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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
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Efektivitas Penggunaan Cuka Apel (Apple Cider Vinegar) Terhadap Daya Hambat Pertumbuhan Staphylococcus Aureus Yang Diisolasi Dari Ulkus Diabetes Mellitus Novianty, Alfiah; Agrijanti, Agrijanti; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.200

Abstract

Diabetic ulcers are a chronic complication of Diabetes Mellitus in the form of open sores on the surface of the skin. Staphylococcus aureus bacteria is one of the bacteria that causes infection in wounds. Staphylococcus aureus bacteria will flourish in areas that have infections. An increasingly severe infection will cause gangrene. As it is known that Apple vinegar contains chemicals that are as antibacterial. Therefore apple vinegar can be used as a treatment in inhibiting the growth of Staphylococcus aureus bacteria. This study used a quasi-experimental design with 6 treatments, namely Tetracycline Antibiotics as a negative control of growth, sterile aquadest as a positive control of growth and apple vinegar with concentrations of 100%, 50%, 25%, and 12.5%. Then performed a statistical test using Kruskal Wallis with a confidence level of 95% or α = 0.05. Result: Sample Diabetic Foot Ulcer positively contained Staphylococcus aureus. Test the sensitivity that is done by using vinegar apple effective to inhibit the growth of Staphylococcus aureus at concentrations of 50% with a power resistor of 21.75 mm and a concentration of 100% by 24 mm. Conclusion: Apple Cider Vinegar effective to inhibit the growth of Staphylococcus aureus was isolated from Ulcer Foot Diabetic.
Efektifitas Penggunaan Platelet Agitator Terhadap Jumlah Trombosit Di UTD PMI Kota Surabaya Tahun 2019 Widuri, Sasi; Dhana Reswari, Putu Ayu; Nuraeni, Faradila
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.244

Abstract

Selama penyimpanan, aktivitas metabolic dari trombosit dan residual leukosit berlanjut untuk mengkonsumsi nutrient dan menghasilkan produk metabolik yang berbahaya. Aktivasi faktor pembekuan, celular debris, dan enzim proteolitik ditemukan dalam plasma suspensi yang berefek kurang baik terhadap trombosit. Banyak perubahan structural pada sitoskeleton trombosit dan antigen di membrane permukaan selama penyimpanan dan berhubungan dengan buruknya recovery dan survival post transfusi in vivo. Tes in vitro yang digunakan untuk menilai perubahan pada trombosit umumnya hanya jumlah trombosit, volume konsentrat, pH selama 5 hari, dan leukosit. Komponen Trombosit harus disimpan pada Platelet Agitator dengan suhu penyimpanan 22oC ± 2oC. Penelitian ini dilaksanakan di UTD PMI Kota Surabaya. Untuk melihat efektifitas penyimpanan komponen Trombosit pada Platelet Agitator. Berdasarkan hasil uji mutu internal untuk produk TC di UTD PMI Kota Surabaya pada tahun 2019 dari 30 kantong darah. Penelitian ini dilaksanakan selama 3 bulan pada bulan Januari – April 2019. Hasil penelitian : Kadar Trombosit mengalami kenaikan kualitas baik pada produk komponen darah Trombosit sebanyak 15 kantong darah (100%) dan mengalami penurunan kualitas kadar trombosit kurang baik sebelum menggunakan alat platelet agitator sebanyak 6 kantong darah (40%). Kesimpulan :Kadar Trombosit setelah menggunakan alat platelet agitator terdapat 15 kantong darah (100%) yang memenuhi spesifikasi. 
Kadar Ureum, Kreatinin, Serum Iron (SI) dan Total Iron Binding Capacity (TIBC) pada Pasien Chronic Kidney Disease (CKD) sebelum dan setelah Hemodialisis Ariami, Pancawati; Zaetun, Siti; Gunaifi, Aan; Diarti, Maruni Wiwin
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.279

Abstract

Tujuan  penelitian ini adalah untuk mengetahui kadar ureum, kreatinin, SI (serum iron) dan TIBC dalam darah sebelum dan sesudah hemodialisis. Jenis penelitian ini obervasional deskriptif.  Populasi dalam penelitian ini adalah keseluruhan pasien CKD RSUD Kota Mataram yang melakukan Hemodialisis. Sampel dalam penelitian ini menggunakan serum  pasien CKD yang melakukan Hemodialisis. Besar sampel 46 responden, Data  yang dikumpulkan kadar ureum, kreatinin, SI (serum iron) dan TIBC pada pasien CKD di RSUD Kota Mataram dianalis menggunakan uji Wilcoxon signed Rank Test pada tingkat kepercayaan 95% p α = 0,05. Hasil penelitian menunjukkan rerata kadar ureum, kreatinin, SI (serum iron) dan TIBC pada pasien CKD sebelum melakukan  hemodialisis berturut-turut sebesar 133,3 mg/dL ; 8,86 mg/dL ; 65,2 µg/dL ; dan 259,7 µg/dL. Rerata kadar ureum, kreatinin, SI (serum iron) dan TIBC pada pasien CKD setelah melakukan  hemodialisis berturut-turut sebesar 66,6 mg/dL ; 4,51 mg/dL ; 40,4 µg/dL ; dan 203,8 µg/dL. Kesimpulan hemodialisis berpengaruh terhadap kadar ureum, kreatinin, SI (serum iron) dan TIBC pada pasien CKD.
Morphological Analysis and Biochemical Properties of Staphylococcus aureus Cultures Grown on Alternative Media Peanut Flour (Arachis Hypogaea L.) Jiwantoro, Yudha Anggit; Putri, Salmania
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.305

Abstract

Sources of nutrition especially sufficient protein, is needed for the growth of Staphylococcus aureus bacteria. Synthetic media is the most common medium for growing Staphylococcus aureus. Peanut is a biological source in nature with a higher protein content than synthetic materials which can be used as an alternative. Objective to determine the morphology and biochemical properties of Staphylococcus aureus bacteria grown on alternative media of peanut flour (Arachis hypogaea L.) as a medium for bacterial growth. This is a True-Experiment study with a completely randomized design. The experimental unit consists of five variations in the concentration of peanut alternative media. The sampling technique was done by simple random sampling. Data analysis used Descriptive. Staphylococcus aureus bacteria can grow at all concentrations of alternative media of peanut flour. The colony size at concentration of 2% was very small with weakly fermented mannitol. Meanwhile a concentration of 3% to 5% produces moderately fermented mannitol. The concentration of 6% is the concentration with the most widely fermented mannitol. The biochemical tests of all concentrations yielded positive catalase and coagulase, and the sugar test yielded positive reactions. An alternative medium of peanut flour can be used as a medium for the growth of Staphylococcus aureus bacteria with the most effective concentration of 6%.
Analysis of Patient Examination Results with Clinical Symptoms of Gonorrhea Using Microscopic Examination at Puskesmas Dasan Agung Widyastini, Ni Komang Ari Santi; Zaetun, Siti; Agrijanti, Agrijanti; Rohmi, Rohmi
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.345

Abstract

Tropical clinical symptoms of gonorrhea occur in men, to confirm a clinical diagnosis then use microscopic examination with gram coloring. The Puskesmas of Dasan Agung became a reference to the inspection of the STI in the city of Mataram. The purpose of this study is to identify clinical symptoms and results of microscopic examination using gram coloring. This type of research is descriptive observational which aims to know or describe the picture of a disease based on direct observation objectively. The population in this study were all patients who came with symptoms that lead to sexually transmitted diseases in January - March, totaling 13 respondents. Sampling was done by non-random purposive sampling method. This research was conducted at the Dasan Agung Health Center laboratory. The results of a study of 13 patients with clinical symptoms of STI obtained 13 positive respondents (100%) identified Neisseria gonorrhoeae. 10 male patients (67.9%) and 3 female patients (23.1%) were identified with heterosexual sexual orientation in 10 cases and 3 were homosexual.
Aktivitas Antibakteri Ekstrak Biji Areca catechu L. Dalam Menghambat Pertumbuhan Salmonella typhi Selpiah, Mega; aini, aini; Ustiawaty, Jumari
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.210

Abstract

Demam tifoid merupakan penyakit yang disebabkan oleh infeksi bakteri Salmonella typhi yang merupakan penyakit endemik yang dapat menyerang banyak orang dan masih menjadi masalah kesehatan di daerah tropis terutama di negara-negara berkembang termasuk Indonesia. Berbagai upaya pencegahan, pengobatan dan terapi yang telah dilakukan untuk mengurangi permasalahan infeksi bakteri. Alternatif baru yang terus diteliti dan dikembangkan adalah pengobatan menggunakan tanaman herbal. Salah satu tanaman yang dapat dimanfaatkan sebagai alternatif dalam pengobatan infeksi bakteri adalah tumbuhan pinang (Areca catechu L.) yang memiliki kandungan senyawa sebagai antibakteri seperti alkaloid, flavonoid dan tanin. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak biji buah pinang (Areca catechu L.) dalam menghambat pertumbuhan Salmonella typhi, untuk menentukan Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) ekstrak biji buah pinang (Areca catechu L.) dalam menghambat pertumbuhan Salmonella typhi. Metode yang digunakan dalam penelitian ini adalah dilusi cair yang digambarkan sebagai efek bakteriostatik dan dilusi padat yang digambarkan sebagai efek bakterisidal. Jenis penelitian ini adalah eksperimental laboratories dengan desain the post only control group desain dengan menggunakan analisa data uji statistik versi 16. Hasil penelitian ini adalah ekstrak biji buah pinang (Areca catechu L.) mampu menghambat pertumbuhan Salmonella typhi. Konsentrasi Hambat Minimum (KHM) ekstrak biji buah pinang (Areca catechu L.) didapatkan pada konsentrasi 1%. Konsentrasi Bunuh Minimum (KBM) ekstrak biji buah pinang (Areca catechu L.) tidak didapatkan karena pada semua media Nutrient agar terdapat pertumbuhan bakteri. Adanya pertumbuhan bakteri tersebut memberikan makna bahwa ekstrak biji buah pinang (Areca catechu L.) hanya mampu menghambat (bakteriostatik) namun tidak mampu membunuh 
Potensi Air Kelapa Muda Dan Air Kelapa Obat Terhadap Pertumbuhan Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) Dengan Metode Dilusi Prabowo, Finda Rizky Putri; Mujahid, Ikhsan; Mulyanto, Arif
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.235

Abstract

Methicillin-Resistant Staphylococcus aureus merupakan strain S. aureus yang telah resisten terhadap aktivitas antibiotik golongan β-laktam, termasuk golongan penicillinase-resistant penicillins (oxcacillin, methicillin, nafcillin, cloxacillin, dicloxacillin), cephalosporin dan carbapenem. Kelapa yang sering dimanfaatkan sebagai obat yaitu kelapa muda kulit hijau dan kelapa obat atau sering disebut dengan kelapa wulung yang ditandai dengan bagian mesokarp (sabut) berwarna merah muda. Kelapa wulung memiliki khasiat untuk mengobati berbagai jenis penyakit. Tujuan penelitian ini adalah untuk mengetahui kemampuan air kelapa muda dan air kelapa obat dalam mempengaruhi pertumbuhan bakteri Methicillin-Resistant Staphylococcus aureus (MRSA). Penelitian ini dilakukan secara true eksperimental dengan rancangan pre test post test control group design. Terdiri dari 6 kelompok dengan 2 kelompok sebagai kontrol positif dan kontrol negatif serta 4 kelompok diberi perlakuan air kelapa muda kulit hijau, air kelapa muda kulit coklat, air kelapa obat kulit hijau, dan air kelapa obat kulit coklat. Jumlah bakteri sebelum dan sesudah perlakuan dihitung secara langsung menggunakan haemocytometer dan tidak langsung ditumbuhkan pada medium MHA. Penelitian ini dilakukan dari bulan Juli-Oktober 2020. Data dianalisis dengan uji repeated ANOVA. Hasil penelitian menunjukkan adanya pengaruh air kelapa terhadap jumlah bakteri MRSA dengan metode langsung, metode tidak langsung, dan perbandingan antara metode langsung dan tidak langsung. Air kelapa obat kulit coklat paling baik dalam menghambat pertumbuhan bakteri MRSA dengan penurunan jumlah bakteri 8,2 x 105 ± 5,27 x 105 CFU/mL sebelum perlakuan menjadi 1,7 x 105 ± 9,01 x 104CFU/mL setelah perlakuan pada metode tidak langsung.
Analisi Kandungan Timbal (Pb) Pada Air Sumur Di Sekitar Tempat Pembuangan Akhir Kalipancur Kabupaten Purbalingga Heny, Dewi Kristiya Nugra; Dhanti, Kurnia Ritma; Wardani, Dita Pratiwi Kusuma
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.224

Abstract

Air memiliki peran penting dalam memenuhi kebutuhan manusia sebagai sumber air minum. Salah satu penyebab pencemaran air adalah logam berat. Tujuan penelitian ini adalah menganalisis kandungan timbal (Pb) pada air sumur di sekitar TPA Kalipancur Kabupaten Purbalingga. Penelitian ini dilakukan secara observasional deskriptif pada bulan Agustus 2020 di Laboratorium Kimia Analisis dan Biologi Farmasi Fakultas Farmasi Universitas Muhammadiyah Purwokerto. Teknik pengambilan sampel dilakukan secara purposive sampling. Sebanyak 10 sampel air sumur diambil dengan radius 100-500 m dari TPA Kalipancur. Kadar timbal (Pb) diukur menggunakan metode Atomic Absorption Spectrophotometer (AAS) dan dianalisis secara deskriptif numerik. Hasil penelitian menunjukkan kandungan timbal (Pb) pada seluruh sampel air sumur di sekitar TPA Kalipancur Kabupaten Purbalingga sebesar <0,01 mg/L. 
Differences in Results of Examination of Urine Protein Levels Dipstik Method with 6% Acetic Acid Method in Patients with Diabetes Mellitus in Area Puskesmas Baturraden II Rahayu, Feni Putri; Sulistiyowati, Retno ,; Sudarsono, Tantri Analisawati; Rahaju, Minto
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.291

Abstract

Diabetes Mellitus (DM) is a non-communicable disease and a major public health issue in Indonesia and around the world. Proteinuria caused by diabetes in the blood vessels can cause kidney damage. Diabetes can also cause nerve damage in the body. This can make urinating difficult. The pressure caused by a full bladder can harm the kidneys. If urine remains in the bladder for a longer duration, it can cause an infection due to the rapid growth of bacteria in urine with a high sugar level, even if the presence of protein in the urine has been tested in a laboratory. Objective: The study aimed to determine the difference in the results of urine protein examination with the dipstick method with the 6% acetic acid method in DM patients in the working area of Puskesmas (Public Health Center) Baturraden II. Methods: The research design used was analytic observational with a cross-sectional study. Thirty urine samples were used with purposive sampling as the sampling technique. Chi-Square Test was employed for Statistical Analysis. The results of the tests produced a significant value of 0.000 (P 0,0 5), indicating that the p-value was lower in relation to the degree of error. It could be concluded that the results of urine protein examination using the dipstick method and the 6% acetic acid method differed in DM patients.
The Relationship Between Blood Glucose Levels To The Results Mycobacterium Tuberculosis Examination In Tuberculosis Patients Puspita, Ria; Suliati, Suliati; Mutiarawati, Diah Titik
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.330

Abstract

Tuberculosis (TB) is still high. 10.4 million new cases of TB occur and 1.4 million die from the disease is found The World Health Organization (WHO). Elevated blood glucose can lead to more severe tuberculosis infection, reactivation of dormant tuberculosis foci, and poor treatment outcomes. Determine the relationship of blood glucose levels to the results of Mycobacterium tuberculosis examination in suspected tuberculosis patients in Ketapang Banyuwangi area is the purpose of this of research study. The method was correlational analytic design of this study. The results conducted on 118 people who performed tuberculosis screening examinations, 32 people who qualified as a population. And 30 (thirty) people who meet the inclusion requirements as research samples. The results of the patient's molecular rapid test showed 6 (six) people were positive (+), and the results of the patient's culture showed 3 (three) people were positive (+) and 3 (three) other people were negative (-). While the results of fasting blood glucose level examination in 3 (three) tuberculosis patients conducted periodically (every week) for 5 (five) weeks were in the range of 78mg/dL - 150mg/dL. The conclusion is that there is a significant relationship between blood glucose levels and results of Mycobacterium tuberculosis examination of tuberculosis patients in the first, second and third patients in Ketapang Banyuwangi area.

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