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Jurnal Farmasi Indonesia yang diterbitkan oleh Pengurus Pusat Ikatan Apoteker Indonesia. Isi website memuat seluruh jurnal yang telah diterbitkan mencakup semua aspek dalam ilmu pengetahuan dan teknologi kefarmasian antara lain farmakologi, farmakognosi, fitokimia,farmasetika, kimia farmasi, biologi molekuler, bioteknologi, farmasi klinik,farmasi komunitas, farmasi pendidikan, dan lain-lain.

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Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i2.166

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effectÂ is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later itÂ was found that even the fixed oil of Nigella sativa has greater anti inflammation effect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development isÂ done to enhance the bioavailability. Nanoemulsion contain 200Î¼L Nigella sativa oilÂ per 1 g mixture of oil phase made by VCO, Cremophor RH40, Â polietilen glikol 400Â rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparentÂ mixture within 9,17 Â± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, meanÂ globules diameter of 57,6 Â± 13.04 nm, polydispersity index of 0,288 Â± 0,033, and zetaÂ potential of 0,243 Â± 0,136 mV. Stability test of centrifuge test showed that SNEDDSÂ is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result ofÂ anti inflammation acute activity test of Nigella sativa oil which is incorporate inÂ SNEDDS of VCO has no antiinflammation. Keywords: Nigella sativa oil, Self Nanoemulsion, anti inflammation Â Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamasi yang cukup kuat. Senyawa yang bertanggung jawab memberikan Â efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak Â menguap dari minyak jintan hitam. Namun ditemukan bahwa Â bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai Â masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi Â spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 Î¼L minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 Â± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 Â± 13.04 nm, indeks polidispersitas Â 0,288 Â± 0,033, Â dan zeta potensial 0,243 Â± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketigaÂ freeze thawmenunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi. Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinflamasi akut Â Â

Aktivitas Antioksidan Daun Iler Plectranthus scutellarioides (L.) R.Br. Moelyono, M W; Rochjana, Anna Uswatun Hasanah; Diantini, Ajeng; Musfiroh, Ida; Sumiwi, Sri Adi; Iskandar, Yoppi; Susilawati, Yasmiwar
Jurnal Farmasi Indonesia Vol 8, No 1 (2016)
Publisher : Jurnal Farmasi Indonesia

ABSTRACT : Antioxidants are the compounds capable to inhibit free radical reactions in the human body. This research was aimed to identify the antioxidant potency of ethanolic extract of Plectranthus scutellaroides leaves in vitro by using spectrophotometric methods with DPPH (1,1-diphenyl-2-picryl hydrazyl) using vitamin C as reference. Concentrations of samples used were 75, 100, 115, 125 and 135 ppm. The antioxidant activity was measured by visible spectrophotometry at three wavelengths of 498, 518 and 538 nm. The result showed that the n-hexane fraction gave the highest antioxidant activity with IC50 of 52.5 ppm, 15 times lower than that of vitamin C (IC50 of 3.33 ppm). Phytochemical screening of the Plectranthus scutellaroides leaves indicated the presence of flavonoids, polyphenolic, monoterpenoids, sesquiterpenoids, steroids and triterpenoids. Keywords: antioxidant, Plectranthus scutellaroides, leaves, DPPH ABSTRAK Antioksidan merupakan senyawa yang dapat menghambat reaksi radikal bebas dalam tubuh. Penelitian ini bertujuan untuk mengetahui daya antioksidan dari daun Plectranthus scutellarioides (L.) R.Br., secara in vitro dengan metode spektrofotometri menggunakan pereaksi 1,1-difenil 2-pikrilhidrazil (DPPH) dengan vitamin C sebagai pembanding. Daun diekstrak menggunakan etanol lalu difraksinasi dengan n-heksana, etil asetat dan air. Variasi konsentrasi sampel uji yang digunakan pada pengujian ini adalah 75, 100, 115, 125 dan 135 ppm. Aktivitas antioksidan diukur secara spektrofotometri pada tiga panjang gelombang yaitu 498, 518 dan 538 nm. Hasil penelitian menunjukkan bahwa fraksi n-heksana pada konsentrasi tersebut memberikan aktivitas antioksidan paling kuat dengan nilai IC sebesar 52.5 ppm, 15 kali lebih lemah dibandingkan dengan vitamin C (IC50 = 3.33 ppm). Hasil penapisan fitokimia terhadap daun Plectranthus scutellaroides menunjukkan adanya senyawa golongan flavonoid, polifenolat, monoterpenoid, sesquiterpenoid, steroid dan tritertenoid. Kata kunci: antioksidan, Plectranthus scutellaroides, daun, DPPH

ANALISIS FAKTOR YANG MEMPENGARUHI KEPATUHAN PENGAMBILAN OBAT PESERTA PROGRAM RUJUK BALIK DI BANDAR LAMPUNG Efayanti, Dwi
Jurnal Farmasi Indonesia Vol 9, No 1 (2017)
Publisher : Jurnal Farmasi Indonesia

ABSTRACT : Program (Program Rujuk Balik = PRB) through a health care program for the participants of the National Health Insurance with chronic diseases. Barriers to success of therapeutic treatment for patients with chronic diseases is non-adherenceÂ related to the patient's treatment regimen. Taking medication adherence for patients with chronic illness is the first adherence can be assessed on how a person decides to get medicines for themselves. Analyzing factors affecting medication adherence taking participants PRB is important, cause non-adherence adverse effects appear in the treatment.Â Participants adherence taking the drug on a PRB can be obtained from the data prescription claims to calculate the medication possession ratio (MPR), which MPR â?¥ 80% declared adherence. Data obtained fromÂ prescription claim by participants PRB forÂ September 2015 until December 2015 in Bandar Lampung. Sample case study 82 people with diabetes. The calculation result MPR explains that 62.2% of respondents declared adherence taking medication, while 37.8% of respondents declared non-adherence. The results of theÂ logistic regression analysis, stating that there are influences together of factors respondents' knowledge, respondents belief, attitudes of personnel pharmacy services, pharmacy facilities, cost, availability of medication PRB, family support, and accessibility affect to respondent adherence ofÂ taking medication. However, factors that significantly influence medication adherence refer the decision back is knowledge of respondents with sig = 0,028 (sig <0.05), belief of respondents with sig = 0,003 (sig <0.05), and accessibility sig = 0,044 (sig < 0.05) significantly affect to adherenceÂ of taking medication.

PENTAGAMAVUNON-1 MENGHAMBAT SIKLUS SEL T47D TERINDUKSI CASPASE INHIBITOR Z-VAD-Fmk PADA FASE G2-M Daâ??i, Muhammad; A.M., Supardjan; Jenie, Umar Anggara; M, Kawaichi; Meiyanto, Edy
Jurnal Farmasi Indonesia Vol 5, No 4 (2011)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i4.54

Previous experiment indicated Curcuminâ??s analogue Pentagamavunon-1 (2,5-bis(41-hidroxy-31,51-dimethyl)-benzilidine-cyclopentanone) has inhibitory activity on T47D cell proliferation through induction of apoptosis and cell cycle arrest at G2-M phase. This research was conducted to observe the relationship between the induction of apoptosis and inhibition of cell cycle in T47D cells induced by Pentagamavunon-1 (PGV-1). The T47D cells were treated with 2.5 PGV-1 mM; Z-VAD-Fmk 2.5 mM; (Z-VAD-Fmk 2.5 mM+PGV-1 2.5 mM). Kinetics of cell proliferation was observed with flowcytometer analysis, molecular expression was observed with Western blot methods. The results showed induction of PGV-1 and Z-VAD-Fmk stimulate the accumulation of cells in G2-M phase (39.28%), did not differ significantly with cells that Â induced by PGV-1 only (34.19%). Molecular analysis showed that treatment with (PGV-1+Z-VAD-Fmk) could prevent apoptosis through inhibition of activation of Caspase-3, increased the expression of p21 and activated Cdc-2. Overall the study showed inhibition of cell cycle at G2M phase by PGV-1 compound is not affected by the activation of caspase-3. ABSTRAK Analog kurkumin Pentagamavunon-1 Â (2,5-bis(41-hidroksi-31,51-dimetil)-benzilidinsiklo-pentanon) telah diteliti dapat menghambat proliferasi sel melalui mekanisme induksi apoptosis dan cell cycle arrest pada fase G2-M. Penelitian ini dilakukan untuk mengamati keterkaitan antara proses induksi apoptosis dan penghambatan siklus sel pada sel T47D yang diinduksi senyawa Pentagamavunon-1 (PGV-1). Sel T47D diberi perlakuan PGV-1 2,5 mM; Z-VAD-Fmk 2,5 mM dan (Z-VAD-Fmk 2,5 mM+PGV-1 2,5 mM). Kinetika proliferasi sel diamati dengan analisis flowcytometric, ekspresi molekuler diamati dengan metode Western blot. Hasil pengamatan menunjukkan induksi (PGV-1+Z-VAD-Fmk) memacu akumulasi sel pada fase G2-M (39,28%) tidak berbeda signifikan dengan sel yang hanya diinduksi PGV-1 (34,19%). Pengamatan molekuler menunjukkan perlakuan (PGV-1+Z-VAD-Fmk) mencegah terjadinya apoptosis melalui penghambatan aktivasi Caspase-3. Secara keseluruhan penelitian menunjukkan penghambatan siklus sel pada fase G2-M oleh senyawa PGV-1 tidak tergantung oleh aktivasi Caspase-3.

EFEK SITOTOKSIK EKSTRAK TANAMAN KELADI TIKUS (Typhonium divaricatum (L.) TERHADAP SEL HeLa Daâ??i, Muhammad; Fiveri, Anis; Meiyanto, Edy
Jurnal Farmasi Indonesia Vol 3, No 4 (2007)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i4.86

Cervic cancer is the most cancer that suffered by women in the world include in Indonesia. The main purposes of this experiment is to explore the Indonesian plan to find new anticancer agent. This experiment exhibit the cytotoxic effect of (Typhonium divaricatum (L.) Decne) on HeLa cells line. This experiment also directed to know the corelation phenolic content with the cytotoxic effect of extracts Typhonium divaricatum. The result indicated ethyl acetate extract had the best potency as cytotoxic agent compared with ethanol and cloroform extract based on MTT method. The IC50 value of ethil acetate extract was 147,77 Î¼g/ml, chloroform extract was 903,44 Î¼g/ml (based on the extrapolation). Ethanol extract was not resulted the IC50 value. Phenol content in each extracts was resulted by Folin-Ciocalteu method. Total phenol content of ethyl acetate, ethanol and chloroform extracts were 13.154; 12.028; 2.872 mg/g extract respectively. Those results indicate there is no corelation between total phenol content and cytotoxic effect direcly of each extracts. ABSTRAK Kanker leher rahim adalah kanker yang banyak diderita wanita diseluruh dunia, termasuk di Indonesia. Selama ini terapi kanker dengan kemoterapi belum memperoleh hasil yang memuaskan. Penelitian ini dilakukan untuk mendapatkan bahan obat dari alam sebagai antikanker dari tanaman Indonesia. Penelitian ini dilakukan untuk menentukan efek sitotoksik keladi tikus (Typhonium divaricatum (L.) Decne) pada sel kanker leher rahim (sel HeLa) dan diarahkan pula untuk mengetahui korelasi kandungan senyawa fenolik terhadap potensi sitotoksisitas ekstrak tanaman keladi tikus. Hasil uji sitotoksik terhadap sel HeLa, ekstrak etil asetat memiliki efek sitotoksik terbesar terhadap sel HeLa dibanding ekstrak kloroform dan etanol. Nilai IC50 ekstrak etil asetat adalah 147,77 Î¼g/ml, kloroform sebesar 903,44 Î¼g/ml (hasil ekstrapolasi), sedangkan harga IC50 ekstrak etanol tidak dapat ditentukan. Penetapan kadar fenol total dalam masing-masing ekstrak ekstrak tanaman keladi tikus diperoleh dengan penyari etil asetat (13,154 mg/g ekstrak) > etanol (12,028 mg/g ekstrak) > kloroform (2,872 mg/g ekstrak). Hasil-hasil tersebut menunjukkan kandungan senyawa fenolik bukan merupakan penanda utama potensi sitotoksik suatu ekstrak.

Efek Antidiabetes Kombinasi Infus Biji Oyong (Luffa Acutangula L.Roxb) dengan Metformin dan Glibenklamid Herowati, Rina; Widodo, Gunawan P; Sulistyani, Putri W; Hapsari, .
Jurnal Farmasi Indonesia Vol 6, No 4 (2013)
Publisher : Jurnal Farmasi Indonesia

Oyong (Luffa acutangula L.Roxb) seed is a traditional medicinal plant containing flavonoids, saponins, polyphenols and tannins which empirically used to treat diabetes mellitus. Combination of natural product along with conventional drugs has found to be beneficial in certain diseases, such as diabetes mellitus. This research aimed to investigate hypoglycemic effect of combination dose of oyong seed infuse and glibenclamide as well as metformin. Diabetic condition on animal model was conducted by induction of Alloxan as well as by glucose tolerance method. The data obtained were analyzed by one way ANOVA (p<0.05). The result showed that lowering blood glucose effect of oyong seed infusion was higher than that of glibenclamide and metformin on Â aloxan induced diabetic mice. Only oyong seed infuse in single administration gave significant lowering effect of blood glucose level on glucose tolerance test.Keywords : Luffa acutangula seeds, alloxan induced diabetic test, glucose tolerance test.

Pengembangan Metode Ekstraksi dan Analisis Irbesartan dalam Plasma Darah menggunakan High Performance Liquid Chromatography (HPLC) Fluorescence Ratih, -
Jurnal Farmasi Indonesia Vol 7, No 3 (2015)
Publisher : Jurnal Farmasi Indonesia

A simple extraction and analysis method by High Performance Liq- uid Chromatography (HPLC) ô°luorescence has developed for determination of Irbesartan in plasma sample. The chromatography system were ô°luorescence detector at Î» ex254nm/em380 nm, ô°low rate 1 mL/min, C-18 150 x 4,6 mm; 5Î¼m column and acetonitrile : 0,5% (v/v) H3PO4 pH 3,0 (77:23) as mobile phase by run time not more than 6 minutes. Reliability of the HPLC method was as- sessed by the linearity of 9 levels concentration which be found 10.0 â?? 5,000.0 ng/mL, r = 0.9996 respectively. Chloroform was used as pretreatment solvent to extract Irbesartan dan Candesartan cilexetil (Internal Standard) from protein binding. The chromatogram of Irbesartan dan Candesartan cilexetil peaks were clearly separated without matrix noises, it was showed by resolution factor > 2. Â

Aktivitas Antidiabetes Ekstrak Etanol Kulit Batang Faloak (Sterculia quadrifida R.Br.) Terhadap Tikus Wistar yang Diinduksi Aloksan Diki Dongga, Irene Rambu Yety; Sunarti, Sunarti; Sunarti, Titik
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Diabetes mellitus is a chronic disease with hyperglycemic and oxidative stress component that can lead to various complications. The stem bark of faloak have been used traditionally to treat diabetes by local people in East Nusa Tenggara. Therefore, this research aimed to study antidiabetic activities in alloxan-induced rats of faloak ethanolic extract. The all rats were divide at random into group I : normal control; groups II : diabetic control; group III : standart (glibenclamide); group IV â?? VI : administrations group of faloak stem bark ethanol extract at a dose of 65 mg / kg, 130 mg / kg and 260 mg / kg for 14 days. Blood samples were collected from rats in duration 0 â?? 14 days for T0, T4, T7 and T14 after alloxan treatments. The results showed that ethanol extract of faloakâ??s bark dose of 260 mg/ kg on 14 days had decreased blood glucose levels 46,07%, no different glibenclamide group. The dose of 65 mg/kg and 130 mg/kg reduced glucose level respectively 26,32 % and 38,09 %.

DISOLUSI KAPSUL TEOFILIN DALAM MODEL RACIKAN RESEP DOKTER Hutagaol, Lungguk; Irwan, Yenny
Jurnal Farmasi Indonesia Vol 5, No 1 (2010)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i1.35

Theophylline is asthma treatment that quite a lot used and have narrow therapy scope and is often combined with ephedrine hidrochloride in doctorâ??s prescription. Recently handling compounding theÂ prescription is Â in highly varied and this condition has influenced dissolution rate. In consequence, conducted research hits difference dissolution capsule from compounding that indigenous to active ingredient and from tablet that triturated become powder. Despitefully, conducted research hits influence ephedrine hydrochloride to theophylline dissolution rate. The result can be concluded that formed compounding from powder produces super ordinate dissolution are compared to compounding from combination ofÂ theophylline and ephedrine hydrochloride tablet. So compounding from theophylline and ephedrine hydrochloride tablet is inadvisable because they decrease theophylline dissolution rate. ABSTRAK Teofilin merupakan obat asma yang cukup banyak digunakan dan mempunyai lingkup terapi Â sempit serta sering dikombinasi dengan efedrin hidroklorida dalam racikan resep dokter. Saat ini penanganan racikan resep dokter di apotekÂ sangatÂ bervariasi dan hal ini berpengaruh pada kerapatan mampat yang akhirnya mempengaruhi kecepatan disolusi. Oleh karena itu dilakukan penelitian mengenai perbedaan disolusi kapsul racikan yang berasal dari bahan baku serbuk dan dari tablet yang digerus menjadi serbuk. Di samping itu, dilakukan penelitian mengenai pengaruh efedrin hidroklorida terhadap disolusi kapsul teofilin. Dari hasil penelitian dapat disimpulkan bahwa kapsul yang diracik dari bahan baku serbuk menghasilkan disolusi yang lebih tinggi dibandingkan dengan kapsul yang diracik dari bentukÂ tablet. Oleh karena itu racikan dari tablet Â teofilin dan efedrin hidrokloridaÂ tidak disarankan karena menurunkan laju disolusi teofilin.

INTERAKSI SIMETIDIN TERHADAP KINETIKA ELIMINASI PARASETAMOL PADA KELINCI Sumarno, .
Jurnal Farmasi Indonesia Vol 3, No 1 (2006)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i1.67

Cimetidine is a H2-antagonist which has potential to interfere the metabolism of other drugs by inhibit the activity of liver microsomal enzymes by binding cytochrome P-450, therefore diminished active cytochrome P-450. The metabolism of paracetamol occurred in liver cells which involve cytochrome P-450 in phase I N-hydroxylation reaction. Â This experiment objective was to investigate the interaction of cymetidine on the kineticÂ of elimination of paracetamol. The experiment done on 2 groups of rabbit, 5 in control group and the other 5 in the treatment group. Rabbit in control group was only given paracetamol 450 mg/kg per oral, while the treatment group was given cymetidine 78 mg/kg per oral every 6 hour for three times one day before the administration of paracetamol. Rsults showed that there was significant differences in kinetic of elimination reaction of paracetamol between the two groups (p < 0,01). Half life (t1/2b) of paracetamol elimination Â in control group was 161,02 minutes, while in pre-treatment group was increase to 429,26 minutes. It was concluded that cymetidine could interfere, i.e. lenghthen the half life elimination of paracetamol. ABSTRAK Simetidin merupakan salah satu H2-antagonist yang poten menghambat metabolisme obat lain, yang bekerjanya menghambat aktivitas enzim mikrosomal hati dengan cara mengikat sitokrom P-450 yang sifatnya reversible membentuk kompleks, sehingga sitokrom P-450 aktif berkurang jumlahnya. Metabolisme parasetamol terjadi di hati yang juga melibatkan sitokrom P-450 dalam metabolisme fase pertama pada reaksi oksidasi N-hidroksilasi. Penelitian interaksi simetidin terhadap kinetika eliminasi parasetamol dilakukan pada 2 kelompok kelinci yang terdiri dari 5 ekor kelompok kontrol dan 5 ekor kelompok perlakuan. Kelompok kontrol hanya diberi parasetamol oral 450 mg/kg BB, sedangkan kelompok perlakuan diberi simetidin oral 78 mg/kg BB setiap 6 jam sebanyak tiga kali sehari sebelum pemberian parasetamol. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang bermakna pada kinetika eliminasi parasetamol setelah praperlakuan simetidin (p < 0,01). Hal ini terlihat bahwa waktu paruh elminasi parasetamol (t1/2b) pada kelompok kontrolÂ sebesar 161,02 menit , sedangkan pada kelompok perlakuan meningkat menjadi 429,26 menit. Tetapan laju eliminasi parasetamol (b) pada kelompok kontrolÂ sebesar 4,82.10-3 menit-1, sedangkan pada kelompok perlakuan menurun menjadiÂ 1,69.10-3 menit-1. Hal ini menunjukkan adanya pengaruh inhibisi simetidin terhadap metabolisme parasetamol sehingga waktu paruh eliminasi parasetamol menjadi lebih panjang.

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