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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
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Penyimpanan dan Penambahan Kurma Ajwa Terhadap Aktivitas Antioksidan Infused Water Stroberi dan Wortel Dewi, Luh Putu Rahayu Candra; Lestari, Indah; Rahayuningsih, Christ Kartika; Diarti, Maruni Wiwin
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.274

Abstract

Radikal bebas merupakan senyawa yang sangat reaktif dan dapat merusak molekul pembentuk sel dalam tubuh sehingga terjadi kemunduran fungsi organ dan memicu timbulnya penyakit degeneratif. Infused water merupakan alternatif dalam proses detoksifikasi radikal bebas sekaligus pemenuhan kebutuhan cairan tubuh harian. Stroberi banyak dijadikan sebagai bahan utama infused water karena kandungan vitamin C yang tinggi dan dikombinasikan dengan wortel yang kaya akan beta karoten sedangkan kurma ajwa dijadikan sebagai penambah cita rasa. Kandungan antioksidan total dalam infused water mudah mengalami degradasi fisik maupun kimia selama proses penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh penyimpanan dan penambahan kurma ajwa terhadap aktivitas antioksidan infused water stroberi dan wortel. Jenis penelitian yang digunakan adalah eksperimental dengan rancangan post test only control group design yang dilaksanakan di Laboratorium Proteomik Institute Tropical Disease Universitas Airlangga dan Laboratorium Kimia AMAMI Jurusan Analis Kesehatan Poltekkes Kemenkes Surabaya pada bulan Oktober 2019 sampai Juni 2020. Penelitian ini terdiri dari dua kelompok infused water dengan dan tanpa penambahan kurma ajwa yang disimpan selama 0 jam sebagai kontrol, 2 jam dengan pengisian ulang pertama dan 4 jam dengan pengisian ulang kedua pada suhu ruang (±28oC) dan suhu refrigerator (±10oC). Masing - masing sampel diuji aktivitas antioksidan menggunakan metode DPPH yang dinyatakan dengan IC50 sedangkan untuk uji organoleptik dilakukan secara hedonik oleh panelis. Hasil analisis ANOVA menunjukkan bahwa penyimpanan dan penambahan kurma ajwa berpengaruh nyata terhadap aktivitas antioksidan infused water stroberi dan wortel (p<0,05). Aktivitas antioksidan tertinggi diperoleh dari infused water dengan penambahan kurma pada penyimpanan suhu ruang selama 2 jam dengan nilai IC50 sebesar 206,53 ppm yang menandakan aktivitas antioksidan sedang.
Quantity Evaluation of Laactic Acid Bacteria Using the Fncc 0027 and C410li Gene on Different Storage of Acid Bamboo Welkriana, Putri Widelia; Halimatussa'diah, Halimatussa'diah
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.296

Abstract

The fiber content in bamboo shoots is higher than other tropical vegetables. The water content in saw bamboo shoots is quite high, causing the bamboo shoots to have a shelf life of only 2 days. The fermentation process is characterized by the conversion of carbohydrates into lactic acid by bacteria. This study aimed to calculate LAB with FNCC 0027 and C410LI genes in tamarind shoots with different storage periods. The method used in this study is a descriptive method to describe the total plate number of microbes, LAB examination and molecular identification of bacteria found in tamarind shoots with different storage times. The results showed that all samples showed the presence of microbial TPC, examination. As for molecular identification, it was found that samples 1, 2, and 3 were identified as LAB with the C4101LI gene, while the FNCC0027 gene was not detected. Concluded that the total plate number values with different storage times of tamarind shoots, namely 0, 24, and 48 hours were 2.5x105 CFU/g, 5.4x105 CFU/g, and 7.3x105 CFU/g. In samples 1, 2, and 3, there were lactic acid bacteria of the type Bacillus plantarum C401L1.
Analysis of the Relationship Between The Availability of Trained Health Workers and The Tuberculosis Cure Rate in Indonesia Year 2021 Putro, Edi Utomo; Intansari, Intansari; Delfiyanti, Rani; Siregar, Kemal
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.308

Abstract

Tuberculosis is an infectious disease which is one of the ten causes of death in the world. Indonesia is in the third place, in the number of tuberculosis notifications after India and the Philippines in 2019 to 2020. Thus, a global target, the End TB Strategy, was set, with the aim of reducing TB cases by 90% in 2035 and in line with the National TB Management Strategy with a target of reducing incidence TB is 65 cases per 10,000 population in 2030. One of the things that plays a role in achieving the targets is the quality and quantity of trained health workers in the TB program. This study aims to determine the relationship between the availability of trained health workers and the cure rate for tuberculosis treatment in 34 provinces in Indonesia. The method used is quantitative research with a correlation study. The data used is secondary data, analyze with bivariate test and correlation test. Based on statistical tests, it was found that the results of pvalue = 0.00001 and r = 0.68, meaning that there is a significant relationship between the availability of trained health workers and the cure rate for TB treatment with a positive correlation and strong relationship strength.
Efektivitas Madu Dalam Kemasan Terhadap Rasa Tanpa Testis Bakteri Salmonella typhi Darwin, Bastian; Marlina, Melli
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.241

Abstract

Demam tifoid atau typhus abdominalis adalah suatu infeksi akut yang terjadi pada usus halus disebabkan oleh bakteri gram negatif Salmonella typhi yang ditransmisikan melalui makanan maupun minuman yang terkontaminasi oleh feses atau urin dari orang terinfeksi. Madu kemasan adalah madu yang dibuat dari madu murni pilihan yang sebelumnya telah diproses sedemikian rupa secara steril dan higienis kemudian dikemas dengan berbagai ukuran dan harga yang lebih terjangkau dari pada madu alami. Penelitian ini bertujuan untuk mengetahui efektivitas madu kemasan terhadap daya hambat bakteri Salmonella typhi. Jenis penelitian ini dilakukan dalam bentuk eksperimen murni yang dianalisa secara diskriptif kuantitatif dan disajikan dalam bentuk diagram sederhana. Penelitian dilakukan di Laboratorium Mikrobiologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang dengan menggunakan metode disk difusi cakram kertas. Hasil penelitian ini didapatkan rata – rata efektivitas madu kemasan terhadap daya hambat bakteri Salmonella typhi pada konsentrasi 25% tidak terbentuk, 50% sebesar 2,4 mm dan 75% sebesar 5,4 mm dengan uji wilcoxon didapatkan nilai p = 0,006. Kesimpulan dari penelitian ini yaitu madu  kemasan dengan konsentrasi 25%, 50%, dan 75% tidak memiliki efektivitas terhadap daya hambat bakteri Salmonella typhi.
Pengaruh Pencucian Darah Domba dengan NaCl 0,85% Terhadap Pertumbuhan Bakteri Neisseria gonorrhoeae Pada Media CAP Agrijanti, Agrijanti; Intan Suryawatie Yomo, Meidiana; Khusuma, Ari; Kusuma Dewi, Lale Budi
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.259

Abstract

Neisseria gonorrhoeae is a fastidious organism. The growth medium for the bacterial culture of Neisseria gonorrhoeae is a Chocolate Agar Plate (CAP) medium enriched with additional nutrients such as sheep blood, vancomisin, nystatin, and isovitaleX. in general, the making of  CAP  is not washed (defibrinated) the sheep blood, in this research, the sheep blood was washed (defibrinated) with NaCl 0.85%. The purpose of the research is to determine the effect of washed sheep blood with NaCl 0.85% on the growth of Neisseria gonorrhoeae in CAP. The method of this research is a pre-experiment conducted in a laboratory to find out a symptom or effect that arises as a result of the treatment of the sample. The treatment in this research is CAP washed of sheep blood and CAP with unwashed sheep blood as a control. The result is the colonies that grew on CAP treatment were better than colonies that grew on CAP control. The conclusion of this research is there is an effect of washed sheep blood with NaCl 0.85% on the growth of Neisseria gonorrhoeae in CAP medium.
Perbedaan Hasil Pemeriksaan Widal Metode Slide menggunakan Sampel Serum dan Plasma EDTA pada Mahasiswa Teknologi Laboratorium Medik Badriyah, Nurul; Mulyanto, Arif; Wardani, Dita Pratiwi Kusuma; Sulistyowati, Retno
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.283

Abstract

Demam tifoid merupakan penyakit infeksi akut usus halus yang disebabkan oleh bakteri S. typhi dan S. paratyphi. Penyakit demam tifoid termasuk penyakit menular. Penularan disebabkan karena makanan atau minuman yang disajikan tidak bersih dan tingkat higenis individu sanitasi lingkungan. Pemeriksaan widal merupakan suatu pemeriksaan serologis untuk mendeteksi antibodi terhadap bakteri S. typhi yang berdasarkan reaksi aglutinasi antara antigen dengan antibodi yang disebut aglutinin, dengan mengukur aglutinasi titer antibodi terhadap antigen O dan H dalam sampel. Penelitian ini bertujuan untuk mengetahui ada tidaknya perbedaan nilai titer pada pemeriksaan widal yaitu metode slide sampel serum dan plasma EDTA dengan rancangan cros sectional sampel yang digunakan sebanyak 26. Pada Mahasiswa Teknologi Laboratorium Medik. Data yang dihasilkan dianalisis dengan uji univariat dan uji pearson chi square. Hasil penelitian ini didapatkan hasil pada serum terbanyak yaitu pada titer 1/320 (61,5%) sedangkan pada plasma EDTA terbanyak yaitu pada titer 1/80 (46,2%) yang menunjukan nilai p velue 0,0247 (p >0,05), maka berdasarkan hasil tersebut dapat disimpulkan bahwa tidak terdapat perbedaan antara pemeriksaan widal menggunakan sampel serum dan plasma EDTA antigen O dan H.       
Creativity Profile Of Medical Laboratory Technology Students In Medical Instrumentation Learning pauzi, Iswari; Hakim, Aliefmam; Doyan, Aris; Hadiprayitno, Gito; Rokhmat, Joni; Sukarso, A.A
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.322

Abstract

The aim of the study was to describe the creativity profile of students of the Applied Undergraduate Study Program of Medical Laboratory Technology, Health Polytechnic, Ministry of Health, Mataram. The research method used is descriptive research. Data was collected through a questionnaire made using the Google form and then distributed to research respondents totaling 52 students. The data analysis technique used is descriptive analysis using the percentage of each indicator. The results showed that students' critical thinking skills were low on indicators of providing further explanations and drawing conclusions, and creative thinking skills were low for students to think fluently and originally.
Uji Aktivitas Antimikroba Ekstrak Kulit Ranting dan Kayu Sakit Sengon (Falcataria moluccana) dengan Pelarut Metanol dan Etil Asetat Rumidatul, Alfi; Wahyuniah, Bunga; Zamaludin, Deni; Khusna, Wasiyah; Fadhila, Feldha; Maryana, Yayan
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.211

Abstract

Sengon (Falcataria moluccana) memiliki senyawa metabolit yang beragam dan memiliki aktivitas antimikroba baik pada bagian kulit dan daunnya. Penelitian ini bertujuan untuk mengetahui potensi ekstrak Sengon sebagai antimikroba. Metode yang digunakan dalam pembuatan ekstrak adalah maserasi dengan pelarut metanol dan etil asetat dan dibuat menjadi lima varian konsentrasi yaitu 9%, 9,5%, 10%, 10,5%, dan 11% sedangkan untuk pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram atau Kirby bauer terhadap mikroba uji yaitu Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella typhi, Shigella dysenteriae, Klebsiella pneumonia, Escerichia coli, dan Candida albicans. Hasil penelitian menunjukkan konsentrasi optimum ekstrak kulit ranting sakit dengan pelarut metanol yaitu 11% pada S. aureus dengan diameter 8,3 mm, P. mirabilis dengan diameter 4,3 mm, P. aeruginosa dengan diameter 0,5 mm, K. pneumonia dengan diameter 9 mm, dan E. coli dengan diameter 7 mm. Konsentrasi optimum ekstrak kulit ranting sakit dengan pelarut metanol juga terdapat pada konsentrasi 10% dan 11% pada S. dysenteriae dan C. albicans dengan diameter 3 mm, dan pada S. thypi dengan diameter 6 mm. Konsentrasi optimum ekstrak kayu sakit sengon dengan pelarut metanol yaitu 11% pada S. aureus dengan diameter 8,3 mm, P. mirabilis dengan diameter 5,6 mm, dan P. aeruginosa dengan diameter 9 mm. Konsentrasi optimum ekstrak kayu sakit sengon dengan pelarut etil asetat yaitu 10%, 10,5%, dan 11% terhadap C. albicans dengan diameter 2 mm. Hasil yang didapat menunjukkan bahwa ekstrak kulit ranting dan kayu Sengon sakit dengan pelarut metanol dan etil asetat dapat menghambat pertumbuhan mikroba uji dengan konsentrasi optimum ekstrak yang berbeda untuk setiap mikroba uji.
Incidence of Soil Transmitted Helminth (STH) Infection in School-Age Children in the Archipelago of North Sulawesi Lalangpuling, Indra Elisabet; Bawataa, Janiver; Sumenge, Dionysius; Sumampou, Jonas
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.276

Abstract

Archipelagic areas are areas that are still lacking in the application of good sanitation life. One of the infectious diseases related to sanitation is worm infection. This study aims to identify the presence or absence of Soil Transmitted Helminth (STH) intestinal parasitic infection in children in the archipelago. This research was carried out at the Bethel Alo Christian Education Foundation Elementary School, Talaud Regency. This research is a descriptive research. The sampling technique is Porposive sampling, the sample of this research is 35 samples. After microscopic examination using faecal samples, it was found that 5 samples were positive for helminthiasis while 30 other samples were negative. Of the 5 samples, 2 positive samples of Ascaris lumbricoides worm eggs, 2 samples of Trichuris trichiura, and 1 sample of mixed infection of Ascaris lumbricoides and Trichuris trichiura. The results showed as many as 5 positive samples or 14.29% of the 35 samples consisting of 2 samples or 5.71% of Ascaris lumbricoides, 5.71% of Trichuris trichiura, and 1 sample of Mix infection or 2.85%.
Toxoplasmosis Examination With Anti-Toxo IgM And IgG Results In Petshop Staff Pratama, Sandi Setya; Kumoro, Adhi
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.311

Abstract

Toxoplasmosis is an infection caused by a single-celled parasite called Toxoplasma gondii.  Infection is most commonly acquired from contact with cats and their feces or raw or undercooked meat. There are several risk factors that favor the occurrence of this infection including the presence of cats in the residence, cleaning of pet litter boxes, hand washing habits and consumption of raw or undercooked food. Petshop is a retail business that sells a variety of pets and also sells pet food, accessories, animal care and maintenance. The purpose of this study was to determine the exposure to toxoplasmosis with the results of Toxoplasma gondii IgG antibody examination and Toxoplasma gondii IgM antibody in petshop staff.This study is a descriptive study using cross sectional method with 25 respondents from petshop staff. The test method for toxoplasmosis detection used was Rapid (Immunochromatography). The data analysis technique used was univariate analysis to determine the percentage of seropositive results of Toxoplasma gondii IgG antibodies and Toxoplasma gondii IgM antibodies. Based on the results of the study obtained data 11 respondents seropositive anti-toxo IgG, 14 respondents did not have anti-toxo IgG and anti-toxo IgM and there were no respondents with positive anti-toxo IgM in Petshop officers. Therefore it can be concluded that 11 out of 25 respondents have been infected with Toxoplasma gondii.

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