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Molekul: Jurnal Ilmiah Kimia

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Published by Universitas Jenderal Soedirman

ISSN : 19079761 EISSN : 25030310 DOI : -

Core Subject : Science, Engineering,

Chemical Engineering, Chemistry & Bioengineering Chemistry

MOLEKUL is a peer-reviewed journal of chemistry published by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Indonesia. Publishing frequency 2 issues per year, on May and November. This Journal encompasses all branches of chemistry and its sub-disciplines including Pharmaceutical, Biological activities of Synthetic Drugs, Environmental Chemistry, Biochemistry, Polymer Chemistry, Petroleum Chemistry, and Agricultural Chemistry.

Arjuna Subject : -

Articles 331 Documents

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ISOLASI DAN KARAKTERISASI PROTEASE ALKALIN DARI ISOLAT BAKTERI LIMBAH TERNAK DI EXFARM FAKULTAS PETERNAKAN UNSOED Zusfahair Zusfahair; Puji Lestari; Ari Asnani
Molekul Vol 6, No 1 (2011)
Publisher : Universitas Jenderal Soedirman

Protease is one of the widely used enzymes for the industry. The potential resource of microorganism that produced protease is milk cow waste. In this research, isolation and characterization has been done toward isolated protease from milk cow waste of the Exfarm’s Animal Husbandry Faculty at University of Jenderal Soedirman, Purwokerto. The research used experiment method and the parameters observed were the genus of bacteria which produce protease and the activity of protease. The characterizations of protease were determination of optimum pH and temperature, the influence of metal ions, EDTA, surfactant, and commercial detergent toward enzyme activity, and also the study of enzyme stability. The results from the research showed that the isolated bacteria from the Exfarm’s of Animal Husbandry Faculty of UNSOED, which produced protease was Salmonella sp. Characterization of isolated Salmonella sp. from 45% ammonium sulphate fraction indicated that the optimum temperature was 50 ºC, optimum pH was 8, the enzyme was activated by Ca2+ dan Mg2+ ion, whereas it was inhibited by Zn2+, Cu2+ ions and EDTA. The addition of Tween-80 with the concentration of 0.2% and 0.4% increased protease activity, however the addition of Tween-80 with concentration higher than 0.6% decreased the protease activity. Enzyme protease from isolated Salmonella sp. was relatively stable with the addition of commercial detergent such as Attack, Surf, and Bukrim.

SINTESIS DAN KARAKTERISASI SABUN NATRIUM DARI MINYAK BIJI NYAMPLUNG (Calophyllum inophyllum L.) SERTA UJI AKTIVITAS ANTIBAKTERI TERHADAP Staphilococus aureus Mochamad Chasani; Senny Widyaningsih; Adidyan Mubarok
Molekul Vol 10, No 1 (2015)
Publisher : Universitas Jenderal Soedirman

Penelitian ini menggunakan minyak biji nyamplung sebagai bahan dasar sabun antibakteri. Pembuatan sabun dari minyak biji nyamplung dilakukan dengan beberapa tahap yaitu sintesis sabun menggunakan metode saponifikasi dan karakterisasi sabun berdasarkan SNI 06-3532-1994. Untuk mengetahui sifat antibakteri sabun, dilakukan uji aktivitas antibakteri terhadap bakteri Staphylococus aureus. Hasil penelitian menghasilkan sabun yang berwarna kuning dengan berat 13,028 g dari 10,028 g minyak biji nyamplung. Karakteristik sabun berdasarkan SNI didapatkan kadar air 25,287%, jumlah asam lemak 72,177%, alkali bebas 0,082%, lemak yang tidak tersabunkan atau lemak netral 0,834% dan minyak mineral negatif. Sabun berbahan dasar minyak biji nyamplung memiliki aktivitas antibakteri terhadapStaphylococus aureus dengan diameter zona bening sebesar 14,701 mm.

Activity of Superoxide Dismutase Mimic of [Mn(salen)OAc] Complex Compound Non-enzymatically in Vitro Through Riboflavin Photoreduction Yusi Deawati; Djulia Onggo; Irma Mulyani; Iwan Hastiawan; Dikdik Kurnia
Molekul Vol 12, No 1 (2017)
Publisher : Universitas Jenderal Soedirman

The complex compound of [Mn(salen)OAc] can serve as mSOD and its activity has been determined non-enzymatically in vitro through riboflavin photoreduction. The complex was synthesized from Mn(OAc)2.4H2O and H2salen. Based on the elemental analysis, the C=56.69%; H=4.21%; and N=7.52% content are corresponding to the chemical formula of MnC18H17N2O4. The functional groups and ionic species in the complex have been analyzed by infrared spectroscopy and ESI-MS. SOD activity was determined by mixing complex at various concentrations with riboflavin and nitroblue tetrazolium (NBT), then the mixture was lighted with 20 Watt tungsten lamp for 15 minutes in a closed box. Then the reduced NBT absorptions were measured at Î» 560 nm. The difference of absorbance between standard and sample solutions (without and with riboflavin, respectively) was multiplied by 100% to obtain %inhibition of each various sample concentration against NBT. SOD activity was obtained from IC50 data defined as a 50% inhibition of the plot curve of % inhibition to the concentration of the complex. The result obtained for this compound is IC50 = 2.7 Â± 0.05 ÂµM as well as enzymatic method. Therefore, this method can be used to determine the SOD activity by giving more stability and accuracy of IC50 value.

PENGOLAHAN LIMBAH CAIR INDUSTRI TAHU DI DESA KALISARI KECAMATAN CILONGOK DENGAN METODE MULTI SOIL LAYERING Irmanto Irmanto; Suyata Suyata
Molekul Vol 4, No 1 (2009)
Publisher : Universitas Jenderal Soedirman

Tofu industry is one of the industries which causing pollution on the environment especially water environment. The amount of wastewater-dumped and the content of organic substances could damage the water ecosystem. Multi Soil Layering (MSL) as one of alternative method is used in order to remove the tofu industrial wastewater pollution. MSL is a method of wastewater treatment that enhances the function of soils to purify wastewater.The research aimed to determine optimum loading rate and efficiency of MSL system to remove TSS, BOD and COD. The wastewater on the MSL system is loaded at the loading rate varieties, i.e: 160, 320, 480, 640, and 800 lm-2 day-1, and the aeration was applied for 8 a day at 1296 ld-1.The results revealed that optimum loading rate was 320 lm-2 day-1 and efficiency of MSL system to remove TSS, BOD and COD were 78.62 % ; 98,89% and 95,53 % respectively.

DEODORISASI LIMBAH CAIR BATIK MENGGUNAKAN LIMBAH BAGLOG Pleurotus ostreatus DENGAN KOMBINASI VOLUME DAN WAKTU INKUBASI BERBEDA Heru Teguh Sumarko; Sri Lestari; Ratna Stia Dewi
Molekul Vol 8, No 2 (2013)
Publisher : Universitas Jenderal Soedirman

Limbah cair batik yang dibuang ke lingkungan tanpa diolah terlebih dahulu bersifat toksik, mengakibatkan penurunan kualitas air disekitar lingkungan dan kesehatan dengan munculnya masalah utama seperti bau tidak sedap. Kondisi tersebut diperlukan penanganan agar efek pencemaran rendah atau menjadikan limbah cair batik tidak toksik. Penelitian tentang pengelolaan limbah cair batik berupa deodorisasi menggunakan limbah baglog Pleurotus ostreatus dengan kombinasi volume dan waktu inkubasi berbeda telah diujikan. Tujuan penelitian adalah untuk mengetahui kemampuan limbah baglog P. ostreatusdengan kombinasi volume dan waktu inkubasi berbeda dalam mendeodorisasi limbah cair batik, dan untuk mengetahui perlakuan yang terbaik dalam mendeodorisasi limbah cair batik menggunakan limbah baglog P. ostreatus dengan kombinasi volume dan waktu inkubasi berbeda. Hasil penelitian menunjukkan bahwa limbah baglog P. ostreatus dengan kombinasi volume dan waktu inkubasi berbeda mampu mendeodorisasi limbah cair batik. Hasil terbaik berupa penurunan skala bau 3 (tidak bau) yang diikuti persentase penurunan nilai BOD sebesar 93,95% (3301 mg/l menjadi 200 mg/l) dan COD 79,66% (15200 mg/l menjadi 3120 mg/l) pada perlakuan 100 ml volume limbah cair batik dan 96 jam waktu inkubasi.

ACE Inhibitory and Antioxidative Bioactive Peptides Derived from Hydrolyzed Soy Milk Hermanto, Sandra; Septiana, Annisa; Putera, Deni K; Hatiningsih, Fitriah; Muawanah, Anna
Molekul Vol 14, No 1 (2019)
Publisher : Universitas Jenderal Soedirman

Soy milk is a soybean processed product rich in protein as well as sources of bioactive peptides. Bioactive peptides defined as specific protein fragments that have a positive impact on body functions and conditions and may ultimately influence health. This study was conducted to explore the potential of hydrolyzed soy milk as a source of antioxidative and antihypertensive bioactive peptides through enzymatic hydrolysis. The initial treatment of soy milk protein was acidic precipitation with hydrochloric acid. Furthermore, protein precipitate was hydrolyzed using pepsin proteolytic enzyme with an enzyme: substrate ratio (1:5, 1:10 and 1:20). Protein hydrolysis was carried out for 0?48 hours at 37 °C in an acetate buffer pH 4.5. The soy milk protein hydrolysates were subjected to determination of % DH (Degree of Hydrolysis) and protein profile by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The antihypertensive assay was carried out by in vitro inhibition of the ACE enzyme (Angiotensin Converting Enzyme) and antioxidative activity assay using the DPPH method. The results showed that the optimum conditions for hydrolysis of soy milk were obtained at 40 hours with a % DH value of 53.24% in enzyme ratio 1:20 and the highest antihypertensive activity was obtained from 48 hours hydrolysis with % ACE inhibition value of 79.31%. The highest antioxidative activity of bioactive peptide was obtained at hydrolysis 48 hours with IC50 69.1034 µg / ml. After fractionated and LCMS characterized it was obtained 2 bioactive peptides with molecular weights of 8.954 and 2,696 kDa. These bioactive peptides from hydrolyzed of soymilk might be potential as an antihypertensive agent and reduce oxidative stress.

Histochemical Changes Liver and Kidney of Mice Exposed to Mercury and Recovery with Nanogold Titik Taufikurohmah; I Gusti Made Sanjaya; Afaf Baktir; Achmad Syahrani
Molekul Vol 11, No 1 (2016)
Publisher : Universitas Jenderal Soedirman

The background of this research is the circulation cosmetic with mercury that occur today in society. The problem of the research is that occur histochemical’s damage liver and kidney after exposure to mercury, and is that nanogold can recovery that damage. The pre-clinical study needed 24 mice (Mus muscullus) were divided into 6 groups, the control is A group, B group was exposed to mercury, Groups C, D, E and F after being exposed to mercury, than recovery by nanogold with concentration each of 5, 10, 15 and 20 ppm. Exposure was performed 1 week and 4 weeks of recovery. Necropsy of mice doing after treatment, liver and kidneys are processed into preparations by blocking with paraffin embedding method. Histochemical staining of liver and kidney tissue with Hematoxylin eosin (HE) to determine changes of cell constituent and staining Van Geyson to determine the structure of collagen constituent. Statistics Manova showed different results between treatment groups. Tissue damage, lysis cell and destruction of collagen can be observed from histochemical techniques for mercury-exposed group compared to the control group. Tissue and collagen recovery process can be observed from group C, D, E and F. The conclusion that the effects of mercury one week exposed through skin give effect to collagen tissue damage at liver and kidneys of mice. 20 ppm of Nanogold can recovery damaged cells and collagen tissue from the liver and kidneys of mice after four weeks of recovery.

PENENTUAN ASAM OKSALAT SECARA SPEKTROFOTOMETRI DENGAN METODE METILEN BIRU Irmanto Irmanto; Suyata Suyata
Molekul Vol 1, No 1 (2006)
Publisher : Universitas Jenderal Soedirman

A spectrophotometric method for the determination of oxalic acid has been developed, based on its catalytic effect on the redox reaction between methylene blue and sulphide, measured at the maximum absorption wavelength of 664 nm. Under the optimum conditions of 3 mL of 31 % formaldehyde; 0.8 mL of 0,067 M EDTA - Triethanolamine; 1.2 mL of 0,010 M sulphide; 2 mL of 0,0010 % methylene blue at 20 oC for 1 minute a detection limit of 0,43 mg/mL for oxalic acid determination was obtained. This method can be applied to determination of trace level of oxalic acid.

PEMBUATAN DEKSTRIN DARI PATI UBI KAYU MENGGUNAKAN KATALIS AMILASE HASIL FRAKSINASI DARI Azospirillum sp. JG3PEMBUATAN DEKSTRIN DARI PATI UBI KAYU MENGGUNAKAN KATALIS AMILASE HASIL FRAKSINASI DARI Azospirillum sp. JG3 Zusfahair Zusfahair; Dian Riana Ningsih
Molekul Vol 7, No 1 (2012)
Publisher : Universitas Jenderal Soedirman

Amilase merupakan salah satu enzim potensial dalam proses industri. Amilase dapat diperoleh dari bakteriAzopsirillum sp. JG3. Amilase ini dapat dimanfaatkan untuk hidrolisis pati ubi kayu pada pembuatan dekstrin. Dekstrin memiliki peran yang cukup penting dalam industri seperti pembuatan roti, makanan bayi dan bahan penyalut lapis tipis tablet. Penelitian ini bertujuan untuk mengetahui karakterisasi biokimiawi amilase hasil fraksinasi dari Azospirillum sp. JG3 serta potensinya dalam pembuatan dekstrin.Tahap penelitian ini dimulai dengan memproduksi enzim yang dilakukan dengan cara menumbuhkan inokulum ke dalam medium produksi selama 30 jam kemudian diekstraksi dengan sentrifugasi sehingga diperoleh ekstrak kasar. Ekstrak kasar amilase kemudian difraksinasi secara bertahap (15%, 30%, 45% dan 60%) dengan menggunakan ammonium sulfat dan didialisis. Fraksi yang diperoleh diukur aktivitas, volume dan kadar proteinnya. Fraksi dengan aktivitas spesifik tertinggi dikarakterisasi dan digunakan untuk membuat dekstrin dari pati ubi kayu. Dekstrin pati ubi kayu yang diperoleh dikarakterisasi berupa rendemen, kadar air, kadar abu dandextrose equivalen.Hasil penelitian menunjukkan bahwa fraksi hasil dialisis 45% (FHD 45%) menghasilkan aktivitas dan aktivitas spesifik tertinggi berturut-turut sebesar 11,659 U/mL dan 7,319 U/mg. Aktivitas amilase optimum pada suhu 30°C, pH 7 dan konsentrasi substrat 4%. Dekstrin yang diperoleh memiliki rendemen 95,14, kadar air 3,411%, kadar abu 0,209% dan dextrose equivalen 13,4.

Degradation of Paracetamol by Photolysis Using C-N-codoped TiO2 Vanny Yulia Safitri; Adlis Santoni; Diana Vanda Wellia; Khoiriah Khoiriah; Safni Safni
Molekul Vol 12, No 2 (2017)
Publisher : Universitas Jenderal Soedirman

Paracetamol is generally used as analgesic and antipyretic drugs. Contamination paracetamol in the environment can occur because of waste material disposal from production site and immediate disposal of household that cause water pollution. Paracetamol is degraded by photolysis method under irradiation 10 watt UV-light (λ=365 nm), visible-light (Philips LED 13 watt 1400 lux) and solar-light with and without addition C-N-codoped TiO2catalyst. The solution is analyzed by UV-Vis spectrophotometer at λ 200-400 nm. Optimum weight of C-N-codoped TiO2 catalyst obtained is 20 mg under UV-light photolysis. Paracetamol 4 mg/L is degraded 45.48% after 120 minutes under UV-light irradiation without catalyst, and increases to be 69.31% by using 20 mg catalyst. While degradation percentage of paracetamol is 16.96 % without catalyst, the percentage increases to be 34.29% after using 20 mg catalyst for 120 minutes photolysis under visible-light. Degradation of paracetamol by solar light achieves only 12.27% in absance of catalyst for 120 minutes irradiation, but it increases significantly until 70.39% in presence of 20 mg catalyst.

Page 16 of 34 | Total Record : 331

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Home Page

OAI Link

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Contact Name Amin Fatoni

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Editorial Address Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

Location Kab. banyumas, Jawa tengah INDONESIA

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Amin Fatoni

Contact Email
aminfatoni@unsoed.ac.id

Phone
-

Journal Mail Official
j.molekul@gmail.com

Editorial Address
Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

Location
Kab. banyumas,

Jawa tengah

INDONESIA