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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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The Relationship of Body Length and Ratio Pappilla with Sex in Gobi Fish (Sicyopterus macrostetholepis) Sari, Rona Taula; Zakaria, Indra Junaidi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8747

Abstract

Research about the relationship of body length and ratio papilla with sex in goby fish (S. macrostetholepis Blkr.) has been done at Animal Structure and Developmental Laboratory, Biologi Department, Faculty of Matematics and Natural Sciences, Andalas University, Padang, which purposed to analyse the relationship of body length and ratio papilla with sex of goby fish (S. macrostetholepis Blkr.). The samples were taken in wild stream area at Batangkuranji river, Padang City. This research used descriptive method and data were analyzed by qualitatively and quantitatively. The results of investigation showed that in several goby fishes (S. macrostetholepis Blkr.) with different sex had the same of body length and the same of ratio papilla. So, there was not relationship between of body length and ratio papilla with sex. Goby fishes (S. macrostetholepis Blkr.) it belongs to the hermaphrodite protogini, which the androgynous young females, while in adulthood, it would change sex to male. The results of this study are expected to add to the treasures of knowledge and information about reproductive gobies (S. macrostetholepis Blkr.) in the preservation and development of fish farming.
Isolation of Thermophilic Bacteria from Bora Hot Springs in Central Sulawesi Ifandi, Slamet; Alwi, Muh
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14905

Abstract

Thermophilic bacteria can survive at high temperature, in which hot spring is one of its habitats. Indonesia has many hot springs with potential as a habitat for thermophilic bacteria. The purpose of this study was to obtain isolates thermophilic bacteria from Bora hotspring located in Central Sulawesi. This study applied a descriptive-observational study design, characteristics of bacterial properties identified using conventional methods according to the Bergey’s Manual of Determinative Bacteriology. The study was conducted in 3 stages. The first stage was bacteria cultivation on the appropriate media, followed by stage of isolated and the last stage by identified characteristics of thermophilic bacteria which included microscopic and macroscopic morphology, Physiological and biochemistry test. The results of the isolates indicted 4 representative isolated of thermophilic bacteria from Bora Hot Spring namely TM022, TM023, TM024, TM026. The bacteria isolates obtained were bacillus, coccus and Gram negative and positive, while the physiological test of all isolates were able to grow and showed changes in the medium. This study is useful in providing characteristic of indigenous thermophilic bacteria isolates that produces thermostable enzymes.
Struktur Mikroanatomi Insang Ikan Bandeng di Tambak Wilayah Tapak Kelurahan Tugurejo Kecamatan Tugu Semarang Susanah, Utari Ani; Santosa, Kukuh; Utami, Nur Rahayu
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.3925

Abstract

Berbagai macam kegiatan industri dan teknologi di wilayah Tapak Kelurahan Tugurejo Kecamatan Tugu yang ada saat ini apabila tidak disertai dengan program pengelolaan limbah yang baik akan memungkinkan terjadinya pencemaran air. Ikan sebagai salah satu biota air dapat dijadikan sebagai suatu indikator tingkat pencemaran yang terjadi di dalam perairan. Tujuan penelitian ini adalah untuk mengetahui kondisi air tambak dan kerusakan struktur mikroanatomi insang ikan bandeng yang ada di tambak wilayah Tapak Kelurahan Tugurejo Kecamatan Tugu Semarang. Penelitian dilakukan di tambak wilayah Tapak Kelurahan Tugurejo Kecamatan Tugu Semarang, laboratorium BBTPPI Semarang dan laboratorium kedokteran hewan UGM. Pengambilan data menggunakan purposive random sampling. Analisis data dalam penelitian ini adalah deskriptif kualitatif. Hasil penelitian terhadap faktor lingkungan ikan bandeng masih berada dalam ambang batas yang ditentukan namun hasil pengamatan struktur mikroanatomi insang menunjukkan terdapat kerusakan struktur mikroanatomi berupa edema, hyperplasia, atrofi, fusi lamella, curling dan nekrosis. Hal ini diduga karena zat toksik lain yang ada diperairan namun tidak terukur dalam penelitian. Disimpulkan bahwa pencemaran air tambak di wilayah Tapak Kelurahan Tugurejo Kecamatan Tugu Semarang masih berada pada pencemaran tingkat awal. Pada hasil pengamatan struktur mikroanatomi insang ikan bandeng kerusakan yang terjadi pada jaringan disebabkan lebih karena kerusakan struktur mikroanatominya.A wide variety of industrial and technological activities in Tapak Area Tugurejo Village Tugu District could result in water pollution if they are not accompanied by a good waste management program. Fish as one of aquatic biota can be used as an indicator of the level of pollution in the waters. The aim of this research is to determine the condition of pond water and mikroanatomi structural damage of milkfish gills in Tapak pond. Data retrieved using purposive random sampling. Data analysis is qualitative descriptive. The result of environmental factors milkfish showed that environmental factors milkfish still in the threshold which is determined, but observations mikroanatomi gill structure showed that there are mikroanatomi structural damage in the form of edema, hyperplasia, atrophy, fusion lamella, curling, and necrosis. It is thought to be caused by other toxic substances that exist in waters, but it was not measured in the research. It was concluded that water pollution in Tapak pond still at the initial level of pollution. In observations milkfish gill structure mikroanatomi showed that tissue damage caused by damage structure its self.
EFEK PERENDAMAN INFUSA DAUN SALAM (Syzygium polyanthum) TERHADAP KUALITAS DAGING AYAM POSTMORTEM Agustina, Feri Dwi; Widiyaningrum, Priyantini; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2271

Abstract

Tujuan penelitian ini untuk mengevaluasi efek perendaman daging ayam broiler dalam konsentrasi infusa daun salam yang berbeda. Penelitian menggunakan desain faktorial dengan 3 taraf konsentrasi infusa (0%; 5%; dan 10%) serta dua waktu pengamatan yaitu 0 dan 8 jam setelah perlakuan perendaman. Parameter yang diukur meliputi rata-rata bilangan peroksida, pH, kadar air dan water holding capacity (WHC). Data dianalisis menggunakan analisis varian dan uji perbedaan akibat perlakuan dilakukan dengan uji beda nyata terkecil. Hasil penelitian menunjukkan bahwa pengukuran pH dan WHC sesaat setelah perlakuan memperlihatkan perbedaan nyata (P<0,05), sementara bilangan peroksida dan kadar air tidak berbeda nyata. Pada 8 jam setelah perendaman, terlihat bahwa nilai peroksida, pH, kadar air dan WHC menunjukkan perbedaan nyata. Penelitian ini menyimpulkan bahwa infusa daun salam mampu memperlambat laju bilangan peroksida, memperlambat penurunan pH dan memperlambat penurunan WHC.The aim of this study was to evaluate the effects of the immersion of chicken meat in the bayleaf extract and the time of measurement on the quality of the meat. This was a factorial experiment with three levels of bayleaf extract concentrations (0%, 5% and 10%), and two time of measurement (at 0 and 8 hours after the treatment). The parameters observed were the average peroxide value, the pH, the water content and the water holding capacity. The data were analyzed using ANOVA and followed by the least significant difference test for any significant difference. The results showed that the pH and the water holding capacity were significantly different (P<0.05), whereas the peroxide value and the water content were not significantly different. Nevertheless, at eight hours after the immersion it was seen that the peroxide value, the pH, the water content and the water holding capacity were significantly different. It was concluded that the extract of bayleaves might slower the rate of the peroxide value, could decrease the pH, and decrease the water holding capacity.
Keanekaragaman Genetika Pisang Bergenom B Berdasarkan Penanda Mikrosatelit Windarti, Windarti
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.34

Abstract

The morphology and isozyme marker was not enough to reveal banana genetic diversity. So, it needs molecular analysis. The purpose of this research is to know the genetic diversity of banana with B genome using microsatelite marker. Forty bananas with B genom from Yogyakarta Agriculture Service and Animal Husbandary Service and also from Tropical Fruit Centre of Bogor Agriculture Institute were amplified using 6 loci (MaCIR327b, MaCIR108, Ma-3-139, Ma-3-90, Ma-1-17, and Ma-1-27). The diversity were identified with allele amount, genotype, observe heterozigosity, and filogeny. There are eight allele per locus average from six microsatelites. MaCIR108 produced the highest allele amount, there are 13 alleles. The research identified 75 genotypes. MaCIR327b, MaCIR108, Ma-3-139, Ma-3-90, Ma-1-17, and Ma-1-27, respectively, produced 8, 20, 13, 14, 15, and 5 alleles. The Ho average is quite high, there are 0,77. The similarity coefficient is between 0,597 – 1,00. MaCIR108 and Ma-3-90 can be used to identify banana group genom. As a group genom characteristic, Ma-1-17 needs more research. Identification result between morphology and microsatelite marker were different. Based on morphology marker, Sri Wulan and Byar cultivar were ABB, and Ketip Gunung Sari were AAB, but based on microsatelite marker Sri Wulan and Byar cultivar were AAB, while Ketip Gunung Sari has not B on its genom. Keywords: genetic diversity, banana, microsatelit.
Population Dynamics of Pometia for The Period of Post-Selective Logging in Tropical Rainforest, Southern Papua, Indonesia Murdjoko, Agustinus; Marsono, Djoko; Sadono, Ronggo; Hadisusanto, Suwarno
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6309

Abstract

Tropical rainforest changed in term of structure, composition and population, resulting from logging. One of target trees during logging is Pometia. Our goals of this research were to answer that (1) How were survival, growth, fecundity and population growth rate (?) of Pometia within remnant stands?; (2) what were driving factors to explain population dynamics of Pometia within logged forest?. Data were derived from three hectares-permanent sample plot (PSP) in logged forest of PT Tunas Timber Lestari. Integral Projection Models (IPMs) were applied to analyze population dynamics. The study showed dynamically that probability of the individuals of Pometia survived in this area did not significantly differ among their size, while stands showed the significant difference between small and large individuals. The growth of both individuals of Pometia and stands varied significantly in different size. The probability of fecundity both Pometia and stands were significantly different among size. The population of Pometia has been increasing for about a decade as the population of all stands was growing as well. Moreover, the regular documentation of species composition and structure before and after logging is necessary as a control of biodiversity. Therefore, a proper protocol to monitor the biodiversity should be designed by government and disseminated it to logging companies. How to CiteMurdjoko, A., Marsono, D., Sadono, R. & Hadisusanto, S. (2016). Population Dynamics of Pometia for The Period of Post-Selective Logging in Tropical Rainforest, Southern Papua, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 8(3), 321-330.
Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Putri, Intan Permata; Savira, Nadyatul Ilma Indah; Winarni, Dwi; Suhargo, Listijani; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
Diversity of The Ornate Lorikeet (Trichoglossus ornatus) Birds Based on Mitochondrial DNA Protein Coding Gene Astuti, Dwi; Prijono, Siti Nuramaliati
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13501

Abstract

Ornate lorikeet (Trichoglossus ornatus) is an endemic bird in Sulawesi. Endemism is one of the factors in declining bird’s population. In the case of the birds conservation programme, information about gene diversity is important for basic strategy. Mitochondrial DNA of animals consists of protein coding genes including ND2 gene. This study informs diversity of the Ornate Lorikeet (Trichoglossus ornatus) birds based on DNA sequences of ND2 gene. DNA total was extracted from blood samples of 21 birds. PCR (Polymerase Chain Reaction) was performed and successfully amplified a single DNA fragment of ND2 gene for all birds. DNA fragments were sequenced and totally 997 base pairs were analyzed. NJ tree was constructed using MEGA5. All DNA sequence data showed that between the birds there were 20  polymorphic (segregating) sites with mean genetic distance was 0.004 ± 0.002 (ranged from 0,000 – 0,008), and had 17 sequence haplotypes (HTor1- HTo17). Haplotype diversity (Hd) was 0.967 ± 0.30387 and nucleotide diversity (Pi) was 0.00439 ± 0.0012. Genetic diversity information could be potential relevance to the breeding management for conservation of the birds.
Characteristics of superior soybean breeding lines tolerance to rust (Phakopsora pachyrhizi Syd.) Inayati, Alfi; Yusnawan, Eriyanto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5081

Abstract

Soybean rust caused by Phakopsora pachyrhizi is one of the most important diseases which limits soybean production. The aim of this study was to evaluate the resistance of 28 superior soybean lines and their tolerance to rust. The study was conducted at a screen house and arranged in a completely randomized design (CRD); three replications. All genotypes tested were artificially inoculated with P. pachyrhizi, and a set of un-inoculated genotypes was planted as a comparison. Number of pustules was recorded weekly, and resistant criteria was rated based on the International working group on soybean rust IWGSR method. Lesion color (LC), sporulation level (SL), number of uredia (NoU), frequency of pustule which had uredia, and yield were also recorded. Among 28 genotypes tested, only one was categorized as resistant and 2 genotypes were susceptible. Resistant genotypes had few pustules, lower AUDPC values, low disease severity, and Reddish Brown lesion type. Soybean rust affected yield components, i.e. number of intact pods and yield per plant. Yield loses due to rust in this study varied from 5-89%, and the average was 51%. The set of lines from Tanggamus pedigree showed more resistant to rust but less tolerant compared to Sinabung pedigree.How to CiteInayati, A., & Yusnawan, E. (2016). Characteristics of superior soybean breeding lines tolerancet to rust (Phakopsora pachyrhizi Syd.). Biosaintifika: Journal of Biology & Biology Education, 8(1), 47-55.
Transformasi ?-Pinena dengan Bakteri Pseudomonas aeruginosa ATCC 25923 Wijayati, Nanik; Astutiningsih, Christina; Mulyati, Suci
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2931

Abstract

Indonesia adalah Negara utama yang memproduksi minyak atsiri di dunia. Minyak terpentin adalah minyak atsiri yang dihasilkan dari destilasi getah pinus Pinus merkusi J ungh. Et. De. Vr. Tujuan penelitian ini adalah untuk meningkatkan nilai minyak terpentin dengan mengubah kandungan utamanya, ?-pinena menjadi senyawa baru menggunakan P. Aeruginosa dalam metode mikrobiologi. Minyak terpentin diambil dari Perhutani Laboratorium Jawa Tengah, dibuat dengan seri konsentrasi 0,5%, 1%, 2%, dan 4%. Minyak terpentin diinokulasi dalam suspensi P. areuginosa selama 48 jam pada suhu kamar (25-28oC). Hasilnya diekstraksi menggunakan dietil eter. Filtrat Terpentin dianalisis menggunakan GCdan IR. Hasil analisis GC menunjukkan puncak baru di konsentrasi 0,5%, 1%, dan 2%, tetapi dalam konsentrasi 4% tidak menunjukkan puncak baru. Hasil IR menunjukkan hidroksil (OH-) dan C-O alkohol. Berdasarkan penelitian ini, dapat disimpulkan bahwa minyak terpentin dapat ditransformasi untuk menjadi senyawa yang mengandung gugus-OH melalui metode mikrobiologi dengan menggunakan bakteri P. aeruginosa.Indonesia is the main producer of essential oil in the world. Turpentine oil is an essential oil which is obtained from pine resin distillation of Pinus merkusi Jungh. et. De.Vr. The aim of this experiment was to increase the value of turpentine oil by changing its main content, i.e. ?-pinene, into a new compound using P. aeruginosa in microbiological method. Turpentine oil was collected from Perhutani Central Java Laboratory, and was made into 0.5%; 1%; 2%; and 4% concentrations and it was inoculated in P. areuginosa suspension for 48 hours in room temperature (25C-280C). The result was extracted using diethylether. The filtrate of turpentine was analyzed using GC and IR. The GC analysis result showed a new peak in 0.5%; 1%; and 2% concentrations, but in the 4% concentration didnt show a new peak. The IR result showed alcohol with hydroxyl (-OH) and CO groups. This experiment concluded that turpentine oil may be transformed using P. aeruginosa in a microbiological method to become a substance containing OH group.

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