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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Development of Life Skill-Based Learning Instruments: Biotechnology Material in Junior High School Parnonansia, Sekar Jatiningrum; Mustikaningtyas, Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3537

Abstract

The result of preliminary observations in Empu Tantular Semarang junior high school showed that the learning instruments was still focused on the achievement of cognitive competence. The present study aimed to develop a life skill-based learning instruments of biotechnologi material. Learning instruments including syllabi, lesson plans, worksheets, scale attitudes sheets, and vocational skills assessment sheet, as well as to test its feasibility and effectiveness. This study was Research and Development, whereas the trial product design was a One Shot Case Study pattern. Trials of limited scale and large scale were done in the student of class IX at odd semester of year 2014/2015. The results of the three expert assessment of learning instruments showed very feasible criteria to be used. Inter-rater correlation analyzes were performed using the SPSS16 program which obtained a yield of 0.99. It indicated that the inter-rater reliability was exceptionally high. Classical completeness to the cognitive learning, affective and vocational skills of students of class IX A were 92.1%; 97.4%; 92.1%, respectively. Meanwhile, the results of class IX D were 74.4%; 94.9%; 100%, respectively. The percentage of student who reach the level of adherence to the learning ?61% were 100% in class IX A and 87.2% in class IX D. Therefore, the developed learning instrument was fit to be used and also effectively applied in the Empu Tantular Semarang junior high school. Beside of improving student cognitive competence in Empu Tantular Semarang junior high school, development of life skill-based learning instruments can also provide knowledge and skills that can be used so that students can make a positive contribution in later life as a member of the family and society.Hasil Observasi awal di SMP Empu Tantular Semarang, menunjukkan perangkat pembelajaran yang diterapkan masih terfokus pada pencapaian kompetensi kognitif. Penelitian ini bertujuan untuk mengembangkan perangkat pembelajaran IPA berbasis kecakapan hidup materi bioteknologi yang meliputi silabus, RPP, LKS, dan instrumen penilaian serta menguji kelayakan dan efektivitasnya. Metode penelitian yang digunakan adalah Research and Development (R&D). Desain uji coba produk menggunakan pola One Shot Case Study. Uji coba skala terbatas dan skala luas dilakukan pada peserta didik kelas IX semester gasal tahun ajaran 2014/2015. Hasil penilaian dari ketiga pakar terhadap silabus, RPP, LKS, lembar skala sikap, dan lembar penilaian kecakapan vokasional mencapai kriteria Very Feasible digunakan. Analisis korelasi inter rater yang dilakukan menggunakan program SPSS16 memperoleh hasil sebesar 0,99. Hal ini menunjukkan bahwa reliabilitas inter rater/antar penilai tergolong sangat tinggi. Ketuntasan klasikal untuk hasil belajar kognitif, afektif dan kecakapan vokasional dari kelas IX A berturut turut 92,1%; 97,4%; 92,1% dan kelas IX D berturut turut 74,4%; 94,9%; 100%. Persentase jumlah anak yang mencapai tingkat keterlaksanaan pembelajaran ? 61% dari kelas IX A sebesar 100% dan kelas IX D sebesar 87,2%.Perangkat pembelajaran yang dikembangkan Very Feasible digunakan dan efektif diterapkan di SMP Empu Tantular Semarang.
Improving Post-graduate Students Learning Activities through Lesson Study in Learning Forest-Prototype Muhfahroyin, Muhfahroyin; Oka, Anak Agung
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.6208

Abstract

Improving learning quality in 21st can not be separated from contextual learning and student-centered learning paradigm. The contextual lesson study program conducted in learning-forest prototype to build a learning community. The objectives of this research were to improve learning activities of postgraduate students in Biology Education department and to build a learning community. The implementation of lesson study was conducted in the Biology Learning Innovation subject for postgraduate students while practicing to observe open lesson in the undergraduate students which used learning forest-prototype. The postgraduate students took roles as planner, observer, and reflector in the plan, do (open lesson), and see (reflection) activities. The implementation was done in three cycles in even semester of academic year 2015/2016. Students learned collaboratively and contextually. The postgraduate students learning activities were observed by six observers from lecturer colleagues. The research results showed that the students were able to implement planning, open lesson, and reflection properly. The average of students learning activity grade was 91.11% from all of students, with the grade averages for planning, open lesson, and reflection activities were 88.89%, 93.33%, and 91.11% respectively. The implementation of this lesson study in the learning forest-prototype can be done in other relevant subjects to strengthen learning activities.
Effectiveness of Problem-Based Learning Module as An Instructional Tool in Improving Scientific Argumentation Skill Nurinda, Sindy; Sajidan, Sajidan; Prayitno, Baskoro Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.12600

Abstract

Scientific argumentation skill required to be mastered in this era. Unfortunately many students still have strunggle improving their scientific argumentation skills. This study was aimed at describing the effectiveness of ecosystem module grounded in Problem-Based Learning in improving scientific argumentation skills. This research was part of the field testing of R&D, a research and development previously conducted. Research participants included tenth grade senior high school students. Two classes were selected as a control and experiment class. The control class (existing) did not use the module but the experiment class use the module (module class) grounded in Problem Based Learning implemented. Data were collected from written tests and observations. Data analysis used the scoring rubric of scientific argumentation skill aspect which their results were tested with Gain test and independent T-test. The findings showed an increase in scores of the four aspects of scientific argumentation skills from the module class. The highest percentage of aspect was rebuttal aspect (27.27%) followed by evidence 26,06% and reasoning 23.94%, while the aspect of claim increased by 20.61%. The independent t-test results showed that there was a significant difference between pre-test and post-test results of the module class. It can be concluded that the ecosystem module with problem-based learning material was effective to improve students’ scientific argumentation skills. The product of this reasearch can be use as a tools to improve students’ scientific argumentation skills.
Proteases from Latex of Euphorbia spp. and Its Application on Milk Clot Formation Fibriana, Fidia; Upaichit, Apichat
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3951

Abstract

Crude proteases were extracted from Euphorbiaceae family, i.e. E. milii var imperata, E. trigona, and E. maculata. Among those three crude proteases, the activity of protease from E. trigona was the highest (812.50 U/ml), whereas E. milii and E. maculata crude proteases activity were 298.60 U/ml and 95.80 U/ml, respectively. E. maculata protein concentration was the highest among those three crude enzymes (1.206 mg/ml). The optimum pH and temperature of the enzymes were pH 7.0, pH 6.0, pH 6.5 and 60 C, 50 C, and 50 C, respectively. Crude protease from E. milii var imperata, E. trigona, and E. maculata retained proteolytic activity over a wide range of pH (5.09.0) and temperature (up to 65 C) with casein as substrate. All crude proteases showed milk clotting activity ranged from 0.58 U/ml to 1.01 U/ml. Thus, these crude proteases are potential to be applied in dairy industries. However, further study on enzyme purification and characterization are necessary to obtain high purity of proteases before its application.Protease kasar berhasil diekstrak dari tanaman family Euphorbiaceae, yaitu E. milii var imperata, E. trigona, dan E. maculata. Diantara ketiga protease tersebut, aktivitas protease tertinggi diperoleh dari E. trigona (812,50 U/ml), sedangkan aktivitas protease dari E. milii dan E. maculata adalah 298,60 U/ml dan 95,80 U/ml, berturut-turut. Konsentrasi total protein tertinggi terdapat pada protease kasar E. maculata (1,206 mg/ml). pH dan suhu optimum ketiga enzim tersebut adalah pH 7.0, pH 6.0, pH 6.5 dan suhu 60 C, 50 C, and 50 C, berturut-turut. Protease kasar dari E. milii var imperata, E. trigona, dan E. maculata menunjukkan aktivitas proteolitik pada rentang pH 5.09.0 dan rentang suhu sampai 65 C menggunakan kasein sebagai substrat. Semua protease kasar menunjukkan aktivitas penggumpalan susu dengan rentang dari 0,58 U/ml sampai 1,01 U/ml. Berdasarkan hasil yang diperoleh, protease kasar dari ketiga jenis tanaman ini berpotensi untuk diaplikasikan dalam industri olahan susu. Meskipun demikian, studi lanjut mengenai purifikasi dan karakterisasi sangat diperlukan untuk memperoleh protease murni sebelum aplikasi dalam industri makanan, khususnya pada industri olahan susu.
Rambut Sebagai Bioindikator Pencemaran Timbal Pada Penduduk di Kecamatan Semarang Utara Marianti, Aditya; Prasetya, Agung Tri
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.2568

Abstract

Timbal (Pb) bersifat toksik, karsinogenik, bioakumulator dan biomagnifikasi. Bioakumulasi Pb dapat terjadi pada kuku, hati dan rambut. Penelitian bertujuan untuk mendeteksi apakah telah terjadi pencemaran timbal pada penduduk di kecamatan Semarang Utara dengan menggunakan rambut sebagai bioindikator. Penelitian deskriptif eksploratif ini menggunakan populasi seluruh penduduk di kecamatan Semarang Utara. Sampel diambil dengan teknik purposive sampling yaitu penduduk laki-laki berumur antara 17 sampai dengan 50 tahun yang tinggal di kelurahan Tanjung Mas dan Bandarharjo Semarang Utara, minimal selama 5 tahun terakhir terus menerus. Kadar timbal pada tubuh dideteksi dari kadar timbal yang terakumulasi pada rambut. Hasil penelitian menunjukkan terdapat kandungan timbal pada rambut 56 sampel. Kadar timbal tertinggi sebesar 17,028 ppm dan rata-rata 8,304 ppm. Penyebabnya diduga bukan berasal dari emisis kendaraan bermotor di jalan raya mengingat profesi sampel sebagian besar adalah nelayan, tetapi kemungkinan berasal dari air minum yang mereka konsumsi. Sampel air minum yang dikonsumsi terdeteksi mengandung timbal rata-rata 6 ppm. Simpulan dari penelitian ini telah terjadi pencemaran timbal pada sebagian penduduk Semarang Utara dengan tingkat ringan sampai sedang. Pencemaran diduga berasal dari air minum yang dikonsumsi.Lead (Pb) are toxic, carcinogenic, bioaccumulator and biomagnification. Pb bioaccumulation may occur in the nail, liver and hair. The research aimed to detect whether lead pollution has affected the people in North Semarang, using hair as bioindicator. The population of this explorative descriptive research was all residents of Sub-District of North Semarang. Samples were taken using purposive sampling technique, i.e. male residents of Tanjung Mas and Bandarharjo aged between 17 and 50 years, who have stayed in the sites for at least 5 years without interruption. The lead level in their body was detected from their hair strand using AAS (Atomic Absorption Spectrophotometery). Research showed that lead substance was detected in hair strands from 56 people. The highest level was 17.028 ppm and the average level was 8,304 ppm. It was suggested that the lead pollutant was not from motor vehicles emisions on the roads cause most samples were fishermen, instead it was suspected that the drinking water was the source of the lead. It was supported by the fact that the drinking water consumed daily by the residents has been contaminated by lead at the level of 6 ppm. Conclusion the lead pollution has affected some residents of North Semarang, ranging from low level to medium level. Presumably, the pollution was originated from the consumed drinking water.
Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity Wijanarka, Wijanarka; Kusdiyantini, Endang; Parman, Sarjana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7263

Abstract

On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate () 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.
Improving Silvofishery Management Through Seedling Growth Environment Quality Dynamic Relation Analysis Hastuti, Endah Dwi; Hastuti, Rini Budi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10490

Abstract

Mangrove plants are sensitive to environment condition. This research aimed to analyze the linkages of mangrove growth and environment dynamics and to estimate the growth of mangrove along with the environment dynamics. The research was conducted through the field experiment by the plantation of A. marina in silvofishery pond canals. Data collection was conducted for 18 months with 3 months observation interval. The environment variables observed including temperature, salinity, turbidity, pH, dissolved oxygen, TSS, sediment organic matter, nitrogen and phosphorus, and the growth of mangrove seedling. Analysis was conducted through regression and modelling with Powersim software. The result showed that the height growth was affected by dissolved oxygen, temperature, salinity, turbidity and pH, while the diameter growth was affected by TSS concentration. Inversely, the growth of mangrove also had a significant effect on temperature, change of organic matter and nutrient sediment concentration. Simulation showed that the height and diameter growth rates of A. marina seedling were dynamically changed among periods. Simulated seedling growth for one year forecast resulted in the rate of 0.115024 to 0.282294 cm/day for height and 0.001287 to 0.006031 cm/day for diameter. The simulation also indicated the continuous accumulation of organic matter and nitrogen over time. This research concluded that under limited environment dynamic, the growth of mangrove could be estimated. This model is a novelty in ecological studies. This research might initiates the more advanced ecological studies. Systematic estimation of ecosystem behaviour could be applied to formulate the best management practices, particularly in the silvofishery activities.
Growth Pattern and Copper Accumulation in Callus of Datura metel Nurchayati, Yulita; Santosa, Santosa; Nugroho, Laurentius Hartanto; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5177

Abstract

This experiment was aimed to evaluate the copper accumulation using callus culture of Datura metel L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl2.2H2O and Na2CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 M. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20th doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl2.2H2O at 5 15 M of level concentration but declined at 20M. The maximum rate of accumulation of Cu (0,1519 mg g-1) was obtained at 15 M. Instead, the addition of Na2CuEDTA at 5 20M of level concentration showed the significant increment while the maximum accumulation was obtained at 20M (0,1420 mg g-1). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of D. metel as that fairly absorbed and accumulated Cu2+. Exposure with CuCl2.2H2O indicated higher accumulation than Na2CuEDTA.How to CiteNurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. Biosaintifika: Journal of Biology & Biology Education, 8(2), 135-140.
Analisis Sebagian Sekuen Gen Ferritin2 pada Padi (Oryza sativa L.) Indragiri Hilir, Riau Nugraha, Fadel; Indriyani Roslim, Dewi; Putri Ardilla, Yolla; -, Herman
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3102

Abstract

Ion Fe bebas sangat beracun bagi tanaman, karena dapat membentuk radikal bebas di dalam sel. Walaupun demikian, tanaman memiliki mekanisme untuk mempertahankan homeostasis Fe di dalam sel yang melibatkan protein ferritin. Penelitian ini bertujuan untuk menganalisis dan membandingkan sekuen nukleotida gen ferritin2 dari dua genotipe padi lokal (Bakung dan Serei) dari Indragiri Hilir, Riau dengan varietas padi rawa unggul tahan cekaman kelebihan Fe (Siam Sintanur), padi unggul tidak tahan cekaman kelebihan Fe (IR64), dan data sekuen nukleotida kultivar Nipponbare. Metode penelitian meliputi isolasi DNA toal dari daun segar tanaman padi menggunakan metode CTAB dan amplifikasi DNA (PCR) menggunakan primer OsFer_F3 (forward) dan Gross_R (reverse). Produk PCR kemudian disekuensing dan disejajarkan. Pada penelitian ini telah diperoleh fragmen dari gen ferritin2 dari kelima genotype atau varietas padi yang diuji, yang berukuran sekitar 1200 pb. Analisis pensejajaran menunjukkan terdapat 56 SNP (single nucleotide polymorphism) pada sekuen nukleotida gen ferritin2 tersebut. Bakung menunjukkan kedekatan yang tinggi dengan Nipponbare, diikuti dengan IR64, Siam Sintanur, dan Serei. Kemungkinan Bakung merupakan genotipe padi lokal dari Indragiri Hilir, Riau yang tahan cekaman kelebihan Fe.Free Fe ions are highly toxic to plants, because it can form free radicals in the cells. However, plants have mechanisms to maintain Fe homeostasis in the cells involving ferritin proteins. This study was aimed to analyze and to compare the nucleotide sequence of ferritin2 gene in two local rice genotypes (namely Bakung and Serei) from Indragiri Hilir, Riau and in Fe overload-tolerant rice variety (Siam Sintanur), Fe overload-sensitive rice variety (IR64), as well as the nucleotide sequence of Nipponbare rice cultivar. The research methods consisted of DNA isolation from fresh leaves of rice plants using CTAB method and DNA amplification (PCR) using a couple of primers, OsFer_F3 (forward) and Gross_R (reverse). The PCR products were then sequenced and aligned. DNA fragments of ferritin2 gene with length of approx. 1200 bp were obtained from those four rice varieties or genotypes tested. Alignment revealed 56 SNPs (single nucleotide polymorphisms) in the ferritin2 gene sequences. Bakung showed close distance with Nipponbare, followed by IR64, Siam Sintanur, and Serei. It wa suggested that Bakung was Fe overload-tolerant local rice genotype from Indragiri Hilir, Riau.
The Potential of Flora and Fauna as Tourist Attractions in Biodiversity Park of Pelawan Forest, Central Bangka Henri, Henri; Hakim, Luchman; Batoro, Jati
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9225

Abstract

Indonesia has a high potential for the diversity of flora and fauna species together with their ecosystem. Preservation of natural resources can be done through conservation using the concept of ecotourism. The purpose of this research is to identify the potential of the flora and fauna in Biodiversity Park of Pelawan Forest for tourist attraction. The study was conducted from October 2016 to January 2017 through inventory and in-depth interview. The analysis on the results of this study indicate that there are 41 species of 27 families of plants and 135 species of animals consisting of amphibians (6 species), reptiles (16 species), birds (99 species) and mammals (14 species). This indicates that Biodiversity Park of Pelawan Forest is very competitive for tourism attractions, which is supported by the presence of key species (Tristaniopsis merguensis), flagship species (Cephalopachus bancanus), and abundance of birds for bird watching. Based on the IUCN red list, several species of flora, such as Gonystylus bancanus, and fauna, such as Setornis criniger, Chloropsis sonnerati, Macaca nemestrina, Nycticebus menagensis, and Cephalopachus bancanus, are vulnerable to extinction. This study on flora and fauna results in the initial data that can be used to support conservation efforts. Moreover, the result of this study can provide an opportunity for visitors to enjoy these tourist attractions, which can benefit the local community.

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