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INDONESIA
Jurnal Biotek
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23549106     EISSN : 25811827     DOI : -
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education
Jurnal Biotek is an academic journal published by Alauddin Press Makassar in collaboration with Department of Biology Education in Universitas Islam Negeri Alauddin Makassar. The journal accepts scholarly papers regarding of biology science, biology education, Islamic perspective of biology science, etc. Publication time is June and December.
Arjuna Subject : -
Articles 13 Documents
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Search results for , issue "Vol 12 No 2 (2024): DESEMBER" : 13 Documents clear
Analisis Kualitas Ragi Roti Sourdough pada Berbagai Jenis Tepung dengan Penambahan Ekstrak Tomat (Solanum lycopersicium) Dewi, Putri Rosfiana; Sari, Laela Dwi Kartiko; Windayanti , Aridha Elfarikha; Cica, Siska Aliatuliyah; Nikmah, Alvi Lailatun; Isnawati , Isnawati
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.34387

Abstract

Sourdough yeast is a natural yeast for making bread that aims to slow down the decay process. The fermentation process can be assisted by using tomato extract. This study aims to obtain the best quality yeast from various types of flour with the addition of tomato extract (Solanum lycopersicum). This experimental study was designed with independent variables in the form of flour types (wheat, wheat, glutinous rice), control variables in the form of tomato extract and the ratio of material composition (1:1), and response variables in the form of yeast quality (color, odor, shape, presence of foreign objects, and yeast activity). Data were analyzed descriptively and compared with yeast quality standards based on SNI (Indonesian National Standard). The results showed that wheat flour produced the best sourdough yeast. The results showed that wheat flour met the yeast requirements based on SNI 01-2982-1992, had a yellowish white color with a normal odor, good acidity, in the form of coarse powder with no foreign objects and good yeast activity. Unlike wheat flour, which has a brownish white color, a very sour normal odor, a coarse powdery texture, and no foreign objects, but its activity is not good so it does not meet the SNI 01-2982-1992 standard. Glutinous rice flour is white, has a slightly sour normal odor, a fine powdery form, and no foreign objects, its activity is very good, but does not meet the SNI 01-2982-1992 standard because of its color and texture.
Karakteristik dan Aktivitas Antioksidan Tepung Kulit Buah Naga (Hylocerus polyrhizus) pada Brownies Pukis Kulit Buah Naga (Bruga) Lestari, Putri Kurnia; Nazihah, Fathimah Qonita; Nadhilah, Aulia Shafra; Safitri, Qorina Amalia; Isnawati, Isnawati
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.38100

Abstract

Dragon fruit is a fruit that contains many benefits inside it. One part of the dragon fruit that has high nutritional value is the skin. The skin can be processed into products made into flour which can be used as an ingredient in cakes. The utilization of this dragon fruit skin to cakes can also add nutritional benefits to cakes itself. Dragon fruit skin flour itself has a rich content of antioxidants. These antioxidants have the benefit of protecting the body from various kinds of disease and can protect the body against the effects of free radicals. Apart from that, the utilization of this dragon fruit skin can be used to reduce dragon fruit waste which often pollutes the surrounding environment. This dragon fruit waste is still minimally utilized in the wider community so that accumulation often occurs. Therefore, holding this research can add efficacy and nutritional value to cakes and reduce the amount of dragon fruit peel waste in the surrounding environment to create a clean and eco-friendly environment. The purpose of this study is to analyze the characteristics and also the antioxidant activity contained in dragon fruit peel flour which would be made in the form of pukis brownies. These pukis brownies were divided into 3 concentrations, contain with 100% wheat flour, 50% dragon fruit peel flour, and 100% dragon fruit peel flour by analyzing the starch content and the influence of the quality of the pukis brownies in terms of shape, color, taste, smell and texture.
Analisis Sikap Peduli Lingkungan Siswa Berdasarkan Gender Hafiza, Auramia; Ferry, Dharma
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.45929

Abstract

Environmental concern is crucial for understanding individual and societal behaviors related to environmental preservation. Environmental concern is a key factor influencing pro-environmental behaviors, such as sustainable resource usage and support for environmental policies. This study aimed to understand the differences in environmental concerns between male and female students at MAN 1 Kabupaten Kerinci. The research method was a survey with a quantitative approach involving 122 students from grades XI and XII. The research instrument consisted of a questionnaire on environmental concerns comprising 22 statements. The results of the analysis indicate significant differences in environmental concerns between genders. The proportion of female students concerned about the environment (86.9%) is substantially higher than that of male students (13.1%). The majority of respondents demonstrated high or very high levels of environmental concern. The data also reveal disparities in overall scores, with female students tending to score higher. Further analysis of attitude indicators shows that female students consistently exhibit higher levels of environmental concern than male students across all indicators. This study provides in-depth insights into the differences in environmental concerns based on gender at MAN 1 Kabupaten Kerinci.
Meningkatkan Hasil Belajar Peserta Didik dengan Model Pembelajaran Kooperatif Berbantuan Flashcard Materi Klasifikasi Makhluk Hidup Salbiah, Salbiah; Titin, Titin; Wahyuni, Eko Sri; Ningsih, Kurnia; Yuniarti, Anisyah
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.48340

Abstract

This study aimed to improve the learning outcomes of seventh-grade students in class VII A at SMPN 22 Pontianak on living organisms' classification through a cooperative learning model assisted by flashcards. The Classroom Action Research (CAR) model has two cycles, each including four stages: planning, implementation, observation, and reflection. Data collection methods involved the learning outcome tests, observation sheets, and documentation. This research focused on analyzing changes in students' understanding of active and interactive learning strategies using flashcards. The results showed a significant improvement in student learning outcomes; the mastery level was 81.25% in the first cycle, which increased to 87.50% in the second cycle. This increase indicates that the cooperative learning model assisted by flashcards not only enhances student engagement in the teaching-learning process but also effectively strengthens their understanding of the material on the classification of living organisms. The findings suggest that integrating cooperative methods with visual aids, like flashcards, can be an effective pedagogical strategy to enhance the quality of education in schools.
Desain Primer In Silico Untuk Analisis Expresi Gen Osteopontin (OPN) Sebagai Penanda Kesuburan Pada Kambing Jantan (Capra hircus) Sukoco, Hendro; Suriansyah, Suriansyah; Salmin, Salmin; Cahyani, Annisa Putri; Andanawari , Suci; Akbarrizki , Muzizat; Siswanto, Ferbian Milas
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.49469

Abstract

This study aimed to design a pair of primers for analyzing OPN gene expression as a fertility marker in male goats (Capra hircus). The research method employed was an in silico molecular study based on bioinformatics. Nucleotide data were retrieved from the NCBI database using the code NM_001285667.1. The data obtained were processed using Primer-Blast on the NCBI site to identify candidate primers. These candidate primers were subsequently analyzed for secondary structure and primer dimer formation using the Sigma-Aldrich OligoEvaluator.The designed primers were further analyzed for their specifications and sequence similarity with the nucleotide database available on the NCBI site using Primer-Blast and Nucleotide-Blast. The results revealed one pair of primers with ideal criteria: the forward primer 5'-ACCCTCCCGAGTAAGTCCAA-3' and the reverse primer 5'-AGTCCTCCTCTGTGGCATCT-3', capable of amplifying the OPN gene region with a product length of 325 bp. In silico performance tests also demonstrated that the primers could specifically detect the OPN gene in Capra hircus. The primer design for detecting OPN expression is expected to aid in predicting fertility levels in male goats (Capra hircus), thereby enhancing the reproductive efficiency of these livestock.
Etnobotani Tumbuhan Obat Tradisional untuk Mengatasi Hipertensi oleh Etnik Melayu Mulia Kerta Ketapang Nurfitri, Hany; Syamswisna, Syamswisna; Titin, Titin
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.49862

Abstract

The rich flora of Indonesia plays a significant role in the lives of its people, particularly in health. The Mulia Kerta Ketapang Melayu ethnic group is one community that utilizes herbs for traditional medicine, especially for treating hypertension, a common condition in society. Knowledge of plant use for traditional hypertension medicine is passed down orally from generation to generation, resulting in a lack of proper documentation. This research aimed to document the plant species, identities, parts used, and procedures for utilizing plants as hypertension medicine by the Mulia Kerta Ketapang Melayu ethnic group. This study is a qualitative descriptive research employing triangulation methods (interviews, observation, and documentation) and plant identification as the primary data collection tools. The results of the study identified 33 plant species used as traditional hypertension medicine. Plants from the Rutaceae and Lamiaceae families were the most commonly utilized, each accounting for 9% of the species. Leaves were the most frequently used plant part, constituting 43.2%, while the boiling method was the most commonly employed preparation technique, used in 58% of cases. The findings of this research can serve as a reference for further studies and as a basis for comparing the similarities or connections between the Mulia Kerta Ketapang Melayu ethnic group and other ethnic groups in their use of traditional medicinal plants.
Uji Antibakteri Ekstrak Batang Bajakah Berduri (Caesalpinia sumatrana Roxb.) terhadap Salmonella typhi ATCC 19430 Mufidah, Annisa Nabilah; Yeni, Laili Fitri; Wahyuni, Eko Sri
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.50061

Abstract

Thorny bajakah (Caesalpinia sumatrana Roxb.) is a plant from the Fabaceae family known to contain secondary metabolites with antimicrobial activities. This study aimed to examine the antibacterial activity and the effect of variation in the concentration of thorny bajakah stem extract against Salmonella typhi ATCC 19430. The samples included thorny bajakah stem extracts at concentrations of 12.5%, 25%, 50%, and 100%, along with a negative control (10% DMSO) and a positive control (0.001% chloramphenicol). Extraction was done using maceration with 96% ethanol as the solvent, while an antibacterial test was carried out using the disc diffusion method. Antibacterial tests showed the inhibition zones by each treatment of 12.5%, 25%, 50%, and 100% were 3.05 mm, 3.89mm, 3.69mm, and 4.56mm. The 100% concentration extract has the highest average diameter of the inhibition zone, even though it is considered very weak according to the Morales standard (<6mm). The antimicrobial activity is affected by the composition of secondary metabolites in the extract present as alkaloids and terpenoids, which are known to have antimicrobial activity. These results provide a basis for further research to develop the potential of thorny bajakah as a plant-derived antibacterial agent.
The Preliminary Study of Electrigens Potential from Wastewater as The Manifestation Of "Khalifah Fil Tadbir" Conception Hafsan, Hafsan; Asaad, Andi Indra Jaya; Arsyad, Muh Ali; Ahmad, Arifuddin
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.50112

Abstract

This study reveals that the super-intensive shrimp farming waste from Punaga can be used as a source of electricity through a bio-electrogenic process based on indigenous potential bacteria from the waste. The results show that four types of potential bacteria isolated from the super-intensive shrimp farming waste can each produce a significant average power density. Bacteria isolate D (Bacillus sp) showed the highest electrogenic potential with an average power density of 38.55±0.87 mW/m², followed by bacteria isolate B (Pseudomonas sp) with an average power density of 34.72±2.80 mW/m², bacteria isolate C (Bacillus sp) with an average power density of 29.90±5.26 mW/m², and bacteria isolate A (Bacillus sp) with an average power density of 25.90±1.69 mW/m². This study shows that the super-intensive shrimp farming waste from Punaga can be utilized as a source of electricity through a bio-electrogenic process using certain potential bacteria, such as Bacillus sp and Pseudomonas sp isolates. The development of waste treatment technology as a source of electricity through the bio-electrogenic process can provide a significant contribution to addressing global energy and environmental issues. This can be considered an implementation of the concept of "khalifah fil tadbir", in which humans act as stewards of the earth, responsible for using natural resources wisely and being accountable for the resulting environmental impacts.
Ekstrak Buah Lerak (Sapindus rarak) sebagai Sabun Antibakteri yang Ramah Lingkungan Novitarini, Novitarini; Kresnapati, I Nyoman Bagus Aji; Akmi, Alfarizi Muzaifa
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.51245

Abstract

Synthetic surfactants contribute to environmental pollution and can cause skin irritation, highlighting the need for natural biosurfactants. Lerak fruit (Sapindus rarak) shows promise as a biosurfactant due to its 28% saponin content and potential antibacterial activity. This study aimed to evaluate the antibacterial activity of lerak fruit extract using three parameters: inhibition zone, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) against Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative). The research utilized a Posttest Only Control Group Design to test the antibacterial activity of lerak fruit extract against skin-surface normal flora, specifically Staphylococcus aureus and Pseudomonas aeruginosa. All antibacterial activity results were analyzed using One-Way ANOVA in SPSS IBM version 22. The study found the MIC to be 6.25% for Gram-positive bacteria and 1.56% for Gram-negative bacteria. The MBC was determined to be 25% for Gram-positive bacteria and 50% for Gram-negative bacteria. At a 50% concentration, the inhibition zones against Gram-positive and Gram-negative bacteria were 21.53 mm and 22.13 mm, respectively. These results demonstrate that lerak fruit extract possesses strong antibacterial activity against both Gram-positive and Gram-negative bacteria. This research provides a foundation for developing environmentally friendly antibacterial soap formulations using lerak fruit extract.
Pengembangan Buku Petunjuk Praktikum Lapangan Berbasis Jigsaw Sebagai Sumber Belajar Identifikasi Vertebrata Nasrianty, Nasrianty; Pratomo, Ryan Humardani Syam
Jurnal Biotek Vol 12 No 2 (2024): DESEMBER
Publisher : Department of Biology Education of Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jb.v12i2.51562

Abstract

This research aimed to evaluate the validity and feasibility of the Jigsaw-based field practicum guidebook as a learning resource for vertebrate identification. The development of the guidebook followed the Borg and Gall model for research-based product development. The research stages included a preliminary study and a field trial process. Based on assessments from two validators, the guidebook received an average score of 4.68, which falls within the "very good" category. The researcher conducted a limited trial with 10 students who varied in ability. The results of the limited trials showed average percentage scores of 84% (high ability), 79% (medium ability), and 77% (low ability). For the field trial, a one-group pre-test and post-test design was applied. Data analysis was performed using SPSS 23 for Windows, employing the paired t-test formula. A significance value of 0.000 was obtained from the field trial analysis, indicating a value lower than 0.05. Therefore, it can be concluded that there is a significant difference between students' pre-test and post-test scores. The results showed that the field practicum guidebook developed by the researcher is suitable for learning.

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