Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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<![CDATA[ANTHRACNOSE DISEASE ON VEGETABLES CROPS IN SERANG VILLAGE, DISTRICT OF KARANGREJA, PURBALINGGA REGENCY ]]>
<![CDATA[Sucianto, Eddy Tri]]>;
<![CDATA[Abbas, Muachiroh]]>;
<![CDATA[Purwati, Endang Sri]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.21818
<![CDATA[Various vegetables are widely cultivated in Serang Village, District of Karangreja, Purbalingga Regency. This village is located at 1350 above sea level. Vegetable productions in Serang are often constrained by the presence of diseases caused by pathogenic fungi Colletotrichum sp. called anthracnose. However, there was no data regarding anthracnose attack in vegetable crops in Serang. This study aimed to find out the types of vegetable that were attacked by anthracnose disease as well as the intensity of the attack in a vegetable crops in Serang Village, Karangreja District, Purbalingga Regency. Vegetables with anthracnose were purposively selected from 10 different locations. The anthracnose disease was identified based on their symptoms and analyzed descriptively. The results showed that five different vegetable crops suffer from anthracnose, and the intensity of anthracnose ranges from 32% up to 52%. Those intensity percentages indicate that large part of vegetable crops in Serang Village was attacked by anthracnose and possibly cause a significant decrease in vegetable production in that village. Our results provide the first scientific data about anthracnose attack in Serang Village. The data is essential for the management of vegetable crops in Serang Village.]]>
<![CDATA[MOLECULAR BARCODING REVEAL THE EXISTENCE OF MOLE CRABS EMERITA EMERITUS IN NORTH COAST OF CENTRAL JAVA ]]>
<![CDATA[Bhagawati, Dian]]>;
<![CDATA[Winarni, Elly Tuti]]>;
<![CDATA[Nuryanto, Agus]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.20497
<![CDATA[The existence of mole crabs had been reported from the southern coast of Central Java. However, no mole crab data was available from the northern coast Central Java. This study aimed to figure out the existence of mole crabs in the northern coast of Central Java as revealed from the cytochrome c oxidase 1 (COI) barcoding. Mole crabs samples collected in Sendang Sikucing Beach Kendal. Taxonomic status of the samples was inferred from sequences similarity test using basic local alignment search tool to conspecific sequence deposited in GenBank. Pairwise genetic distances were calculated based on the Kimura 2-parameter model. A phylogenetic tree had reconstructed in molecular evolution genetic analysis (MEGA) software based on neighbor-joining algorithm. Branching reliability was obtained from 1000 bootstraps replication while branching polarity was obtained from the out-group comparison. The mole crab samples from the northern coast of Central Java had unambiguously identified as Emerita emeritus based on high sequences similarities (98.27%-99.70%), low genetic distances (0.002-0.005), and their monophyly with Emerita emeritus (KR047035) in GenBank. Pairwise analysis among each possibility pair of samples had genetic distances ranged between 0.000 and 0.005, indicated that all sample belong to single species. The data provide the first record of the existence of Emerita emeritus in the northern coast of Central Java. Our data on the existence of E. emeritus in the northern coast of Central Java is among essentials information as a scientific basis in creating policy for the management of sustainable use of the mole crabs in the areas.]]>
<![CDATA[DETECTION OF POWDERY MILDEW RESISTANCE GENE IN MELON CULTIVAR MELONI BASED ON SCAR MARKERS ]]>
<![CDATA[Ishak, Muhammad Alif]]>;
<![CDATA[Daryono, Budi Setiadi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.22198
<![CDATA[Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.]]>
<![CDATA[POTENTIALS OF CARBON STORED IN PLANT BIOMASS AT LITTLE FARMERS GRASSLAND CISARUA, WEST BANDUNG REGENCY ]]>
<![CDATA[Malik, Annas Dwitri]]>;
<![CDATA[Zso Zsa Dewa, Komang Yoga]]>;
<![CDATA[Parikesit, Parikesit]]>;
<![CDATA[Withaningsih, Susanti]]>;
<![CDATA[Wingit, Ratna]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23111
<![CDATA[Alternatives of vegetations to store carbon need to be encouraged considering that forests are threatened by widespread destructions. One such vegetation is grasslands which have the potential for carbon storage and to reduce CO2 concentration in the atmosphere. At present, many enterprises have designed grasslands for animal feed. Grassland at Little Farmers, Cisarua, West Bandung was established for many purposes, i.e. recreation, education, and animal feed. The purpose of this research was to study the potential of carbon stock in grassland vegetation at this location. Based on RaCSA method, the tree biomass was determined by nondestructive collection of density and basal area of trees, then calculated by an allometric equation. The ground cover biomass was determined by destructive collection of grass and roots. Total measured biomass was multiplied by 46% to obtain carbon storage. Based on the results, the potential of carbon stock in Little Farmers grassland is 6,506.23 kg/m2 with the potential for carbon storage below the ground (0.129 kg / m2) was slightly higher than the carbon stored above the ground (0.101 kg / m2). Carbon stored in ground cover had been proven to be lower than woody plants (6,506 kg / m2). This study has pioneered in finding the carbon stocks potential of a man-made grassland, so it provides basis of an alternative land use that can be encouraged for carbon sequestration. For many enterprises, this study will aid in the conduct and management planning of grasslands with regards to ecosystem services preservation, such as carbon sequestration.]]>
<![CDATA[THE POTENTIAL OF CNIDOSCOLUS CHAYAMANSA ALCHOHOLIC LEAVES EXTRACT AS HYPOLIPIDEMIA AGENT ]]>
<![CDATA[Iswari, Retno Sri]]>;
<![CDATA[Mubarok, Ibnul]]>;
<![CDATA[Sasi, Fitri Arum]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23687
<![CDATA[Cardiovascular disease caused by hyperlipidemia is the highest case in term of fatality of non-infectious disease in Indonesia. Cnidoscolus chayamansa is well known as a herb that has nutraceutical potential for medicine and likely as a hypolipidemia agent. Therefore, this study aimed to identify the optimal dose of C. chayamansa as a candidate to treat cardiovascular diseases. A total of 30 white rats were acclimatized in the laboratory for 14 days. After that, the rats were grouped into six groups, randomly, which including a healthy control group (K0), a negative control group or hyperlipidemic rats (K-), a positive control group or hyperlipidemia-induced rats that was supplemented with atorvastatin (K+), then a hyperlipidemic rats treated with alcoholic extract of C. chayamansa leaves at doses of 100 mg /kgBB /day for K1, 200 mg/ kgBB/ day for K2 and 400 mg/ KgBB/ day for K3. The results showed that K3 treatment is the most effective and optimum dose for lowering total cholesterol level at 76.81±1.10 mg/dl, triglycerides 72.39±1.66 mg/dl and LDL-C at 21.47±0.58 mg/dl. The aortic histology assessment also showed that the K- group had putative thrombus or plaque in intima, and it was not found in other groups. This research focused on the optimum dose and new usage of C. chayamansa, as an anti-inflammatory in atherosclerosis. By understanding the optimum dose of C. chayamansa, the community can apply and control the herb consumption for their own therapeutic properties. For future application, the herb is potentially developed as anti-atherogenic medicine.]]>
<![CDATA[HERITABILITY ANALYSIS OF LOCAL CORN CULTIVARS FROM KISAR ISLAND SOUTHWEST MALUKU AFTER INDUCED WITH COLCHICINE ]]>
<![CDATA[Sinay, Hermalina]]>;
<![CDATA[Tanrobak, Jonetha]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.19847
<![CDATA[In the plant breeding program, selection is one process which aimed to obtain some varieties with superior traits. Within this process, one of criteria that must be consider is the wide genetic diversity of the plant which was selected, and how these traits are inherited. The purpose of this research was to determine (1) the influence of cultivar differences and the colchicine concentration towards the growth and production of local corn cultivar from Kisar Island Maluku, (2) to determine the heritability of growth and production variables after induced with colchicine. Completely randomized block design was used in this research with factorial design that consist of two factors i.e corn cultivar and colchicine concentration. The research procedure starts from field and seed preparation, immersion of seed into the colchicine solution, transplantation of seed into the field, plant maintenance, and harvesting. The variable measured including growth and production variables of corn. The analysis of variance showed a significant effect of cultivar differences on all growth and production variables (p value > 0.05), while colchicine concentration only showed a significant effect toward the plant height and number of seed per ear. The heritability analysis result showed that all variables observed were in high category. The different corn cultivars have a significant effect on all the growth and production, while the colchicine concentration only affect the plant height and number of seed per ear, and the heritability were in high category. This research provides useful information to farmers how to use colchicine to trigger plant growth and production. It can be used as an information in selecting corn cultivars with high yield potential.]]>
<![CDATA[GROWTH PERFORMANCE, YIELD COMPONENT AND GENOTYPIC RESPONSE OF MUNGBEAN (VIGNARADIATA L.) AGAINST SOIL BORNE DISEASES ]]>
<![CDATA[Uge, Emerensiana]]>;
<![CDATA[Iswanto, Rudi]]>;
<![CDATA[Hapsari, Ratri Tri]]>;
<![CDATA[Trustinah, Trustinah]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23827
<![CDATA[Soil-borne disease caused by fungus infection is one of the disease that causes the death and crop failure of mungbean. The aim of this study was to identify the performance, yield component and respons of plant to the disease. Performance of 25 genotypes were observed in installations research and assessment of agriculture technology (IPPTP), Jambegede of Indonesian Legumes and Tuber Crops Research Institute (ILETRI)at dry season 2018 using RCBD with three replications. The result showed that each variety has different characteristic. The plant with the highest number of plant height was genotype 15 and 18, while the total branches of 25 genotypes were generally similar (4).The highest number of grown and harvested plant was genotype 15. The genotypes with shorter flowering, maturity and harvesting age were 23, 20, 18, 15 and 10. The highest average number of filled pods was genotype 11 and empty pods was genotype 13. There were 8,10 and 2 genotypes with seeds weight of >7, >6 and <6 respectively. The highest yields were obtained from genotype 19 and 3 by total of yield of 2.0 t/ha and 1,9 ton/ha respectively. Resistance test against soil-borne diseases resulted 5 genotypes that were moderate resistant observed at 2 DAP. This research contributed to gave information about new genotypes of mungbean that potentially produce new superior varieties.]]>
<![CDATA[EFFECT OF INDUCED POLYPLOIDY ON PLANT GROWTH, CHLOROPHYLL AND FLAVONOID CONTENT OF ARTEMISIA CINA ]]>
<![CDATA[Kasmiyati, Sri]]>;
<![CDATA[Kristiani, Elizabeth Betty Elok]]>;
<![CDATA[Herawati, Maria Marina]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.22548
<![CDATA[Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be useful information to develop A. cina to become a medicinal plant.]]>
<![CDATA[The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation ]]>
<![CDATA[Mahfudhah, Raudhah]]>;
<![CDATA[Eriani, Kartini]]>;
<![CDATA[Muchlisin, Zainal Abidin]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.22353
<![CDATA[The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.]]>
<![CDATA[MICROBIOLOGICAL QUALITY AND PROXIMATE COMPOSITION OF BAKASANG LAOR, A TRADITIONAL FERMENTED FISHERY PRODUCT IN MALUKU ]]>
<![CDATA[Mahulette, Ferymon]]>;
<![CDATA[Kurnia, Tri Santi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23356
<![CDATA[Bakasang laor is one of the traditional fermented fishery products in Maluku, Indonesia. There are two types of bakasang laor, i.e. with and without vinegar. The microbiological research of bakasang processed use laor as raw material has never been done before. The research aimed to analyze the microbiological quality and proximate composition of two types of bakasang laor. The proximate composition can determine the nutritional value of this product for consumption by the community. The sample of bakasang laor was taken from traditional producer in Latuhalat village, Ambon. The total number of halotolerant and coliform bacteria in bakasang laor without vinegar were 1.7x106 CFU/g and 4.1x106 CFU/g, respectively, while the total of lactic acid bacteria in bakasang laor with vinegar was 6.0x106 CFU/g. The carbohydrate, protein, fat and ash contents of bakasang laor with vinegar was higher than bakasang laor without vinegar. The carbohydrate, protein and fat contents of bakasang laor with vinegar were 19.64 %, 16.23 %, and 2.42 %, respectively. Generally, microbiological quality and proximate composition of bakasang laor with vinegar was better than bakasang laor without vinegar. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various food products. The results of this research to improve the quality of this fermentation product in the future.]]>
