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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 176 Documents

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PETUNJUK PENULIS dan DAFTAR ISI Romanus Edy Prabowo
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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PERBEDAAN PROFIL LIPID DAN KADAR ASAM URAT PADA PASIEN DIABETES MELITUS TIPE II DENGAN DAN TANPA HIPERTENSI Filliana Savitri; Intani Kurnia Savitri; Pugud Samodro; Lantip Rujito
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Diabetes Mellitus type 2 is the 7th world of death problem with the 95% proportion rate. Hypertension has also a correlation with this condition. The mortality of cardiovascular is 2-3 times over from diabetics with hypertension than diabetics with normotension which caused by lipid and aciduric disturbations. This study was conducted using cross-sectional design. Twenty-five sample of diabetics normotension and 25 sample of diabetics patient along with hypertension in Ajibarang Hospital District Banyumas and comply with inclusion-exclusion criteria were taken suing consecutive sampling. Data from the cholesterol and triglyceride total test were collected and analyzed with Mann-Whitney test. Additionally, HDL cholesterol and LDL was analyzed using T-Test. This Study showed that average total cholesterol from diabetics along with hypertension patients was in a range of 185.16 ± 36.59 and Diabetics patient without hypertension was 196.76 ± 44.62 (p = 0,503). Besides, the average rate of triglyceride level on diabetics patient with hypertension was 181.76 ±72.68 and diabetics patient along without hypertension was 180.28 ± 125.75 (p=0,367). The average rate of HDL cholesterol level on diabetics patient with hypertension was 39.12 ± 11.07 and diabetics patient along without hypertension was 42.37 ± 14.98 (p = 0,387). In addition, Average rate of LDL level on diabetics patient with hypertension was 112.57 ± 24.77 and diabetics patient along without hypertension was 118.33 ± 37.41 (p = 0,524). The average rate of uric acid on diabetics patient with hypertension was 6,120 ± 1,45 and diabetics patient along without hypertension was 5,768 ± 2,45 (p=0,200). As a conclusion, there is no difference with total cholesterol, triglyceride, HDL, LDL cholesterol, and uric acid significant from diabetics patient with or without hypertension in Ajibarang Hospital District Banyumas in 2016.

PERDAGANGAN ONLINE KURA-KURA DAN BULUS (REPTILIA: TESTUDINES) DI DAERAH ISTIMEWA YOGYAKARTA Lathifatul Faliha; Christian Manggala Yudha Pratama; Ainun Ni’matil Fitriyah; Rury Eprilurrahman
Scripta Biologica Vol 6, No 1 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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The Asian freshwater tortoise and fresh water turtle population is currently under threat from various types of human behavior (Van Dijk et al., 2000). In Indonesia, the turtle trade is increasingly prevalent because of the increase in turtle lovers in various regions and there are still people who consume turtles (soft shelled turtle). This study aims to find out the species and size variations of members Order Testudines traded in the Daerah Istimewa Yogyakarta. The method used in this study is online survey in social media such as Facebook, OLX Website, and Instagram to seller and reptile lovers (are traders too). The survey results in the online survey were 35 species from 9 families, which consists of 32 species (7 families) including turtle groups, and 3 species (2 families) including Soft Shelled Turtle. Two species is protected in Indonesia that is Orlitia borneensis and Carettochelys insculpta, then two species protect in Appendices I CITES that is Astrochelys radiata and Geochlemys hamiltonii, and the size variation the most common individuals are juvenile to adult individuals.

PENGARUH INOKULASI MIKORIZA VESIKULA ARBUSKULA (MVA) CAMPURAN TERHADAP KEMUNCULAN PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT (Solanum lycopersicum) Uswatun Hasanah; Purnomowati Purnomowati; Uki Dwiputranto
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Tomato has an important role to fullfil the nutrition of society. The most important problem in the cultivation of tomatoes is fusarium wilt caused by Fusarium oxysporum it will attacking the plants from nursery to adult. One of the alternative control is use the Vesicles Arbuscular Mycorrhizae (VAM). The success of VAM infection in plants is determined by the dose and the inoculation. The aim of this research is to determine the effect of dose and mixture VAM inoculation to against the emergence of fusarium wilt in tomato plants and to determine the dosage mixture VAM inoculation as the most effective way for controlling fusarium wilt in tomato plants. The method of this research used experimental with completely randomized design. The experimental treatment consists of two types of treatment that are combined with used 5 doses of VAM mixture (0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and used two ways of inoculation ( inoculation when the seed is planted and inoculation when transplanting the seeds). Each treatment was repeated 3 times and each test are three plants. The parameters was observed the incubation period of the disease and the intensity of fusarium wilt as the main parameter and the measurement of pH, temperature, humidity room, and the degree of infection as supporting parameters. The results of this research showed that the dosage and inoculation of VAM mixture is not able to reduce the emergence of fusarium wilt on tomatoes, but it was able to extend the incubation period of fusarium wilt on tomato plants a dose with 10 g /plant inoculated plants when the seeds are planted and inoculation when transplanting the seeds.

FITOREMEDIASI AIR LIMBAH PENCELUPAN BATIK PARAKANNYASAG, TASIKMALAYA MENGGUNAKAN KI APU (Pistia stratiotes L.) Tri Cahyanto; Tony Sudjarwo; Shinta Putri Larasati; Afriansyah Fadillah
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Batik home industry discharge untreated wastewater that can cause pollution to sewage water and its environment. Phytoremediation is the utilization of plants for environmental clean-up or reduces harmful contaminants including heavy metals such as chrome (Cr) of batik dyes. Water cabbage (Pistia stratiotes L.) locally named as Ki Apu is an aquatic plant known for its ability to reduce the Cr level in wastewater. This study aimed to determine the efficiency Ki Apu to reduce Cr level from batik dyeing wastewater from Parakannyasag, Tasikmalaya. This study was an experimental using Ki Apu from Indihiyang paddy field of Tasikmalaya. 12 individual of Ki Apu were grown on a 20 L water tank with different batik dyeing wastewater concentration of 0%, 25%, 50%, 75%, and 100%, each with five replicates. After 14 days the results showed the highest Cr reduction was measured at 77.5% occurred in 100% wastewater tank. Ki Apu leaf changed its leaf chlorophyll content with the highest estimate of 0.4 mg/L for chlorophyll-b and 1.3 mg/ L for the total chlorophyll; both occurred in 75% wastewater tank. The highest rate of phytoremediation indicated by BOD measure was at 33.4 mg/L/day occurred in 100% wastewater tank and by turbidity measure was at 23.3 mg/L/day occurred in 75% wastewater tank. Ki Apu is considered having a high phytoremediation effectivity to reduce Cr content in batik dyeing wastewater in Parakannyasag, Tasikmalaya.

DISTRIBUSI GEOGRAFIS TUNGAU PARASIT NYAMUK Aedes sp. DI DAERAH ENDEMIS DEMAM BERDARAH DENGUE DI KABUPATEN BANJARNEGARA Arthur Shepta Laksono; Bambang Heru Budianto; Endang Ariyani Setyowati
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Parasitic mites are known as the natural enemy of Aedes sp. that can potentially infect any life stages of the Aedes sp. The parasitic mites’ ability to infect Aedes sp. influence the distribution pattern of parasitic mites. The widespread distribution of Aedes sp. is expected to affect the distribution of parasitic mites. The aims of this study were to determine the taxonomic family of parasitic mites that infected Aedes sp. larvae and to determine the geographical distribution patterns of parasitic mites of Aedes sp. in the endemic area of Dengue Hemorrhagic Fever (DHF) in Banjarnegara District. This study used larvae of Aedes sp. taken from the endemic area of DHF in Banjarnegara District. The study sites were in the Parakancanggah Village, Kutabanjar Village, Krandegan Village, and Sokanandi Village. This study used survey method with purposive sampling technique. Variable observed in this research was the distribution pattern of the parasitic mite of Aedes sp. larvae. Parameter observed included the family and the individual number of parasitic mites on each mosquito larvae, the average value, and the variance. Data were analyzed using mean value and the variance so that the distribution pattern can be determined. The level of parasitic mites’ distribution was analyzed using K' index negative binomial distribution. Results showed that 30 individual of parasitic mite were found from 1429 samples of examined Aedes sp. larvae. The identification result were five families of parasitic mites: Pionidae, Histiostomatidae, Hydryphantidae, Hydrachnidae, and Arrenuridae. The distribution pattern of the parasitic mites was regular, and the highest value of K’ index negative binomial distribution was 1,3225, in the Krandegan Village.

INFESTASI CACING PADA SAPI POTONG PEMELIHARAAN INTENSIF DI KABUPATEN SLEMAN YOGYAKARTA Widodo Suwito
Scripta Biologica Vol 5, No 4 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Worm infestation in cow is economically disadvantageous such as inhibited the body growth, decrease milk production, and reduce the fertility. The aim of the current study was to determine type of infestation worm in cow which communal cage in Cageran, Taman Martani, Kalasan, Sleman and its relationship with type of feed and cleanliness on the floor cage. The samples were collected from faeces calves (n=25), cow (n=38), and bull (n=20). The samples were identified against worm eggs based on the morphology and size. The bivariate analysis chi square (χ2), odds ratio (OR), and relative risk (RR) were used to relationship worm infestation with feed type and cleanliness floor of the cage. Fasciola sp, Strongyl sp, and Ascaris sp are type of worm infestation in calves, cow, and bull. The Fasciola sp, is most founding in calves (n=10), cow (n=19), and bull (n=15), while Strongyl sp, and Ascaris sp are rarely. The feed of rice straw is association with fasciolosis in calves (χ2:6,25;OR:13,05;RR:2,33), cow (χ2:10,25;OR:8,25;RR:5,93), and bull (χ2:11,05;OR:5,13;RR:3,13). Association between cleanliness floor of the cage with infestation Fasciola sp, Strongyl sp, and Ascaris sp are (χ2:5,36;OR:12,31; RR: 3,28) in calves, cow (χ2:4,13;OR:3,31;RR:3,28), and bull (χ2:3,96;OR:1,17;RR:1,81). The study showed that Fasciola sp, is most widely worm infestation in calves, cow, and bull in communal cage in Cageran, Taman Martani, Kalasan. Feed of rice straw is potential causes of the Fasciola sp and cleanliness floor of the cage is more importance to prevent the worm infestation in calves, cow, and bull.

AKTIVITAS SUPEROKSIDA DISMUTASE TIKUS DIABETES YANG DIBERI EKSTRAK BATANG KAPULAGA DAN GLIBENKLAMID Gisti Rahmawati; Farida Nur Rachmawati; Hery Winarsi
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Superoxide Dismutase (SOD) is an antioxidant enzyme which reduce anion superoxide radicals as well as known caused of diabetes. There are many natural additive was believed having capacity to repaired an antioxidant celluler status. Cardamom’s stem were reported containing flavonoid and vitamin C which have been proven as in vitro antioxidant. There was no data showing its in vivo potency. This study aims to knoe the SOD activity of diabetes rats which were given cardamom stem extract (CSE) and glibenclamide. The research carried out with the use of experimentally Randomized Design Complete (RAL) by administering treatment on diabetes rat without CSE and glibenclamide as a control, consist of 100 mg/kg bodymass CSE and 2 mg/kg bodymass glibenclamide. The experiment consists of 3 treatments with 7 repetitions, blood sampling carried out experiments as much as 3 times with intervals of 7 days once. The data was analyzed using a variety of analysis (ANOVA). The result showed that the SOD activity increased from 4261 Unit/mg protein to 6604,668 Unit/mg protein (P<0.01) in diabetes rats treatment by CSE for 14 days.

KERAGAMAN GENETIK VARIETAS PADI japonica DAN indica BERDASARKAN MARKA DNA TERKAIT MUTU RASA Puji Lestari; Reflinur Reflinur; Dody Dwi Handoko; Mastur Mastur
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

PCR-based markers and evaluation of physicochemical properties should be addressed for the improvement of rice varieties with good eating dan eating quality (EQ). This study aimed to examine the genetic diversity of rice varieties based on DNA markers related to physicochemical properties determining EQ. A total of 46 rice varieties consisting of 22 japonica varieties and 24 indica varieties were examined using 43 PCR-based markers. The results showed that polymorphic information content (PIC) ranged from 0.04 to 0.38, in support of genetic diversity indices which ranged from 0.04 to 0.50 across total markers. Pairwise genetic similarity matrix ranged from 0.40 to 0.98 with the closest genetic distance was observed between two japonica varieties (Dongjin and Hwaseong) and the most distant one was between japonica and indica (Onnuri/Manmi with Cigeulis/Fatmawati). The unweighted neighbor-joining tree clustered the rice varieties into two major clades, indica and japonica, and subsequent subclades were differentiating according to the individual genetic background. The genetic diversity of rice from different subspecies and DNA markers for EQ can effectively be utilized for basic information and marker-assisted selection (MAS) for the development of improved varieties with good EQ in rice breeding program.

AUTHOR GUIDELINES and TABLE OF CONTENT Romanus Edy Prabowo
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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