. Berdasarkan uji anova diperoleh nilai signifikasi yaitu 0,000 (<0,05) dan nilai F sebesar 570,542 artinya ada pengaruh pemberian ekstrak kulit durian terhadap pertumbuhan isolat jamur patogen pada tanaman kentang, sedangkan berdasarkan uji BNT diperoleh hasil bahwa masing-masing konsentrasi berbeda nyata dalam menghambat pertumbuhan isolat jamur patogen pada kentang. Sehingga dapat ditarik kesimpulan bahwa pemberian ekstrak kulit durian dapat menghambat pertumbuhan jamur patogen pada tanaman kentang secara in vitro.

Profil Senyawa Bioaktif Ekstrak Kalus Biji Jeruk Purut (Citrus hystrix DC.) Pasca Induksi Metil Jasmonat Yeni Avidhatul Husnah; Ari Indrianto; Woro A.S Tunjung
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Kaffir lime (Citrus hystrix DC.) is an alternative source of potential and relatively safe anticancer compounds as it contains bioactive components. Previous study has shown that the extract of kaffir lime leaves contains terpenoids and potentially as anticancer. However, the content of bioactive compounds may vary due to environmental factors. Callus culture is an alternative method of induction of bioactive compound content with a controlled environment. Previous study has succeeded in inducing callus from kaffir lime seed explants produce the ideal callus with a crumb / friable texture. Methyl jasmonate is reported to induce the production of secondary metabolite compounds. Elicitation with methyl jasmonate is one of the strategy to increase terpenoids from cultured cells Objective of this study is anlyze the using of methyl jasmonate as elicitor.to increase bioactive compounds inkaffir lime cell suspension. Callus induced from seed explants age 40 days were transferred into liquid medium for the initiation of suspension culture. Subculture is done when the age of culture reaches 21 days then measured thegrowth curve o f cell supension and concentration of sucrose in medium. After cells reached log phse in 12 days then be treated with methyl jasmonic for 8 days. The cell suspension then beextracted with ethyl acetate by maceration method. The content of bioactive compounds in the extract was anlyzed with GC-MS. Suspension of lime lime cells C. hystrix, without treatment yields fatty acid compounds. Bioactive compounds produced by suspended cell suspension after methyl jasmonate elicitation is germacrene A, myrcene, alpha-terpineol, geranyl acetate, trans-caryophyllene, delta-guaiene and several types of ester fatty acids.

Perubahan Kualitas Sperma Mencit (Mus musculus) Yang Terpapar Asap Rokok Elektrik Andita Ayu Mandasari; Siti Nur Asiyah; Kurnia Lintang
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Smoking is one of the habits that can affect human health. One type of cigarette that is currently the choice is electric cigarettes. The e-cigarette content includes nicotine, propylene glycol, flavorings and glycerin. The purpose of this study was to determine the effect of exposure to e-cigarette smoke on mice strain balb / c with the parameters of the number of spermatozoa. This study used 15 male mice which were 8-10 weeks old and weighed 25-30 grams of male mice which were divided into 3 treatment groups namely control group, treatment group one and treatment group two. In the control group no exposure to cigarette smoke was carried out while in the treatment group one was exposed to cigarette smoke in mice with a dose of 0 mg nicotine and the second treatment group was exposed to e-cigarette smoke at a dose of 18 mg nicotine. Electric cigarette smoke exposure is carried out for 30 days using a suction device from a syringe. The exposure of cigarette smoke is carried out every day with successive doses of 20 times as much as 3 times with a time lag of 10 minutes (5 minutes the chamber is closed and 5 minutes of the chamber is opened). The results showed that the average number of spermatozoa in the control group was 16.816 million / ml. The average number of spermatozoa in treatment one was 10.432 million / ml and the average number of spermatozoa in treatment two was 5.234 million / ml. This shows that exposure to cigarette smoke can reduce the number of spermatozoa from the control group to the first treatment by 42.56% and the second treatment shows a decrease from the first group by 34.65%.

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