cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. Ir. Dyah Rini Indriyanti
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 20   21   22   23   24 
Chitosan as Chelating and Protective Agents from Lead Intoxication in Rat Marianti, Aditya; Anatiasara, Debi; Ashar, Fachrudyn Faisal
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8943

Abstract

Increasing accumulation of lead in body causes serious health problems. Chelation is widely used to decrease lead level in body. This research aims the effectivity of chitosan in chelating lead and their protective ability toward liver and kidney for lead acetate-induced rat. Thirty rats were divided into 6 treatment groups. All groups, except control group, were administered by 175 mgkg-1 BW lead acetate. Positive control group was treated using EDTA 50 mgkg-1 BW. Treatment group 1,2, and 3 were treated using chitosan in dose of 64, 32, and 16 mgkg-1 BW dissolved in 2% acetic acid, respectively in 30 days. The effectivity of chitosan was compared to blood lead level. ALT and AST level were measured to determine the protective ability of chitosan. Normal function of kidney was assessed using creatinine level. Results showed that blood lead level from all treatment groups, except negative control group, had no significant difference from control group. EDTA and chitosan ability in chelating lead were proven by low level of AST, ALT, and creatinine in treatment groups. This indicated that there was no significant difference from control group. Chitosan capable of chelating lead and protecting kidney and liver from heavy metal.
Keanekaragaman Jenis Vegetasi dan Profi l Habitat Burung di Hutan Mangrove Pulau Nyamuk Taman Nasional Karimunjawa Kartijono, Nugroho Edi; Rahayuningsih, Margareta; Abdullah, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1149

Abstract

Research on the vegetation diversity and bord habitat profi le has been carried outin the Mangrove Forest of Pulau Nyamuk in Karimunjawa National Park to examinethe relationship between vegetation types and mangrove habitat profi le and the spatialusage by birds. A transect method has been employed to survey the vegetation and thepoint count method was used to survey the birds. There were 14 vegetation types and 19species of birds from 14 families at two observational sites in mangrove forest in PulauNyamuk. Exchoecaria agallocha dominated the eastern part of Pulau Nyamuk, whereasLumnitzera racemosa dominated the northern part of the island. The vegetational spaceutilization by birds can be grouped based on the vertical vegetation stratifi cation, i.e. theland, the understorey, the subcanopy and the canopy.Keywords: mangrove vegetation, bird habitat, Pulau Nyamuk, Karimunjawa
Mahameru Soybean (Glycine max) Cultivar, High Salinity Tolerant Juwarno, Juwarno; Suparjana, Tata Brata; Abbas, Muachiroh
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11870

Abstract

Mahameru cultivar is high salinity tolerant cultivar. The previous study result showed Mahameru cultivar could tolerate 140mM NaCl, but Cilacap Coast salinity levels often reaching 200mM NaCl. A research of salinity stress on Mahameru cultivar at 200 mM NaCl have not conducted yet. Therefore to conduct the research of Mahameru at high salinity stress to obtained high salinity tolerant soybean cultivar.   The observed variables are anatomy (epidermis thickness, the density of stomata and trichomes, palisade thickness) physiology (the dry weight of roots and canopy, the content of chlorophyll a and b) Production (whole pod, total filled pod, total empty pod, weight per one-hundred beans). The salinity treatment was 0, 50,100, 150, 200 mM NaCl given at three days before planting and twenty-one days after planting. The data of anatomy and physiology was taken at forty-five days after planting. The production data was taken when soybean plants turned brown. The result indicates that salinity affects anatomy characteristic of leaf, higher the salinity increasing epidermis thickness and the density of stomata and trichomes. Salinity affected the content of chlorophyll a and b. Higher the salinity increased the content of chlorophyll a and b. Salinity did not affect soybean production. Based on this study Mahameru cultivar is resistant to salinity up to 200 mM NaCl. The benefit of this research help to enhance national soybean production with utilization coastal land for soybean planting Mahameru cultivar.         
Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Kurniati, Tri Handayani; Rusmana, Iman; Suryani, Ani; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity. Analysis of microbial growth parameters showed that AMP10 grew best at 50gmL-1pyrene concentration, leading to 96% degradation of pyrene within 7days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsificationindex (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H., Rusmana, I. Suryani, A. & Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology & Biology Education, 8(3), 336-343.
Optimasi Jenis dan Konsentrasi ZPT dalam Induksi Kalus Embriogenik dan Regenerasi menjadi Planlet pada Carica pubescens (Lenne & K.Koch) Sari, Nika; R, Enni Suwarsi; -, Sumadi
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.3785

Abstract

Carica pubescens (Lenne & K. Koch) Badillo atau Vasconcellea pubescens, di Indonesia hanya ditemukan di daerah pegunungan Dieng dan Bali. Kultur jaringan merupakan teknik perbanyakan yang efisien. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh jenis dan konsentrasi ZPT terhadap induksi kalus embriogenik pada media MS pada C. Pubescens. Penelitian pertama (induksi) merupakan percobaan 2 faktor yang disusun menggunakan rancangan acak petak tersarang (Split block Design) dengan 4 ulangan. Penelitian kedua (regenerasi kalus) merupakan percobaan satu arah yang disusun dengan menggunakan rancangan acak lengkap (RAL) dengan 4 ulangan. Analisis menggunakan ANAVA dan uji lanjut BNT dengan taraf kesalahan 5%. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik dari eksplan jaringan daun paling tinggi didapatkan pada perlakuan ZPT 2,4-D 3 mg/l + TDZ 1 mg/l yang ditambahkan pada media MS, menghasilkan persentase kalus dan berat kalus yang paling tinggi. Untuk regenerasi jenis ZPT BAP dengan konsentrasi 4 mg/l dalam media MS arang aktif yang mengandung NAA 0,2 mg/l, menghasilkan persentase pembentukan kalus menjadi tunas dan jumlah kalus yang membentuk tunas dengan hasil yang optimal. Berdasarkan hasil penelitian disarankan untuk menginduksi kalus embriogenik menggunakan kombinasi ZPT 2,4-D dan thidiazuron, sedangkan untuk regenerasinya menggunakan ZPT BAP dan NAA.In Indonesia, Carica pubescens (Lenne & K. Koch) Badillo and Vasconcellea pubescens only found in mountainous areas in Dieng and Bali. Tissue culture is an efficient propagation technique. The aim of this research was to assess the effect of the type and concentration of plant growth regulator on embryogenic callus induction on MS medium in C. Pubescens. The first study (induction) was a two factor experiment were prepared using a nested plot randomized complete block design (Split block design) with 4 replications. The second study (callus regeneration) is a one-way experiment which prepared using a completely randomized design (CRD) with four replications. Data were analyzed using ANAVA and LSD to further test error level of 5%. The results showed that the induction of embryogenic callus from leaf tissue explants obtained at the highest 2,4-D treatment PGR 3 mg/l+TDZ 1 mg/l were added to the media MS, produce weight percentage of callus and callus highest. For regeneration of PGR BAP with a concentration of 4 mg/l in MS medium containing activated charcoal NAA 0.2 mg/l, a greater percentage of callus formation into buds and the number of callus forming buds with optimal results. Based on the research results, it suggested to induce embryogenic callus using PGR combination with 2,4-D and thidiazuron, while for regeneration was suggested to use PGR BAP and NAA.
Screening Endophytes of Neem Leaf that Potential Anti-Anthrax through Tests of Anti Staphylococcus Aureus Ananda, Khuzyia Rizqi Triavi; Sunarno, Sunarno; Zulfikar, Muhamad Fikri; Avisha, Hafsah; Nastain, Muhamad; Abdullah, Ridwan
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13956

Abstract

Anthrax is a deadly disease caused by Bacillus antracis that damages the lymphatic and vascular systems of animals and humans. Anthrax prevention in Indonesia has been done but has many shortcomings among them is a lot of dead animal raises after vaccination and relatively expensive. The azadirachtin bioactive compound produced by neem endophytic microbes is known to inhibit the growth of B. anthracis bacteria, thus potentially as an anti-anthrax agent. Other bacteria that have the same pathogens as B. antrachis are Methycillin Resistant Staphylococcus aureus (MRSA) so that it is used as a research object. The aim of this research is to obtain the active biomaterials from selected neem leaf endophytes to be tested in S. aureus MRSA as bacteria with Anthrax pathogens. The method used in this research is the isolation, selection, and extraction of Neem  endophytes. The process of isolation is done by growing on the general media and the selection process on special media. The bioactive extraction process uses 1000 rpm centrifuge and screening on MRSA culture medium. Inhibitory zone tests were used to determine the effect of endophytic biopsy in inhibiting MRSA growth. The result of the research is that the bioactive endophytes of neem are able to inhibit MRSA growth which is characterized by the formation of drag zone around the endophytic bioactive. Thus it can be concluded that the use of neem endophytes can be a new solution to eradicate the presence of anthrax disease in the livestock and people in Indonesia with safe research methods, relatively inexpensive and ineffective vaccine replacements.
Variasi Panjang Fragmen Gen ND3 Burung Famili Ploceidae Endemik Pulau Jawa (The Length Variation of ND3Gen Fragmen of Java’s Endemic Ploceidae Family’s Birds) Fitria, Ana; Susanti, R
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.40

Abstract

Ploceidae family’s birds in Indonesia consist of 41 species include 13 species as Java’s endemic. Some species of Ploceidae family were start to rarely oberved, because of new house developing and hunting as a pets, zoo collection and education kit. It’s need some efforts to conserve these rare species through improving the habitat, rehabilitation, nursery, controlling, law inforcement for hunters, and genetic conservation according to a knowledgement of genetic variation. The research aimed to knew the length fragmen of ND3 DNAmt Gen and genetic variation among species of Ploceidae family’s birds. 11 species were observe for morphology’s characteristic than blood sample were collected for DNA isolation with Dixit methode. ND3 gen were amplificated by DNA isolated with PCR used H11151 and L10755 primer. PCR’s gain were visualized with 2 % agarose gel. The length fragmen of ND3 DNAmt were 321bp for Bondol Jawa, 338 bp for Bondol Haji and Bondol Peking, 393 bp for Burung Gereja Erasia, 413 bp for Manyar Emas, 406 bp for Gelatik Jawa, 334 bp for Manyar Tempua and Manyar Jambul, 351 bp for Pipit Benggala, 333 bp for Bondol Hijau Binglis, 317 bp for Pipit Zebra. The conclusion of this research were : 1). the length variation of ND3 DNAmt Gen among 11 species range from 317 – 413 bp and 2). morphological variation and length variation of ND3 DNAmt Gen shows that there was genetic variation among species of Ploceidae family’s birds. Key words : Ploceidae, ND3 Gen, Java’s endemic
Bioinformatics Education in Greece: A Survey Paraskevopoulou-Kollia, Efrosyni-Alkisti; Bagos, Pantelis G
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7257

Abstract

Bioinformatics is an interdisciplinary field, placed at the interface of Biology, Mathematics and Computer Science. In this work, we tried for the first time to investigate the current situation of Bioinformatics education in Greece. We searched the online resources of all relevant University Departments for Bioinformatics or relevant courses. We found that all the Departments of Biological Sciences include in their curricula courses dedicated to Bioinformatics, but this is not the case for Departments of Computer Science, Computer Engineering, or Medical Schools. Despite the fact that large Universities played a crucial role in establishing Bioinformatics research and education in Greece, we observe that Universities of the periphery invest in the field, by including more relevant courses in the curricula and appointing faculty members trained in the field. In order for us to triangulate we didnt confine ourselves to online resources and descriptive statistics but we also included interviews so as to have a more spherical view of the subject under discussion. The interviews provided useful insights regarding the teaching methods used by bioinformatics tutors, their attitudes and the difficulties they encounter. The tutors mentioned also the material that they choose, the audiences attraction techniques and the feedback they receive.
Morphology and Anatomy Characteristic of Pisang Awak (Musa paradisiaca cv. Awak) in West Kalimantan Sunandar, Ari; Kahar, Adi Pasah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11258

Abstract

Indonesia is the origin and center of diversity of banana. One of an edible banana in Indonesia is Pisang Awak (Musa paradisiaca cv. Awak ). In West Kalimantan, the ripe Pisang Awak has been processed into sale (dried banana). The aims of this research were to describe the morphological and anatomical character of Pisang Awak in West Kalimantan, Indonesia. In this study, Pisang Awak were collected from Padang Tikar I village, Batu Ampar Sub-district, Kubu Raya district, West Kalimantan. Morphological characterizations were conducted by following the instruction on Descriptors for Banana (Musa spp.) from IPGRI. The root, leaf blade, and petiole were fixed in FAA solution. Root, leaf, and petiole anatomy preparats were made by paraffin method. The lamina of Pisang Awak consisted of adaxial epidermis, two hypodermis layers, two palisade layers, spongy layer, bundle sheath cell, abaxial epidermis, laticifer. The petiole of Pisang Awak composed of three tissue systems, i.e., epidermis layer, parenchyma tissue and vascular tissue. The root of Pisang Awak consists of two epidermis layers, parenchyma and vascular cylinder. In the future, morphological and anatomical character in Pisang Awak could be applied as the basis of information for breeding programs of banana cultivars and classification.
The Correlation Between Dietary Habits and Dental Hygiene Practice with Dental Caries Among School Children at Urban Area in Semarang Ali, Omaran Ibrahim Mohammed; Oedijani, Oedijani; Muis, Fatimah
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6489

Abstract

Dental caries is a major cause of tooth loss in children and young adults. Dental caries have been linked to the situation of underprivileged families, nutritional imbalance, and poor oral hygiene techniques, including lack of tooth brushing or flossing the teeth, and also have a genetic etiology. Dietary habits and dental hygiene practice can result in high caries in school children. This research aimed to reveal the correlation between dietary habits and dental hygiene practice with dental caries among school children in urban area of Semarang. The subjects of this research are the elementary student 7 9 years old enrolled in schools located in at urban area in Semarang in 2016 and the mother of a student who became the study sample. Data were statisically analyzed usingbivariate analysis and multivariate analysis. Based on the research result, it can be concluded that: there was no correlation between total carbohydrate intake, refined carbohydrate intake, fiber intake, dental hygiene practice with dental caries, bottle feeding and duration of bottle feeding were assosiated with dental cariest-score. Overall, def-t score in the study was very bad with high median of dental caries score and many children have dental caries t-score more than 6.How to CiteAli, O. I. M., Muis, F. & O, Oedijani. (2016). The Correlation Between Dietary Habits and Dental Hygiene Practice with Dental Caries Among School Children at Urban Area in Semarang. Biosaintifika: Journal of Biology & Biology Education, 8(2), 178-184.

Page 22 of 107 | Total Record : 1068

 20   21   22   23   24 

Filter by Year

2009 2023


Reset
Filter By Issues
All Issue Vol 15, No 3 (2023): December 2023 Vol 15, No 2 (2023): August 2023 Vol 15, No 1 (2023): April 2023 Vol 14, No 3 (2022): Desember 2022 Vol 14, No 2 (2022): August 2022 Vol 14, No 1 (2022): April 2022 Vol 13, No 3 (2021): December 2021 Vol 13, No 2 (2021): August 2021 Vol 13, No 1 (2021): April 2021 Vol 12, No 3 (2020): December 2020 Vol 12, No 2 (2020): August 2020 Vol 12, No 1 (2020): April 2020 Article-in-Press Vol 12, No 1 (2020): April 2020 Vol 11, No 3 (2019): December 2019 Vol 11, No 2 (2019): August 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 3 (2019): Article-in-Press Vol 10, No 3 (2018): December 2018 Vol 10, No 3 (2018): December 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 1 (2018): April 2018 Vol 10, No 1 (2018): April 2018 Vol 9, No 3 (2017): December 2017 Vol 9, No 3 (2017): December 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 1 (2017): April 2017 Vol 9, No 1 (2017): April 2017 Vol 8, No 3 (2016): December 2016 Vol 8, No 3 (2016): December 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 1 (2016): March 2016 Vol 8, No 1 (2016): March 2016 Vol 7, No 2 (2015): September 2015 Vol 7, No 2 (2015): September 2015 Vol 7, No 1 (2015): March 2015 Vol 7, No 1 (2015): March 2015 Vol 6, No 2 (2014): September 2014 Vol 6, No 2 (2014): September 2014 Vol 6, No 1 (2014): March 2014 Vol 6, No 1 (2014): March 2014 Vol 5, No 2 (2013): September 2013 Vol 5, No 2 (2013): September 2013 Vol 5, No 1 (2013): March 2013 Vol 5, No 1 (2013): March 2013 Vol 4, No 2 (2012): September 2012 Vol 4, No 2 (2012): September 2012 Vol 4, No 1 (2012): March 2012 Vol 4, No 1 (2012): March 2012 Vol 2, No 2 (2010): September 2010 Vol 2, No 2 (2010): September 2010 Vol 2, No 1 (2010): March 2010 Vol 2, No 1 (2010): March 2010 Vol 1, No 1 (2009): March 2009 Vol 1, No 1 (2009): March 2009 More Issue