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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Species Richness and Birds Conservation Priority in Mount Rinjani Areas, Lombok Hadiprayitno, Gito; Mertha, I Gde; Ilhamdi, Moh. Liwa
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5039

Abstract

Mount Rinjani area on the island of Lombok is unique in bioecology. It provides habitats for many species of birds, especially birds of Wallacea. Several studies on birds in this area have been done, but the conservation priority of these birds has not been determined. Based on this condition, the purpose of this research is to determine the priority of birds conservation in mount Rinjani areas. Exploration about the spesies of birds was started from Senaru Village to Segara Anak Lake. Abundance of each bird species was estimated using point count. Data analysis to determine the conservation priority used indicators that consist of endemicity, population status, species threatened and species management. The results showed that the birds species in mount Rinjani areas are composed of 32 species belonging to 20 families. Bird species that have high abundance were Amandava amandava (15.94%), Zosterops Montanus (15.70%), and Parus major (11.32%), while Ptilinopus melanospila and Anthus novaeseelandiae were in low abundance (0.23%). Analysis birds conservation priority in mount Rinjani areas indicates that Otus jolandae has the highest score (65) and should be the first priority for the conservation, while Philemon buceroides and Gallus varius with the same score (60) are the second priority. How to CiteHadiprayitno, G., Mertha, I. G., & Ilhamdi, M. L. (2016). Species Richness and Birds Conservation Priority in Mount Rinjani Areas. Biosaintifika: Journal of Biology & Biology Education, 8(3), 270-277.
Pemanfataan Pupuk Daun, Air Kelapa dan Bubur Pisang sebagai Komponen Medium Pertumbuhan Plantlet Anggrek Dendrobium Kelemense Hasanah, Uhwatul; R, Enni Suwarsi; -, Sumadi
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3784

Abstract

Bahan alternatif alami diperlukan untuk menggantikan bahan kimia yang mahal untuk kegiatan kultur jaringan. Penelitian ini bertujuan untuk menguji pengaruh merk, konsentrasi pupuk daun, interaksinya terhadap pertumbuhan plantlet anggrek Dendrobium dan menentukan konsentrasi yang paling optimal dalam menginduksi pertumbuhan plantlet. Percobaan dilakukan dengan rancangan acak lengkap faktorial yang terdiri dari dua perlakuan yaitu merk pupuk dan konsentrasi pupuk, masing-masing dengan tiga taraf perlakuan yaitu merk pupuk (growmore, hyponex, gandasil) dan konsentrasi (1 g/l, 2 g/l, 3 g/l). Pertumbuhan plantlet anggrek pada penelitian ini diukur berdasarkan parameter tinggi plantlet, jumlah daun, luas daun, jumlah akar dan panjang akar. Data dianalisis dengan anava dua arah, bila signifikan dilanjutkan dengan uji Duncan. Hasil anava menunjukkan merk pupuk berpengaruh signifikan terhadap penambahan jumlah daun, luas daun dan jumlah akar, konsentrasi pupuk berpengaruh signifikan terhadap penambahan tinggi plantlet dan panjang akar sedangkan interaksi merk pupuk dengan konsentrasi pupuk signifikan terhadap penambahan tinggi plantlet dan luas daun. Kombinasi perlakuan yang paling optimal yang menginduksi penambahan tinggi plantlet dan luas daun adalah pupuk hyponex dengan konsentrasi 2 g/l (5,40 cm dan 5,43 cm2). Untuk mendapatkan pertumbuhan plantlet paling tinggi dan luas daun paling optimal digunakan media pupuk hyponex dengan konsentrasi pupuk 2 g/l.Natural alternative materials needed to replace expensive chemicals for tissue culture activities. This study aimed to examine the influence of the brand, the concentration of foliar fertilizer, interaction on the growth of dendrobium orchid Dendrobium and determine the optimal concentration in inducing the growth of plantlets. Experiments were carried out with completely randomized factorial design consisting of two treatments, the brand of fertilizer and manure concentration, each with three levels, namely brand fertilizer treatments (Growmore, Hyponex, Gandasil) and concentration (1 g/l, 2 g /l, 3 g/l). Orchid plantlets growth in this study was measured by high parameter plantlets, number of leaves, leaf area, root number and root length. Data were analyzed by two-way ANAVA, if significant followed by Duncan test. Results of ANAVA showed a significant effect on the brand of fertilizer increase in the number of leaves, leaf area and number of roots, the concentration of fertilizer significantly influenced the high increase of plantlets and root length, while the interaction with the concentration of manure fertilizer brands significantly to the high increase of plantlets and leaf area. The most optimal combination of treatments that induce high addition of plantlets and leaf area was Hyponex fertilizer with a concentration of 2 g / l (5.40 cm and 5.43 cm2). To get the highest plantlet growth and optimal leaf area most used media Hyponex fertilizer with manure concentration of 2 g/l.
Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste Yanti, Nur Arfa; Ahmad, Sitti Wirdhana; Ambardini, Sri; Muhiddin, Nurhayani Haji; Sulaiman, La Ode Iman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10241

Abstract

Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS) media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel) and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production.
Effect of Red Fruit Oil on Ovarian Follicles Development in Rat Exposed to Cigarette Smoke Utami, Isrotun Ngesti; Yuniwarti, Enny Yusuf; Saraswati, Tyas Rini
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13236

Abstract

Red fruit oil (Pandanus conoideus Lam) contains active substances in the form of alpha-tocopherol, beta-carotene and unsaturated fatty acids that can potentially be antioxidants. This study aims to examine the effect of red fruit oil (Pandanus conoideus) on the development of ovarian follicles of rat exposed to cigarette smoke, (in increasing the number of primary follicles, secondary follicles, tertiary follicles and ovarian weight). This study used Completely Randomized Design with 20 female rats (3 months old) divided into 4 treatment groups: P0 (Positive control), P1 (negative control of exposure to cigarette smoke for 8 days), P2 (exposure to cigarette smoke for 8 days + 0.1 ml red fruit oil) and P3 (exposure of cigarette smoke for 8 days + 0.2 ml red fruit oil) with 5 time repetition and 28 days red fruit treatment for the research parameters were the number of primary follicles, secondary follicles, tertiary follicles and ovarian weight. The data obtained were analyzed using one-way ANOVA with 95% confidence level (P < 0.05). The results showed that the administration of red fruit oil at doses of 0.1 ml and 0.2 ml was not significantly affecting (P > 0.05) the number of primary follicles, secondary follicles, tertiary follicles and ovarian weight, so it can be conclusion that the administration of red fruit oil at a dose of 0.1 ml and 0.2 ml cannot increase the number of primary follicles, secondary follicles, tertiary follicles and ovarian weight in rat exposed to cigarette smoke.
Aktivitas Antioksidan Jus Tomat pada Pencegahan Kerusakan Jaringan Paru-Paru Mencit yang Dipapar Asap Rokok (Genetic Diversity of Banana with B Genom Using Microsatelite Marker) Marianti, Aditya
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.39

Abstract

The activities of tomato juice’s antioxidant for the prevention of lung tissues damage in mice cigarette smoke induces endogen antioxidant is no longer effective on oxidative stress control towards cigarette consumer lung, thus exogenous antioxidant is a must. A kind of exogenous antioxidant is lycopen, a content of tomato. 40 mice is divided into 5 groups with 8 of each. Group I is the controlling group, group II is the negative control, and group III, IV, V are the group of mice that is supplied by tomato juice on the 1,7 g/100 g weight/day ; 3,4 g/100 g weight/day; and 5,1 g/100 g weight/day dozes. Each group, except the control one, is forced by cigarette smoke for 15 minutes per day in 54 days. On the 55th day, all mice is sacrificed. Specimens of their lungs are made in the way of histological by haematoxylin – eosin method. The data is about the comparison of lung tissue failed on each group, that is analyzed by Anava. The observation of each tissue on the group II showed emfisema pattern, that is alveolus membrane epithelia cell with no nucleus nor endothelium, with wide alveolus lumen and loose alveolus junction. The identical situation also found on group III. The failed tissue cannot be found on group IV and V. Antioxidant of tomato is capable of controlling lung tissue failed of mice of cigarette smoke. Keywords. Tomato juice, antioxidant, cigarette smoke, lung tissues
Resistance Inheritance of Plutellaxylostella Population to Residual of Emamectin Benzoat Tarwotjo, Udi; Rahardian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8048

Abstract

Excessive use of insecticides drives the increasing ability of pests to become resistant. The objectives of this research were to study the susceptibility and the resistance inheritance of the eleven population of P. xylostella to emamectin benzoate. The leaf-dip bioassay was applied to determine the sensitivity of P. xylostella to emamectin benzoate. The offspring of backcrossed F2 were tested whether the resistance was controlled by monogenic. The results showed that the LC50 of the Selo population was 53.42 ppb, and the Puasan population was 212.13 ppb. The genetic analysis showed that the backcrosseddegree of dominance (D) was less than 1. It was indicated that the P. xylostella resistance to emamectin benzoate was recessive. The value of LC50 of the backcrossed F1? x ?S (177.99 ppb) and its reciprocals x ?R (F1) (201.69 ppb) were not significantly different with the value of LC50 resistance population. This suggests that the nature of P. xylostella resistance to emamectin benzoate was controlled by monogenic.The result of the study would be beneficial for developing strategy to maintain susceptible population using refugee plant during lack of their host.
Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains Nuryanto, Agus; Pulungsari, Anatasia Endang
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.12097

Abstract

Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identification using additional characters is vital, such as using a molecular marker. Here, we characterized Batanghari, Tambago, Orange, and Mandiangin gourami strains using the cytochrome b gene to evaluate their taxonomic status. Partial sequences of cytochrome b gene were sequenced for 40 individuals. Taxonomic status was checked for giant gourami sequences available in GenBank. Kimura 2-Parameter genetic distances were calculated in MEGA6 software. Haplotype and nucleotide diversity within population and ?st-value among populations were estimated in Arlequin software. Phylogenetic relationship was reconstructed using the neighbor-joining method in MEGA6 software based on Kimura 2-parameter model with 1000 pseudobootstraps. Taxonomic identification results in 99% sequences homology to Osphronemus goramy sequences (accession number KU984978.1 and AY763768.1), means that all strains belong to single species. Low genetic distances, medium haplotype and low-level nucleotide diversity were observed among strains. Pairwise ?st-comparison indicates no genetic differences among Sumatera strain, whereas strong genetic structures observed between Sumatera and Mandiangin strains. The phylogenetic tree showed that Mandiangin formed separate subclades from other strains with bootstraps value of 100%. This finding has important implication for breeding sciences and efforts.
A Preliminary Study of Bryophytes in Enggano Island, Bengkulu, Indonesia Nadhifah, Ainun; Surya, Muhammad Imam
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5239

Abstract

Enggano Island is one of the outer islands that belongs to the Province of Bengkulu. Furthermore, there is very limited information about the diversity of bryophyte from Sumatera, especially in lowland forest, Enggano Island. The aim of this research was to provide the initial information related to the diversity of bryophytes in Enggano. The research was conducted in six forests i.e. primary, secondary and degraded forests. The results showed that 32 number of collection from 14 families, 21 genera, and 32 species were identified. Leceuneaceae was common family for liverworts while the mosses family was dominated by Hypnaceae. None of the hornworts were found in this study. Some species identified (Taxyphyllum sp., Vesicularia sp., Riccardia sp., and Thuidium sp.) have the potential benefit and biological activity. Two genera were new records to Sumatra, Gongylanthus, and Symphyogyna. Moreover, Gongylanthus sp. and Symphyogyna sp. have important records related to the habitat.How to CiteNadhifah, A., & Surya, M. I. (2016). A Preliminary Study of Bryophytes in Enggano Island, Bengkulu, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 8(2), 201-205.
Description of Skin Anatomical Structures of Wistar Rats Exposed to X-Rays Radiation -, Lisdiana; Ernawati, Agus
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3536

Abstract

The research was aimed to find out a profile of an anatomical structure of the Rattus norvegicus skin after exposed to X-ray radiation. Research was performed by treating the 20 Rattus norvegicus at the age of 1.5 months. The weight rats were weighed approximately 100 13 g grouped into four treatments with different dose of X-ray radiation. The four treatments were 0 mgy (control), 50 mgy, 100 mgy, and 150 mgy X-ray radiation. The variable in this research was a dose of X-ray radiation and the anatomical structure of the rattus norvegicus skin. The data obtained were analyzed with qualitative description. The research results after exposure of X-ray radiation for 5-days showed that there was no damage on the skin macroanatomy. Whereas, the observation in the skin microanatomy showed that there was a damage, e.g. thinning of the epidermis, cell picnosys, cell injury, and hemoragic. The result indicated that the different dose of X-ray radiation affected the skin anatomy structure. The X-ray radiation exposure at 100 mGy on skin microanatomy were caused a thinning of the epidermis in stratum corneum layer, picnosys on the nucleus, cell injury and hemoragic.Penelitian ini bertujuan untuk mengetahui gambaran struktur anatomi kulit tikus (Rattus norvegicu) strain Wistar setelah terpapar radiasi sinar-X. Sebanyak 20 ekor tikus umur 1,5 bulan dengan berat badan sekitar 100 13 gram dikelompokkan ke dalam 4 perlakuan yaitu perlakuan dosis radiasi sinar-X sebesar 50 mGy, 100 mGy dan 150 mGy serta 1 kelompok kontrol. Paparan radiasi dilakukan selama 5 hari. Variabel penelitian ini adalah dosis paparan radiasi sinar-X dan struktur anatomi kulit. Data yang diperoleh dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa secara makroanatomi kulit tikus tidak terlihat kerusakan, tetapi secara mikroanatomi terdapat kerusakan berupa penipisan epidermis, piknosis sel, jejas sel, dan hemoragik. Hal tersebut dikarenakan besarnya dosis radiasi mempengaruhi terhadap perubahan struktur anatomi kulit. Paparan radiasi sinar-X dosis 100 mGy, menimbulkan kerusakan kulit tikus secara mikroanatomi berupa penipisan epidermis dilapisan stratum korneum, piknosis inti, jejas sel dan hemoragik.
Study of Mistletoe in Joben Resort Forest Mount Rinjani Lombok Fikriani, Wahyuni Dwi; Mulyaningsih, Tri; Aryanti, Evy
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8427

Abstract

Mistletoes are one group of hemiparasite plants, including the Lorantaceae family that have potential as medicinal. These hemiparasite plants can attack flowering plant (Magnoliophyta) and non-floweing plant (Pinophyta), especially on the main stems, branches and twigs. The objective of this research is to identify the species of mistletoe and its hosts, make identification key, descriptions, and to make a distribution map of mistletoe in Joben Resort forest south of Mount Rinjani Lombok. This study is descriptive explorative research with three kinds of collecting sample methods i.e exploration, continous strip sampling, and delenation method. The research found five species of mistletoes are included in three genera i.e Amyema cuernosensis, Amyema enneantha, Amyema tristis, Macrosolen retusus and Scurrula artropurpurea. These five kinds of mistletoe are associated with 23 hosts species of plants, 18 genera from 13 families. The most favorite host of these mistletoes is Ficus septica, and the most agresive mistletoe is Scurrula artropurpurea. The important finding of the research is finding new species or new record of mistletoes. The benefit of these new record or new species is providing new material of new medicinal for treating some diseases such as various cancers.

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