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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 176 Documents

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KERAGAMAN GENETIK 24 VARIETAS PADI SAWAH DAN PADI GOGO (Oryza sativa L.) INDONESIA BERDASARKAN MARKA SSR Kristianto Nugroho; Slamet Slamet; Puji Lestari
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The use of molecular marker is an efficient approach to analyze the genetic diversity and it can be used widely in biological studies. The characterization of rice germplasms by using molecular markers technique is more precise because it is not influenced by environmental factors. The purpose of this study was to analyze the genetic diversity of 24 varieties of lowland and upland rice by using 15 SSR markers. The results showed as many as 86 alleles were detected in 24 rice varieties, with the average number of alleles per marker was 5.73 and the range of alleles per locus was 2-10. The average of major allele frequency was 43% with the lowest score was 26% on RM6997 and RM536 markers and the highest score was 65% on RM60 marker. A total of 14 SSR markers were able to discriminate heterozygous alleles within a range between 0.17 (RM105) to 1.00 (RM201, RM263, RM416, RM518 and RM223). The value of gene diversity ranged from 0.48 (RM60) to 0.81 (RM536) with an average of 0.70. The value of PIC (Polymorphic Information Content) ranged from 0.38 (RM105) to 0.78 (RM536) with an average of 0.65. The phylogenetic analysis showed that 24 rice varieties separate into two main clusters in the coefficient of 0.63. The first cluster consists of 12 lowland varieties and the second cluster consists of 12 upland varieties. The genetic diversity data in this study were expected could be a valuable information in the rice plant breeding activities in the future.

ANALISIS CEMARAN MIKROBA PADA DAGING AYAM BROILER DI KOTA MAKASSAR Sukmawati Sukmawati; Ratna Ratna; Ahmad Fahrizal
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Broiler chicken is one of the source of animal protein, which is favored by Indonesian society, because it has economic value and its availability is easy to be obtained both in traditional market and in modern market. This aim of this study is to determine the amount of microbial contamination in broiler chicken meat in the area of Makassar city. The method used in this research was descriptive method, that was test analysis of microbial contamination on broiler chicken sample which area in Makassar city. Of the five samples the largest value of colonies is 242 sample as much as 2412 cfu/g, while the smallest value of colonies is 247 sample of 435 cfu/g. According to the results of this study can be concluded that broiler chicken meat in the area of Makassar city is recommended for consumption.

IDENTIFIKASI BAKTERI PENGOKSIDASI BESI DAN SULFUR BERDASARKAN GEN 16S rRNA DARI LAHAN TAMBANG TIMAH DI BELITUNG Dhewanti Puspitasari; Hendro Pramono; Oedjijono Oedjijono
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Heavy metals contamination disturb balance and diversity of microorganism in soil. Microorganisms which can able to survive in those conditions are bacteria capable of oxidizing heavy metals. Identification based on 16S rRNA was used to determine characteristics and phylogenetic relationship of bacteria which can oxidize iron and sulphur in tin mining areas. The aim of this research was able to determine the bacterias characteristics isolated from tin mining areas and determine the phylogenetic relation of iron-sulphur oxidizing bacteria on tin mining soil in Belitung based on 16S rRNA sequences. This research was done using descriptive method, including isolation, morphological characterization, and identification based on 16S rRNA sequences. Morphology characterization includes colony and cell morphology through Gram staining. Molecular characterization includes amplification of 16S rRNA gene (Polymerase Chain Reaction/ PCR), electrophoresis amplicon and sequencing. Bacteria identification was done by comparing the 16S rRNA gene sequence in GenBank. The result showed three bacterias were identified by 16S rRNA have a similarity with Bacillus anthracis strain Ames, Bacillus cereus ATCC 14579, Staphylococcus sciuri subsp. Sciuri strains DSM 20345 and Micrococcus luteus NCTC 2665.

UPAYA MEMACU PERTUMBUHAN TUNAS MIKRO KENTANG KULTIVAR GRANOLA DENGAN JENIS DAN KONSENTRASI SITOKININ BERBEDA Adinda RN Pratama; Sugiyono Sugiyono; Lucky Prayoga; Ali Husni
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Micro-shoot growth is the first step in the potato microtuber formation. The main factors were known controlling microtuber formation including the media type, the concentration of sucrose, the type and concentration of plant growth regulators, temperature, and photoperiodicity. This research aimed to determine the influence of the t of cytokinin and its concentration on the micro-shoot formation of Granola cultivar of potato (Solanum tuberosum L.), as well as to determine the best cytokinin type and its concentration on micro-shoot formation of Granola cultivar potato (Solanum tuberosum L.). The research was experimental in a split-plot design. The main plot was the type of cytokinin (BAP and Kinetin), and the subplot was cytokinin concentration at five levels of concentrations, i.e., 0, 5, 10, 15 and 20 µM. This study replicated each treatment three times which resulted in obtaining 30 experimental units. The data obtained were then analyzed using an analysis of variance, followed by honest significant difference test at 95 and 99% levels of confidence. The results showed that the formation of micro-shoot of Granola cultivar of potato was controlled by the type and concentration of cytokinin used. The addition of 5‒15 μM of BAP was found to be the best treatments to stimulate micro-shoot formation of Granola cultivar of potato.

ANTIBACTERIAL CAPACITY OF Streptomyces ISOLATE FROM A MANGROVE PLANT RHIZOSPHERE Avicennia marina Rendy Setya Wardana; Dini Ryandini; Oedjijono Oedjijono
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

This research was conducted to obtain Streptomyces isolates from Avicennia marina rhizosphere capable of inhibiting E. coli and S. aureus growth, to investigate the capability and the characteristics of its antibacterial compound. This study completed the isolation by applying pour plate method on SCN agar medium. Antagonistic screening and selection processes were carried out by diffusion and dilution methods. Observation on the characteristic of the antibacterial compound applied was TLC method and MIC assay. This research confirmed the antibacterial compound capability by applying bioautography assay. Parameters measured consisted of inhibition zone diameter, Rf value on a bioautography plate, and the lowest concentration capable of inhibiting bacterial growth. Out of 16 isolates of Streptomyces obtained, Streptomyces 404 showed higher antagonistic activity than others. Inhibition zone diameter reached 20–25 mm in E. coli and S. aureus growth, respectively. TLC assay showed three spots in which two of them confirmed antibacterial activity in the bioautography assay that yielded Rf values of 0.47 for E. coli and 0.72 for S. aureus, while MIC assay showed that the lowest extract concentration inhibited bacterial growth was 20%.

HUBUNGAN SIKLUS MENSTRUASI DENGAN KADAR KALSIUM GINGIVAL CREVICULAR FLUID PENDERITA PERIODONTITIS KRONIS Amilia Ramadhani; Ziyada Salisa; Fani Tuti Handayani
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.827

Background: Periodontal disease is commonly caused by Porphyromonas gingivalis that released lipopolysaccharide endotoxin (LPS). The stimulation of pro-inflammatory cytokines secretion in periodontal tissue is not only derived from pathogenic bacteria. Various genetic conditions and systemic conditions are also being the trigger factor. One of systemic factor is sex hormones such as estrogen and progesterone. Both of these hormones fluctuate during the menstrual cycle. Purpose: The purpose was to determine the relationship of menstrual cycle based on ovarian cycle with calcium gingival crevicular fluid (GCF) levels in chronic periodontitis patient. Methode: This research was analytic observation. The samples in this reasearch were 9 women who aged 17-45 years with a diagnosis of chronic periodontitis and had a normal menstrual cycle (21-35 days). All subjects were calculated menstrual cycle and determined the phase of follicular, ovulation, and luteal. Clinical examination and GCF sampling were performed at each phase. Measurement of calcium GCF level was done using spectrophotometer. Result: During follicular phase of menstruation cycle, the periodontal index arises until 1,62, meanwhile the highest calcium concentration occurred in ovulation phase of menstruation cycle. Linier regression correlation test showed no significant correlation between periodontal index, calcium concentration and menstruation cycle. Conclusion: Fluctuation in women’s sexuality hormones during menstruation cycle do not related to alveolar bone damage and GCF’s calcium concentration.

MENGISI KESENJANGAN ANTARA JUMLAH HASIL PENELITIAN MAHASISWA DALAM BIDANG BIOLOGI DAN KETERSEDIAAN MEDIA PUBLIKASINYA Romanus Edy Prabowo
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Berkala ilmiah Scripta Biologica ini dibuat untuk mengisi celah besar kesenjangan antara jumlah hasil penelitian mahasiswa dalam bidang biologi dan ketersediaan media publikasinya. Scripta Biologica tersedia secara online dalam 4 nomer terbitan per volume per tahun, kualitas artikel ilmiahnya tetap dipertahankan dengan kombinasi editorial-review dan peer-review. Scripta Biologica menerapkan lisensi Creative Commons Attribution BY-SA, di bawah lisensi ini hak cipta atas artikel ilmiah yang dipublikasikan tetap ada pada penulis. Penulis memberikan lisensi kepada Scripta Biologica untuk mempublikasikan artikel ilmiahnya dan menyatakan Scripta Biologica sebagai penerbit pertama, sedangkan pembaca dapat menggunakannya secara bebas; mengunduh, mencetak, menggunakan, mengubah, menyebarkan, dan atau menyalin sebagian atau seluruh artikel ilmiah dalam berkala ini, selama identitas penulis, detil sitasi, dan berkala serta penerbit tetap disertakan dan dinyatakan secara jelas. Semoga berkala ilmiah online Scripta Biologica bisa memberikan kontribusi dalam usaha bersama insan akademik untuk meningkatkan akses pada hasil-hasil penelitian, utamanya penelitian mahasiswa dalam bidang biologi, sehingga pengetahuan serta manfaatnya dapat digunakan oleh masyarakat secara lebih luas. Publikasi perdana Scripta Biologica volume 1 nomer 1 ini adalah langkah awal untuk secara berkelanjutan membuat lebih banyak hasil penelitian mahasiswa dalam bidang biologi menjadi terdiseminasi, terdokumentasi, dan tersertifikasi.

CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM Solanum tuberosum L. var. Granola POTATO Yunior William Susanto; Hermin Pancasakti Kusumaningrum; Siti Nur Jannah; Sri Rustini
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Potato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe.

KONDISI EKOSISTEM TERUMBU KARANG DI LOKASI TIATIKI DAN NON-TIAITIKI, KAMPUNG TABLANUSU DISTRIK DEPAPRE KABUPATEN JAYAPURA Yunus Pajanjan Paulangan
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.688

Depapre Bay has large potential fisheries resources and very diverse that are still managed by the Tiaitiki system. Ironically, destructive fishing activities still occur. The coral ecosystems in Depapre Bay are still relatively under-studied mainly related to sustainable management. Condition of coral reef and reef fishes are the base aspect in management approach. The purpose of this research is to analyze coral reef and reef fishes condition of Tiatiki area and outside of Tiaitiki. The method used in this research is Point Intercept Transect (PIT) to identify coral condition, and Underwater Visual Cencus Method (UVC) for reef fishes observation. The results showed that lifeform conditions at 3-5 m of Tiaitiki area were "40,67% was moderate, dominated by Coral Branching, Flassy Micro Alga dan Coral Foliose; and at 10-13 m of Tiaitiki area were 50,00% was “good”. Lifeform conditions at 3-5 m of non-Tiaitiki area were "48,67% was moderate, dominated by Coral Branching, Coral Massive dan Flassy Micro Alga; and at 10-13 m of non-Tiaitiki area were 60,67% was “good”, dominated by Coral Branching, Acropora Branching, Acropora Tabulate, Acropora Encrusting dan Coral Foliose. In addition, at the all areas found rubbles is quite high at about 8-16,67%. Abundance of reef fishes in both areas is relatively the same, where in Tiaitiki area that are 372 individual at 3-5 m of depth and 114 individual at 10-13 m of depth, and Non-Tiaitiki area that are 262 individual at 3-5 m and 215 individual at 10-13 m.

KELIMPAHAN CHRYSOPHYTA PADA MEDIA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI DENGAN PENAMBAHAN TEPUNG KULIT UBI KAYU DAN PROBIOTIK Retno Ambarwati; Endang Widyastuti; Dwi Sunu Widyartini
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Chrysophyta is also known as golden-yellow algae because of the yellow dominant pigment of carotene and xanthophyll. This study aimed to determine species richness and abundance of Chrysophyta in the Tilapia culture media which was fed with the addition of cassava peel flour and probiotic. The method applied in this study was the experimental method with 4 treatments, i.e.: the use of fermented feed with the addition of cassava peel flour (0%, 25%, 50%, and 75%) and probiotic in Tilapia culture media. Each treatment was replicated 4 times. Sampling was carried out 6 times at intervals of 2 weeks. The observed parameters were the main parameters, i.e.: the number of Chrysophyta species and individuals; and supporting parameters, i.e.: water temperature, pH, TDS, TSS, NO3, NO2, BOD5, DO, and total of phosphate. Species richness and abundance of Chrysophyta data were analyzed using cluster analysis based on Bray-Curtis similarity coefficient. The analysis continued with Similarity Percentages (SIMPER) analysis to determine the contribution of species to the similarity index in each group or to dissimilarity index amongst groups. The results showed that the species richness found in the Tilapia media culture which was fed with the addition of cassava peel flour and probiotic consist of 20 species belonging to a class namely Bacillariophyceae. Abundance of Chrysophyta obtained was ranged from 5.160-13.292 individuals/liter. The cluster analysis showed that Chrysophyta amongst treatments have a quite high similarity level (> 50%) was ranged between 65.56% -83.99%. Contributions of species which contribute the highest similarity index were Diatoma vulgare (49.80%), Navicula brachysira (70.50%) and Navicula platystoma (82%).

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