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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Bifidobacterium longum, a Predominant Bifidobacterium in Early-life Infant Potentially Used as Probiotic Dyah Fitri Kusharyati; Anwar Rovik; Dini Ryandini; Hendro Pramono
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25895

Abstract

In early life, Bifidobacteria are reported as dominant bacteria in the human digestive tract. Bifidobacterium is potential as a probiotic. The probiotic property of Bifidobacterium is strain-specific. This study aimed to identify the Bifidobacterium (isolated from less than one-month-old healthy infant stool that potentially used as probiotic) based on the 16S rRNA gene and determining their similarities among Bifidobacteria. The probiotic-potentially Bifidobacterium was re-characterized by performing a Gram’s staining and catalase test. The DNA extraction process was followed by the 16S rRNA amplification using 27F-1492R primers. Sequence similarity was checked by using the BLAST program in the GenBank. The phylogenetic tree was constructed by using a neighbor-joining (NJ) method within the MEGA version 7.0 package. The 16S rRNA gene was presented at 1,500 bp length. Bifidobacterium strains have a 91.14-94.26 % sequence similarity to B. longum subsp. longum strain CCUG30698 which is considered as insufficient for species and genus identifications. However, those isolates could be assigned in a phylogenetic position. This present study suggested the B. longum as the dominant strain of Bifidobacterium in the gut of early-life infants which has potential as a probiotic and is considered as an ideal probiotic for human consumption. This study is useful as basic information for other related research, as well as its application in industrial or community service fields.
Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi' Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Abstract

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.
Characterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic Markers Andi Madihah Manggabarani; Tatik Chikmawati; Alex Hartana
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12221

Abstract

Rambutan (Nephelium lappaceum) is an economically important plant which is native to Indonesia and Malaysia. The diversity of rambutan in Indonesia is abundance especially in Kalimantan where the wild relatives still grow naturally. Rambutan cultivars are usually differed from each other based on fruit morphological characters. However, rambutan tree begins to fruiting for the first time in 3-4 years. Therefore, another character is needed to characterize each cultivar in a short period. The objectives of this study were to distinguish rambutan cultivars using leaf morphological and Inter-Simple Sequence Repeat (ISSR). As many as 30 rambutan cultivars collected from Cipaku Orchard and Mekar Sari Park were observed for their morphological and ISSR characters. Six characters of were surveyed for leaf morphological character. For the genetic character, 6 out of 31 ISSR primers were assessed which resulted in 58 polymorphic bands (87%). As a result, leaf morphological characters overlapped among cultivars causing difficulties distinguishing each cultivar. ISSR marker, three major clusters have been identified according to UPGMA method. Index similarity among rambutan accessions from ISSR data ranged from 48-93%. As a conclusion, ISSR marker could be potentially applied rambutan cultivars characterization.
Pengaruh Pemberian Omega-3, Omega-6, dan Kolesterol Sintetis terhadap Kualitas Reproduksi Burung Puyuh Jantan Wiwi Isnaeni; Abyadul Fitriyah; Ning Setiati
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1150

Abstract

The aims of this research were to study the effect of using the omega-3 (O-3), omega-6(O-6), and synthetic cholesterol (Ch) on the reproductive quality of the male quails.Samples of this research were 35 of 4 weeks old male quails with 60 to 98 g of weight.The samples were grouped randomly into 7 groups, that are R-0 (control-group), R-1(0,163 mg O-3/100 g body weight), R-2 (0,326 mg O-3/100 g bw), R-3 (0,163 mgO-6/100 gbw), R-4 (0,326 mg O6/100 g bw), R-5 (20 mg Ch/100 g bw), and R-6 (40mg Ch/100 g bw). The treatment was given per oral in three periods with 5 days ofeach. there was a week intervening between periods. During the research, samples weregifted rations a basalic-woof (basalic-feed) for twice/day, at 7:00 and 16:00 o’clock. theamount of basalic-woof were 20 g of feed/100 g body weight/day. Water administeredin an ad libitum manner. This research was implemented during 8 weeks. The variableswere the level of testosterone, blood qualities, voice-qualities, color brightness of chestfur, and testis size. The voice quality data were analyzed by one-way ANOVA continuedby orthogonal contrast test. The other data were analyzed by descriptive statistic. Theresult of this research confi rmed that synthetic omega-3 and omega-6 (0,326 mg/100 gbw) and synthetic cholesterol (20 mg/100 g bw) could increase the reproductive qualityof the male quails.Keywords: synthetic cholesterol, synthetic omega-3, synthetic omega-6,reproductive quality
Chromium Phytoremediation of Tannery Wastewater using Ceratophyllum demersum Yani Suryani; Tri Cahyanto; Toni Sudjarwo; Diana Vici Panjaitan; Epa Paujiah; Mohamad Jaenudin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8716

Abstract

Tanning industry produces liquid waste containing heavy metals, especially chromium – harmful for ecosystems and human health. Phytoremediation is a technique that utilizes the physiologicalpotential of plants to transform contaminants to be less or non-hazardous. The aim of the research is to determine the efficiency of Ceratophyllum demersum L. to remediate chrome in tannery wastewater. The research used 84 strands of C. demersum compound leaves of 30 g wet weight. The treatments consist of the use of 7.74 mg/L, 11.30 mg/L, 17.00 mg/L and 23.73 mg/L concentrations of chromium and a control. The research was conducted using a static method. The design of the experiment was the complete random design with 3 replication of treatment in 14 days. The parameters observed were the efficiency of chromium phytoremediation, water turbidity, BOD, and total chlorophyll level of the leaves. The results showed that the highest efficiency was at the concentration of 7.74 mg/L with 1.7% chromium, 17.3% water turbidity, and 46% BOD. Meanwhile, the highest efficiency of total chlorophyll level was 3.88 mg/L, reached at the concentration of 17.00 mg/L. In conclusion, C. demersum is good to use as a phytoremediator of tannery wastewater at the concentration of 7.74 mg/L, subsequently, these results can be used as a basis for the consideration of the application implementation in the process of liquid waste reduction.
Aplikasi Pupuk Kandang Kotoran Ayam pada Tanaman Kacang Tanah (Arachis Hypogeae L.) Neni Marlina; Raden Iin Siti Aminah; Rosmiah -; Lusdi Ramlan Setel
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3957

Abstract

Pupuk kandang kotoran ayam diharapkan dapat memperbaiki sifat fisik, kimia dan biologi tanah, sehingga dapat menyuburkan tanah dan membantu dalam menyumbangkan unsur hara yang dapat digunakan dalam meningkatkan hasil kacang tanah.  Penelitian ini bertujuan untuk mendapatkan takaran pupuk kandang kotoran ayam yang tepat dalam meningkatkan produksi tanaman kacang tanah. Penelitian ini telah dilaksanakan di kebun petani di Desa Payakabung Kecamatan Indralaya Utara Kabupaten Ogan Ilir dari bulan Januari sampai dengan April  2014. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) dengan tiga perlakuan dan delapan kelompok, sehingga berjumlah 24 petak penelitian dan setiap petak diambil 10 tanaman sebagai sampel .  Perlakuannya adalah takaran pupuk kandang kotoran ayam 5, 10 dan 15 ton ha-1.  Hasil penelitian menunjukkan bahwa takaran pupuk kandang kotoran ayam sebanyak 10 ton ha-1 memberikan pertumbuhan dan produksi terbaik dengan ditunjukkan produksi per petak sebesar 2,73 kg petak-1.Poultry manure is expected to improve soil physical, chemical and biological properties. It can improve soil fertility and help in nutrients contribution that can be used to increase the yield of peanut. This study aimed to get the right dose of poultry manure fertilizer in increasing the production of ground peanut plants. This research was conducted in farmyard in the North Indralaya Payakabung District of Ogan Ilir from January to April 2014. The design used in this study was a randomized block design with three treatments and eight groups, thus consisting 24 research plots and each plot was taken as a sample of 10 plants. The treatments of poultry manure fertilizer rate 5, 10 and 15 ton ha-1. The results showed that poultry manure fertilizer rate as much as 10 tons ha-1 gave the best growth and production of 2.73 kg per plot.
Characteristics and Antioxidant Activity of Kebar Grass (Biophytum petersianum) Extract Aminudin Aminudin; Nuri Andarwulan; Nurheni Sri Palupi; Iis Arifiantini
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23820

Abstract

Kebar grass (Biophytum petersianum Klotzsch.) has long been known by the locals of West Papua as medicinal plant that improves fertility. The objectives of this research were to identify and determine the antioxidant activity of kebar grass crude extract through conventional method and to analyze for its antioxidant activity. Extraction method used was conventional method, performed by mixing dry kebar grass with water and boiled until the volume shrunk to 1/3 of initial volume. Analized parameters were crude extract yield, qualitative phytochemical, total phenol and antioxidant activity. Research result showed extract crude yield value of 18.32±0.01%. Qualitative phytochemical contents were dominated by flavonoid, tannin, and saponin. Total phenolic content (TPC) of crude extract was 147±1.24 mgGAE/gdw (0.688±0.01 mgGAE/gfw). Crude extract antioxidant parameter was measured as the value of inhibition, AEAC, TEAC, and IC50 where each value respectively was as followed 42.0±0.047%, 69.93±2.19 mgAE/gdw (8.30±0.26 mgAE/gfw), 65.70±1.54 mgTE/gdw (7.80±0.18 mgTE/gfw) and 0.129±0.003 mgdw (257.75±4.90 mg/L). The contribution of kebar grass TPC content to antioxidant activity is 47.38% (equal to ascorbic acid) and 44.51% (equal to trolox) which means that almost half of kebar grass crude extract’s phenolic compound act as antioxidant. The results of this research can show that the kebar grass has potential as a source of multiple antioxidants, which acts as an analogue of vitamin C and vitamin E.  The antioxidant activity of the kebar grass extract can be the scientific basis for the use of kebar grass as a medicinal plant for infertility problems (curative) and as a functional food ingredient for antioxidant sources that prevent a decrease in reproductive performance (preventive).
Indigenous Knowledge Degradation of Lom community, Bangka Island in identifying and using Pelawan Padang (Tristaniopsis merguensis) Salpa Hartanto; Yohana C Sulistyaningsih; Eko Baroto Walujo
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.14089

Abstract

Pelawan padang (Tristaniopsis merguensis) plays an important role in providing daily needs including goods and services for Lom community in Bangka island. Recently,  modernization has an impact on the indigenous knowledge related to plants utilization in Lom community, especially pelawan padang. The aims of the study were to analyze the roles and benefits and to determine ethnobotanical knowledge changes of Lom people on the use of pelawan padang. Data were collected through interviews using a structured questionnaire. Pebble Distribution Method (PDM) was used to analyze the quantitative data, while the qualitative data were analyzed using descriptive statistic. The result showed that pelawan padang can be used for material construction, firewood, honey production, traditional medicine and material of bagan traditional stilt houses. The degradation of indigenous knowledge of Lom people related to natural resources such as pelawan padang could be assessed according to individual factors which were; age classes and occupation. More respondents aged over 40 years showed the ability in identifying and using Pelawan padang compared to the younger (25-40 years old). According to occupational group,  there was higher number of unemployed respondents and respondents working as farmers that used pelawan padang compared to respondents working as employee. No difference was found for tribal leaders and community members regarding to the knowledge of Pelawan padang benefits. This study provides the latest information about the benefits of plants from the Lom Community that can be developed through the further research, especially for the benefits pelawan padang as a drug.
Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition Enni Suwarsi Rahayu; Noor Aini Habibah; Lina Herlina
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3538

Abstract

Carica pubescens Lenne K. Koch  is a rare species that need to be conserved.  The research aim was to develop a slow growth method of in vitro conservation medium through determining some effects of nutrition decreasing availability in the conservation medium on growth and survival of explants. Establishing epicotyls reached from in vitro seed germination was grown on diluted basic medium of 75% MS (Murashige and Skoog), 50% MS, 25% MS, while osmoregulator compound of mannitol and sorbitol was added to the full MS medium in several concentrations. The treatments were arranged in a completely randomized design with three replications. The epicotyls were grown at storage medium for 12 and 16 weeks, then their survival were evaluated at regeneration medium and rooting medium.  The diluted basic medium and osmoregulator addition were evaluated for its influence in retarding the culture growth in terms of improved survival over the period of 16 weeks.  Data analyzed by one way analysis of variance and Duncan’s multiple range test. The results showed that the decreasing of nutrition concentration suppressed the growth of the epicotyls until 16 weeks after conservation. Epicotyls taken from 16 weeks after conservation could grow on the regeneration medium. The best  survival  was shown by the 75% MS, 50% MS and supplementing of  20 g/l mannitol treatments. Based on these results, 50% MS medium  is recommended  for storage C. pubescens for 16 weeks with no sub-culture.Carica pubescens Lenne K. Koch (karika dieng) merupakan tanaman yang langka sehingga perlu dilestarikan. Penelitian ini bertujuan untuk memperoleh medium penyimpanan in vitro dengan teknik pertumbuhan minimal dengan mengamati pengaruh penurunan ketersediaan nutrisi dalam medium terhadap penurunan pertumbuhan dan daya tumbuh eksplan.Eksplan berupa epikotil kecambah in vitro. Perlakuan penurunan ketersediaan nutrisi dilakukan melalui reduksi konsentrasi nutrisi dari medium Murashige Skoog (MS) dan penambahan osmoregulator (manitol dan sorbitol) dengan berbagai konsentrasi. Penelitian dilakukan dengan rancangan acak lengkap satu faktor dengan tiga ulangan. Epikotil dipelihara dalam medium penyimpanan selama 12 dan 16 minggu, kemudian dievaluasi daya tumbuhnya dengan memelihara dalam medium regenerasi dan medium pengakaran. Data dianalisis dengan analisis varians satu arah dan uji Duncan. Hasil penelitian menunjukkan bahwa penurunan kecepatan penyerapan nutrisi berpengaruh terhadap pertumbuhan eksplan. Epikotil yang telah disimpan selama 12 minggu dan 16 minggu dan ditumbuhkan kembali pada medium regenerasi masih dapat tumbuh dengan intensitas tertinggi pada perlakuan pengenceran 50% MS dan 75% MS, serta penambahan manitol 20 g/l. Komposisi medium ini dapat dimanfaatkan untuk penyimpanan karika dieng selama 16 minggu tanpa sub-kultur. Konsentrasi medium MS 50% direkomendasikan untuk digunakan dalam penyimpanan C. pubescens selama 16 minggu tanpa sub-kultur.
Diversity of Species and Conservation Priority of Butterfly at Suranadi Natural Park of West Lombok, Indonesia Mohammad Liwa Ilhamdi; Agil Al Idrus; Didik Santoso
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10695

Abstract

Butterflies play an important role in the ecosystem of Suranadi Natural Park in West Lombok. Butterflies help preserving the existence and diversity of flora by facilitating the process of pollination so it is crucial for flowering plants. The present study aimed at analyzing the diversity and determining the priority of butterfly conservation at Suranadi Natural Park of West Lombok. This exploration is a descriptive study. Data were observed in four-time repetition in the morning and in the afternoon for two months. The sweeping net technique following observation path was employed in this research (line left, line right,  line central and line waterway). The data analysis used the Shannon-Wiener diversity index and priority for conservation determined by Ministry of Forestry Regulation Number: P.57 / Menhut-II / 2008. Fourty (40) species of butterflies belonging to 5 families identified. The diversity index (H ') of butterflies ranges from 2.63 to 3.43 (medium-high). The two species of the Papilionidae family found at TWA Suranadi, namely, Troides helena and Papilio memnon were the priority of conservation. This research would be helpful to consider conservation strategy and ecotourism.

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