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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
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Perbandingan Kadar Tumor Necrosis Factor Alpha (TNF- α) Pada Tukang Parkir 1-3 Tahun dan >3 Tahun Dengan Metode ELISA Hidayat, Ahmad; Kartika, Aprilia Indra; Fitria, Meutia Srikandi
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.237

Abstract

Polusi udara terdiri atas campuran substansi-substansi kimia dalam bentuk gas dan zat-zat radikal bebas. Radikal bebas yang masuk ke dalam saluran napas secara langsung dapat mengganggu mekanisme pertahanan antioksidan tubuh serta dapat mengakibatkan terjadinya kerusakan atau oksidasi pada lipid, protein dan DNA pada sel. Radikal bebas yang masuk akan di detoksifikasi oleh sel makrofag, neutrofil, eosinofil dan sel epitel. Hasil dari detoksifikasi radikal bebas oleh sistem pertahanan tubuh berupa Reactive Oxygen Species (ROS), yang kemudian memicu pelepasan sitokin-sitokin proinflmasi salah satunya ialah Tumor Necrosis Factor Alpha (TNF-?). Tujuan penelitian ini untuk mengetahui perbandingan kadar TNF-? pada tukang parkir 1-3 tahun dan >3 tahun menggunakan metode ELISA, sampel yang digunakan adalah tukang parkir dengan lama kerja 1-3 tahun sebanyak 10 serum dan >3 tahun sebanyak 10 serum. Hasil penelitian menunjukan bahwa kadar rata-rata TNF-? pada sampel serum tukang parkir dengan lama kerja >3 tahun (1995.0565 pg/ml) lebih tinggi dibandingkan dengan masa kerja 1-3 tahun (1962.7485 pg/ml) sehingga lebih beresiko terkena penyakit paru obstruktif kronis, kanker paru, penyakit jantung koroner, aterosklerosis dan penyakit kardiovaskuler.
Modifikasi Bak Pengecatan pada Pembuatan Sediaan Apusan Darah Tepi Jannah, Miftahul; Ardiyanto, Affan
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.265

Abstract

Pembuatan preparat apusan darah tepi menjadi suatu keterampilan yang harus dimiliki oleh seorang tenaga laboratorium kesehatan. Sediaan apusan darah tepi merupakan bagian yang penting dari rangkaian pemeriksaan hematologi. Proses mengering-anginkan ini merupakan bagian penting yang dilakukan sebelum sediaan apusan darah tepi difiksasi dengan metil alkohol dan  bagian penting setelah selesai proses pengecatan. Lamanya waktu yang dibutuhkan untuk proses kering-anginkan sediaan apusan darah ini yang menjadi latar belakang membuat modifikasi bak pengecatan pada pembuatan sediaan apusan darah.Tujuan penelitian ialah melakukan modifikasi terhadap bak pengecatan pada pembuatan preparat apusan darah tepi dan mengefisienkan waktu pembuatan preparat apusan darah. Penelitian ini merupakan penelitian eksperimen dengan memodifikasi bak pengecatan yang terbuat dari bahan akrilik berukuran 30cm x 10cm x 10cm yang ditambahkan kipas mini berukuran 4 cmx 4cm x1 cm. Untuk menghidupkan kipas mini digunakan power bank dengan pengatur kecepatan. Dilakukan uji fungsi dan uji efektifitas waktu pengeringan. Pada bak pengecatan standar rata-rata waktu pengeringan sediaan apusan darah ialah 6 menit 5 detik, bak pengecatan modifikasi dengan kecepatan 1000 rpm ialah 4 menit 32 detik, kecepatan 2000 rpm ialah 2 menit 14 detik,kecepatan 3000 rpm ialah 1 menit 58 detik dan kecepatan 4000 rpm ialah 1 menit 28 detik. Analisis data dilakukan dengan uji normalitas dan uji bivariat pada perangkat lunak SPSS versi 19. Hasil analisis uji normalitas metode Shapiro-Wilk menunjukkan data tidak berdistribusi normal dan uji bivariat metode Mann-Whitney menunjukkan terdapat perbedaan bermakna waktu pengeringan sediaan apusan darah tepi menggunakan alat modifikasi bak pengecatan sediaan apusan darah tepi. Untuk uji kualitas gambaran sel darah merah dan sel darah putih memiliki kualitas sama menggunakan bak pengecatan standar dengan modifikasi bak pengecatan pada pembuatan sediaan apusan darah tepi.
Description Of C-Reactive Protein (CRP) In Patients With Type 2 Diabetes Mellitus At Mulyorejo Health Center In Malang City Kapero, Febriyanti Wora; Mahtuti, Erni Yohani; Rahmawati, Previta Zeizar
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.320

Abstract

Diabetes Mellitus Type 2 is a metabolic disorder condition characterized by hyperglycemia, due to decreased insulin secretion. Many cytokines play a role in the acute phase response, so that markers such as CRP increase in patients with type 2 DM. The type of research used in this study is descriptive research, which describes the results of CRP examination in patients with type 2 diabetes. Examination of respondents' serum C-Reactive Protein (CRP) levels was carried out qualitatively using the Latex Agglutination method and followed by a semi-quantitative method, namely dilution to determine the level of inflammation. The results of CRP examination on 30 serum samples that have been carried out on patients with Type 2 Diabetes Mellitus found that 29 (96.7%) were positive and 1 (3.3%) of respondents were negative. The group of CRP levels for titer 12 was 5 samples (16.7%), titer 24 was 17 samples (56.7%), titer 48 was 5 samples (16.7%), and titer 96 was 2 samples (6.7). %). The results of the four groups with the highest CRP levels, namely a titer of 24 mg/L as many as 17 samples (56.7%) showed that some of the CRP levels were higher than normal. Conclusion Almost all respondents with type 2 Diabetes Mellitus had positive C-Reactive Protein examination results and a small proportion of type 2 Diabetes Mellitus patients had negative C-Reactive Protein examination results. 
Skrining Penyakit Menular Pada Ibu Hamil Di Rumah Sakit Umum Daerah Kwaingga Kabupaten Keerom Prastyawati, Rini; Romadhonni, Tika; Pradasari, Thiara
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.205

Abstract

A research entitled Infectious Disease Screening for Pregnant Women at the Kwaingga, Keerom Regency. The purpose of this study was to determine the results of Hepatitis B and Syphilis screening in pregnant women at Kwaingga Keerom Regional Hospital. The population in this study were all patients who came for antenatal care at the Kwaingga Keerom District Hospital. The sample in this study was the blood of pregnant women used as serum with a total sample of 30 samples. The examination method is the immunochromatography method. This screening was carried out for 1 month, from January 20 to February 25, 2020. HBsAg and TP Rapid examination results obtained for HBsAg as many as 7% positive infected with Hepatitis B and 93% other negative results, and the results of TP Rapid examination were obtained results as much as 3% positive for syphilis and 97% were negative. so, from the results of HBsAg and TP Rapid examination, positive samples of hepatitis B and syphilis were still obtained in pregnant women at Kwaingga Keerom Regional Hospital.
Pengaruh Konsumsi Tuak Terhadap Kadar Asam Urat Pada Masyarakat Dewasa di Desa Jagaraga Timur Juliantini, Ni Komang; Fihiruddin, Fihiruddin; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.256

Abstract

Tuak is a type of alcoholic drink made from palm sugar. Excessive consumption of tuak continuously can increase the levels of uric acid in the body. Uric acid is the end product of purine metabolism. This study aimed to determine the effect of tuak consumption of uric acid levels in the adult community in East Jagaraga Village. This research is an analytical observation using a cross-sectional approach. The sampling technique was a simple random sampling of 48 people, namely 24 adults that consumed tuak and 24 adults who did not consume tuak. Data analysis in SPSS used an independent sample t-test. The average uric acid levels in 24 respondents that consumed and did not consume tuak were 7.46 mg/dl and 4.92 mg/dl, respectively. The results show that there is an effect of tuak consumption uric acid levels in the adult community in the East Jagaraga Village, this is shown in a probability value (p = 0.000) <0.05 then Ho is rejected and Ha is accepted. From these results, it can be concluded that the consumption of tuak in the long term affects increasing uric acid levels.
Analisis Motivasi Pendonor Darah di PMI Kabupaten Sleman Yogyakarta Mufidah, Hartalina; Kristanti, Handriani; Khristiani, Eva Runi
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.272

Abstract

Blood donation is an essential element in blood transfusion. The Indonesian Red Cross of Sleman Regency (PMI) is one of the Blood Donor Units in the Province of D.I. Yogyakarta, but only 1% of the population in Sleman became blood donors. Blood donation motivation is closely related with knowledge, attitude, and behavior. This study aims to determine the motivational factors for blood donation at PMI Sleman Regency. This research used a cross-sectional study design which was carried out at PMI Sleman in January-December 2021. The sample of this study was 100 respondents who were determined using the accidental sampling technique. Analysis of research data was carried out univariate and bivariate with Fisher's exact test. The results of this study indicate that age (p-value 0.901>0.05), gender (p-value 0.597>0.05), education (p-value 0.914>0.05), occupation (p-value 0.797>0.05), knowledge (p-value 0.097>0.05), and attitudes (p-value 0.393>0.05) didn’t have relationship with blood donor motivation, but there was relationship between behavior and motivation about blood donation with p-value 0.009<0 ,05.  Based on the results of this study, it can be concluded that knowledge, attitudes, and behavior are motivational factors for blood donors, but behavior is a significant factor in blood donor motivation in PMI, Sleman Regency, Yogyakarta. Increasing routine blood donation activities and educating the public about the value of blood donation could create good behavior for voluntary blood donors
Effects of Carbohydrate Diet Programs on Urine Ketones Positiveness with Long Time on a Diet 1 Year, 2 Years and 3 Years Maya, Dyah Astining; Pauzi, Iswari; Zaetun, Siti; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.303

Abstract

Carbohydrate diet is a diet pattern that reduces carbohydrate consumption. Therefore, the body will use fat as an alternative energy source. The continuous use of fat causes the accumulation of fatty acids so that it often causes ketone positivity in the urine. Objective : Find out the effect of a carbohydrate diet program on the positivity of urine ketones. Method : This research is an analytic observational study with a cross sectional approach. The sample used was urine from respondents who had been on a carbohydrate diet for 1 year, 2 years and 3 years, then urine ketones were examined using the dipstick method. Data analysis was carried out using the Chi-Square test with a confidence value (α = 0.05). Result : The sample in this study amounted to 45 samples, with a total of 23 samples of positive ketones and 22 samples of negative ketones. The results of the Chi Square test obtained an Asymp. sig value of 0.009 <0.05 (α) which indicates that the carbohydrate diet program has a significant effect on the positivity of urine ketones.
Description of SGPT Levels in Construction Workers Who Have an Active Smoking Habit Sapna, Eva; Inayati, Nurul; Jiwantoro, Yudha Anggit; Kristinawati, Erna
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.349

Abstract

Physical activity is body movement that requires skeletal muscles and can cause energy expenditure. Physical activity is divided into three, namely light, moderate and heavy physical activity. Construction workers who carry out heavy physical activity for 7 to 8 hours every day can lose a lot of energy and fluids as well as important micronutrients, one of which is iron. Apart from carrying out heavy physical activities, construction workers also have an active smoking habit, which means they are considered active smokers if they smoke more than 20 cigarettes every day. Smoking is something that can cause health problems and smoking has become the biggest cause of death in the world. Cigarette raw materials such as tar, nicotine and carbon monoxide are the main toxicants that can trigger the formation of free radicals. The Research objective is to determine the description of SGPT levels in construction workers who have an active smoking habit. The methods of this research design is descriptive observational research where this research was carried out using the enzymatic kinetic method a total of 28 samples based on calculations using the Slovin formula. The results of the research is examining SGPT levels in construction workers from 28 samples showed an increase in active smokers 11-15 years, namely 3 samples and active smokers 16-20 years, namely 2 samples, there was an increase or were outside the normal value, which is where the normal value of SGPT levels in men -men, namely 42 U/L. The conclusion of this research is showed that 5 of the 28 samples had increased SGPT levels with levels of 63 U/L, 58 U/L, 52 U/L and 49 U/L in 2 samples.
Faktor Koreksi Nilai Laju Endap Darah (LED) Pada Penderita Tuberkulosis Menggunakan Metode Westergren dan Wintrobe Dewi, Rizki Amalia; Zaetun, Siti; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.215

Abstract

Pemeriksaan LED bermanfaat untuk memantau perjalanan penyakit dan memantau keberhasilan terapi penyakit kronik misalnya arthritis rheumatoid dan tuberculosis. Metode pemeriksaan LED diantaranya metode westergren dan wintrobe, kedua metode ini merupakan cara manual. Tujuan penelitian pada penelitian ini untuk mengetahui faktor koreksi nilai laju endap darah pada penderita tuberkulosis menggunakan metode westergren dan wintrobe. Metode penelitian yang digunakan pada penelitian ini bersifat pre-eksperimen dengan 2 kelompok perlakuan LED yaitu diuji dengan metode westergren dan wintrobe. Hasil penelitian  didapatkan jumlah hasil pemeriksaan LED metode westergren 622 mm/jam dengan rerata 38,875 mm/jam dan jumlah hasil pemeriksaan LED metode wintrobe 520 mm/jam dengan rerata 32,5 mm/jam. Rentang nilai metode westergren 12-115 mm/jam, rentang nilai metode wintrobe 18-47 mm/jam kemudian hasil pemeriksaan LED dari metode westergren dan wintrobe dikalikan dengan faktor koreksi kemudian hasil perkalian akan dimasukkan dalam rentang nilai masing-masing metode, metode yang hasil perhitungannya lebih banyak berada dalam rentang nilai adalah metode yang lebih baik untuk melakukan pemeriksaan LED. Uji normalitas dan homogenitas tidak berdistribusi normal dan tidak homogen, maka dilanjutkan dengan uji statistik Mann Whitney. Dari tabel hasil analisis Mann Whitney menunjukkan nilai p 0,895 > 0,05. Karena nilai p lebih besar dari α 0,05 maka dapat disimpulkan bahwa tidak terdapat perbedaan yang bermakna antara dua kelompok dengan begitu Ha ditolak. Kesimpulan dari penelitian ini metode westergren adalah metode yang paling baik digunakan untuk melakukan pemeriksaan LED dari pada metode wintrobe karena hasil pemeriksaan metode westergren yang telah dikalikan dengan faktor koreksi lebih banyak berada dalam rentang nilai yaitu 15 hasil perkalian (93,75%).
Pengaruh Hemolisis Terhadap Nilai Trombosit Dengan Menggunakan Metode Direct Counting Nugrahena, Nurmalita Putri; Sudarsono, Tantri Analisawati; Wijayanti, Linda
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.228

Abstract

Hemolisis yaitu pecahnya sel darah merah dan keluarnya hemoglobin ke plasma. Hemolisis dapat terjadi secara in vitro dan in vivo. Sampel yang hemolisis dapat mempengaruhi hasil pemeriksaan laboratorium yang dapat menyebabkan kesalahan diagnosis, terapi dan prevensi. Tujuan penelitian ini adalah untuk melihat pengaruh hemolisis terhadap nilai trombosit dengan menggunakan metode penghitungan serta penilaian trombosit dari sampel darah yang tidak lisis dan dilisiskan secara in vitro. Penelitian ini dilakukan secara eksperimental pada bulan Juli 2020 di Laboratorium Patologi Klinik Fakultas Kedokteran Universitas Muhammadiyah Purwokerto. Teknik pengambilan sampel dilakukan secara purposive sampling sebanyak 14 sampel.Perhitungan nilai trombosit dilakukan menggunakan metode penghitungan langsung.  

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