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Contact Name
Ari Khusuma
Contact Email
khusumaari@gmail.com
Phone
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Journal Mail Official
khusumaari@gmail.com
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Location
Kota mataram,
Nusa tenggara barat
INDONESIA
Jurnal Analis Medika Biosains (JAMBS)
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 319 Documents
Bacterial Contamination Analysis Based On Total Plate Count From Various Uses Of Compact Powder Putri, Ni Putu Sintyani; Kristinawati, Erna; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.470

Abstract

The repeated and alternating use of compact powder can increase the risk of bacterical contamination. This contamination can be harmful to the skin health of the users, especially if the compact powder used has been contamination with bacteria exceeding the standard limit set by BPOM, which is 5 × 10³ colonies/g or colonies/mL. Therefore, an analysis of bacterial contamination in the repeatedly and alternately used sompact powder from various uses is necessary using the Total Plate Cound (TPC) method to ensure the suitability and safety of the compact powder. The objective is to determine the level of bacterial contamination based on the Total Plate Cound (TPC) on compact powder used by makeup artists, tester from cosmetic stores, personal use dan new product. The samples of conpact powder analyzed amounted to 24 samples, using purposive sampling technique and the data was analyzed descriptively. The result obtained from the calculation of the average number of bacterical colonies in personal use compact powder, new product and tester from each cosmetic store are < 3 x 10¹ CFU/g, while the average for compact powders sourced from makeup artists is 1.66 x 10^5 CFU/g. Compact powder derived from personal use, new products, and those used as testers by cosmetic stores show ALT values that still fall within the category of meeting the standards set by BPOM. Meanwhile, compact powder derived from makeup artists shows ALT values that exceed the standards set by BPOM.  
Comparison of Neutrophil-to-Lymphocyte Ratio (NLR) in Gram-Positive and Gram-Negative Bacteremia from Blood Specimens Surahmat, Erik; Suhartini, Anita
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.533

Abstract

Bacteremia remains a significant cause of morbidity and mortality worldwide, with both Gram-positive and Gram-negative bacteria contributing to the disease burden. The neutrophil-to-lymphocyte ratio (NLR) has emerged as a simple, cost-effective biomarker for evaluating inflammation and infection severity. This study aimed to compare the NLR values between patients with Gram-positive and Gram-negative bacteremia confirmed by blood culture. An analytical observational cross-sectional study was conducted at Bayu Asih Purwakarta Hospital from January to July 2025. Data from patients with positive blood cultures and complete hematological profiles were included and analyzed using the Mann-Whitney U test. A total of 84 cases were studied, consisting of 42 Gram-positive and 42 Gram-negative bacteremia. The results showed a statistically significant difference in NLR values between the two groups (p = 0.038. These findings support the utility of NLR as a useful biomarker in differentiating types of bacterial infections in bacteremia cases. In conclusion, NLR may aid in early diagnosis and clinical decision-making for bacteremia management.
Detection of Nicotine in Urine of Passive Smokers Exposed to Cigarette Smoke From Smoking and Non-Smoking Families Aolani, Almira Zaida; Fihiruddin, Fihiruddin; Urip, Urip; Tatontos, Erlin Yustin
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.459

Abstract

Passive smokers are vulnerable to nicotine exposure, which can increase the risk of health problems. Nicotine is a chemical compound in tobacco that causes dependence and can be detected in urine as metabolites like cotinine. This study aimed to detect nicotine in the urine of passive smokers exposed to cigarette smoke from smoking and non-smoking families in Genggelang Village. An analytical observational method with a cross-sectional design was used. The sample consisted of 24 respondents selected by purposive sampling, divided into two groups. Urine samples were tested using a nicotine test strip and analyzed descriptively. The results showed that among 12 passive smokers from smoking families, one respondent tested positive for nicotine. Meanwhile, all 12 respondents from non-smoking families tested negative. These findings indicate that passive smokers living with smoking families are still at risk of nicotine exposure through second hand smoke in the household environment.
Differences In Hemoglobin Levels In Infected Patients Salmonella Typhi With Salmonella Paratyphi Based On Antibody Titer Hijriyani, Laelin; Fihiruddin, Fihiruddin; Pauzi, Iswari; Resnhaleksmana, Ersandhi
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

Salmonella typhi and Salmonella paratyphi cause the disease known as typhus abdominalis. The pathogenicity that distinguishes Salmonella typhi and Salmonella paratyphi comes from the virulent part of the bacterial capsule. Virulence factors are found on the surface of Salmonella typhi that are not found in Salmonella paratyphi. The toxic effect of Salmonella that suppresses the bone marrow and the presence of bleeding in the intestine can cause anemia, so a supporting examination is needed, one of which is the examination of Hemoglobin levels. The aimt of this study is knowing the difference of hemoglobin levels in patients infected with Salmonella typhi and Salmonella paratyphi based on antibody titer against H antigens: This study used Analytical Observational method with cross-sectional approach. Data obtained from typhoid fever patients from the examination of hemoglobin levels at Patut Patuh Patju Hospital as many as 96 people using secondary data.  "The mean hemoglobin level for Salmonella typhi, Salmonella paratyphi A, and Salmonella paratyphi B was 9.7 g/dL, 12.0 g/dL, and 12.6 g/dL, respectively. At antibody titers of 1/160 and 1/320, the mean hemoglobin levels for S. typhi were 10.5 g/dL and 9.2 g/dL; for S. paratyphi A, 12.0 g/dL and 11.8 g/dL; and for S. paratyphi B, 12.8 g/dL and 11.6 g/dL. According to the ANOVA test, there is a significant difference in the average hemoglobin levels among patients infected with S. typhi compared to those with S. paratyphi based on these antibody titers (< 0,001)
Differences in Random Blood Glucose Levels on Acceptors of the 1-Month Injection Contraceptive Program and the 3-Month Injection Contraceptive Program in the Working Area of the Masbagik Community Health Center Diarti, Maruni Wiwin; Mulyadi, Faiza Waziran; Pauzi, Iswari; Urip, Urip; Jiwintarum, Yunan
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

Glucose is one of the products of carbohydrate metabolism in the body that functions as the main source of energy for the body, controlled by insulin. This increase in blood sugar is caused by the inefficient work of insulin, which is caused by the presence of injectable contraceptives. The progesterone hormone contained in injectable contraceptives has anti-insulin properties that cause cells to become less sensitive. Reduced insulin sensitivity can lead to insulin resistance in the body, which can affect glucose metabolism and result in elevated blood sugar levels. The objective of this study is to determine the difference in blood glucose levels among participants in the 1-month injection contraceptive program and the 3-month injection contraceptive program in the Masbagik Health Center service area. The sample in this study consisted of participants in the 1-month and 3-month injection contraceptive programs, with a total of 26 respondents (13 for each group). The data collected were glucose levels measured using the POCT (point-of-care testing) method. The data were analyzed using the Mann-Whitney statistical test. The results showed that the mean glucose level in 1-month contraceptive injection program participants was 108.8 mg/dL, while in 3-month program participants it was 142.9 mg/dL. The Mann-Whitney test results showed no significant difference between the blood glucose levels of 1-month contraceptive injection users and 3-month contraceptive injection users, with a value of 0.068 (p > 0.05). The conclusion of this study is that there is no significant difference in blood glucose levels between 1-month and 3-month contraceptive injection users. However, mathematically, there is an increase in the average values.
Analysis Of The Effect Of Hemodialysis Frequency On Hemoglobin, Ureum, Creatinine And Albumin Levels In Chronic Kidney Failure Patients At Praya Hospital Jiwintarum, Yunan; Anindita, Kurnia; Tatontos, Erlin Yustin; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

Chronic Kidney Disiase (CKD) is a progressive loss of kidney function characterized by the inability of the kidneys to excrete waste and maintain metabolic balance. When kidney function declines, erythropoietin production decreases, resulting in anemia. other complication, such a hypoalbuminemia, are also common. Urea and creatinine levels are vital parameters of assessing kidney function. Hemodialysis is a common therapy for CKD patients; therefore, evaluating laboratory parameters such as haemoglobin, urea, creatinine, and albumin levels according to the frequency of hemodialysis is essential to determine treatment effectiveness. This analytical observational study with a cross-sectional design evaluated the effectiveness of bi-weekly hemodialysis among 32 chronic kidney disease patients at Praya General Hospital in May 2025. The results indicated that while routine hemodialysis significantly reduced urea levels from 137.9 mg/dL to 17.6 mg/dL and creatinine from 10.7 mg/dL to 2.5 mg/dL, these metabolic waste parameters tended to increase slightly after six months. In contrast, hemoglobin levels remained relatively stagnant at approximately 8.2–8.3 g/dL, signifying persistent moderate anemia despite regular treatment. Notably, albumin levels showed a steady and gradual improvement over the six-month period, rising from 3.4 g/dL to 4.0 g/dL, which suggests an enhanced protein status in patients adhering to the prescribed hemodialysis frequency.
Relationship of Leukocyte and Platelet Counts with NS-1 Antigen Examination Results in Suspected Dengue Fever Patients Utami, Sherly Suryandari; Priyatno, Djoko
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

Dengue fever is still a global health problem, with high mortality rates due to dengue fever. Leukopenia and thrombocytopenia often occurs in dengue fever patients. Early detection of dengue virus infection through dengue NS-1 is needed to avoid delays in diagnosis where NS-1 has high sensitivity in detecting dengue virus infection in the early phase. A decrease in the number of leukocytes and platelets is a finding that can provide an indication of the severity of the disease and assist in the diagnosis of DHF. The aim of this research was to establish the relationship between total platelet and leukocyte counts and NS-1 antigen assay findings in individuals with suspected hemolytic disease of the liver (HDL). The research was quantitative, with an observational design and a cross-sectional approach. The study population consisted of 91 patients with suspected HDL at the Mayong II Jepara Health Center between January and December 2024. Total sampling was used. The findings indicated that males were the predominant group of patients with suspected HDL, and that 62% of them were in the 0-5 year age group. The average number of leukocytes in patients was 12,457 ± 3,471 cells/mm3 of blood with the lowest leukocyte count of 3,400 cells/mm3 and the highest 20,500 cells/mm3. The average number of platelets was 162,626 ± 72,930 cells/mm3 of blood with the lowest platelet count of 54,000 cells/mm3 and the highest 385,000 cells/mm3. The results of the NS-1 examination of suspected DHF patients were 69% positive and 31% negative. There was no relationship between the number of leukocytes and the NS-1 antigen examination (p value 0.220>0.05) but there was a relationship between the number of platelets and the NS-1 antigen examination (p value 0.00<0.05) in suspected DHF patients at Mayong II Jepara Health Center.
Overview of Platelet Counts Before and After Platelet-Rich Plasma (PRP) Preparation Yuliandari, Aisyara; Mahrani, Izah; Zafrida, Siska; Hartini , Hartini; Fauzan, Fauzan
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

Platelet-Rich Plasma (PRP) is widely utilized in various clinical settings for tissue repair, particularly in skin rejuvenation, scar management, and wound healing. This study aimed to evaluate the profile of platelet counts both prior to and following the PRP preparation process. Whole blood samples were collected from eligible female participants and platelet levels analyzed using a hematology analyzer. The findings revealed a four-fold rise in the mean platelet count, increasing from a baseline of 190.6×103 cells/µL to 781.1×103 cells/μL after preparation. The study concluded that baseline platelet levels in whole blood are directly proportional to the final platelet concentration in PRP.
Isolation and Screening of Proteolytic Bacteria Isolated from the Digestive Tract of Pangasius Catfish Patin (Pangasius hypophthalmus) Zafrida, Siska; Lasmini, Titi; Hartini, Hartini; Aruan, Anggi Agustina
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

The demand for protease enzymes in Indonesia has exhibited a significant upward trend; however, domestic large-scale production capacity remains limited. To mitigate dependency on imports, strategic efforts to develop indigenous protease production are essential. This study aims to identify and isolate protease-producing bacteria from the digestive tract of catfish (Pangasius hypophthalmus). This research employed an observational method with a cross-sectional design. Samples were obtained from the stomach of the catfish and subsequently inoculated into Brain Heart Infusion (BHI) media. Bacterial isolation was performed using Blood Agar Plate (BAP) and MacConkey Agar. The proteolytic activity of the resulting isolates was evaluated on Skim Milk Agar (SMA), followed by macroscopic and microscopic characterization. The study yielded three pure isolates; however, screening for proteolytic activity revealed that only one isolate, designated as PHS1, exhibited protease production. The proteolytic activity was characterized by the formation of a 2 mm clear zone (lysis zone) with a proteolytic index of 3. Microscopic analysis identified the isolate as a Gram-positive, spore-forming bacterium.

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