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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Identification of Soybean Genotypes for Pod Shattering Resistance Associated with Agronomical and Morphological Characters Krisnawati, Ayda; Adie, Mochammad Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8722

Abstract

A yield loss caused by pod shattering is one of the obstacles to the improvement of soybean productivity in tropical areas. The aim of this study was to identify the resistance of soybean genotypes to pod shattering as affected by agronomical and morphological characters. The field study was conducted in Malang, Indonesia, using 150 soybean genotypes. Data were collected on agronomical traits, the percentage of pod shattering, and pod morphological traits. Identification for shattering resistance was done as per oven dry method. Percentage of pod shattering was ranged from 0 % up to 100 % shattering with a mean of 58.11 %. Pod shattering was found to be negatively correlated with a number of pod per plant, the thickness of the pod, and Y/Z (seed weight and pod weight ratio). The Identification obtained 66 very highly susceptible genotypes, 19 susceptible genotypes, 19 moderate genotypes, 38 resistant genotypes, and 8 very resistant genotypes. Two of eight very resistant genotypes (G511H/Anj//Anj///Anj////Anj-6-11 and G511H/Anj//Anj///Anj////Anj-5-4) have high yield, medium maturity day and large seed size. Those lines could be used as gene donor for soybean varietal improvement for shattering resistance, and recommended to propose as new improved soybean varieties resistant to pod shattering in Indonesia.
Histology Structure of Lymph and Uterus of White Rat (Rattus norvegicus) Given with Nanas Bongsai (Ananas comosus var. microstachys L.) Fitmawati, Fitmawati; Saputra, Agus; Yohanes, Yohanes; Teruna, Hilwan Yuda; Nugraha, Dimas Pramita; Hamidi, Yulis
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13596

Abstract

Nanas Bongsai (Ananas comosus var. microstachys L.) is an amazing herb which is used in traditional medicine by local people of Muara Lembu district as a potion to reduce pain while menstruation period for a women. The present work was designed to investigate its probable side effects on the histopathologic changes in limph and uterus tissues after treated with A. comosus var. microstachys L extract in female white rats. This is an experimental research consisted of five treatments and three repetitions. Treatment composed of two controls (P0 and P+) and given Nanas Bongsai extract with three different dosages. Histology result of lymph show that all treatments given with Nanas Bongsai with three different dosages have different diameter average of white pulp and there is no significant changes with normal control. While observation towards histology structure of endometrium thickness with dosage P1, dosage P2, dosage P3 is 206.333±33.486; 215.667±33.486; 197.667±60.871 respectively. The most thin endometrium layer found in treatment with dosage P3 compared to control P0. From this study showed that there is no toxic effect on uterus and lymph function of of Nanas Bonsai (A. comosus var. microstachys L.) at different doses, so the plant is secure for consumption by the community.
PENGARUH EKSTRAK LIMBAH DAUN TEMBAKAU MADURA TERHADAP AKTIVITAS MAKAN LARVA Spodoptera exigua -, Harwanto; Martono, Edhi; Trisyono, Andi; -, Wahyono
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2262

Abstract

Ekstrak tanaman untuk insektisida nabati tidak hanya berpengaruh terhadap mortalitas saja akan tetapi juga berpengaruh terhadap aktivitas makan serangga. Tujuan pengujian ini adalah untuk mengetahui pengaruh ekstraklimbah daun tembakau madura terhadap aktivitas makan larva instar III Spodoptera exigua. Metode uji ada dua yakni pakan pilihan dan pakan tanpa pilihan. Rancangan yang digunakan adalah rancangan acak lengkap (RAL), dengan 6 perlakuan konsentrasi dan 1 kontrol yang diulang 5 kali. Hasil pengujian menunjukkan bahwa pemaparan ekstrak limbah daun tembakau dengan metode pakan pilihan: (1) berpengaruh nyata terhadap bobot daun yang dikonsumsi pada semua konsentrasi dibandingkan dengan kontrol, (2) bersifat antifeedant terhadap larva S. exigua dengan indeks hambatan makan berkisar 17,51 – 38,12%, sedang ekstrak limbah daun tembakau pada uji pakan tanpa pilihan (3) berpengaruh nyata pada konsentrasi > 0,21% terhadap bobot daun yang dikonsumsi dibandingkan dengan kontrol, dan (4) bersifat antifeedant dengan indeks hambatan makan lebih tinggi (22,87 – 69,39%) daripada dengan pakan pilihan (17,51 – 32,12%). Plants extracts used for botanical insecticides do not only influence the mortality but also the feeding activity of insects. The aim of this test was to determine the activity of the extract of Madura tobacco leaf on the feeding activity of the third instar of beet armyworm Spodoptera exigua. There were two test methods, choice and no-choice feeding tests. The design of each test was a completely randomized design (CRD), with six treatment concentrations and one control, replicated five times. The results showed that the exposure of tobacco leaf extracts with the choice feeding: (1) had significant effect on the leaf weight consumed at all concentration compared to the controls, (2) act as antifeedant against the larvae of S. exigua with the index of food consumption barrier ranging from 17.51 to 38.12%; while the extract of tobacco leaf on the no-choice feeding test: (3) had significantly effect on the weight of leaves consumed compared to the control on the concentrations of > 0.21%, and (4) was antifeedant with the index of food consumption barrier higher (22.87 to 69.39%) than the choice feeding (17.51 to 38.12%).
Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium Ratnaningtyas, Nuniek Ina; Purnomowati, Purnomowati; Purwati, Endang Sri; Septiana, Aisyah Tri; Ekowati, Nuraeni; Supriyadi, Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11512

Abstract

Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate (non-polar) extraction of mycelium of Ganoderma sp. had a potential character as an antioxidant source and performed a better result than from ethanolic (polar) extraction as shown in the IC50 value. Extract from ethyl acetate extraction had an average IC50 value smaller than  from ethanolic extract (581.80 < 1285.67). Extract from ethyl acetate extraction resulted in a higher amount of phenol than that ethanolic extract 29.23 < 57.67. Inhibition percentage of both extracts at 65% was known to occur at concentration of 1000 ppm for ethyl acetate extract and 2000 ppm for ethanolic extract. An important finding was that ethyl acetate can be used as appropriate solvent for extracting antioxidant compound better than ethanolic. In conclusion, the mycelium extract of Ganoderma sp. extracted with ethyl acetate and ethanol as solvent is potential to be used as a source of natural antioxidants. This research result has benefit in developing potency of local resources as herbal resources.
Effect of Turmeric Powder Supplementation to The Age of Sexual Maturity, Physical, and Chemical Quality of The First Japanese Quails (Coturnix japonica) Egg Saraswati, Tyas Rini; Tana, Silvana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4982

Abstract

The experiment was conducted to determine the effect of turmeric powder supplementation to the age of sexual maturity, physical, and chemical quality of the first Japanese quails (Coturnix japonica) egg. Forty five quails were assigned into a completely randomized design with three treatments (levels of turmeric powder, i.e., 0; 54; and 108 mg/quail/day) and each treatment used 15 quails. Turmeric powder supplementation was conducted before sexual maturity. Feed and drinking water provided ad libitum. Observed egg is an egg that was first produced. Parameters measured were the age of sexual maturity, feed intake, body weight, physical qualities which include: weight of egg, long axis, short axis, weight and thickness of shell, yolk index, Haugh unit, egg shell index. Whereas the observed chemical quality were cholesterol, HDL, LDL, protein, vitamin B12, vitamin A in eggs and egg shell calcium levels. The results showed that administration of turmeric powder can accelerate the age of maturity, increasing the levels of protein, HDL, vitamin A and B12 in eggs, decreasing the cholesterol and LDL content in eggs, but did not affect feed intake, physical quality of eggs and egg shell calcium levels. Based on the results of this study, it can be concluded that supplementation of turmeric powder improve the chemical quality of Japanese quail eggs (Coturnix japonica), so it is good for the development of quail embryos as well as for consumption.How to CiteSaraswati, T. R., & Tana, S. (2016). Effect of Turmeric Powder Supplementation To The Age of Sexual Maturity, Physical, and Chemical Quality of The First Japanese Quails (Coturnix japonica) Egg. Biosaintifika: Journal of Biology & Biology Education, 8(1), 18-24.
UJI POTENSI Gliocladium sp TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN TOMAT Herlina, Lina
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2747

Abstract

AbstrakMikroorganisme tanah seperti Gliocladium sp dapat bertindak sebagai dekomposer dan juga sebagai agen pengendali hayati patogen tanaman hal ini memberikan harapan untuk mengurangi penggunaan pupuk dan fungisida sintetik. Tujuan penelitian untuk menguji potensi biofertililzer Gliocladium sp terhadap pertumbuhan dan produksi buah tomat. Variabel bebas yaitu bioferlizer Gliocladium sp dengan dosis (g/tanaman) 0,50, 100, 150, dan 200 .Variabel tergantung adalah pertumbuhan dan produksi tanaman tomat. Parameter pertumbuhan yang diukur adalah tinggi tanaman, kandungan klorofil a dan b. Parameter hasil tanaman yang diukur adalah berat buah setelah panen. Hasil uji Anava satu jalan menunjukkan bahwa pemberian Gliocladium sp perpengaruh terhadap tinggi tanaman, kandungan klorofil a dan klorofil b, berat tomat . Hasil uji lanjut Duncan menunjukkan bahwa dosis 100, g 150 g dan 200 g tidak berbeda signifikan terhadap tinggi tanaman, dosis 150g dan 200 g tidak berbedan nyata dalam pembentukan klorofil a dan klorofil b. Pemberian Gliocladium dosis 150g paling tinggi terhadap berat buah. Simpulan dalam penelitian ini bahwa pemberian Gliokompos sp berpengaruh terhadap pertumbuhan dan produksi buah tomat.AbstractSoil microorganisms such as Gliocladium sp. can play role in the decomposition and can also become the biological control of pathogenic plants. The animal is potentially reduce the use of fertilizers and synthetic fungidid. The aim of the study was to test the potential effect of biofertililzer Gliocladium sp. on the growth and production of tomatoes. The independent variables was biofertilizer Gliocladium sp. at various dosages (g/plant) of 0, 50, 100, 150, dan 200. The dependent variables were the growth and the production of tomatoes. The growth parameter measured were plant height, as well as a and b chlorophyl contents. The crop parameter was the fruit weight post-harvest. A one-way anova showed that Gliocladium sp. affected the plant height, the a and b chlorophyl contents, and the tomato weight. The Duncans post-hoc test showed that there was no significant difference on plant height among 100g, 150g and 200g treatment dosages, and 150g and 200g treatment dosages do not affect the formation of a and b chlorophyl. The Gliocladium dosage of 150g was the strongest in influencing the fruit weight. It was concluded that the administration of Gliokompos sp. affects the growth and production of tomatoes.
Various Plants of Traditional Rituals: Ethnobotanical Research Among The Baduy Community Iskandar, Johan; Iskandar, Budiawati S.
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8117

Abstract

On the basis of recent studies in many places of developing countries it has been revealed that the practices of conservation of biodiversity has strongly determined by traditional ecological knowledge, and beliefs or cosmos. The aim of the study namely to elucidate; (1) some traditional rituals in the swidden management system of the Baduy community; (2) various plants that have been used for performing some rituals in the swidden management system of the Baduy community; and (3) some functions of various plants that have been used in the rituals of the swidden management system of the Baduy community. A qualitative method with ethnobotanical approach was applied in this study. The result of study shows that 9 kinds of the traditional rituals that have been predominantly undertaken by the Baduy community in the management of swidden farming system. At least 50 plant species representing 28 families have been used for those performing traditional rituals. The main function of plants in the rituals is considered as the symbolic meaning and rational function. The result of study has been considered very important that the traditional ecological knowledge and beliefs must be considered to conserve biological diversity.
Analysis of The Open Reading Frame (ORF) 29-TrnC (GCA) Sequence to Detect Indica and Japonica Sub Species on Upland Rice of Situ Bagendit and Inbred Rice of Ciherang Istiana, Rohma; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12626

Abstract

The identification and the characterization of genetic diversity of rice was the first step in the rice plant breeding program. This study aimed to detect indica or japonica sub-species on upland rice Situ Bagendit and inbred rice Ciherang using molecular markers ORF 29-TrnC (GCA) on the chloroplast genome. Rice was included to the indica sub-species if the 32 bp insertion on ORF 29-TrnC (GCA) sequence was found, on the contrary, if the deletion 32 bp on ORF 29-TrnC (GCA) was found then it was included to the japonica sub-species. DNA isolation was examined from the leaves of the rice plants, and then it tested quantitatively to determine the transparency and DNA concentration from the isolation results. PCR amplification was performed using a pair of primers CP2 and it was followed by agarose gel electrophoresis. The visualization of the DNA bands used the gel documentation. Sequencing of PCR products produced a long base 390 bp in Situ Bagendit rice and 390 bp in Ciherang rice. Analysis of the sequences showed that the insertions occurred throughout the 32 bp in Situ Bagendit rice and the insertions occurred throughout the 32 bp in Ciherang rice. The results showed that upland rice Situ Bagendit and inbred rice Ciherang were included in the indica sub-species. The knowledge of variety of genetics of rice can be used as bio-information in the plant breeding program. Further, the knowledge can be used to protect in genetic power source, the selection and the composing of superior varieties of rice which is tolerant with kinds of biotic and abiotic factor.
Efek Pemberian Antioksidan Vitamin C dan E terhadap Kualitas Spermatozoa Tikus Putih Terpapar Allethrin Christijanti, Wulan; Utami, Nur Rahayu; Iswara, Arya
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1148

Abstract

Allethrin  is  one  of  synthetic  pyrethroid types which  is  used  in many mosquito repellents and has toxic potential. The aim of this research was to examine the infl uence of vitamin C and E antioxidant activity to the sperm quality of allethrin-induced albino rats. This research used a post-test randomized design. The population of the research were  8  hr/day  allethrin-induced male  albino  rats. The  sample  of  the  research was allethrin-induced male albino rats which were divided into 4 groups, i.e. group I was the control group, group II was the group treated with 1.8 mg vitamin C per day, group II was the group treated with 1.44 mg vitamin E per day, and group IV was treated with a combination of 1.8 mg vitamin C per day and 1.44 mg vitamin E per day. The duration of the treatment was 45 days. The sperm sample was taken in day 46. The data consisted of sperm count or amount, sperm motility, sperm viability, and sperm morphology, and then was analyzed with ANOVA followed by BNT. The result showed that vitamin C and E antioxidants signifi cantly infl uenced the the sperm quality of the 45-day mosquito repellent-induced rats. Overall, the allethrin-induced albino rats treated with vitamin C and E antioxidants were better in maintaining the sperm quality than single treatment of vitamin antioxidants.Keywords: allethrin, vitamin C, vitamin E, antioxidant, sperm  quality.
Ascaris suum Cuticle Ultrastructure Due to the In Vitro Application of Ethanol Extract to Fruits Musa x paradisiaca L. ‘Pisang Ambon’ Haryatmi, Dwi; Astirin, Okid Parama; Widiyani, Tetri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11918

Abstract

Species of Musa have various activities, one of which is anthelmintic activity. Part of banana plants that can be used as anthelmintic is part of the fruit, because of the phytochemical content possessed. Tannin is able to inhibit the enzyme cholinesterase, damage the membrane of the worm and can precipitate the protein.  The purpose of this research is to know the content of tannin and also total tannin level on ethanol extract of Musa x paradisiaca L. ‘Pisang Ambon’ and in vitro effect of the extract on Ascaris suum cuticle.  Thin Layer Chromatography (TLC) showed of ethanol extract of Ambon banana containing tannin with a total test of tannin was 54.98% w/w. In vitro at negative control (NaCl 0.9%) worm death occurred at 289 hours, positive control (pyrantel pamoate 5 mg/ml) at 1 hour, extract concentration 200 mg/ml at hour 27 and concentration 400 mg/ml at 1 hour. Histopathological examination and scanning electron microscope showed a degenerative change in ultrastructures of the worms cuticle. From the results of this study, it can be concluded that exposure of ethanol extract to Ambon banana give a damaging effect to cuticle ultrastructure in the form of crack/break, perforation and separation of muscular from cuticle and edema on A. suum worm. High tannin content in the raw/green ambon banana, can be developed to eradicate ascariasis including egg stage which until now cannot be done with synthetic drugs due to the thickness and strength of layers of worm eggs consisting of layers of protein and lipids.

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